Reviewed, UniProtKB/Swiss-Prot Q7TYP6 (SIR_MYCBO)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [ferredoxin]
    EC=1.8.7.1

Gene names

Name:	sir
Synonyms:	nirA
Ordered Locus Names:	Mb2412

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrogen sulfide + 6 oxidized ferredoxin + 3 H(2)O = sulfite + 6 reduced ferredoxin + 6 H(+).
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Thioether bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (ferredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Sulfite reductase [ferredoxin]		PRO_0000199949
Sites
	Metal binding	417	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	423	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	463	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	467	1	Iron (siroheme axial ligand) By similarity
	Metal binding	467	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Cross-link	69 ↔ 161		3'-(S-cysteinyl)-tyrosine (Tyr-Cys) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TYP6-1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 68925CA37C131BC3	FASTA	555	62,112
	10 20 30 40 50 60 MTTARPAKAR NEGQWALGHR EPLNANEELK KAGNPLDVRE RIENIYAKQG FDSIDKTDLR 70 80 90 100 110 120 GRFRWWGLYT QREQGYDGTW TGDDNIDKLE AKYFMMRVRC DGGALSAAAL RTLGQISTEF 130 140 150 160 170 180 ARDTADISDR QNVQYHWIEV ENVPEIWRRL DDVGLQTTEA CGDCPRVVLG SPLAGESLDE 190 200 210 220 230 240 VLDPTWAIEE IVRRYIGKPD FADLPRKYKT AISGLQDVAH EINDVAFIGV NHPEHGPGLD 250 260 270 280 290 300 LWVGGGLSTN PMLAQRVGAW VPLGEVPEVW AAVTSVFRDY GYRRLRAKAR LKFLIKDWGI 310 320 330 340 350 360 AKFREVLETE YLKRPLIDGP APEPVKHPID HVGVQRLKNG LNAVGVAPIA GRVSGTILTA 370 380 390 400 410 420 VADLMARAGS DRIRFTPYQK LVILDIPDAL LDDLIAGLDA LGLQSRPSHW RRNLMACSGI 430 440 450 460 470 480 EFCKLSFAET RVRAQHLVPE LERRLEDINS QLDVPITVNI NGCPNSCARI QIADIGFKGQ 490 500 510 520 530 540 MIDDGHGGSV EGFQVHLGGH LGLDAGFGRK LRQHKVTSDE LGDYIDRVVR NFVKHRSEGE 550 RFAQWVIRAE EDDLR

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248342 Genomic DNA. Translation: CAD97273.1. Different initiation.
RefSeq	NP_856061.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1094301.
GenomeReviews	Gene locus Mb2412 in contig BX248333_GR.
KEGG	mbo:Mb2412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7TYP6.
Family and domain databases
InterPro	IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIR_MYCBO

Accession

Primary (citable) accession number: Q7TYP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	November 25, 2008
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)