Q7TUT7 (SYE_PROMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	PMT_1308

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119625
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TUT7 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 9891E085D9569A1D	FASTA	476	53,387
	10 20 30 40 50 60 MKVRVRLAPS PTGTLHIGTA RTAVFNWLFA RHQGGQFLLR IEDTDKERSK PEFTTNILEG 70 80 90 100 110 120 LKWLGLNWDE QPIIQSQHVD DHRAAIQKLL DRDLAYRCYA SETELEAMRE TQKAQGKAPR 130 140 150 160 170 180 YDNRHRDLNP EQEAAFQSEG RTAVVRFRID DDATISWKDL VRGPMHWKGS DLGGDMVISR 190 200 210 220 230 240 RAPAKEIGDP LYNLVVVVDD AAMSISHVIR GEDHIANTAK QLLIYEALGL PIPQFAHTPL 250 260 270 280 290 300 ILNSEGRKLS KRDGVTSISD FQAMGYTAEA MANYMSLLGW SVPEGTDERF TLQQAATVFS 310 320 330 340 350 360 FDRVNKAGAK FDWDKLNWLN SQVLHDLPKD QLLHELKPLW SKAGWALPEE NWCLDLAELL 370 380 390 400 410 420 GPSLTLLKDG VDQARPFFEE PTLQADGLEQ LAVDGAKAGL SNLLDQLDRT SWDGFDVKQA 430 440 450 460 470 QQLLTNAAQA ANVKKGVIMK SLRAALLGRL QGPDLITTWG LLARIGQDLN RLRRCL

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9313.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548175 Genomic DNA. Translation: CAE21483.1.
RefSeq	NP_895136.1. NC_005071.1.
3D structure databases
ProteinModelPortal	Q7TUT7.
SMR	Q7TUT7. Positions 3-476.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TUT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1729270.
GenomeReviews	Gene locus PMT_1308 in contig BX548175_GR.
KEGG	pmt:PMT1308.
NMPDR	fig|74547.1.peg.1303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	Q7TUT7.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT1308-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROMM

Accession

Primary (citable) accession number: Q7TUT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program