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Q7TXL6

- PPSE_MYCBO

UniProt

Q7TXL6 - PPSE_MYCBO

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Protein
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE
Gene
ppsE, Mb2960
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids or p-hydroxyphenylalkanoic acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol and phenolphthiocerol derivatives, respectively.1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Cofactori

Binds 1 phosphopantetheines covalently By similarity.
NADP By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei184 – 1841For beta-ketoacyl synthase activity By similarity
Active sitei641 – 6411For malonyltransferase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1286 – 133146NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. lipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsE
Ordered Locus Names:Mb2960
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes abolish the biosynthesis of phthiocerol/phenolphthiocerol dimycocerosate (DIM) and decreased the efficiency with which bacteria infected macrophages derived from monocytes (MDMs).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14881488Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsE
PRO_0000406951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei965 – 9651O-(pantetheine 4'-phosphoryl)serine By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2960.

Structurei

3D structure databases

ProteinModelPortaliQ7TXL6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini935 – 100167Acyl carrier
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 389384Beta-ketoacyl synthase By similarity
Add
BLAST
Regioni551 – 868318Acyltransferase By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1020.
HOGENOMiHOG000046292.
KOiK12444.
OMAiFDECATA.
OrthoDBiEOG6QP0WP.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF00668. Condensation. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 3 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TXL6-1 [UniParc]FASTAAdd to Basket

« Hide

MSIPENAIAV VGMAGRFPGA KDVSAFWSNL RRGKESIVTL SEQELRDAGV     50
SDKTLADPAY VRRAPLLDGI DEFDAGFFGF PPLAAQVLDP QHRLFLQCAW 100
HALEDAGADP ARFDGSIGVY GTSSPSGYLL HNLLSHRDPN AVLAEGLNFD 150
QFSLFLQNDK DFLATRISHA FNLRGPSIAV QTACSSSLVA VHLACLSLLS 200
GECDMALAGG SSLCIPHRVG YFTSPGSMVS AVGHCRPFDV RADGTVFGSG 250
VGLVVLKPLA AAIDAGDRIH AVIRGSAINN DGSAKMGYAA PNPAAQADVI 300
AEAHAVSGID SSTVSYVECH GTGTPLGDPI EIQGLRAAFE VSQTSRSAPC 350
VLGSVKSNIG HLEVAAGIAG LIKTILCLKN KALPATLHYT SPNPELRLDQ 400
SPFVVQSKYG PWECDGVRRA GVSSFGVGGT NAHVVLEEAP AEASEVSAHA 450
EPAGPQVILL SAQTAAALGE SRTALAAALE TQDGPRLSDV AYTLARRRKH 500
NVTMAAVVHD REHAATVLRA AEHDNVFVGE AAHDGEHGDR ADAAPTSDRV 550
VFLFPGQGAQ HVGMAKGLYD TEPVFAQHFD TCAAGFRDET GIDLHAEVFD 600
GTATDLERID RSQPALFTVE YALAKLVDTF GVRAGAYIGY STGEYIAATL 650
AGVFDLQTAI KTVSLRARLM HESPPGAMVA VALGPDDVTQ YLPPEVELSA 700
VNDPGNCVVA GPKDQIRALR QRLTEAGIPV RRVRATHAFH TSAMDPMLGQ 750
FQEFLSRQQL RPPRTPLLSN LTGSWMSDQQ VVDPASWTRQ ISSPIRFADE 800
LDVVLAAPSR ILVEVGPGGS LTGSAMRHPK WSTTHRTVRL MRHPLQDVDD 850
RDTFLRALGE LWSAGVEVDW TPRRPAVPHL VSLPGYPFAR QRHWVEPNHT 900
VWAQAPGANN GSPAGTADGS TAATVDAARN GESQTEVTLQ RIWSQCLGVS 950
SVDRNANFFD LGGDSLMAIS IAMAAANEGL TITPQDLYEY PTLASLTAAV 1000
DASFASSGLA KPPEAQANPA VPPNVTYFLD RGLRDTGRCR VPLILRLDPK 1050
IGLPDIRAVL TAVVNHHDAL RLHLVGNDGI WEQHIAAPAE FTGLSNRSVP 1100
DGVAAGSPEE RAAVLGILAE LLEDQTDPNA PLAAVHIAAA HGGPHYLCLA 1150
IHAMVTDDSS RQILATDIVT AFGQRLAGEE ITLEPVSTGW REWSLRCAAL 1200
ATHPAALDTR SYWIENSTKA TLWLADALPN AHTAHPPRAD ELTKLSSTLS 1250
VEQTSELDDG RRRFRRSIQT ILLAALGRTI AQTVGEGVVA VELEGEGRSV 1300
LRPDVDLRRT VGWFTTYYPV PLACATGLGA LAQLDAVHNT LKSVPHYGIG 1350
YGLLRYVYAP TGRVLGAQRT PDIHFRYAGV IPELPSGDAP VQFDSDMTLP 1400
VREPIPGMGH AIELRVYRFG GSLHLDWWYD TRRIPAATAE ALERTFPLAL 1450
SALIQEAIAA EHTEHDDSEI VGEPEAGALV DLSSMDAG 1488
Length:1,488
Mass (Da):158,746
Last modified:October 1, 2003 - v1
Checksum:i14EC40432DB7F502
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44227.1.
RefSeqiNP_856605.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96647; CAD96647; Mb2960.
GeneIDi1092146.
KEGGimbo:Mb2960.
PATRICi18008232. VBIMycBov88188_3248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44227.1 .
RefSeqi NP_856605.1. NC_002945.3.

3D structure databases

ProteinModelPortali Q7TXL6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 233413.Mb2960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96647 ; CAD96647 ; Mb2960 .
GeneIDi 1092146.
KEGGi mbo:Mb2960.
PATRICi 18008232. VBIMycBov88188_3248.

Phylogenomic databases

eggNOGi COG1020.
HOGENOMi HOG000046292.
KOi K12444.
OMAi FDECATA.
OrthoDBi EOG6QP0WP.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR001242. Condensatn.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF00668. Condensation. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 3 hits.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids."
    Astarie-Dequeker C., Le Guyader L., Malaga W., Seaphanh F.K., Chalut C., Lopez A., Guilhot C.
    PLoS Pathog. 5:E1000289-E1000289(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE PHTHIOCEROL AND PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: BCG / Pasteur.

Entry informationi

Entry nameiPPSE_MYCBO
AccessioniPrimary (citable) accession number: Q7TXL6
Secondary accession number(s): X2BLX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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