Q7TWL9 (DNAE2_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Mb3405c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.
Sequence caution	The sequence CAD95567.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1091	1091	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103383

Sequences

	Sequence		Length	Mass (Da)
	Q7TWL9 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: F4973A856D56711D	FASTA	1,091	117,784
	10 20 30 40 50 60 MGWSNGPPSW AEMERVLNGK PRHAGVPAFD ADGDVPRSRK RGAYQPPGRE RVGSSVAYAE 70 80 90 100 110 120 LHAHSAYSFL DGASTPEELV EEAARLGLCA LALTDHDGLY GAVRFAEAAA ELDVRTVFGA 130 140 150 160 170 180 ELSLGATART ERPDPPGPHL LVLARGPEGY RRLSRQLAAA HLAGGEKGKP RYDFDALTEA 190 200 210 220 230 240 AGGHWHILTG CRKGHVRQAL SQGGPAAAQR ALADLVDRFT PSRVSIELTH HGHPLDDERN 250 260 270 280 290 300 AALAGLAPRF GVGIVATTGA HFADPSRGRL AMAMAAIRAR RSLDSAAGWL APLGGAHLRS 310 320 330 340 350 360 GEEMARLFAW CPEAVTAAAE LGERCAFGLQ LIAPRLPPFD VPDGHTEDSW LRSLVMAGAR 370 380 390 400 410 420 ERYGPPKSAP RAYSQIEHEL KVIAQLRFPG YFLVVHDITR FCRDNDILCQ GRGSAANSAV 430 440 450 460 470 480 CYALGVTAVD PVANELLFER FLSPARDGPP DIDIDIESDQ REKVIQYVYH KYGRDYAAQV 490 500 510 520 530 540 ANVITYRGRS AVRDMARALG FSPGQQDAWS KQVSHWTGQA DDVDGIPEQV IDLATQIRNL 550 560 570 580 590 600 PRHLGIHSGG MVICDRPIAD VCPVEWARMA NRSVLQWDKD DCAAIGLVKF DLLGLGMLSA 610 620 630 640 650 660 LHYAKDLVAE HKGIEVDLAR LDLSEPAVYE MLARADSVGV FQVESRAQMA TLPRLKPRVF 670 680 690 700 710 720 YDLVVEVALI RPGPIQGGSV HPYIRRRNGV DPVIYEHPSM APALRKTLGV PLFQEQLMQL 730 740 750 760 770 780 AVDCAGFSAA EADQLRRAMG SKRSTERMRR LRGRFYDGMR ALHGAPDEVI DRIYEKLEAF 790 800 810 820 830 840 ANFGFPESHA LSFASLVFYS AWFKLHHPAA FCAALLRAQP MGFYSPQSLV ADARRHGVAV 850 860 870 880 890 900 HGPCVNASLA HATCENAGTE VRLGLGAVRY LGAELAEKLV AERTANGPFT SLPDLTSRVQ 910 920 930 940 950 960 LSVPQVEALA TAGALGCFGM SRREALWAAG AAATGRPDRL PGVGSSSHIP ALPGMSELEL 970 980 990 1000 1010 1020 AAADVWATGV SPDSYPTQFL RADLDAMGVL PAERLGSVSD GDRVLIAGAV THRQRPATAQ 1030 1040 1050 1060 1070 1080 GVTFINLEDE TGMVNVLCTP GVWARHRKLA HTAPALLIRG QVQNASGAIT VVAERMGRLT 1090 LAVGARSRDF R

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248345 Genomic DNA. Translation: CAD95567.1. Different initiation.
RefSeq	NP_857046.2. NC_002945.3.
3D structure databases
ProteinModelPortal	Q7TWL9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000016192; EBMYCP00000016027; EBMYCG00000016187.
GeneID	1090941.
GenomeReviews	Gene locus Mb3405c in contig BX248333_GR.
KEGG	mbo:Mb3405c.
PATRIC	18009224. VBIMycBov88188_3740.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016568.
HOGENOM	HBG734490.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	MBOV233413:MB3405C-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_MYCBO

Accession

Primary (citable) accession number: Q7TWL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program