Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CUGBP Elav-like family member 4

Gene

Celf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).By similarity

GO - Molecular functioni

  • BRE binding Source: UniProtKB
  • mRNA binding Source: MGI
  • pre-mRNA binding Source: MGI

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: Ensembl
  • mRNA splice site selection Source: MGI
  • negative regulation of excitatory postsynaptic potential Source: MGI
  • negative regulation of mRNA splicing, via spliceosome Source: MGI
  • positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of retina development in camera-type eye Source: MGI

Keywordsi

Molecular functionActivator, RNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 4
Short name:
CELF-4
Alternative name(s):
Bruno-like protein 4
CUG-BP- and ETR-3-like factor 4
RNA-binding protein BRUNOL-4
Gene namesi
Name:Celf4
Synonyms:Brul4, Brunol4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1932407 Celf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002952231 – 486CUGBP Elav-like family member 4Add BLAST486

Proteomic databases

PaxDbiQ7TSY6
PeptideAtlasiQ7TSY6
PRIDEiQ7TSY6

PTM databases

iPTMnetiQ7TSY6
PhosphoSitePlusiQ7TSY6

Expressioni

Tissue specificityi

Expressed strongly in skeletal muscle, heart and adipose tissue (at protein level). Expressed in the brain and cerebellum.2 Publications

Developmental stagei

Expressed in muscle and brain at 14 dpc (at protein level). Expressed in embryo at 7.5 dpc.2 Publications

Gene expression databases

BgeeiENSMUSG00000024268
CleanExiMM_BRUNOL4
GenevisibleiQ7TSY6 MM

Interactioni

Protein-protein interaction databases

IntActiQ7TSY6, 1 interactor
MINTiQ7TSY6
STRINGi10090.ENSMUSP00000111483

Structurei

3D structure databases

ProteinModelPortaliQ7TSY6
SMRiQ7TSY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 135RRM 1PROSITE-ProRule annotationAdd BLAST82
Domaini152 – 232RRM 2PROSITE-ProRule annotationAdd BLAST81
Domaini406 – 479RRM 3PROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 298Sufficient for RNA-binding and MSE-dependent splicing activityBy similarityAdd BLAST298
Regioni239 – 258Necessary for TNNT2 exon 5 inclusionBy similarityAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi260 – 310Ala-richAdd BLAST51

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0146 Eukaryota
ENOG410XNTW LUCA
GeneTreeiENSGT00560000076837
HOVERGENiHBG107646
InParanoidiQ7TSY6
KOiK13207
OMAiHYPVLQE
OrthoDBiEOG091G19O1
PhylomeDBiQ7TSY6
TreeFamiTF314924

Family and domain databases

CDDicd12632 RRM1_CELF3_4_5_6, 1 hit
Gene3Di3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR034648 CELF3/4/5/6_RRM1
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 3 hits
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF54928 SSF54928, 3 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSY6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD
60 70 80 90 100
HDAIKLFIGQ IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL
110 120 130 140 150
TYCERESALK AQSALHEQKT LPGMNRPIQV KPADSESRGG SSCLRQPPSQ
160 170 180 190 200
DRKLFVGMLN KQQSEDDVRR LFEAFGNIEE CTILRGPDGN SKGCAFVKYS
210 220 230 240 250
SHAEAQAAIN ALHGSQTMPG ASSSLVVKFA DTDKERTMRR MQQMAGQMGM
260 270 280 290 300
FNPMAIPFGA YGAYAQALMQ QQAALMASVA QGGYLNPMAA FAAAQMQQMA
310 320 330 340 350
ALNMNGLAAA PMTPTSGGST PPGITAPAVP SIPSPIGVNG FTGLPPQANG
360 370 380 390 400
QPAAEAVFAN GIHPYPAQSP TAADPLQQAY AGVQQYAGPA AYPAAYGQIS
410 420 430 440 450
QAFPQPPPMI PQQQREGPEG CNLLIYHLPQ EFGDAELMQM FLPFGFVSFD
460 470 480
NPASAQTAIQ AMNGFQIGMK RLKVQLKRPK DANRPY
Length:486
Mass (Da):51,932
Last modified:July 10, 2007 - v2
Checksum:i17E4648A6752CF6F
GO
Isoform 2 (identifier: Q7TSY6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-151: QD → H
     390-390: Missing.

Show »
Length:484
Mass (Da):51,755
Checksum:i1FBEEB7B1D1921EF
GO
Isoform 3 (identifier: Q7TSY6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: Missing.

Show »
Length:485
Mass (Da):51,861
Checksum:i55BDF160B69D23A8
GO
Isoform 4 (identifier: Q7TSY6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-268: Missing.
     390-390: Missing.
     417-444: Missing.

