Reviewed, UniProtKB/Swiss-Prot Q7TUG9 (GSHB_PROMP)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	PMM0178

Organism

Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) [Complete proteome] [HAMAP]

Taxonomic identifier

59919 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Glutathione synthetase HAMAP MF_00162		PRO_0000197475
Regions
	Domain	120 – 304	185	ATP-grasp
	Nucleotide binding	146 – 202	57	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TUG9-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 76EC5F13BDE80B24	FASTA	307	34,254
	10 20 30 40 50 60 MKFLFVVDPI KNINPLKDSS AALMQASSKK KIEVWICTPQ DLEARGDEVW ASSWRAEVCP 70 80 90 100 110 120 WVSFKENKCI PLAEFNCIWM RKDPPVNEGY LYATHLLEVA ERKGVKVINK PSSLRAWNEK 130 140 150 160 170 180 LGALRYSHLM APTIVASKVK DLINFAQINH DVVVKPLGGK GGQGVIRLTK DSPGIKAMIE 190 200 210 220 230 240 LITSQEQLPV MMQKFIPEVK EGDKRIIIVN GEPIGSINRI PQGGDFRSNL AMGGKAEKTN 250 260 270 280 290 300 LTAKEKKICT ELSQHFKDEG LFFVGIDVIN EMLSEINVTS PTGLREIETL SNKSVSDKVI EKLLEII

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX548174 Genomic DNA. Translation: CAE18637.1.
RefSeq	NP_892299.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1727216.
GenomeReviews	Gene locus PMM0178 in contig BX548174_GR.
KEGG	pmm:PMM0178.
NMPDR	fig|59919.1.peg.178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7TUG9.
OMA	Q7TUG9. VRNAPEK.
Enzyme and pathway databases
BioCyc	PMAR167540:PMM0178-MON.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_PROMP

Accession

Primary (citable) accession number: Q7TUG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)