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Q7TXK8

- MSL7_MYCBO

UniProt

Q7TXK8 - MSL7_MYCBO

Protein

Phenolphthiocerol synthesis polyketide synthase type I Pks15/1

Gene

pks15/1

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the elongation by iterative transfer of p-hydroxybenzoyl group from FadD22 (pHBA-S-FAdD22) to form p-hydroxyphenylalkanoate (pHPA) intermediates during phenolphthiocerol (PPOL) biosynthesis. PPOL is an important intermediate in the biosynthesis of phenolic glycolipid (mycosid B).1 Publication

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

    Cofactori

    Binds 1 phosphopantetheine covalently.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei216 – 2161For beta-ketoacyl synthase activityPROSITE-ProRule annotation
    Active sitei670 – 6701For acyltransferase activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1536 – 155318NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1725 – 174016NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
    2. cofactor binding Source: InterPro
    3. oxidoreductase activity Source: InterPro
    4. phosphopantetheine binding Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
    2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
    3. lipid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17228.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenolphthiocerol synthesis polyketide synthase type I Pks15/1
    Alternative name(s):
    Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
    Gene namesi
    Name:pks15/1
    Synonyms:msl7, pks1
    Ordered Locus Names:Mb2971c
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. polyketide synthase complex Source: UniProtKB

    Pathology & Biotechi

    Disruption phenotypei

    Disruption of pks15/1 abolishes the production of phenolphthiocerol.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21122112Phenolphthiocerol synthesis polyketide synthase type I Pks15/1PRO_0000406362Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2045 – 20451O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb2971c.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TXK8.
    SMRiQ7TXK8. Positions 18-921.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2012 – 208271Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni42 – 471430Beta-ketoacyl synthaseBy similarityAdd
    BLAST
    Regioni579 – 893315AcyltransferaseBy similarityAdd
    BLAST
    Regioni941 – 1101161DehydrataseBy similarityAdd
    BLAST
    Regioni1406 – 1711306EnoylreductaseBy similarityAdd
    BLAST
    Regioni1724 – 1905182Beta-ketoacyl reductaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the beta-ketoacyl-ACP synthases family.Curated
    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3321.
    HOGENOMiHOG000046292.
    KOiK12430.
    OMAiHAHAVES.
    OrthoDBiEOG6QP0WP.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 2 hits.
    3.90.180.10. 1 hit.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR020807. PKS_dehydratase.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view]
    SMARTiSM00827. PKS_AT. 1 hit.
    SM00826. PKS_DH. 1 hit.
    SM00829. PKS_ER. 1 hit.
    SM00822. PKS_KR. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 1 hit.
    [Graphical view]
    SUPFAMiSSF101173. SSF101173. 1 hit.
    SSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 1 hit.
    SSF55048. SSF55048. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TXK8-1 [UniParc]FASTAAdd to Basket

