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Q7TXM0

- PPSA_MYCBO

UniProt

Q7TXM0 - PPSA_MYCBO

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Protein

Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA

Gene

ppsA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

Cofactori

Binds 2 phosphopantetheines covalently.By similarity
NADP.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731For beta-ketoacyl synthase activityPROSITE-ProRule annotation
Active sitei720 – 7201For malonyltransferase activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1492 – 155160NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
  3. phosphopantetheine binding Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. phenolic phthiocerol biosynthetic process Source: UniProtKB
  4. phthiocerol biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17242.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsA
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsA
Ordered Locus Names:Mb2956
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18761876Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsAPRO_0000406945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
Modified residuei1796 – 17961O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2956.

Structurei

3D structure databases

ProteinModelPortaliQ7TXM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 8074Acyl carrier 1PROSITE-ProRule annotationAdd
BLAST
Domaini1764 – 183370Acyl carrier 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 529426Beta-ketoacyl synthaseBy similarityAdd
BLAST
Regioni624 – 950327AcyltransferaseBy similarityAdd
BLAST
Regioni1491 – 1728238Beta-ketoacyl reductaseBy similarityAdd
BLAST

Sequence similaritiesi

Contains 2 acyl carrier domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3321.
HOGENOMiHOG000046292.
KOiK12440.
OMAiRIIASEH.
OrthoDBiEOG6W19KW.