Show »
Length:456
Mass (Da):48,555
Checksum:i75D5EDB150D519E7
GO
Isoform 5 (identifier: Q7TSY6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-151: QD → H
     390-390: Missing.
     443-443: P → PFGNVISSKVFVDRATNQSKC

Show »
Length:504
Mass (Da):53,938
Checksum:iCDF2F8413FDB6AC3
GO
Isoform 6 (identifier: Q7TSY6-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-150: Missing.
     443-443: P → PFGNVISSKVFVDRATNQSKC

Show »
Length:495
Mass (Da):52,973
Checksum:i6ED5FD49578DC80F
GO
Isoform 7 (identifier: Q7TSY6-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-163: MNRPIQVKPA...LFVGMLNKQQ → VSGAGRGAAG...RDPAAQDASR
     164-486: Missing.

Show »
Length:163
Mass (Da):17,106
Checksum:iC9521282F895CCF4
GO

Sequence cautioni

The sequence BAC31762 differs from that shown. Reason: Frameshift at position 134.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352P → R in AAK00298 (PubMed:10893231).Curated1
Sequence conflicti356A → R in AAK00298 (PubMed:10893231).Curated1
Sequence conflicti395A → T in AAK00298 (PubMed:10893231).Curated1
Sequence conflicti418P → S in AAK00298 (PubMed:10893231).Curated1
Sequence conflicti448S → T in AAK00298 (PubMed:10893231).Curated1
Sequence conflicti475Q → R in BAC33334 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026833124 – 163MNRPI…LNKQQ → VSGAGRGAAGRAPTGGDFGR SGSDARGGDSRDPAAQDASR in isoform 7. 2 PublicationsAdd BLAST40
Alternative sequenceiVSP_026834140 – 150Missing in isoform 6. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_026835150 – 151QD → H in isoform 2 and isoform 5. 1 Publication2
Alternative sequenceiVSP_026836164 – 486Missing in isoform 7. 2 PublicationsAdd BLAST323
Alternative sequenceiVSP_026837268Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_026838390Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 2 Publications1
Alternative sequenceiVSP_026839417 – 444Missing in isoform 4. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_026840443P → PFGNVISSKVFVDRATNQSK C in isoform 5 and isoform 6. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515450 mRNA Translation: AAO22167.1
AK044072 mRNA Translation: BAC31762.1 Frameshift.
AK048427 mRNA Translation: BAC33334.1
AK051458 mRNA Translation: BAC34649.1
AC121094 Genomic DNA No translation available.
AC144938 Genomic DNA No translation available.
AC153142 Genomic DNA No translation available.
BC048405 mRNA Translation: AAH48405.1
BC052744 mRNA Translation: AAH52744.1
AF314173 mRNA Translation: AAK00298.1
CCDSiCCDS29106.1 [Q7TSY6-2]
CCDS50241.1 [Q7TSY6-5]
RefSeqiNP_001139764.1, NM_001146292.1 [Q7TSY6-5]
NP_001139765.1, NM_001146293.1 [Q7TSY6-3]
NP_001139766.1, NM_001146294.1 [Q7TSY6-6]
NP_001139767.1, NM_001146295.1 [Q7TSY6-7]
NP_001167545.1, NM_001174074.1 [Q7TSY6-4]
NP_573458.2, NM_133195.3 [Q7TSY6-2]
XP_006525577.1, XM_006525514.2 [Q7TSY6-1]
UniGeneiMm.266435

Genome annotation databases

EnsembliENSMUST00000025117; ENSMUSP00000025117; ENSMUSG00000024268 [Q7TSY6-6]
ENSMUST00000115816; ENSMUSP00000111483; ENSMUSG00000024268 [Q7TSY6-5]
ENSMUST00000223704; ENSMUSP00000153326; ENSMUSG00000024268 [Q7TSY6-3]
ENSMUST00000224553; ENSMUSP00000153258; ENSMUSG00000024268 [Q7TSY6-4]
ENSMUST00000225477; ENSMUSP00000153226; ENSMUSG00000024268 [Q7TSY6-2]
GeneIDi108013
KEGGimmu:108013
UCSCiuc008ehk.2 mouse [Q7TSY6-4]
uc008ehl.2 mouse [Q7TSY6-2]
uc012ban.1 mouse [Q7TSY6-3]
uc012bao.1 mouse [Q7TSY6-5]
uc012bap.1 mouse [Q7TSY6-6]
uc012baq.1 mouse [Q7TSY6-7]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCELF4_MOUSE
AccessioniPrimary (citable) accession number: Q7TSY6
Secondary accession number(s): Q80Y76
, Q811Z7, Q8BKM2, Q8BLM9, Q8BXA2, Q99PE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: March 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health