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Protein

Cyclopropane mycolic acid synthase MmaA2

Gene

cmaC

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Active sitei269 – 2691By similarity

GO - Molecular functioni

  1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB-EC
  2. methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mycolic acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase MmaA2 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:cmaC
Synonyms:mmaA2, mmas-2
Ordered Locus Names:Mb0663c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Cyclopropane mycolic acid synthase MmaA2PRO_0000398360Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb0663c.

Structurei

3D structure databases

ProteinModelPortaliQ7U1J9.
SMRiQ7U1J9. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 342S-adenosyl-L-methionine bindingBy similarity
Regioni72 – 743S-adenosyl-L-methionine bindingBy similarity
Regioni94 – 996S-adenosyl-L-methionine bindingBy similarity
Regioni123 – 1242S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiHPPTIEM.
OrthoDBiEOG6BGNZP.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7U1J9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI
60 70 80 90 100
AKIDLALGKL GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA
110 120 130 140 150
AHVQKSFDEM DTPLDRRVLL AGWEQFNEPV DRIVSIGAFE HFGHDRHADF
160 170 180 190 200
FARAHKILPP DGVLLLHTIT GLTRQQMVDH GLPLTLWLAR FLKFIATEIF
210 220 230 240 250
PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA EALQEHKSEA
260 270 280
IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK
Length:287
Mass (Da):32,681
Last modified:October 1, 2003 - v1
Checksum:i4894FF920B090129
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081I → S in AAC44874. (PubMed:9044265)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77466 Genomic DNA. Translation: AAC44874.1.
BX248333 Genomic DNA. Translation: CDO41907.1.
RefSeqiNP_854321.1. NC_002945.3.

Genome annotation databases

GeneIDi1091773.
KEGGimbo:Mb0663c.
PATRICi18003118. VBIMycBov88188_0723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77466 Genomic DNA. Translation: AAC44874.1.
BX248333 Genomic DNA. Translation: CDO41907.1.
RefSeqiNP_854321.1. NC_002945.3.

3D structure databases

ProteinModelPortaliQ7U1J9.
SMRiQ7U1J9. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233413.Mb0663c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1091773.
KEGGimbo:Mb0663c.
PATRICi18003118. VBIMycBov88188_0723.

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiHPPTIEM.
OrthoDBiEOG6BGNZP.

Enzyme and pathway databases

UniPathwayiUPA00915.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mycobacterium bovis BCG genes involved in the biosynthesis of cyclopropyl keto- and hydroxy-mycolic acids."
    Dubnau E., Laneelle M.-A., Soares S., Benichou A., Vaz T., Prome D., Prome J.-C., Daffe M., Quemard A.
    Mol. Microbiol. 23:313-322(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN OXYGEN-CONTAINING MYCOLATES BIOSYNTHESIS, NOMENCLATURE.
    Strain: BCG / Pasteur.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiMMAA2_MYCBO
AccessioniPrimary (citable) accession number: Q7U1J9
Secondary accession number(s): P94923, X2BFN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2003
Last modified: February 4, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.