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Protein

Cyclopropane mycolic acid synthase MmaA2

Gene

cmaC

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathwayi: mycolic acid biosynthesis

This protein is involved in the pathway mycolic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway mycolic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Active sitei269By similarity1

GO - Molecular functioni

  • cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB-EC
  • methyltransferase activity Source: UniProtKB

GO - Biological processi

  • mycolic acid biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase MmaA2 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:cmaC
Synonyms:mmaA2, mmas-2
Ordered Locus Names:BQ2027_MB0663C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003983601 – 287Cyclopropane mycolic acid synthase MmaA2Add BLAST287

Structurei

3D structure databases

ProteinModelPortaliQ7U1J9.
SMRiQ7U1J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 34S-adenosyl-L-methionine bindingBy similarity2
Regioni72 – 74S-adenosyl-L-methionine bindingBy similarity3
Regioni94 – 99S-adenosyl-L-methionine bindingBy similarity6
Regioni123 – 124S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

HOGENOMiHOG000245191.
KOiK00574.
OMAiLHCITAQ.

Family and domain databases

InterProiView protein in InterPro
IPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7U1J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI
60 70 80 90 100
AKIDLALGKL GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA
110 120 130 140 150
AHVQKSFDEM DTPLDRRVLL AGWEQFNEPV DRIVSIGAFE HFGHDRHADF
160 170 180 190 200
FARAHKILPP DGVLLLHTIT GLTRQQMVDH GLPLTLWLAR FLKFIATEIF
210 220 230 240 250
PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA EALQEHKSEA
260 270 280
IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK
Length:287
Mass (Da):32,681
Last modified:October 1, 2003 - v1
Checksum:i4894FF920B090129
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208I → S in AAC44874 (PubMed:9044265).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77466 Genomic DNA. Translation: AAC44874.1.
LT708304 Genomic DNA. Translation: SIT99261.1.
RefSeqiNP_854321.1. NC_002945.3.
WP_003403304.1. NC_002945.4.

Genome annotation databases

EnsemblBacteriaiCDO41907; CDO41907; Mb0663c.
KEGGimbo:Mb0663c.
PATRICifig|233413.5.peg.723.

Similar proteinsi

Entry informationi

Entry nameiMMAA2_MYCBO
AccessioniPrimary (citable) accession number: Q7U1J9
Secondary accession number(s): A0A1R3XVZ3, P94923, X2BFN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2003
Last modified: October 25, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families