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Protein

Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligase

Gene

fadD19

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the activation of long-chain fatty acids as acyl-coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. Also involved in the degradation of cholesterol via the degradation of the side chains of C-24 branched-chain sterols. Catalyzes the ATP-dependent CoA thioesterification of the sterol 3-oxocholest-4-en-26-oate to yield 3-oxocholest-4-en-26-oyl-CoA.By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.By similarity
ATP + 3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei415 – 4151ATPBy similarity
Binding sitei430 – 4301ATPBy similarity
Binding sitei521 – 5211ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 1829ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-UniPathway
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cholesterol metabolism, Fatty acid metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00094.
UPA00296.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligaseBy similarity (EC:6.2.1.3By similarity, EC:6.2.1.42By similarity)
Short name:
FACLBy similarity
Alternative name(s):
Acyl-CoA synthetaseBy similarity
Steroid-CoA ligaseBy similarity
Steroid-coenzyme A ligaseBy similarity
Gene namesi
Name:fadD19By similarity
Ordered Locus Names:Mb3544c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligasePRO_0000406791Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb3544c.

Structurei

3D structure databases

ProteinModelPortaliQ7TWB7.
SMRiQ7TWB7. Positions 12-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000230015.
KOiK18688.
OMAiMIHGGSQ.
OrthoDBiEOG6MSRZN.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TWB7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVALNIADL AEHAIDAVPD RVAVICGDEQ LTYAQLEDKA NRLAHHLIDQ
60 70 80 90 100
GVQKDDKVGL YCRNRIEIVI AMLGIVKAGA ILVNVNFRYV EGELRYLFDN
110 120 130 140 150
SDMVALVHER RYADRVANVL PDTPHVRTIL VVEDGSDQDY RRYGGVEFYS
160 170 180 190 200
AIAAGSPERD FGERSADAIY LLYTGGTTGF PKGVMWRHED IYRVLFGGTD
210 220 230 240 250
FATGEFVKDE YDLAKAAAAN PPMIRYPIPP MIHGATQSAT WMALFSGQTT
260 270 280 290 300
VLAPEFNADE VWRTIHKHKV NLLFFTGDAM ARPLVDALVK GNDYDLSSLF
310 320 330 340 350
LLASTAALFS PSIKEKLLEL LPNRVITDSI GSSETGFGGT SVVAAGQAHG
360 370 380 390 400
GGPRVRIDHR TVVLDDDGNE VKPGSGMRGV IAKKGNIPVG YYKDEKKTAE
410 420 430 440 450
TFRTINGVRY AIPGDYAQVE EDGTVTMLGR GSVSINSGGE KVYPEEVEAA
460 470 480 490 500
LKGHPDVFDA LVVGVPDPRY GQQVAAVVQA RPGCRPSLAE LDSFVRSEIA
510 520 530 540
GYKVPRSLWF VDEVKRSPAG KPDYRWAKEQ TEARPADDVH AGHVTSGG
Length:548
Mass (Da):59,741
Last modified:October 1, 2003 - v1
Checksum:iE574B4BFA86F324E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44814.1.
RefSeqiNP_857183.1. NC_002945.3.

Genome annotation databases

GeneIDi1093161.
KEGGimbo:Mb3544c.
PATRICi18009510. VBIMycBov88188_3883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44814.1.
RefSeqiNP_857183.1. NC_002945.3.

3D structure databases

ProteinModelPortaliQ7TWB7.
SMRiQ7TWB7. Positions 12-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233413.Mb3544c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1093161.
KEGGimbo:Mb3544c.
PATRICi18009510. VBIMycBov88188_3883.

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000230015.
KOiK18688.
OMAiMIHGGSQ.
OrthoDBiEOG6MSRZN.

Enzyme and pathway databases

UniPathwayiUPA00094.
UPA00296.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiFAC19_MYCBO
AccessioniPrimary (citable) accession number: Q7TWB7
Secondary accession number(s): X2BNN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2003
Last modified: February 4, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.