Q7TUK9 (GSHB_PROMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	PMT_2061

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence CAE22235.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutathione synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glutathione synthetase HAMAP MF_00162		PRO_0000197474
Regions
	Domain	120 – 304	185	ATP-grasp
	Nucleotide binding	146 – 202	57	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TUK9 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: C5D44F7E79D83F49	FASTA	308	33,316
	10 20 30 40 50 60 MRQLFVLDPL QCIQPAKDSS AALMQAAQRA SIEVWACTPA DLQARGDQLS AIAVPVVAEP 70 80 90 100 110 120 WISTGEPRSL PLTDFACIWM RKDPPVDEGY LYATHLLELA ERAGVCVLNR PAALRAWNEK 130 140 150 160 170 180 LGALRFNNLM APTLVASRVS ELAAFAREQE EVVLKPLGGR AGQGLVRVAG AAPGLEALLE 190 200 210 220 230 240 LVTDQEQLPV MVQRFLPAVI EGDKRILLVD GEPLGAVNRR PKAGDFRSNL AMGGRPEPTE 250 260 270 280 290 300 LDSRELQICA ELAPVLREQG LFFVGIDVID GLLSEINVTS PTGIREVERL KGVPLADQVI ARLLVSLG

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9313.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548175 Genomic DNA. Translation: CAE22235.1. Different initiation.
RefSeq	NP_895886.1. NC_005071.1.
3D structure databases
ProteinModelPortal	Q7TUK9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TUK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1729231.
GenomeReviews	Gene locus PMT_2061 in contig BX548175_GR.
KEGG	pmt:PMT2061.
NMPDR	fig|74547.1.peg.2053.
PATRIC	23012973. VBIProMar135351_2609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HBG624526.
PhylomeDB	Q7TUK9.
ProtClustDB	PRK05246.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT2061-MONOMER.
Family and domain databases
HAMAP	MF_00162. GSH-S. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01920.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. Glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_PROMM

Accession

Primary (citable) accession number: Q7TUK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program