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    MIEEQRTMSV EGADQQSEKL FHYLKKVAVE LDETRARLRE YEQRATEPVA     50
    VVGIGCRFPG GVDGPDGLWD VVSAGRDVVS EFPTDRGWDV EGLYDPDPDA 100
    EGKTYTRWGA FLDDATGFDA GFFGIAPSEV LAMDPQQRLM LEVSWEALEH 150
    AGIDPLSLRG SATGVYTGIF AASYGNRDTG GLQGYGLTGT SISVASGRVS 200
    YVLGLQGPAV SVDTACSSSL VAIHWAMSSL RSGECDLALA GGVTVMGLPS 250
    IFVGFSRQRG LAADGRCKAF AAAADGTGWG EGAGVVVLER LSDARRLGHS 300
    VLAVVRGSAV NQDGASNGLT APNGLAQQRV IQAALANAGL SAADVDVVEA 350
    HGTATTLGDP IEAQALLSTY GQGRPAEQPL WVGSIKSNMG HTQAAAGVAG 400
    VIKMVQAMRH GVMPATLHVD EPSPRVDWTS GAVSVLTEAR EWSVDGRPRR 450
    AAVSSFGISG TNAHLILEEA PVPAPAEAPV EASESTGGPR PSMVPWVISA 500
    RSAEALTAQA GRLMAHVQAN PGLDPIDVGC SLASRSVFEH RAVVVGASRE 550
    QLIAGLAGLA AGEPGAGVAV GQPGSVGKTV VVFPGQGAQR IGMGRELYGE 600
    LPVFAQAFDA VADELDRHLR LPLRDVIWGA DADLLDSTEF AQPALFAVEV 650
    ASFAVLRDWG VLPDFVMGHS VGELAAAHAA GVLTLADAAM LVVARGRLMQ 700
    ALPAGGAMVA VAASEDEVEP LLGEGVGIAA INAPESVVIS GAQAAANAIA 750
    DRFAAQGRRV HQLAVSHAFH SPLMEPMLEE FARVAARVQA REPQLGLVSN 800
    VTGELAGPDF GSAQYWVDHV RRPVRFADSA RHLQTLGATH FIEAGPGSGL 850
    TGSIEQSLAP AEAMVVSMLG KDRPELASAL GAAGQVFTTG VPVQWSAVFA 900
    GSGGRRVQLP TYAFQRRRFW ETPGADGPAD AAGLGLGATE HALLGAVVER 950
    PDSDEVVLTG RLSLADQPWL ADHVVNGVVL FPGAGFVELV IRAGDEVGCA 1000
    LIEELVLAAP LVMHPGVGVQ VQVVVGAADE SGHRAVSVYS RGDQSQGWLL 1050
    NAEGMLGVAA AETPMDLSVW PPEGAESVDI SDGYAQLAER GYAYGPAFQG 1100
    LVAIWRRGSE LFAEVVAPGE AGVAVDRMGM HPAVLDAVLH ALGLAVEKTQ 1150
    ASTETRLPFC WRGVSLHAGG AGRVRARFAS AGADAISVDV CDATGLPVLT 1200
    VRSLVTRPIT AEQLRAAVTA AGGASDQGPL EVVWSPISVV SGGANGSAPP 1250
    APVSWADFCA GSDGDASVVV WELESAGGQA SSVVGSVYAA THTALEVLQS 1300
    WLGADRAATL VVLTHGGVGL AGEDISDLAA AAVWGMARSA QAENPGRIVL 1350
    IDTDAAVDAS VLAGVGEPQL LVRGGTVHAP RLSPAPALLA LPAAESAWRL 1400
    AAGGGGTLED LVIQPCPEVQ APLQAGQVRV AVAAVGVNFR DVVAALGMYP 1450
    GQAPPLGAEG AGVVLETGPE VTDLAVGDAV MGFLGGAGPL AVVDQQLVTR 1500
    VPQGWSFAQA AAVPVVFLTA WYGLADLAEI KAGESVLIHA GTGGVGMAAV 1550
    QLARQWGVEV FVTASRGKWD TLRAMGFDDD HIGDSRTCEF EEKFLAVTEG 1600
    RGVDVVLDSL AGEFVDASLR LLVRGGRFLE MGKTDIRDAQ EIAANYPGVQ 1650
    YRAFDLSEAG PARMQEMLAE VRELFDTREL HRLPVTTWDV RCAPAAFRFM 1700
    SQARHIGKVV LTMPSALADR LADGTVVITG ATGAVGGVLA RHLVGAYGVR 1750
    HLVLASRRGD RAEGAAELAA DLTEAGAKGQ VVACDVADRA AVAGLFAQLS 1800
    REYPPVRGVI HAAGVLDDAV ITSLTPDRID TVLRAKVDAA WNLHQATSDL 1850
    DLSMFVLCSS IAATVGSPGQ GNYSAANAFL DGLAAHRQAA GLAGISLAWG 1900
    LWEQPGGMTA HLSSRDLARM SRSGLAPMSP AEAVELFDAA LAIDHPLAVA 1950
    TLLDRAALDA RAQAGALPAL FSGLARRPRR RQIDDTGDAT SSKSALAQRL 2000
    HGLAADEQLE LLVGLVCLQA AAVLGRPSAE DVDPDTEFGD LGFDSLTAVE 2050
    LRNRLKTATG LTLPPTVIFD HPTPTAVAEY VAQQMSGSRP TESGDPTSQV 2100
    VEPAAAEVSV HA 2112
    Length:2,112
    Mass (Da):218,304
    Last modified:October 1, 2003 - v1
    Checksum:iDCB5FA31A58A2785
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44238.1.
    RefSeqiNP_856616.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD96658; CAD96658; Mb2971c.
    GeneIDi1092134.
    KEGGimbo:Mb2971c.
    PATRICi18008262. VBIMycBov88188_3263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44238.1 .
    RefSeqi NP_856616.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali Q7TXK8.
    SMRi Q7TXK8. Positions 18-921.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb2971c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD96658 ; CAD96658 ; Mb2971c .
    GeneIDi 1092134.
    KEGGi mbo:Mb2971c.
    PATRICi 18008262. VBIMycBov88188_3263.

    Phylogenomic databases

    eggNOGi COG3321.
    HOGENOMi HOG000046292.
    KOi K12430.
    OMAi HAHAVES.
    OrthoDBi EOG6QP0WP.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci MetaCyc:MONOMER-17228.

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 2 hits.
    3.40.50.720. 2 hits.
    3.90.180.10. 1 hit.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR020807. PKS_dehydratase.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00827. PKS_AT. 1 hit.
    SM00826. PKS_DH. 1 hit.
    SM00829. PKS_ER. 1 hit.
    SM00822. PKS_KR. 1 hit.
    SM00825. PKS_KS. 1 hit.
    SM00823. PKS_PP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101173. SSF101173. 1 hit.
    SSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 1 hit.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 1 hit.
    SSF55048. SSF55048. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.
    2. "Role of the pks15/1 gene in the biosynthesis of phenolglycolipids in the Mycobacterium tuberculosis complex. Evidence that all strains synthesize glycosylated p-hydroxybenzoic methyl esters and that strains devoid of phenolglycolipids harbor a frameshift mutation in the pks15/1 gene."
      Constant P., Perez E., Malaga W., Laneelle M.A., Saurel O., Daffe M., Guilhot C.
      J. Biol. Chem. 277:38148-38158(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHENOLPHTHIOCEROL BIOSYNTHESIS, DISRUPTION PHENOTYPE.
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiMSL7_MYCBO
    AccessioniPrimary (citable) accession number: Q7TXK8
    Secondary accession number(s): X2BMA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3