Q7TVB0 (GSHB_PROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	Pro_0203

Organism

Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)

Taxonomic identifier

167539 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutathione synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glutathione synthetase HAMAP MF_00162		PRO_0000197473
Regions
	Domain	120 – 304	185	ATP-grasp
	Nucleotide binding	146 – 202	57	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TVB0 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 9AFFE358F9776F5D	FASTA	308	33,535
	10 20 30 40 50 60 MKQLFILDPI ELINPAKDSS AALMQAAHRA SLDIWVCTPA DLQAKGAKAR VVAKTIVPEP 70 80 90 100 110 120 WKSLGEAQNL SLNEFHCIWM RKDPPVDEAF LYATHLLEVA ERAGVLVLNK PASLRAWNEK 130 140 150 160 170 180 LGALRFSKLM APTLVASRVN ELKEFAKEQG EVVLKPLGGK GGQGVIRVAD AALGLDALLE 190 200 210 220 230 240 LVTSQEQMPV MMQKFLPKVI EGDKRILLVN GEPLGAINRL PKCGDFRSNL ALGGQAEATN 250 260 270 280 290 300 LSKREKEICE ELGPSLKSEG LFFVGIDVIG GMLSEINVTS PTGVREVEKL MNIPLADQVI ERLLANFS

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017126 Genomic DNA. Translation: AAP99249.1.
RefSeq	NP_874597.1. NC_005042.1.
3D structure databases
ProteinModelPortal	Q7TVB0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1461582.
GenomeReviews	Gene locus Pro_0203 in contig AE017126_GR.
KEGG	pma:Pro0203.
PATRIC	23026993. VBIProMar8617_0210.
Phylogenomic databases
HOGENOM	HBG624526.
OMA	HLTEINV.
ProtClustDB	PRK05246.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_0203-MONOMER.
Family and domain databases
HAMAP	MF_00162. GSH-S. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01920.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. Glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_PROMA

Accession

Primary (citable) accession number: Q7TVB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program