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Q7TXL9

- PPSB_MYCBO

UniProt

Q7TXL9 - PPSB_MYCBO

Protein

Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB

Gene

ppsB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

    Cofactori

    Binds 1 phosphopantetheines covalently.By similarity
    NADP.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei205 – 2051For beta-ketoacyl synthase activityPROSITE-ProRule annotation
    Active sitei649 – 6491For malonyltransferase activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1153 – 119644NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
    2. oxidoreductase activity Source: UniProtKB-KW
    3. phosphopantetheine binding Source: InterPro

    GO - Biological processi

    1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
    2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
    3. phenolic phthiocerol biosynthetic process Source: UniProtKB
    4. phthiocerol biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsB
    Alternative name(s):
    Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
    Gene namesi
    Name:ppsB
    Ordered Locus Names:Mb2957
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. polyketide synthase complex Source: UniProtKB

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this genes abolish the production of phenolphthiocerol derivative (mycoside B) and phthiocerol dimycocerosates (DIM) on the cell envelope.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15381538Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsBPRO_0000406946Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1458 – 14581O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb2957.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TXL9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1409 – 149587Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 458423Beta-ketoacyl synthaseBy similarityAdd
    BLAST
    Regioni553 – 882330AcyltransferaseBy similarityAdd
    BLAST
    Regioni1153 – 1328176Beta-ketoacyl reductaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG3321.
    HOGENOMiHOG000046292.
    KOiK12441.
    OMAiVAMFRPK.
    OrthoDBiEOG6QP0WP.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view]
    SMARTiSM00827. PKS_AT. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 1 hit.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TXL9-1 [UniParc]FASTAAdd to Basket

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    MMRTAFSRIS GMTAQQRTSL ADEFDRVSRI AVAEPVAVVG IGCRFPGDVD     50
    GPESFWDFLV AGRNAISTVP ADRWDAEAFY HPDPLTPGRM TTKWGGFVPD 100
    VAGFDAEFFG ITPREAAAMD PQQRMLLEVA WEALEHAGIP PDSLGGTRTA 150
    VMMGVYFNEY QSMLAASPQN VDAYSGTGNA HSITVGRISY LLGLRGPAVA 200
    VDTACSSSLV AVHLACQSLR LRETDLALAG GVSITLRPET QIAISAWGLL 250
    SPQGRCAAFD AAADGFVRGE GAGVVVLKRL TDAVRDGDQV LAVVRGSAVN 300
    QDGRSNGVTA PNTAAQCDVI ADALRSGDVA PDSVNYVEAH GTGTVLGDPI 350
    EFEALAATYG HGGDACALGA VKTNIGHLEA AAGIAGFIKA TLAVQRATIP 400
    PNLHFSQWNP AIDAASTRFF VPTQNSPWPT AEGPRRAAVS SFGLGGTNAH 450
    VIIEQGSELA PVSEGGEDTG VSTLVVTGKT AQRMAATAQV LADWMEGPGA 500
    EVAVADVAHT VNHHRARQAT FGTVVARDRA QAIAGLRALA AGQHAPGVVS 550
    HQDGSPGPGT VFVYSGRGSQ WAGMGRQLLA DEPAFAAAVA ELEPVFVEQA 600
    GFSLRDVIAT GKELVGIEQI QLGLIGMQLT LTELWRSYGV QPDLVIGHSM 650
    GEVAAAVVAG ALTPAEGLRV TATRARLMAP LSGQGGMALL GLDAAATEAL 700
    IADYPQVTVG IYNSPRQTVI AGPTEQIDEL IARVRAQNRF ASRVNIEVAP 750
    HNPAMDALQP AMRSELADLT PRTPTIGIIS TTYADLHTQP IFDAEHWATN 800
    MRNPVRFQQA IASAGSGADG AYHTFIEISA HPLLTQAIAD TLEDAHRPTK 850
    SAAKYLSIGT LQRDADDTVT FRTNLYTADI AHPPHTCHPP EPHPTIPTTP 900
    WQHTHHWIAT THPSTAAPED PGSNKVVVNG QSTSESRALE DWCHQLAWPI 950
    RPAVSADPPS TAAWLVVADN ELCHELARAA DSRVDSLSPP ALAAGSDPAA 1000
    LLDALRGVDN VLYAPPVPGE LLDIESAYQV FHATRRLAAA MVASSATAIS 1050
    PPKLFIMTRN AQPISEGDRA NPGHAVLWGL GRSLALEHPE IWGGIIDLDD 1100
    SMPAELAVRH VLTAAHGTDG EDQVVYRSGA RHVPRLQRRT LPGKPVTLNA 1150
    DASQLVIGAT GNIGPHLIRQ LARMGAKTIV AMARKPGALD ELTQCLAATG 1200
    TDLIAVAADA TDPAAMQTLF DRFGTELPPL EGIYLAAFAG RPALLSEMTD 1250
    DDVTTMFRPK LDALALLHRL SLKSPVRHFV LFSSVSGLLG SRWLAHYTAT 1300
    SAFLDSFAGA RRTMGLPATV VDWGLWKSLA DVQKDATQIS AESGLQPMAD 1350
    EVAIGALPLV MNPDAAVATV VVAADWPLLA AAYRTRGALR IVDDLLPAPE 1400
    DVGKGESEFR TSLRSCPAEK RRDMLFDHVG ALAATVMGMP PTEPLDPSAG 1450
    FFQLGMDSLM SVTLQRALSE SLGEFLPASV VFDYPTVYSL TDYLATVLPE 1500
    LLEIGATAVA TQQATDSYHE LTEAELLEQL SERLRGTQ 1538
    Length:1,538
    Mass (Da):162,485
    Last modified:October 1, 2003 - v1
    Checksum:iBD08742044AAFECC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44224.1.
    RefSeqiNP_856602.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD96644; CAD96644; Mb2957.
    GeneIDi1092149.
    KEGGimbo:Mb2957.
    PATRICi18008226. VBIMycBov88188_3245.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44224.1 .
    RefSeqi NP_856602.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali Q7TXL9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb2957.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD96644 ; CAD96644 ; Mb2957 .
    GeneIDi 1092149.
    KEGGi mbo:Mb2957.
    PATRICi 18008226. VBIMycBov88188_3245.

    Phylogenomic databases

    eggNOGi COG3321.
    HOGENOMi HOG000046292.
    KOi K12441.
    OMAi VAMFRPK.
    OrthoDBi EOG6QP0WP.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00827. PKS_AT. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.
    2. "Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria."
      Azad A.K., Sirakova T.D., Fernandes N.D., Kolattukudy P.E.
      J. Biol. Chem. 272:16741-16745(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiPPSB_MYCBO
    AccessioniPrimary (citable) accession number: Q7TXL9
    Secondary accession number(s): X2BM25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3