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Protein

Transcription factor SPT20 homolog

Gene

Supt20h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation-induced ATG9A trafficking during autophagy.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Gastrulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SPT20 homolog
Alternative name(s):
p38-interacting protein
Short name:
p38IP
Gene namesi
Name:Supt20h
Synonyms:D3Ertd300e, Fam48a, Supt20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1929651. Supt20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mice show gastrulation defects in which mesoderm migration is defective due to deficiency in E-cadherin protein down-regulation in the primitive streak. A weaker mutation named droopy eye (drey), which affects splicing causes incompletely penetrant defects in neural tube closure, eye development and gastrulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Transcription factor SPT20 homologPRO_0000187040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961PhosphoserineCombined sources
Modified residuei491 – 4911PhosphothreonineBy similarity
Modified residuei515 – 5151PhosphoserineBy similarity
Modified residuei520 – 5201PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TT00.
MaxQBiQ7TT00.
PaxDbiQ7TT00.
PeptideAtlasiQ7TT00.
PRIDEiQ7TT00.

PTM databases

iPTMnetiQ7TT00.
PhosphoSiteiQ7TT00.

Expressioni

Tissue specificityi

Ubiquitously expressed throughout development.1 Publication

Gene expression databases

BgeeiQ7TT00.
CleanExiMM_D3ERTD300E.
ExpressionAtlasiQ7TT00. baseline and differential.
GenevisibleiQ7TT00. MM.

Interactioni

Subunit structurei

Interacts with ATG9A (By similarity). Interacts with MAPK14.By similarity1 Publication

Protein-protein interaction databases

BioGridi208175. 1 interaction.
IntActiQ7TT00. 1 interaction.
STRINGi10090.ENSMUSP00000131454.

Family & Domainsi

Sequence similaritiesi

Belongs to the SPT20 family.Curated

Phylogenomic databases

eggNOGiENOG410IJ9A. Eukaryota.
ENOG410YRYS. LUCA.
GeneTreeiENSGT00390000013549.
HOGENOMiHOG000168361.
HOVERGENiHBG081509.
InParanoidiQ7TT00.
OrthoDBiEOG71CFKK.
PhylomeDBiQ7TT00.
TreeFamiTF329155.

Family and domain databases

InterProiIPR021950. Spt20.
[Graphical view]
PANTHERiPTHR13526. PTHR13526. 1 hit.
PfamiPF12090. Spt20. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TT00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQAVEQALD CAEYIVESAQ QRPPKRKYLS SGRKSIFQKL YDLYVEECEK
60 70 80 90 100
EPEVKKLRRN VNLLEKLVMQ ETLSCLVVNL YPGNEGYSLM LRGKNGSDSE
110 120 130 140 150
TIRLPYEEGE LLEYLDAEEL PPILVDLLEK SQVNIFHCGC VIAEIRDYRQ
160 170 180 190 200
SSNMKSPGYQ SRHILLRPTM QTLVCDVHSI TSDNHKWTQE DKLLLESQLI
210 220 230 240 250
LATAEPLCLD PSVAVACTAN RLLYNRQKMN TRPMKRCWKR YSRSSLNRQQ
260 270 280 290 300
DLSHGPPPPQ LRLLDFLQKR KERKAGQHYD LKISKAGNCV DMWKRSPCNL
310 320 330 340 350
AVPSEVDVEK YAKVEESIKS DDSQPTMWPA HDVKDDYVFE CEGGNQYQKT
360 370 380 390 400
KLTILQSLGD PLYYGKIQPW KADEENDSQM SPSHSSADDH SNWFVIGSKT
410 420 430 440 450
DAERVVNQYQ ELVQNEAKCP VKMSHSSSGS AALSPGEEAE QAETSSIQSS
460 470 480 490 500
VLGKGVKHRP PPIKLPSGSG NSSSGNYFTA QQASSFLKSP TPPPSCKPSL
510 520 530
SRKSSVDLSQ VSMLSPAALS PASSSQRHES
Length:530
Mass (Da):59,519
Last modified:October 1, 2003 - v1
Checksum:i44CCC752310C0F5D
GO
Isoform 2 (identifier: Q7TT00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-55: K → KQ

Note: No experimental confirmation available.
Show »
Length:531
Mass (Da):59,647
Checksum:i0BAF3C6A0FE9EC2A
GO
Isoform 3 (identifier: Q7TT00-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-384: H → Q
     385-530: Missing.

Note: No experimental confirmation available.
Show »
Length:384
Mass (Da):44,269
Checksum:iC60814A0151DD636
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei55 – 551K → KQ in isoform 2. 1 PublicationVSP_014880
Alternative sequencei384 – 3841H → Q in isoform 3. 1 PublicationVSP_014881
Alternative sequencei385 – 530146Missing in isoform 3. 1 PublicationVSP_014882Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139179 mRNA. Translation: AAF61447.1.
AK050619 mRNA. Translation: BAC34346.1.
AK083379 mRNA. Translation: BAC38893.1.
BC052702 mRNA. Translation: AAH52702.1.
CCDSiCCDS38431.1. [Q7TT00-2]
RefSeqiNP_064379.1. NM_019995.3. [Q7TT00-2]
XP_006501879.1. XM_006501816.2. [Q7TT00-2]
UniGeneiMm.486375.

Genome annotation databases

EnsembliENSMUST00000170552; ENSMUSP00000131454; ENSMUSG00000027751. [Q7TT00-2]
ENSMUST00000200439; ENSMUSP00000143059; ENSMUSG00000027751. [Q7TT00-1]
GeneIDi56790.
KEGGimmu:56790.
UCSCiuc008pfk.1. mouse. [Q7TT00-3]
uc008pfl.1. mouse. [Q7TT00-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139179 mRNA. Translation: AAF61447.1.
AK050619 mRNA. Translation: BAC34346.1.
AK083379 mRNA. Translation: BAC38893.1.
BC052702 mRNA. Translation: AAH52702.1.
CCDSiCCDS38431.1. [Q7TT00-2]
RefSeqiNP_064379.1. NM_019995.3. [Q7TT00-2]
XP_006501879.1. XM_006501816.2. [Q7TT00-2]
UniGeneiMm.486375.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208175. 1 interaction.
IntActiQ7TT00. 1 interaction.
STRINGi10090.ENSMUSP00000131454.

PTM databases

iPTMnetiQ7TT00.
PhosphoSiteiQ7TT00.

Proteomic databases

EPDiQ7TT00.
MaxQBiQ7TT00.
PaxDbiQ7TT00.
PeptideAtlasiQ7TT00.
PRIDEiQ7TT00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170552; ENSMUSP00000131454; ENSMUSG00000027751. [Q7TT00-2]
ENSMUST00000200439; ENSMUSP00000143059; ENSMUSG00000027751. [Q7TT00-1]
GeneIDi56790.
KEGGimmu:56790.
UCSCiuc008pfk.1. mouse. [Q7TT00-3]
uc008pfl.1. mouse. [Q7TT00-2]

Organism-specific databases

CTDi56790.
MGIiMGI:1929651. Supt20.

Phylogenomic databases

eggNOGiENOG410IJ9A. Eukaryota.
ENOG410YRYS. LUCA.
GeneTreeiENSGT00390000013549.
HOGENOMiHOG000168361.
HOVERGENiHBG081509.
InParanoidiQ7TT00.
OrthoDBiEOG71CFKK.
PhylomeDBiQ7TT00.
TreeFamiTF329155.

Miscellaneous databases

PROiQ7TT00.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TT00.
CleanExiMM_D3ERTD300E.
ExpressionAtlasiQ7TT00. baseline and differential.
GenevisibleiQ7TT00. MM.

Family and domain databases

InterProiIPR021950. Spt20.
[Graphical view]
PANTHERiPTHR13526. PTHR13526. 1 hit.
PfamiPF12090. Spt20. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "p38 and a p38-interacting protein are critical for downregulation of E-cadherin during mouse gastrulation."
    Zohn I.E., Li Y., Skolnik E.Y., Anderson K.V., Han J., Niswander L.
    Cell 125:957-969(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH MAPK14.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296 AND SER-520, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiSP20H_MOUSE
AccessioniPrimary (citable) accession number: Q7TT00
Secondary accession number(s): Q8BG53, Q9JLS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 1, 2003
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.