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Q7TUN3

- SYI_PROMM

UniProt

Q7TUN3 - SYI_PROMM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, PMT_1817
Organism
Prochlorococcus marinus (strain MIT 9313)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylate By similarity
Binding sitei628 – 6281ATP By similarity
Metal bindingi938 – 9381Zinc By similarity
Metal bindingi941 – 9411Zinc By similarity
Metal bindingi958 – 9581Zinc By similarity
Metal bindingi961 – 9611Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PMT_1817
OrganismiProchlorococcus marinus (strain MIT 9313)
Taxonomic identifieri74547 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001423: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligaseUniRule annotationPRO_0000098440Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi74547.PMT1817.

Structurei

3D structure databases

ProteinModelPortaliQ7TUN3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotationAdd
BLAST
Motifi625 – 6295"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TUN3-1 [UniParc]FASTAAdd to Basket

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MTQGKQPDGV ARTSYKDTLN LLQTSFGMRA NASQREPELQ EFWKQQGIDL    50
ELGLNNQGQN FTLHDGPPYA NGALHMGHAL NKVLKDIINK HQILRGRQVR 100
FVPGWDCHGL PIELKVLQNL NQEQREALTP LKLRKKAAAF ARKQVDSQMA 150
GFRRWGIWAD WEHPYLTLQK EYEAAQIQVF GKMFQKGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPDGHTSP SVYVAFPAAK LPEKLRTSLG NQGLELPNNG 250
LELGQALQIA IWTTTPWTLP ANLAVSVNEK LDYSFAVDNQ GRLLLVAAEL 300
LPSLRDTLAL ELTARATVKG ALLAGLIYKH PLLDRDSPIV IGGEYITTES 350
GTGLVHTAPG HGVDDFNTGQ KHDLGMLCPV DESGTMTSEA GPFAGLNVLK 400
DANPTIIAAL EERGALLKHE PYAHRYPYDW RTKKPTIFRA TEQWFASVEG 450
FRNEALTAIN SVEWLPASGR NRMEAMVRER GDWCISRQRT WGVPIPVFYE 500
REGNEVLLND ETLAHVKALI TEHGADVWWE RNEVELLPPA YAAEAERWRK 550
GTDTMDVWFD SGSSWAAVAS QQEGLAYPTE LYLEGSDQHR GWFQSSLLTS 600
VAINSQAPYR QVLTHGFALD EKGRKMSKSL GNVVDPAVII DGGKNQKQEP 650
PYGADVLRLW VSSVDYSADV PIGASILRQI ADVYRKVRNT SRYLLGNLHD 700
FDPERDAIPV PELPLLDRWM LQRTAEVMDE ISTAFDRYEF YRFFQLLQSY 750
CVVDLSNFYL DIAKDRLYVS SPSERRRRSC QTAMALIIER LAGAISPVLC 800
HMAEDIWQNL PYSVAEDSVF RRGWPTVPET WRDPSMMAPM HQLRELRSAV 850
NRVLEDCRSQ GELGAALEAA VRLEAHSEAL QEALDWLRQQ GDPDVDGLRD 900
WLLVSHLQVG GEPWAELLAS QDNALATIEV ARARGSKCER CWHYETDVGQ 950
HTTHPTLCGR CVGVLEHQ 968
Length:968
Mass (Da):108,894
Last modified:October 1, 2003 - v1
Checksum:iB20E527F5FADDA19
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548175 Genomic DNA. Translation: CAE21992.1.
RefSeqiNP_895644.1. NC_005071.1.

Genome annotation databases

EnsemblBacteriaiCAE21992; CAE21992; PMT_1817.
GeneIDi1728103.
KEGGipmt:PMT1817.
PATRICi23012413. VBIProMar135351_2334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548175 Genomic DNA. Translation: CAE21992.1 .
RefSeqi NP_895644.1. NC_005071.1.

3D structure databases

ProteinModelPortali Q7TUN3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 74547.PMT1817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE21992 ; CAE21992 ; PMT_1817 .
GeneIDi 1728103.
KEGGi pmt:PMT1817.
PATRICi 23012413. VBIProMar135351_2334.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9313.

Entry informationi

Entry nameiSYI_PROMM
AccessioniPrimary (citable) accession number: Q7TUN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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