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Q7TXM0

- PPSA_MYCBO

UniProt

Q7TXM0 - PPSA_MYCBO

Protein

Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA

Gene

ppsA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

    Cofactori

    Binds 2 phosphopantetheines covalently.By similarity
    NADP.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei273 – 2731For beta-ketoacyl synthase activityPROSITE-ProRule annotation
    Active sitei720 – 7201For malonyltransferase activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1492 – 155160NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
    2. oxidoreductase activity Source: UniProtKB-KW
    3. phosphopantetheine binding Source: InterPro

    GO - Biological processi

    1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
    2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
    3. phenolic phthiocerol biosynthetic process Source: UniProtKB
    4. phthiocerol biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17242.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA
    Alternative name(s):
    Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
    Gene namesi
    Name:ppsA
    Ordered Locus Names:Mb2956
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. polyketide synthase complex Source: UniProtKB

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18761876Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsAPRO_0000406945Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
    Modified residuei1796 – 17961O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb2956.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TXM0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 8074Acyl carrier 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1764 – 183370Acyl carrier 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni104 – 529426Beta-ketoacyl synthaseBy similarityAdd
    BLAST
    Regioni624 – 950327AcyltransferaseBy similarityAdd
    BLAST
    Regioni1491 – 1728238Beta-ketoacyl reductaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 2 acyl carrier domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG3321.
    HOGENOMiHOG000046292.
    KOiK12440.
    OMAiRIIASEH.
    OrthoDBiEOG6W19KW.

