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Protein

Adhesion G protein-coupled receptor A3

Gene

Adgra3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor that may have a role in planar cell polarity pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor A3
Alternative name(s):
G-protein coupled receptor 125
Gene namesi
Name:Adgra3
Synonyms:Gpr125
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1917943. Adgra3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 747ExtracellularCuratedAdd BLAST720
Transmembranei748 – 768Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini769 – 785CytoplasmicCuratedAdd BLAST17
Transmembranei786 – 806Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini807 – 815ExtracellularCurated9
Transmembranei816 – 836Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini837 – 865CytoplasmicCuratedAdd BLAST29
Transmembranei866 – 886Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini887 – 908ExtracellularCuratedAdd BLAST22
Transmembranei909 – 929Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini930 – 985CytoplasmicCuratedAdd BLAST56
Transmembranei986 – 1006Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1007 – 1013ExtracellularCurated7
Transmembranei1014 – 1034Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1035 – 1310CytoplasmicCuratedAdd BLAST276

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001290128 – 1310Adhesion G protein-coupled receptor A3Add BLAST1283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi87N-linked (GlcNAc...)Sequence analysis1
Glycosylationi148N-linked (GlcNAc...)Sequence analysis1
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi253 ↔ 313PROSITE-ProRule annotation
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi422N-linked (GlcNAc...)Sequence analysis1
Glycosylationi442N-linked (GlcNAc...)Sequence analysis1
Glycosylationi581N-linked (GlcNAc...)Sequence analysis1
Glycosylationi641N-linked (GlcNAc...)Sequence analysis1
Glycosylationi676N-linked (GlcNAc...)Sequence analysis1
Glycosylationi717N-linked (GlcNAc...)Sequence analysis1
Glycosylationi810N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ7TT36.
PaxDbiQ7TT36.
PRIDEiQ7TT36.

PTM databases

iPTMnetiQ7TT36.
PhosphoSitePlusiQ7TT36.

Expressioni

Tissue specificityi

Expressed by spermatogonial progenitor cells located within the outer cell layer of the seminiferous tubule and by multipotent adult spermatogonial-derived stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000029090.
CleanExiMM_GPR125.
GenevisibleiQ7TT36. MM.

Interactioni

Subunit structurei

Interacts (via PDZ-binding motif) with DLG1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030971.

Structurei

3D structure databases

ProteinModelPortaliQ7TT36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 70LRRNTAdd BLAST43
Repeati71 – 92LRR 1Add BLAST22
Repeati95 – 116LRR 2Add BLAST22
Repeati119 – 140LRR 3Add BLAST22
Repeati143 – 164LRR 4Add BLAST22
Domaini176 – 226LRRCTAdd BLAST51
Domaini231 – 329Ig-likeAdd BLAST99
Domaini687 – 738GPSPROSITE-ProRule annotationAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1308 – 1310PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi44 – 48Poly-Ala5
Compositional biasi1109 – 1112Poly-Ala4

