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Q7TXL6

- PPSE_MYCBO

UniProt

Q7TXL6 - PPSE_MYCBO

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Protein

Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE

Gene

ppsE

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

Cofactori

Binds 1 phosphopantetheines covalently.By similarity
NADP.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei184 – 1841For beta-ketoacyl synthase activityPROSITE-ProRule annotation
Active sitei641 – 6411For malonyltransferase activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1286 – 133146NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. lipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsE
Ordered Locus Names:Mb2960
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the biosynthesis of phthiocerol/phenolphthiocerol dimycocerosate (DIM) and decreased the efficiency with which bacteria infected macrophages derived from monocytes (MDMs).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14881488Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsEPRO_0000406951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei965 – 9651O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2960.

Structurei

3D structure databases

ProteinModelPortaliQ7TXL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini935 – 100167Acyl carrierPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 389384Beta-ketoacyl synthaseBy similarityAdd
BLAST
Regioni551 – 868318AcyltransferaseBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1020.
HOGENOMiHOG000046292.
KOiK12444.
OMAiFDECATA.
OrthoDBiEOG6QP0WP.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00668. Condensation. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 3 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXL6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIPENAIAV VGMAGRFPGA KDVSAFWSNL RRGKESIVTL SEQELRDAGV
60 70 80 90 100
SDKTLADPAY VRRAPLLDGI DEFDAGFFGF PPLAAQVLDP QHRLFLQCAW
110 120 130 140 150
HALEDAGADP ARFDGSIGVY GTSSPSGYLL HNLLSHRDPN AVLAEGLNFD
160 170 180 190 200
QFSLFLQNDK DFLATRISHA FNLRGPSIAV QTACSSSLVA VHLACLSLLS
210 220 230 240 250
GECDMALAGG SSLCIPHRVG YFTSPGSMVS AVGHCRPFDV RADGTVFGSG
260 270 280 290 300
VGLVVLKPLA AAIDAGDRIH AVIRGSAINN DGSAKMGYAA PNPAAQADVI
310 320 330 340 350
AEAHAVSGID SSTVSYVECH GTGTPLGDPI EIQGLRAAFE VSQTSRSAPC
360 370 380 390 400
VLGSVKSNIG HLEVAAGIAG LIKTILCLKN KALPATLHYT SPNPELRLDQ
410 420 430 440 450
SPFVVQSKYG PWECDGVRRA GVSSFGVGGT NAHVVLEEAP AEASEVSAHA
460 470 480 490 500
EPAGPQVILL SAQTAAALGE SRTALAAALE TQDGPRLSDV AYTLARRRKH
510 520 530 540 550
NVTMAAVVHD REHAATVLRA AEHDNVFVGE AAHDGEHGDR ADAAPTSDRV
560 570 580 590 600
VFLFPGQGAQ HVGMAKGLYD TEPVFAQHFD TCAAGFRDET GIDLHAEVFD
610 620 630 640 650
GTATDLERID RSQPALFTVE YALAKLVDTF GVRAGAYIGY STGEYIAATL
660 670 680 690 700
AGVFDLQTAI KTVSLRARLM HESPPGAMVA VALGPDDVTQ YLPPEVELSA
710 720 730 740 750
VNDPGNCVVA GPKDQIRALR QRLTEAGIPV RRVRATHAFH TSAMDPMLGQ
760 770 780 790 800
FQEFLSRQQL RPPRTPLLSN LTGSWMSDQQ VVDPASWTRQ ISSPIRFADE
810 820 830 840 850
LDVVLAAPSR ILVEVGPGGS LTGSAMRHPK WSTTHRTVRL MRHPLQDVDD
860 870 880 890 900
RDTFLRALGE LWSAGVEVDW TPRRPAVPHL VSLPGYPFAR QRHWVEPNHT
910 920 930 940 950
VWAQAPGANN GSPAGTADGS TAATVDAARN GESQTEVTLQ RIWSQCLGVS
960 970 980 990 1000
SVDRNANFFD LGGDSLMAIS IAMAAANEGL TITPQDLYEY PTLASLTAAV
1010 1020 1030 1040 1050
DASFASSGLA KPPEAQANPA VPPNVTYFLD RGLRDTGRCR VPLILRLDPK
1060 1070 1080 1090 1100
IGLPDIRAVL TAVVNHHDAL RLHLVGNDGI WEQHIAAPAE FTGLSNRSVP
1110 1120 1130 1140 1150
DGVAAGSPEE RAAVLGILAE LLEDQTDPNA PLAAVHIAAA HGGPHYLCLA
1160 1170 1180 1190 1200
IHAMVTDDSS RQILATDIVT AFGQRLAGEE ITLEPVSTGW REWSLRCAAL
1210 1220 1230 1240 1250
ATHPAALDTR SYWIENSTKA TLWLADALPN AHTAHPPRAD ELTKLSSTLS
1260 1270 1280 1290 1300
VEQTSELDDG RRRFRRSIQT ILLAALGRTI AQTVGEGVVA VELEGEGRSV
1310 1320 1330 1340 1350
LRPDVDLRRT VGWFTTYYPV PLACATGLGA LAQLDAVHNT LKSVPHYGIG
1360 1370 1380 1390 1400
YGLLRYVYAP TGRVLGAQRT PDIHFRYAGV IPELPSGDAP VQFDSDMTLP
1410 1420 1430 1440 1450
VREPIPGMGH AIELRVYRFG GSLHLDWWYD TRRIPAATAE ALERTFPLAL
1460 1470 1480
SALIQEAIAA EHTEHDDSEI VGEPEAGALV DLSSMDAG
Length:1,488
Mass (Da):158,746
Last modified:October 1, 2003 - v1
Checksum:i14EC40432DB7F502
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44227.1.
RefSeqiNP_856605.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96647; CAD96647; Mb2960.
GeneIDi1092146.
KEGGimbo:Mb2960.
PATRICi18008232. VBIMycBov88188_3248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44227.1 .
RefSeqi NP_856605.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXL6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96647 ; CAD96647 ; Mb2960 .
GeneIDi 1092146.
KEGGi mbo:Mb2960.
PATRICi 18008232. VBIMycBov88188_3248.

Phylogenomic databases

eggNOGi COG1020.
HOGENOMi HOG000046292.
KOi K12444.
OMAi FDECATA.
OrthoDBi EOG6QP0WP.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00668. Condensation. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 3 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids."
    Astarie-Dequeker C., Le Guyader L., Malaga W., Seaphanh F.K., Chalut C., Lopez A., Guilhot C.
    PLoS Pathog. 5:E1000289-E1000289(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: BCG / Pasteur.

Entry informationi

Entry nameiPPSE_MYCBO
AccessioniPrimary (citable) accession number: Q7TXL6
Secondary accession number(s): X2BLX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: October 1, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3