    Family and domain databases

    Gene3Di1.10.1200.10. 2 hits.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 2 hits.
    [Graphical view]
    SMARTiSM00827. PKS_AT. 1 hit.
    SM00822. PKS_KR. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 2 hits.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TXM0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGSISGEAD LRHWLIDYLV TNIGCTPDEV DPDLSLADLG VSSRDAVVLS     50
    GELSELLGRT VSPIDFWEHP TINALAAYLA APEPSPDSDA AVKRGARNSL 100
    DEPIAVVGMG CRFPGGISCP EALWDFLCER RSSISQVPPQ RWQPFEGGPP 150
    EVAAALARTT RWGSFLPDID AFDAEFFEIS PSEADKMDPQ QRLLLEVAWE 200
    ALEHAGIPPG TLRRSATGVF AGACLSEYGA MASADLSQVD GWSNSGGAMS 250
    IIANRLSYFL DLRGPSVAVD TACSSSLVAI HLACQSLRTQ DCHLAIAAGV 300
    NLLLSPAVFR GFDQVGALSP TGQCRAFDAT ADGFVRGEGA GVVVLKRLTD 350
    AQRDGDRVLA VICGSAVTQD GRSNGLMAPN PAAQMAVLRA AYTNAGMQPS 400
    EVDYVEAHGT GTLLGDPIEA RALGTVLGRG RPEDSPLLIG SVKTNLGHTE 450
    AAAGIAGFIK TVLAVQHGQI PPNQHFETAN PHIPFTDLRM KVVDTQTEWP 500
    ATGHPRRAGV SSFGFGGTNA HVVIEQGQEV RPAPGQGLSP AVSTLVVAGK 550
    TMQRVSATAG MLADWMEGPG ADVALADVAH TLNHHRSRQP KFGTVVARDR 600
    TQAIAGLRAL AAGQHAPGVV NPAEGSPGPG TVFVYSGRGS QWAGMGRQLL 650
    ADEPAFAAAV AELEPVFVEQ AGFSLHDVLA NGEELVGIEQ IQLGLIGMQL 700
    ALTELWCSYG VQPDLVIGHS MGEVAAAVVA GALTPAEGLR VTATRSRLMA 750
    PLSGQGGMAL LELDAPTTEA LIADFPQVTL GIYNSPRQTV IAGPTEQIDE 800
    LITRVRARDR FASRVNIEVA PHNPAMDALQ PAMRSELADL TPRTPTIGII 850
    STTYADLHTQ PVFDAEHWAT NMRNPVHFQQ AIASAGSGAD GAYHTFIEIS 900
    AHPLLTQAII DTLHSAQPGA RYTSLGTLQR DTDDVVTFRT NLNKAHTIHP 950
    PHTPHPPEPH PPIPTTPWQH TRHWITTKYP AGSVGSAPRA GTLLGQHTTV 1000
    ATVSASPPSH LWQARLAPDA KPYQGGHRFH QVEVVPASVV LHTILSAATE 1050
    LGYSALSEVR FEQPIFADRP RLIQVVADNR AISLASSPAA GTPSDRWTRH 1100
    VTAQLSSSPS DSASSLNEHH RANGQPPERA HRDLIPDLAE LLAMRGIDGL 1150
    PFSWTVASWT QHSSNLTVAI DLPEALPEGS TGPLLDAAVH LAALSDVADS 1200
    RLYVPASIEQ ISLGDVVTGP RSSVTLNRTA HDDDGITVDV TVAAHGEVPS 1250
    LSMRSLRYRA LDFGLDVGRA QPPASTGPVE AYCDATNFVH TIDWQPQTVP 1300
    DATHPGAEQV THPGPVAIIG DDGAALCETL EGAGYQPAVM SDGVSQARYV 1350
    VYVADSDPAG ADETDVDFAV RICTEITGLV RTLAERDADK PAALWILTRG 1400
    VHESVAPSAL RQSFLWGLAG VIAAEHPELW GGLVDLAIND DLGEFGPALA 1450
    ELLAKPSKSI LVRRDGVVLA PALAPVRGEP ARKSLQCRPD AAYLITGGLG 1500
    ALGLLMADWL ADRGAHRLVL TGRTPLPPRR DWQLDTLDTE LRRRIDAIRA 1550
    LEMRGVTVEA VAADVGCRED VQALLAARDR DGAAPIRGII HAAGITNDQL 1600
    VTSMTGDAVR QVMWPKIGGS QVLHDAFPPG SVDFFYLTAS AAGIFGIPGQ 1650
    GSYAAANSYL DALARARRQQ GCHTMSLDWV AWRGLGLAAD AQLVSEELAR 1700
    MGSRDITPSE AFTAWEFVDG YDVAQAVVVP MPAPAGADGS GANAYLLPAR 1750
    NWSVMAATEV RSELEQGLRR IIAAELRVPE KELDTDRPFA ELGLNSLMAM 1800
    AIRREAEQFV GIELSATMLF NHPTVKSLAS YLAKRVAPHD VSQDNQISAL 1850
    SSSAGSVLDS LFDRIESAPP EAERSV 1876
    Length:1,876
    Mass (Da):198,848
    Last modified:October 1, 2003 - v1
    Checksum:iD44006C79AEF6AAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44223.1.
    RefSeqiNP_856601.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD96643; CAD96643; Mb2956.
    GeneIDi1092150.
    KEGGimbo:Mb2956.
    PATRICi18008224. VBIMycBov88188_3244.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44223.1 .
    RefSeqi NP_856601.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali Q7TXM0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb2956.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD96643 ; CAD96643 ; Mb2956 .
    GeneIDi 1092150.
    KEGGi mbo:Mb2956.
    PATRICi 18008224. VBIMycBov88188_3244.

    Phylogenomic databases

    eggNOGi COG3321.
    HOGENOMi HOG000046292.
    KOi K12440.
    OMAi RIIASEH.
    OrthoDBi EOG6W19KW.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci MetaCyc:MONOMER-17242.

    Family and domain databases

    Gene3Di 1.10.1200.10. 2 hits.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 2 hits.
    [Graphical view ]
    SMARTi SM00827. PKS_AT. 1 hit.
    SM00822. PKS_KR. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.
    2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
      Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
      J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiPPSA_MYCBO
    AccessioniPrimary (citable) accession number: Q7TXM0
    Secondary accession number(s): X2BM73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3