Q7U2S5 (ECH1_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable enoyl-CoA hydratase 1
EC=4.2.1.17

Gene names

Ordered Locus Names:

Mb0135

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in the hydration of fatty acids for production of polyhydroxylalkanoates By similarity. UniProtKB P96807
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. UniProtKB P96807
Subunit structure	Homodimer By similarity. UniProtKB P96807
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family. Contains 1 MaoC-like domain.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Probable enoyl-CoA hydratase 1		PRO_0000262760
Regions
	Domain	11 – 132	122	MaoC-like
	Region	60 – 63	4	Substrate-binding By similarity UniProtKB P96807
	Region	86 – 89	4	Substrate-binding (in homodimeric partner) By similarity UniProtKB P96807
	Region	97 – 99	3	Substrate-binding By similarity UniProtKB P96807
	Compositional bias	11 – 14	4	Poly-Ala
Sites
	Binding site	124	1	Substrate; in homodimeric partner By similarity UniProtKB P96807
	Binding site	148	1	Substrate; in homodimeric partner By similarity UniProtKB P96807
	Site	40	1	Important for catalytic activity By similarity
	Site	42	1	Transition state stabilizer Potential
	Site	45	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U2S5 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7A2613466BBFCD57	FASTA	151	16,007
	10 20 30 40 50 60 MRTFESVADL AAAAGEKVGQ SDWVTITQEE VNLFADATGD HQWIHVDPER AAAGPFGTTI 70 80 90 100 110 120 AHGFMTLALL PRLQHQMYTV KGVKLAINYG LNKVRFPAPV PVGSRVRATS SLVGVEDLGN 130 140 150 GTVQATVSTT VEVEGSAKPA CVAESIVRYV A

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248334 Genomic DNA. Translation: CAD92996.1.
RefSeq	NP_853802.1. NC_002945.3.
3D structure databases
ProteinModelPortal	Q7U2S5.
SMR	Q7U2S5. Positions 2-150.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000014723; EBMYCP00000014558; EBMYCG00000014720.
GeneID	1090816.
GenomeReviews	Gene locus Mb0135 in contig BX248333_GR.
KEGG	mbo:Mb0135.
PATRIC	18001968. VBIMycBov88188_0153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016701.
HOGENOM	HBG654657.
OMA	IPFWGEL.
ProtClustDB	CLSK790285.
Enzyme and pathway databases
BioCyc	MBOV233413:MB0135-MONOMER.
Family and domain databases
InterPro	IPR002539. MaoC_deHydtase. [Graphical view]
Pfam	PF01575. MaoC_dehydratas. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECH1_MYCBO

Accession

Primary (citable) accession number: Q7U2S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program