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Q7TXL9 (PPSB_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I
EC=2.3.1.41
Gene names
Name:ppsB
Ordered Locus Names:Mb2957
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length1538 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively. Ref.2

Catalytic activity

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Cofactor

Binds 1 phosphopantetheines covalently By similarity.

NADP By similarity.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Disruption phenotype

Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope. Ref.2

Sequence similarities

Contains 1 acyl carrier domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15381538Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
PRO_0000406946

Regions

Domain1409 – 149587Acyl carrier
Nucleotide binding1153 – 119644NADP By similarity
Region36 – 458423Beta-ketoacyl synthase By similarity
Region553 – 882330Acyltransferase By similarity
Region1153 – 1328176Beta-ketoacyl reductase By similarity

Sites

Active site2051For beta-ketoacyl synthase activity By similarity
Active site6491For malonyltransferase activity By similarity

Amino acid modifications

Modified residue14581O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7TXL9 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: BD08742044AAFECC

FASTA1,538162,485
        10         20         30         40         50         60 
MMRTAFSRIS GMTAQQRTSL ADEFDRVSRI AVAEPVAVVG IGCRFPGDVD GPESFWDFLV 

        70         80         90        100        110        120 
AGRNAISTVP ADRWDAEAFY HPDPLTPGRM TTKWGGFVPD VAGFDAEFFG ITPREAAAMD 

       130        140        150        160        170        180 
PQQRMLLEVA WEALEHAGIP PDSLGGTRTA VMMGVYFNEY QSMLAASPQN VDAYSGTGNA 

       190        200        210        220        230        240 
HSITVGRISY LLGLRGPAVA VDTACSSSLV AVHLACQSLR LRETDLALAG GVSITLRPET 

       250        260        270        280        290        300 
QIAISAWGLL SPQGRCAAFD AAADGFVRGE GAGVVVLKRL TDAVRDGDQV LAVVRGSAVN 

       310        320        330        340        350        360 
QDGRSNGVTA PNTAAQCDVI ADALRSGDVA PDSVNYVEAH GTGTVLGDPI EFEALAATYG 

       370        380        390        400        410        420 
HGGDACALGA VKTNIGHLEA AAGIAGFIKA TLAVQRATIP PNLHFSQWNP AIDAASTRFF 

       430        440        450        460        470        480 
VPTQNSPWPT AEGPRRAAVS SFGLGGTNAH VIIEQGSELA PVSEGGEDTG VSTLVVTGKT 

       490        500        510        520        530        540 
AQRMAATAQV LADWMEGPGA EVAVADVAHT VNHHRARQAT FGTVVARDRA QAIAGLRALA 

       550        560        570        580        590        600 
AGQHAPGVVS HQDGSPGPGT VFVYSGRGSQ WAGMGRQLLA DEPAFAAAVA ELEPVFVEQA 

       610        620        630        640        650        660 
GFSLRDVIAT GKELVGIEQI QLGLIGMQLT LTELWRSYGV QPDLVIGHSM GEVAAAVVAG 

       670        680        690        700        710        720 
ALTPAEGLRV TATRARLMAP LSGQGGMALL GLDAAATEAL IADYPQVTVG IYNSPRQTVI 

       730        740        750        760        770        780 
AGPTEQIDEL IARVRAQNRF ASRVNIEVAP HNPAMDALQP AMRSELADLT PRTPTIGIIS 

       790        800        810        820        830        840 
TTYADLHTQP IFDAEHWATN MRNPVRFQQA IASAGSGADG AYHTFIEISA HPLLTQAIAD 

       850        860        870        880        890        900 
TLEDAHRPTK SAAKYLSIGT LQRDADDTVT FRTNLYTADI AHPPHTCHPP EPHPTIPTTP 

       910        920        930        940        950        960 
WQHTHHWIAT THPSTAAPED PGSNKVVVNG QSTSESRALE DWCHQLAWPI RPAVSADPPS 

       970        980        990       1000       1010       1020 
TAAWLVVADN ELCHELARAA DSRVDSLSPP ALAAGSDPAA LLDALRGVDN VLYAPPVPGE 

      1030       1040       1050       1060       1070       1080 
LLDIESAYQV FHATRRLAAA MVASSATAIS PPKLFIMTRN AQPISEGDRA NPGHAVLWGL 

      1090       1100       1110       1120       1130       1140 
GRSLALEHPE IWGGIIDLDD SMPAELAVRH VLTAAHGTDG EDQVVYRSGA RHVPRLQRRT 

      1150       1160       1170       1180       1190       1200 
LPGKPVTLNA DASQLVIGAT GNIGPHLIRQ LARMGAKTIV AMARKPGALD ELTQCLAATG 

      1210       1220       1230       1240       1250       1260 
TDLIAVAADA TDPAAMQTLF DRFGTELPPL EGIYLAAFAG RPALLSEMTD DDVTTMFRPK 

      1270       1280       1290       1300       1310       1320 
LDALALLHRL SLKSPVRHFV LFSSVSGLLG SRWLAHYTAT SAFLDSFAGA RRTMGLPATV 

      1330       1340       1350       1360       1370       1380 
VDWGLWKSLA DVQKDATQIS AESGLQPMAD EVAIGALPLV MNPDAAVATV VVAADWPLLA 

      1390       1400       1410       1420       1430       1440 
AAYRTRGALR IVDDLLPAPE DVGKGESEFR TSLRSCPAEK RRDMLFDHVG ALAATVMGMP 

      1450       1460       1470       1480       1490       1500 
PTEPLDPSAG FFQLGMDSLM SVTLQRALSE SLGEFLPASV VFDYPTVYSL TDYLATVLPE 

      1510       1520       1530 
LLEIGATAVA TQQATDSYHE LTEAELLEQL SERLRGTQ 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Mycobacterium bovis."
Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. expand/collapse author list , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-935 / AF2122/97.
[2]"Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248344 Genomic DNA. Translation: CAD96644.1.
RefSeqNP_856602.1. NC_002945.3.

3D structure databases

ProteinModelPortalQ7TXL9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb2957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD96644; CAD96644; Mb2957.
GeneID1092149.
KEGGmbo:Mb2957.
PATRIC18008226. VBIMycBov88188_3245.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3321.
HOGENOMHOG000046292.
KOK12441.
OMAVAMFRPK.
OrthoDBEOG6QP0WP.
ProtClustDBCLSK792170.

Enzyme and pathway databases

UniPathwayUPA00094.

Family and domain databases

Gene3D1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTSM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPSB_MYCBO
AccessionPrimary (citable) accession number: Q7TXL9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways