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Q7TXM0

- PPSA_MYCBO

UniProt

Q7TXM0 - PPSA_MYCBO

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Protein
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA
Gene
ppsA, Mb2956
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Cofactori

Binds 2 phosphopantetheines covalently By similarity.
NADP By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731For beta-ketoacyl synthase activity By similarity
Active sitei720 – 7201For malonyltransferase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1492 – 155160NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
  3. phosphopantetheine binding Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. phenolic phthiocerol biosynthetic process Source: UniProtKB
  4. phthiocerol biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17242.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsA
Ordered Locus Names:Mb2956
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18761876Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA
PRO_0000406945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431O-(pantetheine 4'-phosphoryl)serine By similarity
Modified residuei1796 – 17961O-(pantetheine 4'-phosphoryl)serine By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2956.

Structurei

3D structure databases

ProteinModelPortaliQ7TXM0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 8074Acyl carrier 1
Add
BLAST
Domaini1764 – 183370Acyl carrier 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 529426Beta-ketoacyl synthase By similarity
Add
BLAST
Regioni624 – 950327Acyltransferase By similarity
Add
BLAST
Regioni1491 – 1728238Beta-ketoacyl reductase By similarity
Add
BLAST

Sequence similaritiesi

Contains 2 acyl carrier domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3321.
HOGENOMiHOG000046292.
KOiK12440.
OMAiRIIASEH.
OrthoDBiEOG6W19KW.

Family and domain databases

Gene3Di1.10.1200.10. 2 hits.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 2 hits.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 2 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXM0-1 [UniParc]FASTAAdd to Basket

« Hide

MTGSISGEAD LRHWLIDYLV TNIGCTPDEV DPDLSLADLG VSSRDAVVLS     50
GELSELLGRT VSPIDFWEHP TINALAAYLA APEPSPDSDA AVKRGARNSL 100
DEPIAVVGMG CRFPGGISCP EALWDFLCER RSSISQVPPQ RWQPFEGGPP 150
EVAAALARTT RWGSFLPDID AFDAEFFEIS PSEADKMDPQ QRLLLEVAWE 200
ALEHAGIPPG TLRRSATGVF AGACLSEYGA MASADLSQVD GWSNSGGAMS 250
IIANRLSYFL DLRGPSVAVD TACSSSLVAI HLACQSLRTQ DCHLAIAAGV 300
NLLLSPAVFR GFDQVGALSP TGQCRAFDAT ADGFVRGEGA GVVVLKRLTD 350
AQRDGDRVLA VICGSAVTQD GRSNGLMAPN PAAQMAVLRA AYTNAGMQPS 400
EVDYVEAHGT GTLLGDPIEA RALGTVLGRG RPEDSPLLIG SVKTNLGHTE 450
AAAGIAGFIK TVLAVQHGQI PPNQHFETAN PHIPFTDLRM KVVDTQTEWP 500
ATGHPRRAGV SSFGFGGTNA HVVIEQGQEV RPAPGQGLSP AVSTLVVAGK 550
TMQRVSATAG MLADWMEGPG ADVALADVAH TLNHHRSRQP KFGTVVARDR 600
TQAIAGLRAL AAGQHAPGVV NPAEGSPGPG TVFVYSGRGS QWAGMGRQLL 650
ADEPAFAAAV AELEPVFVEQ AGFSLHDVLA NGEELVGIEQ IQLGLIGMQL 700
ALTELWCSYG VQPDLVIGHS MGEVAAAVVA GALTPAEGLR VTATRSRLMA 750
PLSGQGGMAL LELDAPTTEA LIADFPQVTL GIYNSPRQTV IAGPTEQIDE 800
LITRVRARDR FASRVNIEVA PHNPAMDALQ PAMRSELADL TPRTPTIGII 850
STTYADLHTQ PVFDAEHWAT NMRNPVHFQQ AIASAGSGAD GAYHTFIEIS 900
AHPLLTQAII DTLHSAQPGA RYTSLGTLQR DTDDVVTFRT NLNKAHTIHP 950
PHTPHPPEPH PPIPTTPWQH TRHWITTKYP AGSVGSAPRA GTLLGQHTTV 1000
ATVSASPPSH LWQARLAPDA KPYQGGHRFH QVEVVPASVV LHTILSAATE 1050
LGYSALSEVR FEQPIFADRP RLIQVVADNR AISLASSPAA GTPSDRWTRH 1100
VTAQLSSSPS DSASSLNEHH RANGQPPERA HRDLIPDLAE LLAMRGIDGL 1150
PFSWTVASWT QHSSNLTVAI DLPEALPEGS TGPLLDAAVH LAALSDVADS 1200
RLYVPASIEQ ISLGDVVTGP RSSVTLNRTA HDDDGITVDV TVAAHGEVPS 1250
LSMRSLRYRA LDFGLDVGRA QPPASTGPVE AYCDATNFVH TIDWQPQTVP 1300
DATHPGAEQV THPGPVAIIG DDGAALCETL EGAGYQPAVM SDGVSQARYV 1350
VYVADSDPAG ADETDVDFAV RICTEITGLV RTLAERDADK PAALWILTRG 1400
VHESVAPSAL RQSFLWGLAG VIAAEHPELW GGLVDLAIND DLGEFGPALA 1450
ELLAKPSKSI LVRRDGVVLA PALAPVRGEP ARKSLQCRPD AAYLITGGLG 1500
ALGLLMADWL ADRGAHRLVL TGRTPLPPRR DWQLDTLDTE LRRRIDAIRA 1550
LEMRGVTVEA VAADVGCRED VQALLAARDR DGAAPIRGII HAAGITNDQL 1600
VTSMTGDAVR QVMWPKIGGS QVLHDAFPPG SVDFFYLTAS AAGIFGIPGQ 1650
GSYAAANSYL DALARARRQQ GCHTMSLDWV AWRGLGLAAD AQLVSEELAR 1700
MGSRDITPSE AFTAWEFVDG YDVAQAVVVP MPAPAGADGS GANAYLLPAR 1750
NWSVMAATEV RSELEQGLRR IIAAELRVPE KELDTDRPFA ELGLNSLMAM 1800
AIRREAEQFV GIELSATMLF NHPTVKSLAS YLAKRVAPHD VSQDNQISAL 1850
SSSAGSVLDS LFDRIESAPP EAERSV 1876
Length:1,876
Mass (Da):198,848
Last modified:October 1, 2003 - v1
Checksum:iD44006C79AEF6AAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44223.1.
RefSeqiNP_856601.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96643; CAD96643; Mb2956.
GeneIDi1092150.
KEGGimbo:Mb2956.
PATRICi18008224. VBIMycBov88188_3244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44223.1 .
RefSeqi NP_856601.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXM0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2956.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96643 ; CAD96643 ; Mb2956 .
GeneIDi 1092150.
KEGGi mbo:Mb2956.
PATRICi 18008224. VBIMycBov88188_3244.

Phylogenomic databases

eggNOGi COG3321.
HOGENOMi HOG000046292.
KOi K12440.
OMAi RIIASEH.
OrthoDBi EOG6W19KW.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci MetaCyc:MONOMER-17242.

Family and domain databases

Gene3Di 1.10.1200.10. 2 hits.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 2 hits.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 2 hits.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
    Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
    J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPPSA_MYCBO
AccessioniPrimary (citable) accession number: Q7TXM0
Secondary accession number(s): X2BM73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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