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Q7TVB0 (GSHB_PROMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:Pro_0203
OrganismProchlorococcus marinus (strain SARG / CCMP1375 / SS120) [Reference proteome] [HAMAP]
Taxonomic identifier167539 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197473

Regions

Domain120 – 304185ATP-grasp
Nucleotide binding146 – 20257ATP By similarity

Sites

Metal binding2751Magnesium or manganese By similarity
Metal binding2771Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7TVB0 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 9AFFE358F9776F5D

FASTA30833,535
        10         20         30         40         50         60 
MKQLFILDPI ELINPAKDSS AALMQAAHRA SLDIWVCTPA DLQAKGAKAR VVAKTIVPEP 

        70         80         90        100        110        120 
WKSLGEAQNL SLNEFHCIWM RKDPPVDEAF LYATHLLEVA ERAGVLVLNK PASLRAWNEK 

       130        140        150        160        170        180 
LGALRFSKLM APTLVASRVN ELKEFAKEQG EVVLKPLGGK GGQGVIRVAD AALGLDALLE 

       190        200        210        220        230        240 
LVTSQEQMPV MMQKFLPKVI EGDKRILLVN GEPLGAINRL PKCGDFRSNL ALGGQAEATN 

       250        260        270        280        290        300 
LSKREKEICE ELGPSLKSEG LFFVGIDVIG GMLSEINVTS PTGVREVEKL MNIPLADQVI 


ERLLANFS 

« Hide

References

[1]"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome."
Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. expand/collapse author list , Wincker P., Wolf Y.I., Hess W.R.
Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017126 Genomic DNA. Translation: AAP99249.1.
RefSeqNP_874597.1. NC_005042.1.

3D structure databases

ProteinModelPortalQ7TVB0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167539.Pro0203.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP99249; AAP99249; Pro_0203.
GeneID1461582.
KEGGpma:Pro_0203.
PATRIC23026993. VBIProMar8617_0210.

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMAEISTKQG.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycPMAR167539:GJN2-206-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_PROMA
AccessionPrimary (citable) accession number: Q7TVB0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways