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Q7TSY8 (SGOL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shugoshin-like 2
Alternative name(s):
Shugoshin-2
Short name=Sgo2
Gene names
Name:Sgol2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1164 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation. Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments. Ref.5 Ref.6

Subunit structure

Interacts with PPP2CA. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGOL2 By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromere. Chromosomecentromerekinetochore. Note: Centromeric localization requires the presence of BUB1 and AUKRB. During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost. During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis. Ref.4 Ref.6

Tissue specificity

Ubiquitously expressed in proliferating cells. Highly expressed in the testis and oocytes. Ref.6

Disruption phenotype

Mice develop normally and survive to adulthood without any apparent alteration. However, both males and females are infertile. This defect appears cytologically as complete loss of centromere cohesion at metaphase II, leading to single chromatids, and physiologically as formation of aneuploid gametes that gave rise to infertility. Ref.5

Miscellaneous

Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin).

Sequence similarities

Belongs to the shugoshin family.

Sequence caution

The sequence AAH23855.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Mad2l1Q9Z1B53EBI-2552468,EBI-2552918

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11641164Shugoshin-like 2
PRO_0000055440

Regions

Coiled coil62 – 11352 Potential

Amino acid modifications

Modified residue10421Phosphoserine By similarity

Experimental info

Sequence conflict781Q → R in BAB30980. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q7TSY8 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 64BCDCE5738576D6

FASTA1,164130,277
        10         20         30         40         50         60 
MEYPGIKVDT VTSGIQRRVK GRIAKTNLNV SLASKIKAKI LNNSSIFKIS LKHNNRALAR 

        70         80         90        100        110        120 
ALSKEKENSR RITTEKMQLQ KEVEKLNFEN TFLRLKLNTL NKKLVEIESH VSNDLLTAIE 

       130        140        150        160        170        180 
ISSLSEFHQG SFLLSATKKQ RNSKQCKPAH LPYARVLLTS ENDDDDGADD KWQTKCNNRT 

       190        200        210        220        230        240 
ISKTSPDSTS SVSRQPSSLH QCNLKAFPPK EDNQKTCGSG HLEHTSSVDI LPNESHSDQS 

       250        260        270        280        290        300 
PKSSLSEMKT APSPSLRREK LSHGNVTMRK KCVSSTPDIL YVTDLDHQPT SSPGSNWNNE 

       310        320        330        340        350        360 
IHGHTNETSN NTQRNAECFL DLPSESSSEP DAKRMELVQK NTDSFHFQKT VYDAADMELT 

       370        380        390        400        410        420 
ATDIGKIVAV SKSKKNQNKK KADCRKETFR KVKGASSDKK RESSKRECKD GSEVGAEEEA 

       430        440        450        460        470        480 
DAARAERGAG VLDGRGDSEE PNCISSTEQP SQVNTQKKRT LQNSSDQENI QNTKRRQTYT 

       490        500        510        520        530        540 
TDEQEETNPF SRHSVKFLQD GKFDLCQKTL HHNLSKPSRQ TFVIRKSEKD NLFPNQEDKD 

       550        560        570        580        590        600 
TISENLEVTN EFHIDDLSIE ANENVCDHET QTMLDLKKSV SAQQNQTKIN KTKQKINRRT 

       610        620        630        640        650        660 
KIISVMSQVY EDNDKDIHVL EKDNFPFHTQ ANKETTSGNL ESSKEFESPL LFTRDNGSLR 

       670        680        690        700        710        720 
DCKTQNVLDL HKQIPDLYPD RNESQISKIP RQKVNRKTEV ISGVKCFSND QGVHCSEKDK 

       730        740        750        760        770        780 
SLLLQKDKDF PGTLKDLSEF DTPAFCNKDS AKSCDYKSEM LLGLKKHDPN MQPACQDDSK 

       790        800        810        820        830        840 
AGKKLRQKVN RKTEIISKIT QIHENDRGST HDSLNKKLCQ KVNISKIISQ MNQIYETINE 

       850        860        870        880        890        900 
DGNGFKSSIK DCEDIKSCDF GEINSNKKEN YDPIQDPCTL VKKTKRKGSC KAGSSLAGAK 

       910        920        930        940        950        960 
NRCGLQLTDS SQVQSVPLDS GLRHHPNEAD SGPGEQTNLP KMQKQSAGRS LGDAFSVSLG 

       970        980        990       1000       1010       1020 
KEGSRPAKAV SKMTPKSKKR KLPLGCSPET HGTVEITPNT DLAKAVDSQQ TEKENYLEKE 

      1030       1040       1050       1060       1070       1080 
KIAKRKPDFC TKVLKPLSET CSSNIKNSSL DSMCKSSLPL SISSRKTLML EESSSLESTC 

      1090       1100       1110       1120       1130       1140 
IFQVGDAAHE KITTGTRNPH HRTQKSTPGS RTSLVLVDTS SVSDTNPANP ENESEGQSSH 

      1150       1160 
PMRRKRQCVP LNLTEPSLRS KMRR 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-593.
Strain: C57BL/6J.
Tissue: Thymus.
[3]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 95-103; 259-269; 579-588 AND 1056-1065, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[4]"Mammalian SGO2 appears at the inner centromere domain and redistributes depending on tension across centromeres during meiosis II and mitosis."
Gomez R., Valdeolmillos A., Parra M.T., Viera A., Carreiro C., Roncal F., Rufas J.S., Barbero J.L., Suja J.A.
EMBO Rep. 8:173-180(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Shugoshin-2 is essential for the completion of meiosis but not for mitotic cell division in mice."
Llano E., Gomez R., Gutierrez-Caballero C., Herran Y., Sanchez-Martin M., Vazquez-Quinones L., Hernandez T., de Alava E., Cuadrado A., Barbero J.L., Suja J.A., Pendas A.M.
Genes Dev. 22:2400-2413(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[6]"Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells."
Lee J., Kitajima T.S., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
Nat. Cell Biol. 10:42-52(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC023855 mRNA. Translation: AAH23855.1. Sequence problems.
BC044797 mRNA. Translation: AAH44797.1.
BC052742 mRNA. Translation: AAH52742.1.
AK017868 mRNA. Translation: BAB30980.1.
AK042448 mRNA. Translation: BAC31263.1.
CCDSCCDS35574.1.
RefSeqNP_001171338.1. NM_001177867.1.
NP_950172.1. NM_199007.2.
UniGeneMm.32800.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212920. 16 interactions.
IntActQ7TSY8. 17 interactions.

PTM databases

PhosphoSiteQ7TSY8.

Proteomic databases

PaxDbQ7TSY8.
PRIDEQ7TSY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027202; ENSMUSP00000027202; ENSMUSG00000026039.
GeneID68549.
KEGGmmu:68549.
UCSCuc007bbk.2. mouse.

Organism-specific databases

CTD151246.
MGIMGI:1098767. Sgol2.

Phylogenomic databases

eggNOGNOG25773.
GeneTreeENSGT00390000016383.
HOGENOMHOG000154309.
HOVERGENHBG093950.
InParanoidQ7TSY8.
KOK11581.
OMAKVNRKTE.
OrthoDBEOG790G02.
PhylomeDBQ7TSY8.
TreeFamTF350100.

Gene expression databases

BgeeQ7TSY8.
CleanExMM_SGOL2.
GenevestigatorQ7TSY8.

Family and domain databases

InterProIPR026706. SGOL2.
[Graphical view]
PANTHERPTHR21577:SF2. PTHR21577:SF2. 1 hit.
ProtoNetSearch...

Other

NextBio327428.
PROQ7TSY8.
SOURCESearch...

Entry information

Entry nameSGOL2_MOUSE
AccessionPrimary (citable) accession number: Q7TSY8
Secondary accession number(s): Q811I4 expand/collapse secondary AC list , Q8C9C4, Q8CGJ4, Q9CS55
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot