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Q7TT50

- MRCKB_MOUSE

UniProt

Q7TT50 - MRCKB_MOUSE

Protein

Serine/threonine-protein kinase MRCK beta

Gene

Cdc42bpb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. Inhibited by chelerythrine chloride By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051ATPBy similarityPROSITE-ProRule annotation
    Active sitei200 – 2001Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 909ATPBy similarityPROSITE-ProRule annotation
    Zinc fingeri1026 – 107651Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB
    4. small GTPase regulator activity Source: InterPro

    GO - Biological processi

    1. actin cytoskeleton reorganization Source: UniProtKB
    2. actomyosin structure organization Source: Ensembl
    3. cell migration Source: Ensembl
    4. intracellular signal transduction Source: InterPro
    5. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase MRCK beta (EC:2.7.11.1)
    Alternative name(s):
    CDC42-binding protein kinase beta
    DMPK-like beta
    Myotonic dystrophy kinase-related CDC42-binding kinase beta
    Short name:
    MRCK beta
    Short name:
    Myotonic dystrophy protein kinase-like beta
    Gene namesi
    Name:Cdc42bpbImported
    Synonyms:Kiaa1124Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2136459. Cdc42bpb.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell junction By similarity
    Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent By similarity. Detected at the leading edge of migrating and wounded cells; this localization requires the presence of catalytically active CDC42 By similarity.By similarity

    GO - Cellular componenti

    1. actomyosin Source: Ensembl
    2. cell-cell junction Source: UniProtKB
    3. cell leading edge Source: UniProtKB
    4. cytoplasm Source: UniProtKB-SubCell
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17131713Serine/threonine-protein kinase MRCK betaPRO_0000086395Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei221 – 2211Phosphoserine; by autocatalysisBy similarity
    Modified residuei233 – 2331Phosphoserine; by autocatalysisBy similarity
    Modified residuei239 – 2391Phosphothreonine; by autocatalysisBy similarity
    Modified residuei954 – 9541Phosphotyrosine1 Publication
    Modified residuei1692 – 16921Phosphoserine2 Publications
    Modified residuei1695 – 16951Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ7TT50.
    PaxDbiQ7TT50.
    PRIDEiQ7TT50.

    PTM databases

    PhosphoSiteiQ7TT50.

    Expressioni

    Gene expression databases

    BgeeiQ7TT50.
    CleanExiMM_CDC42BPB.
    GenevestigatoriQ7TT50.

    Interactioni

    Subunit structurei

    Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 By similarity. Interacts with TJP1; this interaction requires the presence of catalytically active CDC42. Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi229972. 3 interactions.
    IntActiQ7TT50. 1 interaction.
    STRINGi10090.ENSMUSP00000042565.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TT50.
    SMRiQ7TT50. Positions 2-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini76 – 342267Protein kinaseBy similarityPROSITE-ProRule annotationAdd
    BLAST
    Domaini343 – 41371AGC-kinase C-terminalAdd
    BLAST
    Domaini1096 – 1215120PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1241 – 1515275CNHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1585 – 159814CRIBPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili434 – 649216Sequence AnalysisAdd
    BLAST
    Coiled coili681 – 815135Sequence AnalysisAdd
    BLAST
    Coiled coili878 – 93962Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 CNH domain.PROSITE-ProRule annotation
    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1026 – 107651Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117511.
    HOGENOMiHOG000294133.
    HOVERGENiHBG055933.
    InParanoidiQ7TT50.
    KOiK16307.
    OMAiSCFSDRG.
    OrthoDBiEOG7F511X.
    TreeFamiTF313551.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR001180. Citron.
    IPR000095. CRIB_dom.
    IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR014930. Myotonic_dystrophy_kinase_coil.
    IPR001849. PH_domain.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00780. CNH. 1 hit.
    PF08826. DMPK_coil. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR00008. DAGPEDOMAIN.
    ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00109. C1. 1 hit.
    SM00036. CNH. 1 hit.
    SM00285. PBD. 1 hit.
    SM00233. PH. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50219. CNH. 1 hit.
    PS50108. CRIB. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TT50-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAKVRLKKL EQLLLDGPWR NDSALSVETL LDVLVCLYTE CSHSALRRDK     50
    YVAEFLEWAK PFTQLVKDMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER 100
    IYAMKILNKW EMLKRAETAC FREERDVLVN GDCQWITALH YAFQDENYLY 150
    LVMDYYVGGD LLTLLSKFED KLPEDMARFY IGEMVLAIDS IHQLHYVHRD 200
    IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP DYISPEILQA 250
    MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF 300
    QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI 350
    RNLEAPYIPD VSSPSDTSNF DVDDDMLRNI EILPPGSHTG FSGLHLPFIG 400
    FTFTTESCFS DRGSLKSMTQ SNTLTKDEDV QRDLENSLQI EAYERRIRRL 450
    EQEKLELSRK LQESTQTVQS LHGSTRALGN SNRDKEIKRL NEELERMKSK 500
    MADSNRLERQ LEDTVTLRQE HEDSTHRLKG LEKQYRLARQ EKEELHKQLV 550
    EASERLKSQT KELKDAHQQR KRALQEFSEL NERMSELRSL KQKVSRQLRD 600
    KEEEMEVAMQ KIDSMRQDLR KSEKSRKELE ARLEDAAAEA SKERKLREHS 650
    ESFCKQMERE LEALKVKQGG RGPGAASEHQ QEISKIRSEL EKKVLFYEEE 700
    LVRREASHVL EVKNVKKEVH DSESHQLALQ KEVLMLKDKL EKSKRERHSE 750
    MEEAIGTVKD KYERERAMLF DENKKLTAEN EKLCSFVDKL TAQNRQLEDE 800
    LQDLASKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS KMTEELETLR 850
    SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV 900
    KDSSLAFESK LKESEAKNRE LLEEMQSLRK RMEEKFRADT GLKLPDFQDS 950
    IFEYFNTAPL AHDLTFRTSS ASDQETQASK MDLSPSVSVA TSTEQQEDMA 1000
    RPQQRPSPVP LPSTQALAMA GPKPKAHQFS IKSFPSPTQC SHCTSLMVGL 1050
    IRQGYACEVC AFSCHVSCKD SAPQVCPIPP EQSKRPLGVD VQRGIGTAYK 1100
    GYVKVPKPTG VKKGWQRAYA VVCDCKLFLY DLPEGKSTQP GVVASQVLDL 1150
    RDEEFAVSSV LASDVIHATR RDIPCIFRVT ASLLGSPSKT SSLLILTENE 1200
    NEKRKWVGIL EGLQAILHKN RLKSQVVHVA QEAYDSSLPL IKAVLAAAIV 1250
    DGDRIAVGLE EGLYVIELTR DVIVRAADCK KVYQIELAPK EKIAILLCGR 1300
    NHHVHLYPWS SFDGAEASNF DIKLPETKGC QLIATGTLRK SSSTCLFVAV 1350
    KRLILCYEIQ RTKPFHRKFS ELVAPGHVQW MAVFKDRLCV GYPSGFSLLS 1400
    IQGDGPPLDL VNPTDPSLAF LSQQSFDALC AVELKSEEYL LCFSHMGLYV 1450
    DPQGRRSRMQ ELMWPAAPVA CSCSPTHVTV YSEYGVDVFD VRTMEWVQTI 1500
    GLRRIRPLNS DGSLNLLGCE PPRLIYFKNK FSGTILNVPD TSDNSKKQML 1550
    RTRSKRRFVF KVPEEERLQQ RREMLRDPEL RSKMISNPTN FNHVAHMGPG 1600
    DGMQVLMDLP LSAAPTVQEE KQGPTPAGLP RQPPSRSKPY VSWPSSGGSE 1650
    PGVPVPLRSM SDPDQDFDKE PDSDSTKHST PSNSSNPSGP PSPNSPHRSQ 1700
    LPMEGLDQPS CDA 1713
    Length:1,713
    Mass (Da):194,753
    Last modified:July 27, 2011 - v2
    Checksum:iCD809A3743986A4D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 961K → R in AAP34402. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277589 mRNA. Translation: AAP34402.1.
    AC127580 Genomic DNA. No translation available.
    AK173103 mRNA. Translation: BAD32381.1.
    BC049921 mRNA. Translation: AAH49921.1.
    CCDSiCCDS26176.1.
    RefSeqiNP_898837.2. NM_183016.2.
    UniGeneiMm.27397.

    Genome annotation databases

    EnsembliENSMUST00000041965; ENSMUSP00000042565; ENSMUSG00000021279.
    GeneIDi217866.
    KEGGimmu:217866.
    UCSCiuc007pct.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277589 mRNA. Translation: AAP34402.1 .
    AC127580 Genomic DNA. No translation available.
    AK173103 mRNA. Translation: BAD32381.1 .
    BC049921 mRNA. Translation: AAH49921.1 .
    CCDSi CCDS26176.1.
    RefSeqi NP_898837.2. NM_183016.2.
    UniGenei Mm.27397.

    3D structure databases

    ProteinModelPortali Q7TT50.
    SMRi Q7TT50. Positions 2-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229972. 3 interactions.
    IntActi Q7TT50. 1 interaction.
    STRINGi 10090.ENSMUSP00000042565.

    PTM databases

    PhosphoSitei Q7TT50.

    Proteomic databases

    MaxQBi Q7TT50.
    PaxDbi Q7TT50.
    PRIDEi Q7TT50.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041965 ; ENSMUSP00000042565 ; ENSMUSG00000021279 .
    GeneIDi 217866.
    KEGGi mmu:217866.
    UCSCi uc007pct.2. mouse.

    Organism-specific databases

    CTDi 9578.
    MGIi MGI:2136459. Cdc42bpb.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117511.
    HOGENOMi HOG000294133.
    HOVERGENi HBG055933.
    InParanoidi Q7TT50.
    KOi K16307.
    OMAi SCFSDRG.
    OrthoDBi EOG7F511X.
    TreeFami TF313551.

    Miscellaneous databases

    NextBioi 376071.
    PROi Q7TT50.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q7TT50.
    CleanExi MM_CDC42BPB.
    Genevestigatori Q7TT50.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR001180. Citron.
    IPR000095. CRIB_dom.
    IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR014930. Myotonic_dystrophy_kinase_coil.
    IPR001849. PH_domain.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00780. CNH. 1 hit.
    PF08826. DMPK_coil. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00008. DAGPEDOMAIN.
    ProDomi PD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00109. C1. 1 hit.
    SM00036. CNH. 1 hit.
    SM00285. PBD. 1 hit.
    SM00233. PH. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50219. CNH. 1 hit.
    PS50108. CRIB. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Huang C.Q., Wu S.L., Cheng Z.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 628-1713.
      Tissue: Fetal brainImported.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 633-642, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1139-1713.
      Strain: FVB/NImported.
      Tissue: KidneyImported.
    6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-954, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692 AND SER-1695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1692, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMRCKB_MOUSE
    AccessioniPrimary (citable) accession number: Q7TT50
    Secondary accession number(s): E9QK15, Q69ZR5, Q80W33
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3