Reviewed, UniProtKB/Swiss-Prot Q7U345 (TRMD_BLOFL)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Bfl175

Organism

Blochmannia floridanus [Complete proteome] [HAMAP]

Taxonomic identifier

203907 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › ant endosymbionts › Candidatus Blochmannia

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060335
Regions
	Region	134 – 139	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	114	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U345-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 4000DCEA7E6021ED	FASTA	248	28,488
	10 20 30 40 50 60 MLLGIITLFP DMFNAITRYG VVGRSVRKGG LVIKIWNPRD FTYDQYHKVD DRPYGGGVGM 70 80 90 100 110 120 IMMIQPLKRA INQAKNDLGC DAKVIYLSPQ GKRLCQKYVY DLAYNNQALI LVCGRYQGID 130 140 150 160 170 180 ERLIKMEIDE EWSIGDYVLS GGELASMVLI DAMARVLPGT LKNRNSQKSD SFFENKLDCP 190 200 210 220 230 240 YYTRPKIYEG MQVPSVLLSG NHRDINKWRL KYALGNTWIK RPDLLKKIQL TQEEQLLLTE FKNEYLSG

References

[1]

"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes." Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F., Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B., van Ham R.C.H.J., Gross R., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003) [PubMed: 12886019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248583 Genomic DNA. Translation: CAD83694.1.
RefSeq	NP_878478.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1499375.
GenomeReviews	Gene locus Bfl175 in contig BX248583_GR.
KEGG	bfl:Bfl175.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7U345.
OMA	Q7U345. HRSVDDT.
Enzyme and pathway databases
BioCyc	CBLO203907:BFL175-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BLOFL

Accession

Primary (citable) accession number: Q7U345

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)