Reviewed, UniProtKB/Swiss-Prot Q7U337 (FABH_HAEDU)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3
    EC=2.3.1.180
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III
    Beta-ketoacyl-ACP synthase III
      Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	HD_0774

Organism

Haemophilus ducreyi [Complete proteome] [HAMAP]

Taxonomic identifier

730 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_0000110431
Regions
	Region	244 – 248	5	ACP-binding By similarity
Sites
	Active site	112	1	By similarity
	Active site	243	1	By similarity
	Active site	273	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U337-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: C93D729F02EA8FAA	FASTA	316	33,770
	10 20 30 40 50 60 MYSKILATGS YLPKQVRSNA DLEKMVDTTD EWIFTRSGMK ERRIAAADET VATMGAAAAH 70 80 90 100 110 120 KACEMAAIDV NEIELIVVAT TTHSHAYPSA ACQIQGMLAI EDAIAFDVAA ACTGFIYALS 130 140 150 160 170 180 VADQFIRAGK VKTAFVVGAD VNSRVLDEAD RGTVVLFGDG AGAVILQASE QAGILSTHLH 190 200 210 220 230 240 SSADTDNMLA LPIQERGNAQ SGFIQMQGNA TFKLAVSRLA NVVEETLQAN NLQKADLDWL 250 260 270 280 290 300 VPHQANIRII AATAKKLEME MSQVVLTVEK YGNNSAATVP VALDEAVRDG RIQRGQLLLL 310 EAFGGGWTWG SGLVRF

References

[1]

"The complete genome sequence of Haemophilus ducreyi." Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R., Johnson L., Nguyen D., Wang J., Forst C., Hood L. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 35000HP / ATCC 700724.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017143 Genomic DNA. Translation: AAP95679.1.
RefSeq	NP_873290.1.
3D structure databases
SMR	Q7U337. Positions 1-316.
ModBase	Search...
Genome annotation databases
GeneID	1490726.
GenomeReviews	Gene locus HD_0774 in contig AE017143_GR.
KEGG	hdu:HD0774.
NMPDR	fig|233412.1.peg.655.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	TSADRGI.
Enzyme and pathway databases
BioCyc	HDUC233412:HD_0774-MONOMER.
BRENDA	2.3.1.180. 265031.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_HAEDU

Accession

Primary (citable) accession number: Q7U337

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)