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Q7TT50

- MRCKB_MOUSE

UniProt

Q7TT50 - MRCKB_MOUSE

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Protein
Serine/threonine-protein kinase MRCK beta
Gene
Cdc42bpb, Kiaa1124
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. Inhibited by chelerythrine chloride By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051ATP By similarityBy similarity
Active sitei200 – 2001Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 909ATP By similarityBy similarity
Zinc fingeri1026 – 107651Phorbol-ester/DAG-type
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB
  4. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. actomyosin structure organization Source: Ensembl
  3. cell migration Source: Ensembl
  4. intracellular signal transduction Source: InterPro
  5. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK beta (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase beta
DMPK-like beta
Myotonic dystrophy kinase-related CDC42-binding kinase beta
Short name:
MRCK beta
Short name:
Myotonic dystrophy protein kinase-like beta
Gene namesi
Name:Cdc42bpb
Synonyms:Kiaa1124
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:2136459. Cdc42bpb.

Subcellular locationi

Cytoplasm By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell junction By similarity
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent By similarity. Detected at the leading edge of migrating and wounded cells; this localization requires the presence of catalytically active CDC42 By similarity.

GO - Cellular componenti

  1. actomyosin Source: Ensembl
  2. cell leading edge Source: UniProtKB
  3. cell-cell junction Source: UniProtKB
  4. cytoplasm Source: UniProtKB-SubCell
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17131713Serine/threonine-protein kinase MRCK beta
PRO_0000086395Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211Phosphoserine; by autocatalysis By similarityBy similarity
Modified residuei233 – 2331Phosphoserine; by autocatalysis By similarityBy similarity
Modified residuei239 – 2391Phosphothreonine; by autocatalysis By similarityBy similarity
Modified residuei954 – 9541Phosphotyrosine1 Publication
Modified residuei1692 – 16921Phosphoserine2 Publications
Modified residuei1695 – 16951Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7TT50.
PaxDbiQ7TT50.
PRIDEiQ7TT50.

PTM databases

PhosphoSiteiQ7TT50.

Expressioni

Gene expression databases

BgeeiQ7TT50.
CleanExiMM_CDC42BPB.
GenevestigatoriQ7TT50.

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 By similarity. Interacts with TJP1; this interaction requires the presence of catalytically active CDC42. Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation By similarity.

Protein-protein interaction databases

BioGridi229972. 3 interactions.
IntActiQ7TT50. 1 interaction.
STRINGi10090.ENSMUSP00000042565.

Structurei

3D structure databases

ProteinModelPortaliQ7TT50.
SMRiQ7TT50. Positions 2-415.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 342267Protein kinaseBy similarity
Add
BLAST
Domaini343 – 41371AGC-kinase C-terminal
Add
BLAST
Domaini1096 – 1215120PH
Add
BLAST
Domaini1241 – 1515275CNH
Add
BLAST
Domaini1585 – 159814CRIB
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili434 – 649216 Reviewed prediction
Add
BLAST
Coiled coili681 – 815135 Reviewed prediction
Add
BLAST
Coiled coili878 – 93962 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117511.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ7TT50.
KOiK16307.
OMAiSCFSDRG.
OrthoDBiEOG7F511X.
TreeFamiTF313551.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR001849. PH_domain.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TT50-1 [UniParc]FASTAAdd to Basket

