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Q7TUN3

- SYI_PROMM

UniProt

Q7TUN3 - SYI_PROMM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9313)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
Binding sitei628 – 6281ATPUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi958 – 9581ZincUniRule annotation
Metal bindingi961 – 9611ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PMT_1817
OrganismiProchlorococcus marinus (strain MIT 9313)
Taxonomic identifieri74547 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001423: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligasePRO_0000098440Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi74547.PMT1817.

Structurei

3D structure databases

ProteinModelPortaliQ7TUN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TUN3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQGKQPDGV ARTSYKDTLN LLQTSFGMRA NASQREPELQ EFWKQQGIDL
60 70 80 90 100
ELGLNNQGQN FTLHDGPPYA NGALHMGHAL NKVLKDIINK HQILRGRQVR
110 120 130 140 150
FVPGWDCHGL PIELKVLQNL NQEQREALTP LKLRKKAAAF ARKQVDSQMA
160 170 180 190 200
GFRRWGIWAD WEHPYLTLQK EYEAAQIQVF GKMFQKGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDGHTSP SVYVAFPAAK LPEKLRTSLG NQGLELPNNG
260 270 280 290 300
LELGQALQIA IWTTTPWTLP ANLAVSVNEK LDYSFAVDNQ GRLLLVAAEL
310 320 330 340 350
LPSLRDTLAL ELTARATVKG ALLAGLIYKH PLLDRDSPIV IGGEYITTES
360 370 380 390 400
GTGLVHTAPG HGVDDFNTGQ KHDLGMLCPV DESGTMTSEA GPFAGLNVLK
410 420 430 440 450
DANPTIIAAL EERGALLKHE PYAHRYPYDW RTKKPTIFRA TEQWFASVEG
460 470 480 490 500
FRNEALTAIN SVEWLPASGR NRMEAMVRER GDWCISRQRT WGVPIPVFYE
510 520 530 540 550
REGNEVLLND ETLAHVKALI TEHGADVWWE RNEVELLPPA YAAEAERWRK
560 570 580 590 600
GTDTMDVWFD SGSSWAAVAS QQEGLAYPTE LYLEGSDQHR GWFQSSLLTS
610 620 630 640 650
VAINSQAPYR QVLTHGFALD EKGRKMSKSL GNVVDPAVII DGGKNQKQEP
660 670 680 690 700
PYGADVLRLW VSSVDYSADV PIGASILRQI ADVYRKVRNT SRYLLGNLHD
710 720 730 740 750
FDPERDAIPV PELPLLDRWM LQRTAEVMDE ISTAFDRYEF YRFFQLLQSY
760 770 780 790 800
CVVDLSNFYL DIAKDRLYVS SPSERRRRSC QTAMALIIER LAGAISPVLC
810 820 830 840 850
HMAEDIWQNL PYSVAEDSVF RRGWPTVPET WRDPSMMAPM HQLRELRSAV
860 870 880 890 900
NRVLEDCRSQ GELGAALEAA VRLEAHSEAL QEALDWLRQQ GDPDVDGLRD
910 920 930 940 950
WLLVSHLQVG GEPWAELLAS QDNALATIEV ARARGSKCER CWHYETDVGQ
960
HTTHPTLCGR CVGVLEHQ
Length:968
Mass (Da):108,894
Last modified:October 1, 2003 - v1
Checksum:iB20E527F5FADDA19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548175 Genomic DNA. Translation: CAE21992.1.
RefSeqiNP_895644.1. NC_005071.1.
WP_011131184.1. NC_005071.1.

Genome annotation databases

EnsemblBacteriaiCAE21992; CAE21992; PMT_1817.
GeneIDi1728103.
KEGGipmt:PMT1817.
PATRICi23012413. VBIProMar135351_2334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548175 Genomic DNA. Translation: CAE21992.1 .
RefSeqi NP_895644.1. NC_005071.1.
WP_011131184.1. NC_005071.1.

3D structure databases

ProteinModelPortali Q7TUN3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 74547.PMT1817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE21992 ; CAE21992 ; PMT_1817 .
GeneIDi 1728103.
KEGGi pmt:PMT1817.
PATRICi 23012413. VBIProMar135351_2334.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9313.

Entry informationi

Entry nameiSYI_PROMM
AccessioniPrimary (citable) accession number: Q7TUN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3