Family and domain databases

Gene3Di1.10.1200.10. 2 hits.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 2 hits.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 2 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXM0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGSISGEAD LRHWLIDYLV TNIGCTPDEV DPDLSLADLG VSSRDAVVLS
60 70 80 90 100
GELSELLGRT VSPIDFWEHP TINALAAYLA APEPSPDSDA AVKRGARNSL
110 120 130 140 150
DEPIAVVGMG CRFPGGISCP EALWDFLCER RSSISQVPPQ RWQPFEGGPP
160 170 180 190 200
EVAAALARTT RWGSFLPDID AFDAEFFEIS PSEADKMDPQ QRLLLEVAWE
210 220 230 240 250
ALEHAGIPPG TLRRSATGVF AGACLSEYGA MASADLSQVD GWSNSGGAMS
260 270 280 290 300
IIANRLSYFL DLRGPSVAVD TACSSSLVAI HLACQSLRTQ DCHLAIAAGV
310 320 330 340 350
NLLLSPAVFR GFDQVGALSP TGQCRAFDAT ADGFVRGEGA GVVVLKRLTD
360 370 380 390 400
AQRDGDRVLA VICGSAVTQD GRSNGLMAPN PAAQMAVLRA AYTNAGMQPS
410 420 430 440 450
EVDYVEAHGT GTLLGDPIEA RALGTVLGRG RPEDSPLLIG SVKTNLGHTE
460 470 480 490 500
AAAGIAGFIK TVLAVQHGQI PPNQHFETAN PHIPFTDLRM KVVDTQTEWP
510 520 530 540 550
ATGHPRRAGV SSFGFGGTNA HVVIEQGQEV RPAPGQGLSP AVSTLVVAGK
560 570 580 590 600
TMQRVSATAG MLADWMEGPG ADVALADVAH TLNHHRSRQP KFGTVVARDR
610 620 630 640 650
TQAIAGLRAL AAGQHAPGVV NPAEGSPGPG TVFVYSGRGS QWAGMGRQLL
660 670 680 690 700
ADEPAFAAAV AELEPVFVEQ AGFSLHDVLA NGEELVGIEQ IQLGLIGMQL
710 720 730 740 750
ALTELWCSYG VQPDLVIGHS MGEVAAAVVA GALTPAEGLR VTATRSRLMA
760 770 780 790 800
PLSGQGGMAL LELDAPTTEA LIADFPQVTL GIYNSPRQTV IAGPTEQIDE
810 820 830 840 850
LITRVRARDR FASRVNIEVA PHNPAMDALQ PAMRSELADL TPRTPTIGII
860 870 880 890 900
STTYADLHTQ PVFDAEHWAT NMRNPVHFQQ AIASAGSGAD GAYHTFIEIS
910 920 930 940 950
AHPLLTQAII DTLHSAQPGA RYTSLGTLQR DTDDVVTFRT NLNKAHTIHP
960 970 980 990 1000
PHTPHPPEPH PPIPTTPWQH TRHWITTKYP AGSVGSAPRA GTLLGQHTTV
1010 1020 1030 1040 1050
ATVSASPPSH LWQARLAPDA KPYQGGHRFH QVEVVPASVV LHTILSAATE
1060 1070 1080 1090 1100
LGYSALSEVR FEQPIFADRP RLIQVVADNR AISLASSPAA GTPSDRWTRH
1110 1120 1130 1140 1150
VTAQLSSSPS DSASSLNEHH RANGQPPERA HRDLIPDLAE LLAMRGIDGL
1160 1170 1180 1190 1200
PFSWTVASWT QHSSNLTVAI DLPEALPEGS TGPLLDAAVH LAALSDVADS
1210 1220 1230 1240 1250
RLYVPASIEQ ISLGDVVTGP RSSVTLNRTA HDDDGITVDV TVAAHGEVPS
1260 1270 1280 1290 1300
LSMRSLRYRA LDFGLDVGRA QPPASTGPVE AYCDATNFVH TIDWQPQTVP
1310 1320 1330 1340 1350
DATHPGAEQV THPGPVAIIG DDGAALCETL EGAGYQPAVM SDGVSQARYV
1360 1370 1380 1390 1400
VYVADSDPAG ADETDVDFAV RICTEITGLV RTLAERDADK PAALWILTRG
1410 1420 1430 1440 1450
VHESVAPSAL RQSFLWGLAG VIAAEHPELW GGLVDLAIND DLGEFGPALA
1460 1470 1480 1490 1500
ELLAKPSKSI LVRRDGVVLA PALAPVRGEP ARKSLQCRPD AAYLITGGLG
1510 1520 1530 1540 1550
ALGLLMADWL ADRGAHRLVL TGRTPLPPRR DWQLDTLDTE LRRRIDAIRA
1560 1570 1580 1590 1600
LEMRGVTVEA VAADVGCRED VQALLAARDR DGAAPIRGII HAAGITNDQL
1610 1620 1630 1640 1650
VTSMTGDAVR QVMWPKIGGS QVLHDAFPPG SVDFFYLTAS AAGIFGIPGQ
1660 1670 1680 1690 1700
GSYAAANSYL DALARARRQQ GCHTMSLDWV AWRGLGLAAD AQLVSEELAR
1710 1720 1730 1740 1750
MGSRDITPSE AFTAWEFVDG YDVAQAVVVP MPAPAGADGS GANAYLLPAR
1760 1770 1780 1790 1800
NWSVMAATEV RSELEQGLRR IIAAELRVPE KELDTDRPFA ELGLNSLMAM
1810 1820 1830 1840 1850
AIRREAEQFV GIELSATMLF NHPTVKSLAS YLAKRVAPHD VSQDNQISAL
1860 1870
SSSAGSVLDS LFDRIESAPP EAERSV
Length:1,876
Mass (Da):198,848
Last modified:October 1, 2003 - v1
Checksum:iD44006C79AEF6AAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44223.1.
RefSeqiNP_856601.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96643; CAD96643; Mb2956.
GeneIDi1092150.
KEGGimbo:Mb2956.
PATRICi18008224. VBIMycBov88188_3244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44223.1 .
RefSeqi NP_856601.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXM0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2956.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96643 ; CAD96643 ; Mb2956 .
GeneIDi 1092150.
KEGGi mbo:Mb2956.
PATRICi 18008224. VBIMycBov88188_3244.

Phylogenomic databases

eggNOGi COG3321.
HOGENOMi HOG000046292.
KOi K12440.
OMAi RIIASEH.
OrthoDBi EOG6W19KW.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci MetaCyc:MONOMER-17242.

Family and domain databases

Gene3Di 1.10.1200.10. 2 hits.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 2 hits.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 2 hits.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
    Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
    J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPPSA_MYCBO
AccessioniPrimary (citable) accession number: Q7TXM0
Secondary accession number(s): X2BM73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: October 1, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3