Q7TXA9 (PKNK_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknK
EC=2.7.11.1

Alternative name(s):
Protein kinase K

Gene names

Name:	pknK
Ordered Locus Names:	Mb3107c

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	1110 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1110	1110	Serine/threonine-protein kinase PknK		PRO_0000171223
Regions
	Domain	26 – 283	258	Protein kinase
	Nucleotide binding	32 – 40	9	ATP By similarity
Sites
	Active site	148	1	Proton acceptor By similarity
	Metal binding	154	1	Magnesium By similarity
	Metal binding	167	1	Magnesium By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TXA9 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 3C2AD79972BCCDA0	FASTA	1,110	119,446
	10 20 30 40 50 60 MTDVDPHATR RDLVPNIPAE LLEAGFDNVE EIGRGGFGVV YRCVQPSLDR AVAVKVLSTD 70 80 90 100 110 120 LDRDNLERFL REQRAMGRLS GHPHIVTVLQ VGVLAGGRPF IVMPYHAKNS LETLIRRHGP 130 140 150 160 170 180 LDWRETLSIG VKLAGALEAA HRVGTLHRDV KPGNILLTDY GEPQLTDFGI ARIAGGFETA 190 200 210 220 230 240 TGVIAGSPAF TAPEVLEGAS PTPASDVYSL GATLFCVLTG HAAYERRSGE RVIAQFLRIT 250 260 270 280 290 300 SQPIPDLRKQ GLPADVAAAI ERAMARHPAD RPATAADVGE ELRDVQRRNG VSVDEMPLPV 310 320 330 340 350 360 ELGVERRRSP EAHAAHRHTG GGTPTVPTPP TPATKYRPSV PTGSLVTRSR LTDILRAGGR 370 380 390 400 410 420 RRLILIHAPS GFGKSTLAAQ WREELSRDGA AVAWLTIDND DNNEVWFLSH LLESIRRVRP 430 440 450 460 470 480 TLAESLGHVL EEHGDDAGRY VLTSLIDEIH ENDDRIAVVI DDWHRVSDSR TQAALGFLLD 490 500 510 520 530 540 NGCHHLQLIV TSWSRAGLPV GRLRIGDELA EIDSAALRFD TDEAAALLND AGGLRLPRAD 550 560 570 580 590 600 VQALTTSTDG WAAALRLAAL SLRGGGDATQ LLRGLSGASD VIHEFLSENV LDTLEPELRE 610 620 630 640 650 660 FLLVASVTER TCGGLASALA GITNGRAMLE EAEHRGLFLQ RTEDDPNWFR FHQMFADFLH 670 680 690 700 710 720 RRLERGGSHR VAELHRRASA WFAENGYLHE AVDHALAAGD PARAVDLVEQ DETNLPEQSK 730 740 750 760 770 780 MTTLLAIVQK LPTSMVVSRA RLQLAIAWAN ILLQRPAPAT GALNRFETAL GRAELPEATQ 790 800 810 820 830 840 ADLRAEADVL RAVAEVFADR VERVDDLLAE AMSRPDTLPP RVPGTAGNTA ALAAICRFEF 850 860 870 880 890 900 AEVYPLLDWA APYQEMMGPF GTVYAQCLRG MAARNRLDIV AALQNFRTAF EVGTAVGAHS 910 920 930 940 950 960 HAARLAGSLL AELLYETGDL AGAGRLMDES YLLGSEGGAV DYLAARYVIG ARVKAAQGDH 970 980 990 1000 1010 1020 EGAADRLSTG GDTAVQLGLP RLAARINNER IRLGIALPAA VAADLLAPRT IPRDNGIATM 1030 1040 1050 1060 1070 1080 TAELDEDSAV RLLSAGDSAD RDQACQRAGA LAAAIDGTRR PLAALQAQIL HIETLAATGR 1090 1100 1110 ESDARNELAP VATKCAELGL SRLLVDAGLA

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248344 Genomic DNA. Translation: CAD96794.1.
RefSeq	NP_856752.1. NC_002945.3.
3D structure databases
ProteinModelPortal	Q7TXA9.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb3107c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD96794; CAD96794; Mb3107c.
GeneID	1093286.
KEGG	mbo:Mb3107c.
PATRIC	18008570. VBIMycBov88188_3414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000074403.
KO	K13419.
OMA	RPYIVMQ.
ProtClustDB	CLSK792267.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR016236. Ser/Thr_kinase_PknK_prd. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000574. Ser/Thr_PK_PknK_prd. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNK_MYCBO

Accession

Primary (citable) accession number: Q7TXA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program