Q7TYY6 (PKNL_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknL
EC=2.7.11.1

Gene names

Name:	pknL
Ordered Locus Names:	Mb2198

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Serine/threonine-protein kinase PknL		PRO_0000171225
Regions
	Topological domain	1 – 368	368	Cytoplasmic Potential
	Transmembrane	369 – 389	21	Helical; Potential
	Topological domain	390 – 399	10	Extracellular Potential
	Domain	19 – 278	260	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	142	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TYY6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E896CEA19FB5A16A	FASTA	399	42,818
	10 20 30 40 50 60 MVEAGTRDPL ESALLDSRYL VQAKIASGGT STVYRGLDVR LDRPVALKVM DARYAGDEQF 70 80 90 100 110 120 LTRFRLEARA VARLNNRALV AVYDQGKDGR HPFLVMELIE GGTLRELLIE RGPMPPHAVV 130 140 150 160 170 180 AVLRPVLGGL AAAHRAGLVH RDVKPENILI SDDGDVKLAD FGLVRAVAAA SITSTGVILG 190 200 210 220 230 240 TAAYLSPEQV RDGNADPRSD VYSVGVLVYE LLTGHTPFTG DSALSIAYQR LDADVPRASA 250 260 270 280 290 300 VIDGVPPQFD ELVACATARN PADRYADAIA MGADLEAIAE ELALPEFRVP APRNSAQHRS 310 320 330 340 350 360 AALYRSRITQ QGQLGAKPVH HPTRQLTRQP GDCSEPASGS EPEHEPITGQ FAGIAIEEFI 370 380 390 WARQHARRMV LVWVSVVLAI TGLVASAAWT IGSNLSGLL

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248341 Genomic DNA. Translation: CAD97051.1.
RefSeq	NP_855847.1. NC_002945.3.
3D structure databases
ProteinModelPortal	Q7TYY6.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb2198.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD97051; CAD97051; Mb2198.
GeneID	1091294.
KEGG	mbo:Mb2198.
PATRIC	18006536. VBIMycBov88188_2414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037187.
KO	K08884.
OMA	RTIVENI.
ProtClustDB	CLSK791676.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNL_MYCBO

Accession

Primary (citable) accession number: Q7TYY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program