Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7TXL9

- PPSB_MYCBO

UniProt

Q7TXL9 - PPSB_MYCBO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB

Gene

ppsB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051For beta-ketoacyl synthase activityPROSITE-ProRule annotation
Active sitei649 – 6491For malonyltransferase activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1153 – 119644NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
  3. phosphopantetheine binding Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. phenolic phthiocerol biosynthetic process Source: UniProtKB
  4. phthiocerol biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsB
Ordered Locus Names:Mb2957
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15381538Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsBPRO_0000406946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1458 – 14581O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2957.

Structurei

3D structure databases

ProteinModelPortaliQ7TXL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1409 – 149587Acyl carrierPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 458423Beta-ketoacyl synthaseBy similarityAdd
BLAST
Regioni553 – 882330AcyltransferaseBy similarityAdd
BLAST
Regioni1153 – 1328176Beta-ketoacyl reductaseBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3321.
HOGENOMiHOG000046292.
KOiK12441.
OMAiVAMFRPK.
OrthoDBiEOG6QP0WP.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXL9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMRTAFSRIS GMTAQQRTSL ADEFDRVSRI AVAEPVAVVG IGCRFPGDVD
60 70 80 90 100
GPESFWDFLV AGRNAISTVP ADRWDAEAFY HPDPLTPGRM TTKWGGFVPD
110 120 130 140 150
VAGFDAEFFG ITPREAAAMD PQQRMLLEVA WEALEHAGIP PDSLGGTRTA
160 170 180 190 200
VMMGVYFNEY QSMLAASPQN VDAYSGTGNA HSITVGRISY LLGLRGPAVA
210 220 230 240 250
VDTACSSSLV AVHLACQSLR LRETDLALAG GVSITLRPET QIAISAWGLL
260 270 280 290 300
SPQGRCAAFD AAADGFVRGE GAGVVVLKRL TDAVRDGDQV LAVVRGSAVN
310 320 330 340 350
QDGRSNGVTA PNTAAQCDVI ADALRSGDVA PDSVNYVEAH GTGTVLGDPI
360 370 380 390 400
EFEALAATYG HGGDACALGA VKTNIGHLEA AAGIAGFIKA TLAVQRATIP
410 420 430 440 450
PNLHFSQWNP AIDAASTRFF VPTQNSPWPT AEGPRRAAVS SFGLGGTNAH
460 470 480 490 500
VIIEQGSELA PVSEGGEDTG VSTLVVTGKT AQRMAATAQV LADWMEGPGA
510 520 530 540 550
EVAVADVAHT VNHHRARQAT FGTVVARDRA QAIAGLRALA AGQHAPGVVS
560 570 580 590 600
HQDGSPGPGT VFVYSGRGSQ WAGMGRQLLA DEPAFAAAVA ELEPVFVEQA
610 620 630 640 650
GFSLRDVIAT GKELVGIEQI QLGLIGMQLT LTELWRSYGV QPDLVIGHSM
660 670 680 690 700
GEVAAAVVAG ALTPAEGLRV TATRARLMAP LSGQGGMALL GLDAAATEAL
710 720 730 740 750
IADYPQVTVG IYNSPRQTVI AGPTEQIDEL IARVRAQNRF ASRVNIEVAP
760 770 780 790 800
HNPAMDALQP AMRSELADLT PRTPTIGIIS TTYADLHTQP IFDAEHWATN
810 820 830 840 850
MRNPVRFQQA IASAGSGADG AYHTFIEISA HPLLTQAIAD TLEDAHRPTK
860 870 880 890 900
SAAKYLSIGT LQRDADDTVT FRTNLYTADI AHPPHTCHPP EPHPTIPTTP
910 920 930 940 950
WQHTHHWIAT THPSTAAPED PGSNKVVVNG QSTSESRALE DWCHQLAWPI
960 970 980 990 1000
RPAVSADPPS TAAWLVVADN ELCHELARAA DSRVDSLSPP ALAAGSDPAA
1010 1020 1030 1040 1050
LLDALRGVDN VLYAPPVPGE LLDIESAYQV FHATRRLAAA MVASSATAIS
1060 1070 1080 1090 1100
PPKLFIMTRN AQPISEGDRA NPGHAVLWGL GRSLALEHPE IWGGIIDLDD
1110 1120 1130 1140 1150
SMPAELAVRH VLTAAHGTDG EDQVVYRSGA RHVPRLQRRT LPGKPVTLNA
1160 1170 1180 1190 1200
DASQLVIGAT GNIGPHLIRQ LARMGAKTIV AMARKPGALD ELTQCLAATG
1210 1220 1230 1240 1250
TDLIAVAADA TDPAAMQTLF DRFGTELPPL EGIYLAAFAG RPALLSEMTD
1260 1270 1280 1290 1300
DDVTTMFRPK LDALALLHRL SLKSPVRHFV LFSSVSGLLG SRWLAHYTAT
1310 1320 1330 1340 1350
SAFLDSFAGA RRTMGLPATV VDWGLWKSLA DVQKDATQIS AESGLQPMAD
1360 1370 1380 1390 1400
EVAIGALPLV MNPDAAVATV VVAADWPLLA AAYRTRGALR IVDDLLPAPE
1410 1420 1430 1440 1450
DVGKGESEFR TSLRSCPAEK RRDMLFDHVG ALAATVMGMP PTEPLDPSAG
1460 1470 1480 1490 1500
FFQLGMDSLM SVTLQRALSE SLGEFLPASV VFDYPTVYSL TDYLATVLPE
1510 1520 1530
LLEIGATAVA TQQATDSYHE LTEAELLEQL SERLRGTQ
Length:1,538
Mass (Da):162,485
Last modified:October 1, 2003 - v1
Checksum:iBD08742044AAFECC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44224.1.
RefSeqiNP_856602.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96644; CAD96644; Mb2957.
GeneIDi1092149.
KEGGimbo:Mb2957.
PATRICi18008226. VBIMycBov88188_3245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44224.1 .
RefSeqi NP_856602.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXL9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96644 ; CAD96644 ; Mb2957 .
GeneIDi 1092149.
KEGGi mbo:Mb2957.
PATRICi 18008226. VBIMycBov88188_3245.

Phylogenomic databases

eggNOGi COG3321.
HOGENOMi HOG000046292.
KOi K12441.
OMAi VAMFRPK.
OrthoDBi EOG6QP0WP.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
    Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
    J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPPSB_MYCBO
AccessioniPrimary (citable) accession number: Q7TXL9
Secondary accession number(s): X2BM25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3