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Q7TUN3

- SYI_PROMM

UniProt

Q7TUN3 - SYI_PROMM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9313)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
    Binding sitei628 – 6281ATPUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation
    Metal bindingi958 – 9581ZincUniRule annotation
    Metal bindingi961 – 9611ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PMT_1817
    OrganismiProchlorococcus marinus (strain MIT 9313)
    Taxonomic identifieri74547 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000001423: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 968968Isoleucine--tRNA ligasePRO_0000098440Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi74547.PMT1817.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TUN3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TUN3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQGKQPDGV ARTSYKDTLN LLQTSFGMRA NASQREPELQ EFWKQQGIDL    50
    ELGLNNQGQN FTLHDGPPYA NGALHMGHAL NKVLKDIINK HQILRGRQVR 100
    FVPGWDCHGL PIELKVLQNL NQEQREALTP LKLRKKAAAF ARKQVDSQMA 150
    GFRRWGIWAD WEHPYLTLQK EYEAAQIQVF GKMFQKGYIY RGLKPVHWSP 200
    SSRTALAEAE LEYPDGHTSP SVYVAFPAAK LPEKLRTSLG NQGLELPNNG 250
    LELGQALQIA IWTTTPWTLP ANLAVSVNEK LDYSFAVDNQ GRLLLVAAEL 300
    LPSLRDTLAL ELTARATVKG ALLAGLIYKH PLLDRDSPIV IGGEYITTES 350
    GTGLVHTAPG HGVDDFNTGQ KHDLGMLCPV DESGTMTSEA GPFAGLNVLK 400
    DANPTIIAAL EERGALLKHE PYAHRYPYDW RTKKPTIFRA TEQWFASVEG 450
    FRNEALTAIN SVEWLPASGR NRMEAMVRER GDWCISRQRT WGVPIPVFYE 500
    REGNEVLLND ETLAHVKALI TEHGADVWWE RNEVELLPPA YAAEAERWRK 550
    GTDTMDVWFD SGSSWAAVAS QQEGLAYPTE LYLEGSDQHR GWFQSSLLTS 600
    VAINSQAPYR QVLTHGFALD EKGRKMSKSL GNVVDPAVII DGGKNQKQEP 650
    PYGADVLRLW VSSVDYSADV PIGASILRQI ADVYRKVRNT SRYLLGNLHD 700
    FDPERDAIPV PELPLLDRWM LQRTAEVMDE ISTAFDRYEF YRFFQLLQSY 750
    CVVDLSNFYL DIAKDRLYVS SPSERRRRSC QTAMALIIER LAGAISPVLC 800
    HMAEDIWQNL PYSVAEDSVF RRGWPTVPET WRDPSMMAPM HQLRELRSAV 850
    NRVLEDCRSQ GELGAALEAA VRLEAHSEAL QEALDWLRQQ GDPDVDGLRD 900
    WLLVSHLQVG GEPWAELLAS QDNALATIEV ARARGSKCER CWHYETDVGQ 950
    HTTHPTLCGR CVGVLEHQ 968
    Length:968
    Mass (Da):108,894
    Last modified:October 1, 2003 - v1
    Checksum:iB20E527F5FADDA19
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX548175 Genomic DNA. Translation: CAE21992.1.
    RefSeqiNP_895644.1. NC_005071.1.

    Genome annotation databases

    EnsemblBacteriaiCAE21992; CAE21992; PMT_1817.
    GeneIDi1728103.
    KEGGipmt:PMT1817.
    PATRICi23012413. VBIProMar135351_2334.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX548175 Genomic DNA. Translation: CAE21992.1 .
    RefSeqi NP_895644.1. NC_005071.1.

    3D structure databases

    ProteinModelPortali Q7TUN3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 74547.PMT1817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE21992 ; CAE21992 ; PMT_1817 .
    GeneIDi 1728103.
    KEGGi pmt:PMT1817.
    PATRICi 23012413. VBIProMar135351_2334.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MIT 9313.

    Entry informationi

    Entry nameiSYI_PROMM
    AccessioniPrimary (citable) accession number: Q7TUN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3