Reviewed, UniProtKB/Swiss-Prot Q7U350 (CCA_BLOFL)

Names and origin

Protein names

Recommended name:
    CCA-adding enzyme
    EC=2.7.7.25
    EC=2.7.7.21
Alternative name(s):
    tRNA nucleotidyltransferase
    tRNA adenylyl-/cytidylyl- transferase
    tRNA CCA-pyrophosphorylase
    tRNA-NT

Gene names

Name:	cca
Ordered Locus Names:	Bfl062

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity.
Catalytic activity	ATP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262 CTP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262
Cofactor	Magnesium By similarity.
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.

Ontologies

Keywords
Biological process	RNA repair tRNA processing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA addition Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP tRNA adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	CCA-adding enzyme HAMAP MF_01262		PRO_0000139020
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	23	1	Magnesium By similarity
	Binding site	8	1	ATP or CTP; via amide nitrogen By similarity
	Binding site	11	1	ATP or CTP By similarity
	Binding site	91	1	ATP or CTP By similarity
	Binding site	137	1	ATP or CTP By similarity
	Binding site	140	1	ATP or CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U350-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: DFEB8AF01081EA04	FASTA	411	47,516
	10 20 30 40 50 60 MKKYLVGGAV RDSLLNLPIT EKDWVITGSS AQEMLSIGYE QVGKDFPVFL HPKSHEEYAL 70 80 90 100 110 120 ARTERKLGSG YTGFICHTEP SITIEEDLYR RDLTINAMAY DMNGNLLDPY NGQKDIQLRL 130 140 150 160 170 180 LRHVSNAFYE DPLRVLRVAR FAAKLKNIGF TIAIETFEIM THMIHELKSL SPERVWMETK 190 200 210 220 230 240 KALITDNPQV YFQVLKKCGA LKILFPELDI LFTIPQCTTH HSNLMNLGNQ TMTRLSNISC 250 260 270 280 290 300 LSNDLAIRYA ILCCNLGSEM NPRKQPLKQL TQKKPQISII NNLCNRLKVP NNILKLTKIV 310 320 330 340 350 360 FMYYHDLYDV TKLSSEMIMT IFQAFDCWRT PTRIELIIKI NQSQLLGSKF HINYLFFQST 370 380 390 400 410 LLRTAFNETT QIKANDIINS GFSGINISQE LYSRRLHVLK HWKNKFLTHK Q

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83587.1.
RefSeq	NP_878374.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499262.
GenomeReviews	Gene locus Bfl062 in contig BX248583_GR.
KEGG	bfl:Bfl062.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7U350.
OMA	Q7U350. NPHVYFQ.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL062-MON.
Family and domain databases
HAMAP	MF_01262. [Tree]
InterPro	IPR012006. CCA_bact. IPR002646. PolyA_pol_reg. [Graphical view]
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000813. CCA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCA_BLOFL

Accession

Primary (citable) accession number: Q7U350

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2003
Last modified:	May 5, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)