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG4237. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00860000133701.
HOGENOMiHOG000112767.
HOVERGENiHBG051777.
InParanoidiQ7TT36.
KOiK08462.
OMAiPVIVCGI.
OrthoDBiEOG091G03GB.
TreeFamiTF331206.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TT36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPPPLLLL PLALLALLWG GERGAAALPA GCKHDGRARG TGRAAAAAEG
60 70 80 90 100
KVVCSSLELA QVLPPDTLPN RTVTLILSNN KISELKNGSF SGLSLLERLD
110 120 130 140 150
LRNNLISRIA PGAFWGLSSL KRLDLTNNRI GCLNADVFRG LTNLVRLNLS
160 170 180 190 200
GNLFTSLSQG TFDYLGSLRS LEFQTEYLLC DCNILWMHRW VKERNITVRD
210 220 230 240 250
TRCVYPKSLQ AQPVTGVKQE LLTCDPPLEL PSFYMTPSHR QVVFEGDSLP
260 270 280 290 300
FQCMASYIDQ DMQVLWYQDG RIVETDESQG IFVEKSMIHN CSLIASALTI
310 320 330 340 350
SNIQAGSTGN WGCHVQTKRG NNTRTVDIVV LESSAQYCPP ERVVNNKGDF
360 370 380 390 400
RWPRTLAGIT AYLQCTRNTH SSGIYPGSAQ DERKAWRRCD RGGFWADDDY
410 420 430 440 450
SRCQYANDVT RVLYMFNQMP LNLTNAVATA RQLLAYTVEA ANFSDKMDVI
460 470 480 490 500
FVAEMIEKFG RFTREEKSKE LGDVMVDVAS NIMLADERVL WLAQREAKAC
510 520 530 540 550
SRIVQCLQRI ATHRLASGAH VYSTYSPNIA LEAYVIKAAG FTGMTCSVFQ
560 570 580 590 600
KVAASDRAGL SDYGRRDPDG NLDKQLSFKC NVSSTFSSLA LKNTIMEASI
610 620 630 640 650
QLPSSLLSPK HKREARAADD ALYKLQLIAF RNGKLFPATG NSTKLADDGK
660 670 680 690 700
RRTVVTPVIL TKIDGATVDT HHIPVNVTLR RIAHGADAVA AQWDFDLLNG
710 720 730 740 750
QGGWKSDGCC ILYSDENITT IQCGSLGNYA VLMDLTGTEL YTPAASLLHP
760 770 780 790 800
VVYTTAITLL LCLLAVIISY MYHHSLIRIS LKSWHMLVNL CFHILLTCVV
810 820 830 840 850
FVGGITQTRN ASVCQAVGII LHYSTLATVL WVGVTARNIY KQVTKKAKRC
860 870 880 890 900
QDPDEPPAPP RPMLRFYLIG GGIPIIVCGI TAAANIKNYG SRPSAPYCWM
910 920 930 940 950
AWEPSLGAFY GPASFITFVN CMYFLSIFIQ LKRHPERKYE LKEPTEEQQR
960 970 980 990 1000
LAANENGEIN HQDSMSLSLI STSTLENEHS FQSQLLGASL TLLLYVILWM
1010 1020 1030 1040 1050
FGAMAVSLYY PLDLVFSFFF GATCLSFSAF MMVHHCINRE DVRLAWIMMC
1060 1070 1080 1090 1100
CPGRSSYSVQ VNVQPPNSSA TNGEAPKCTN SSAESSCTNK SASSFKNSSQ
1110 1120 1130 1140 1150
GCKLTNLQAA AAQYHSNALP VNATPQLDNS LTEHSMDNDI KMHVAPLDVQ
1160 1170 1180 1190 1200
FRTNVHPSRH HKNRSKGHRA SRLTVLREYA YDVPTSVEGS VQNGLPKSRP
1210 1220 1230 1240 1250
GSNEGHSRSR RAYLAYRERQ YNPPQQDSSD ACSTLPKSSR NVEKPVSTSS
1260 1270 1280 1290 1300
KKDAPRKPAA ADLESQQKSY GLNLAVQNGP VKSNGQEGPL LATDVTGNVR
1310
TGLWKHETTV
Length:1,310
Mass (Da):144,697
Last modified:July 27, 2011 - v3
Checksum:i688D8D44059F0142
GO

Sequence cautioni

The sequence AAH52391 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → P in AAH52391 (PubMed:15489334).Curated1
Sequence conflicti1070A → T in AAH19649 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102501 Genomic DNA. No translation available.
AC130666 Genomic DNA. No translation available.
BC019649 mRNA. Translation: AAH19649.1.
BC052391 mRNA. Translation: AAH52391.1. Different initiation.
BC058251 mRNA. Translation: AAH58251.1.
CCDSiCCDS51500.1.
RefSeqiNP_598672.1. NM_133911.1.
UniGeneiMm.272974.

Genome annotation databases

EnsembliENSMUST00000030971; ENSMUSP00000030971; ENSMUSG00000029090.
GeneIDi70693.
KEGGimmu:70693.
UCSCiuc008xjz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102501 Genomic DNA. No translation available.
AC130666 Genomic DNA. No translation available.
BC019649 mRNA. Translation: AAH19649.1.
BC052391 mRNA. Translation: AAH52391.1. Different initiation.
BC058251 mRNA. Translation: AAH58251.1.
CCDSiCCDS51500.1.
RefSeqiNP_598672.1. NM_133911.1.
UniGeneiMm.272974.

3D structure databases

ProteinModelPortaliQ7TT36.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030971.

Protein family/group databases

MEROPSiP02.950.
GPCRDBiSearch...

PTM databases

iPTMnetiQ7TT36.
PhosphoSitePlusiQ7TT36.

Proteomic databases

MaxQBiQ7TT36.
PaxDbiQ7TT36.
PRIDEiQ7TT36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030971; ENSMUSP00000030971; ENSMUSG00000029090.
GeneIDi70693.
KEGGimmu:70693.
UCSCiuc008xjz.2. mouse.

Organism-specific databases

CTDi166647.
MGIiMGI:1917943. Adgra3.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG4237. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00860000133701.
HOGENOMiHOG000112767.
HOVERGENiHBG051777.
InParanoidiQ7TT36.
KOiK08462.
OMAiPVIVCGI.
OrthoDBiEOG091G03GB.
TreeFamiTF331206.

Miscellaneous databases

PROiQ7TT36.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029090.
CleanExiMM_GPR125.
GenevisibleiQ7TT36. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRA3_MOUSE
AccessioniPrimary (citable) accession number: Q7TT36
Secondary accession number(s): E9QK56, Q6PE67, Q8VE71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Most adhesion GPCRs proteins undergo autoproteolysis at the GPS domain. ADGRA3 is predicted non-cleavable because of the lack of a consensus catalytic triad sequence within GPS domain.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.