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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. no protein annotated in this organism
  4. o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMenaquinone biosynthesis
LigandMagnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-576-MONOMER
UniPathwayiUPA00079
UPA01057; UER00164

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:BQ2027_MB0570
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003417751 – 5542-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseAdd BLAST554

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7U1S4
SMRiQ7U1S4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218359
OMAiIFRILPG

Family and domain databases

HAMAPiMF_01659 MenD, 1 hit
InterProiView protein in InterPro
IPR004433 MenaQ_synth_MenD
IPR029061 THDP-binding
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
PANTHERiPTHR42916 PTHR42916, 1 hit
PfamiView protein in Pfam
PF02776 TPP_enzyme_N, 1 hit
PIRSFiPIRSF004983 MenD, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00173 menD, 1 hit

Sequencei

Sequence statusi: Complete.

Q7U1S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPSTTQARV VVDELIRGGV RDVVLCPGSR NAPLAFALQD ADRSGRIRLH
60 70 80 90 100
VRIDERTAGY LAIGLAIGAG APVCVAMTSG TAVANLGPAV VEANYARVPL
110 120 130 140 150
IVLSANRPYE LLGTGANQTM EQLGYFGTQV RASISLGLAE DAPERTSALN
160 170 180 190 200
ATWRSATCRV LAAATGARTA NAGPVHFDIP LREPLVPDPE PLGAVTPPGR
210 220 230 240 250
PAGKPWTYTP PVTFDQPLDI DLSVDTVVIS GHGAGVHPNL AALPTVAEPT
260 270 280 290 300
APRSGDNPLH PLALPLLRPQ QVIMLGRPTL HRPVSVLLAD AEVPVFALTT
310 320 330 340 350
GPRWPDVSGN SQATGTRAVT TGAPRPAWLD RCAAMNRHAI AAVREQLAAH
360 370 380 390 400
PLTTGLHVAA AVSHALRPGD QLVLGASNPV RDVALAGLDT RGIRVRSNRG
410 420 430 440 450
VAGIDGTVST AIGAALAYEG AHERTGSPDS PPRTIALIGD LTFVHDSSGL
460 470 480 490 500
LIGPTEPIPR SLTIVVSNDN GGGIFELLEQ GDPRFSDVSS RIFGTPHDVD
510 520 530 540 550
VGALCRAYHV ESRQIEVDEL GPTLDQPGAG MRVLEVKADR SSLRQLHAAI

KAAL
Length:554
Mass (Da):57,836
Last modified:October 1, 2003 - v1
Checksum:i879423AAB00F36A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIT99166.1
RefSeqiNP_854230.1, NC_002945.3
WP_003402927.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO41811; CDO41811; Mb0570
PATRICifig|233413.5.peg.618

Similar proteinsi

Entry informationi

Entry nameiMEND_MYCBO
AccessioniPrimary (citable) accession number: Q7U1S4
Secondary accession number(s): A0A1R3XVP4, X2BFE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: October 1, 2003
Last modified: April 25, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome