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Q7TSY8

- SGOL2_MOUSE

UniProt

Q7TSY8 - SGOL2_MOUSE

Protein

Shugoshin-like 2

Gene

Sgol2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation. Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. female meiotic division Source: MGI
    2. male meiosis Source: MGI
    3. meiotic sister chromatid cohesion, centromeric Source: MGI
    4. positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric Source: MGI
    5. protein localization Source: MGI

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Meiosis

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shugoshin-like 2
    Alternative name(s):
    Shugoshin-2
    Short name:
    Sgo2
    Gene namesi
    Name:Sgol2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1098767. Sgol2.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromere. Chromosomecentromerekinetochore
    Note: Centromeric localization requires the presence of BUB1 and AUKRB. During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost. During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis.

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB
    2. condensed chromosome, centromeric region Source: MGI
    3. condensed chromosome kinetochore Source: UniProtKB-SubCell
    4. condensed nuclear chromosome, centromeric region Source: MGI
    5. kinetochore Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice develop normally and survive to adulthood without any apparent alteration. However, both males and females are infertile. This defect appears cytologically as complete loss of centromere cohesion at metaphase II, leading to single chromatids, and physiologically as formation of aneuploid gametes that gave rise to infertility.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11641164Shugoshin-like 2PRO_0000055440Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1042 – 10421PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ7TSY8.
    PRIDEiQ7TSY8.

    PTM databases

    PhosphoSiteiQ7TSY8.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in proliferating cells. Highly expressed in the testis and oocytes.1 Publication

    Gene expression databases

    BgeeiQ7TSY8.
    CleanExiMM_SGOL2.
    GenevestigatoriQ7TSY8.

    Interactioni

    Subunit structurei

    Interacts with PPP2CA. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGOL2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Mad2l1Q9Z1B53EBI-2552468,EBI-2552918

    Protein-protein interaction databases

    BioGridi212920. 16 interactions.
    IntActiQ7TSY8. 17 interactions.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili62 – 11352Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the shugoshin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG25773.
    GeneTreeiENSGT00390000016383.
    HOGENOMiHOG000154309.
    HOVERGENiHBG093950.
    InParanoidiQ7TSY8.
    KOiK11581.
    OMAiKVNRKTE.
    OrthoDBiEOG790G02.
    PhylomeDBiQ7TSY8.
    TreeFamiTF350100.

    Family and domain databases

    InterProiIPR026706. SGOL2.
    [Graphical view]
    PANTHERiPTHR21577:SF2. PTHR21577:SF2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7TSY8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEYPGIKVDT VTSGIQRRVK GRIAKTNLNV SLASKIKAKI LNNSSIFKIS     50
    LKHNNRALAR ALSKEKENSR RITTEKMQLQ KEVEKLNFEN TFLRLKLNTL 100
    NKKLVEIESH VSNDLLTAIE ISSLSEFHQG SFLLSATKKQ RNSKQCKPAH 150
    LPYARVLLTS ENDDDDGADD KWQTKCNNRT ISKTSPDSTS SVSRQPSSLH 200
    QCNLKAFPPK EDNQKTCGSG HLEHTSSVDI LPNESHSDQS PKSSLSEMKT 250
    APSPSLRREK LSHGNVTMRK KCVSSTPDIL YVTDLDHQPT SSPGSNWNNE 300
    IHGHTNETSN NTQRNAECFL DLPSESSSEP DAKRMELVQK NTDSFHFQKT 350
    VYDAADMELT ATDIGKIVAV SKSKKNQNKK KADCRKETFR KVKGASSDKK 400
    RESSKRECKD GSEVGAEEEA DAARAERGAG VLDGRGDSEE PNCISSTEQP 450
    SQVNTQKKRT LQNSSDQENI QNTKRRQTYT TDEQEETNPF SRHSVKFLQD 500
    GKFDLCQKTL HHNLSKPSRQ TFVIRKSEKD NLFPNQEDKD TISENLEVTN 550
    EFHIDDLSIE ANENVCDHET QTMLDLKKSV SAQQNQTKIN KTKQKINRRT 600
    KIISVMSQVY EDNDKDIHVL EKDNFPFHTQ ANKETTSGNL ESSKEFESPL 650
    LFTRDNGSLR DCKTQNVLDL HKQIPDLYPD RNESQISKIP RQKVNRKTEV 700
    ISGVKCFSND QGVHCSEKDK SLLLQKDKDF PGTLKDLSEF DTPAFCNKDS 750
    AKSCDYKSEM LLGLKKHDPN MQPACQDDSK AGKKLRQKVN RKTEIISKIT 800
    QIHENDRGST HDSLNKKLCQ KVNISKIISQ MNQIYETINE DGNGFKSSIK 850
    DCEDIKSCDF GEINSNKKEN YDPIQDPCTL VKKTKRKGSC KAGSSLAGAK 900
    NRCGLQLTDS SQVQSVPLDS GLRHHPNEAD SGPGEQTNLP KMQKQSAGRS 950
    LGDAFSVSLG KEGSRPAKAV SKMTPKSKKR KLPLGCSPET HGTVEITPNT 1000
    DLAKAVDSQQ TEKENYLEKE KIAKRKPDFC TKVLKPLSET CSSNIKNSSL 1050
    DSMCKSSLPL SISSRKTLML EESSSLESTC IFQVGDAAHE KITTGTRNPH 1100
    HRTQKSTPGS RTSLVLVDTS SVSDTNPANP ENESEGQSSH PMRRKRQCVP 1150
    LNLTEPSLRS KMRR 1164
    Length:1,164
    Mass (Da):130,277
    Last modified:October 1, 2003 - v1
    Checksum:i64BCDCE5738576D6
    GO

    Sequence cautioni

    The sequence AAH23855.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti78 – 781Q → R in BAB30980. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC023855 mRNA. Translation: AAH23855.1. Sequence problems.
    BC044797 mRNA. Translation: AAH44797.1.
    BC052742 mRNA. Translation: AAH52742.1.
    AK017868 mRNA. Translation: BAB30980.1.
    AK042448 mRNA. Translation: BAC31263.1.
    CCDSiCCDS35574.1.
    RefSeqiNP_001171338.1. NM_001177867.1.
    NP_950172.1. NM_199007.2.
    UniGeneiMm.32800.

    Genome annotation databases

    EnsembliENSMUST00000027202; ENSMUSP00000027202; ENSMUSG00000026039.
    GeneIDi68549.
    KEGGimmu:68549.
    UCSCiuc007bbk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC023855 mRNA. Translation: AAH23855.1 . Sequence problems.
    BC044797 mRNA. Translation: AAH44797.1 .
    BC052742 mRNA. Translation: AAH52742.1 .
    AK017868 mRNA. Translation: BAB30980.1 .
    AK042448 mRNA. Translation: BAC31263.1 .
    CCDSi CCDS35574.1.
    RefSeqi NP_001171338.1. NM_001177867.1.
    NP_950172.1. NM_199007.2.
    UniGenei Mm.32800.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212920. 16 interactions.
    IntActi Q7TSY8. 17 interactions.

    PTM databases

    PhosphoSitei Q7TSY8.

    Proteomic databases

    PaxDbi Q7TSY8.
    PRIDEi Q7TSY8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027202 ; ENSMUSP00000027202 ; ENSMUSG00000026039 .
    GeneIDi 68549.
    KEGGi mmu:68549.
    UCSCi uc007bbk.2. mouse.

    Organism-specific databases

    CTDi 151246.
    MGIi MGI:1098767. Sgol2.

    Phylogenomic databases

    eggNOGi NOG25773.
    GeneTreei ENSGT00390000016383.
    HOGENOMi HOG000154309.
    HOVERGENi HBG093950.
    InParanoidi Q7TSY8.
    KOi K11581.
    OMAi KVNRKTE.
    OrthoDBi EOG790G02.
    PhylomeDBi Q7TSY8.
    TreeFami TF350100.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 327428.
    PROi Q7TSY8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q7TSY8.
    CleanExi MM_SGOL2.
    Genevestigatori Q7TSY8.

    Family and domain databases

    InterProi IPR026706. SGOL2.
    [Graphical view ]
    PANTHERi PTHR21577:SF2. PTHR21577:SF2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Mammary tumor.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-593.
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 95-103; 259-269; 579-588 AND 1056-1065, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    4. "Mammalian SGO2 appears at the inner centromere domain and redistributes depending on tension across centromeres during meiosis II and mitosis."
      Gomez R., Valdeolmillos A., Parra M.T., Viera A., Carreiro C., Roncal F., Rufas J.S., Barbero J.L., Suja J.A.
      EMBO Rep. 8:173-180(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    6. "Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells."
      Lee J., Kitajima T.S., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
      Nat. Cell Biol. 10:42-52(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSGOL2_MOUSE
    AccessioniPrimary (citable) accession number: Q7TSY8
    Secondary accession number(s): Q811I4
    , Q8C9C4, Q8CGJ4, Q9CS55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3