« Hide

MSAKVRLKKL EQLLLDGPWR NDSALSVETL LDVLVCLYTE CSHSALRRDK     50
YVAEFLEWAK PFTQLVKDMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER 100
IYAMKILNKW EMLKRAETAC FREERDVLVN GDCQWITALH YAFQDENYLY 150
LVMDYYVGGD LLTLLSKFED KLPEDMARFY IGEMVLAIDS IHQLHYVHRD 200
IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP DYISPEILQA 250
MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF 300
QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI 350
RNLEAPYIPD VSSPSDTSNF DVDDDMLRNI EILPPGSHTG FSGLHLPFIG 400
FTFTTESCFS DRGSLKSMTQ SNTLTKDEDV QRDLENSLQI EAYERRIRRL 450
EQEKLELSRK LQESTQTVQS LHGSTRALGN SNRDKEIKRL NEELERMKSK 500
MADSNRLERQ LEDTVTLRQE HEDSTHRLKG LEKQYRLARQ EKEELHKQLV 550
EASERLKSQT KELKDAHQQR KRALQEFSEL NERMSELRSL KQKVSRQLRD 600
KEEEMEVAMQ KIDSMRQDLR KSEKSRKELE ARLEDAAAEA SKERKLREHS 650
ESFCKQMERE LEALKVKQGG RGPGAASEHQ QEISKIRSEL EKKVLFYEEE 700
LVRREASHVL EVKNVKKEVH DSESHQLALQ KEVLMLKDKL EKSKRERHSE 750
MEEAIGTVKD KYERERAMLF DENKKLTAEN EKLCSFVDKL TAQNRQLEDE 800
LQDLASKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS KMTEELETLR 850
SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV 900
KDSSLAFESK LKESEAKNRE LLEEMQSLRK RMEEKFRADT GLKLPDFQDS 950
IFEYFNTAPL AHDLTFRTSS ASDQETQASK MDLSPSVSVA TSTEQQEDMA 1000
RPQQRPSPVP LPSTQALAMA GPKPKAHQFS IKSFPSPTQC SHCTSLMVGL 1050
IRQGYACEVC AFSCHVSCKD SAPQVCPIPP EQSKRPLGVD VQRGIGTAYK 1100
GYVKVPKPTG VKKGWQRAYA VVCDCKLFLY DLPEGKSTQP GVVASQVLDL 1150
RDEEFAVSSV LASDVIHATR RDIPCIFRVT ASLLGSPSKT SSLLILTENE 1200
NEKRKWVGIL EGLQAILHKN RLKSQVVHVA QEAYDSSLPL IKAVLAAAIV 1250
DGDRIAVGLE EGLYVIELTR DVIVRAADCK KVYQIELAPK EKIAILLCGR 1300
NHHVHLYPWS SFDGAEASNF DIKLPETKGC QLIATGTLRK SSSTCLFVAV 1350
KRLILCYEIQ RTKPFHRKFS ELVAPGHVQW MAVFKDRLCV GYPSGFSLLS 1400
IQGDGPPLDL VNPTDPSLAF LSQQSFDALC AVELKSEEYL LCFSHMGLYV 1450
DPQGRRSRMQ ELMWPAAPVA CSCSPTHVTV YSEYGVDVFD VRTMEWVQTI 1500
GLRRIRPLNS DGSLNLLGCE PPRLIYFKNK FSGTILNVPD TSDNSKKQML 1550
RTRSKRRFVF KVPEEERLQQ RREMLRDPEL RSKMISNPTN FNHVAHMGPG 1600
DGMQVLMDLP LSAAPTVQEE KQGPTPAGLP RQPPSRSKPY VSWPSSGGSE 1650
PGVPVPLRSM SDPDQDFDKE PDSDSTKHST PSNSSNPSGP PSPNSPHRSQ 1700
LPMEGLDQPS CDA 1713
Length:1,713
Mass (Da):194,753
Last modified:July 27, 2011 - v2
Checksum:iCD809A3743986A4D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961K → R in AAP34402. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277589 mRNA. Translation: AAP34402.1.
AC127580 Genomic DNA. No translation available.
AK173103 mRNA. Translation: BAD32381.1.
BC049921 mRNA. Translation: AAH49921.1.
CCDSiCCDS26176.1.
RefSeqiNP_898837.2. NM_183016.2.
UniGeneiMm.27397.

Genome annotation databases

EnsembliENSMUST00000041965; ENSMUSP00000042565; ENSMUSG00000021279.
GeneIDi217866.
KEGGimmu:217866.
UCSCiuc007pct.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277589 mRNA. Translation: AAP34402.1 .
AC127580 Genomic DNA. No translation available.
AK173103 mRNA. Translation: BAD32381.1 .
BC049921 mRNA. Translation: AAH49921.1 .
CCDSi CCDS26176.1.
RefSeqi NP_898837.2. NM_183016.2.
UniGenei Mm.27397.

3D structure databases

ProteinModelPortali Q7TT50.
SMRi Q7TT50. Positions 2-415.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229972. 3 interactions.
IntActi Q7TT50. 1 interaction.
STRINGi 10090.ENSMUSP00000042565.

PTM databases

PhosphoSitei Q7TT50.

Proteomic databases

MaxQBi Q7TT50.
PaxDbi Q7TT50.
PRIDEi Q7TT50.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041965 ; ENSMUSP00000042565 ; ENSMUSG00000021279 .
GeneIDi 217866.
KEGGi mmu:217866.
UCSCi uc007pct.2. mouse.

Organism-specific databases

CTDi 9578.
MGIi MGI:2136459. Cdc42bpb.
Rougei Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117511.
HOGENOMi HOG000294133.
HOVERGENi HBG055933.
InParanoidi Q7TT50.
KOi K16307.
OMAi SCFSDRG.
OrthoDBi EOG7F511X.
TreeFami TF313551.

Miscellaneous databases

NextBioi 376071.
PROi Q7TT50.
SOURCEi Search...

Gene expression databases

Bgeei Q7TT50.
CleanExi MM_CDC42BPB.
Genevestigatori Q7TT50.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR001849. PH_domain.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR00008. DAGPEDOMAIN.
ProDomi PD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Huang C.Q., Wu S.L., Cheng Z.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 628-1713.
    Tissue: Fetal brain.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 633-642, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1139-1713.
    Strain: FVB/N.
    Tissue: Kidney.
  6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-954, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692 AND SER-1695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMRCKB_MOUSE
AccessioniPrimary (citable) accession number: Q7TT50
Secondary accession number(s): E9QK15, Q69ZR5, Q80W33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi