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Q7TXL9

- PPSB_MYCBO

UniProt

Q7TXL9 - PPSB_MYCBO

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Protein
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
Gene
ppsB, Mb2957
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Cofactori

Binds 1 phosphopantetheines covalently By similarity.
NADP By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051For beta-ketoacyl synthase activity By similarity
Active sitei649 – 6491For malonyltransferase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1153 – 119644NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
  3. phosphopantetheine binding Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. phenolic phthiocerol biosynthetic process Source: UniProtKB
  4. phthiocerol biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsB
Ordered Locus Names:Mb2957
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15381538Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
PRO_0000406946Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1458 – 14581O-(pantetheine 4'-phosphoryl)serine By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2957.

Structurei

3D structure databases

ProteinModelPortaliQ7TXL9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1409 – 149587Acyl carrier
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 458423Beta-ketoacyl synthase By similarity
Add
BLAST
Regioni553 – 882330Acyltransferase By similarity
Add
BLAST
Regioni1153 – 1328176Beta-ketoacyl reductase By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG3321.
HOGENOMiHOG000046292.
KOiK12441.
OMAiVAMFRPK.
OrthoDBiEOG6QP0WP.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXL9-1 [UniParc]FASTAAdd to Basket

« Hide

MMRTAFSRIS GMTAQQRTSL ADEFDRVSRI AVAEPVAVVG IGCRFPGDVD     50
GPESFWDFLV AGRNAISTVP ADRWDAEAFY HPDPLTPGRM TTKWGGFVPD 100
VAGFDAEFFG ITPREAAAMD PQQRMLLEVA WEALEHAGIP PDSLGGTRTA 150
VMMGVYFNEY QSMLAASPQN VDAYSGTGNA HSITVGRISY LLGLRGPAVA 200
VDTACSSSLV AVHLACQSLR LRETDLALAG GVSITLRPET QIAISAWGLL 250
SPQGRCAAFD AAADGFVRGE GAGVVVLKRL TDAVRDGDQV LAVVRGSAVN 300
QDGRSNGVTA PNTAAQCDVI ADALRSGDVA PDSVNYVEAH GTGTVLGDPI 350
EFEALAATYG HGGDACALGA VKTNIGHLEA AAGIAGFIKA TLAVQRATIP 400
PNLHFSQWNP AIDAASTRFF VPTQNSPWPT AEGPRRAAVS SFGLGGTNAH 450
VIIEQGSELA PVSEGGEDTG VSTLVVTGKT AQRMAATAQV LADWMEGPGA 500
EVAVADVAHT VNHHRARQAT FGTVVARDRA QAIAGLRALA AGQHAPGVVS 550
HQDGSPGPGT VFVYSGRGSQ WAGMGRQLLA DEPAFAAAVA ELEPVFVEQA 600
GFSLRDVIAT GKELVGIEQI QLGLIGMQLT LTELWRSYGV QPDLVIGHSM 650
GEVAAAVVAG ALTPAEGLRV TATRARLMAP LSGQGGMALL GLDAAATEAL 700
IADYPQVTVG IYNSPRQTVI AGPTEQIDEL IARVRAQNRF ASRVNIEVAP 750
HNPAMDALQP AMRSELADLT PRTPTIGIIS TTYADLHTQP IFDAEHWATN 800
MRNPVRFQQA IASAGSGADG AYHTFIEISA HPLLTQAIAD TLEDAHRPTK 850
SAAKYLSIGT LQRDADDTVT FRTNLYTADI AHPPHTCHPP EPHPTIPTTP 900
WQHTHHWIAT THPSTAAPED PGSNKVVVNG QSTSESRALE DWCHQLAWPI 950
RPAVSADPPS TAAWLVVADN ELCHELARAA DSRVDSLSPP ALAAGSDPAA 1000
LLDALRGVDN VLYAPPVPGE LLDIESAYQV FHATRRLAAA MVASSATAIS 1050
PPKLFIMTRN AQPISEGDRA NPGHAVLWGL GRSLALEHPE IWGGIIDLDD 1100
SMPAELAVRH VLTAAHGTDG EDQVVYRSGA RHVPRLQRRT LPGKPVTLNA 1150
DASQLVIGAT GNIGPHLIRQ LARMGAKTIV AMARKPGALD ELTQCLAATG 1200
TDLIAVAADA TDPAAMQTLF DRFGTELPPL EGIYLAAFAG RPALLSEMTD 1250
DDVTTMFRPK LDALALLHRL SLKSPVRHFV LFSSVSGLLG SRWLAHYTAT 1300
SAFLDSFAGA RRTMGLPATV VDWGLWKSLA DVQKDATQIS AESGLQPMAD 1350
EVAIGALPLV MNPDAAVATV VVAADWPLLA AAYRTRGALR IVDDLLPAPE 1400
DVGKGESEFR TSLRSCPAEK RRDMLFDHVG ALAATVMGMP PTEPLDPSAG 1450
FFQLGMDSLM SVTLQRALSE SLGEFLPASV VFDYPTVYSL TDYLATVLPE 1500
LLEIGATAVA TQQATDSYHE LTEAELLEQL SERLRGTQ 1538
Length:1,538
Mass (Da):162,485
Last modified:October 1, 2003 - v1
Checksum:iBD08742044AAFECC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44224.1.
RefSeqiNP_856602.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96644; CAD96644; Mb2957.
GeneIDi1092149.
KEGGimbo:Mb2957.
PATRICi18008226. VBIMycBov88188_3245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44224.1 .
RefSeqi NP_856602.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXL9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96644 ; CAD96644 ; Mb2957 .
GeneIDi 1092149.
KEGGi mbo:Mb2957.
PATRICi 18008226. VBIMycBov88188_3245.

Phylogenomic databases

eggNOGi COG3321.
HOGENOMi HOG000046292.
KOi K12441.
OMAi VAMFRPK.
OrthoDBi EOG6QP0WP.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
    Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
    J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPPSB_MYCBO
AccessioniPrimary (citable) accession number: Q7TXL9
Secondary accession number(s): X2BM25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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