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Protein

CUGBP Elav-like family member 4

Gene

Celf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).By similarity

GO - Molecular functioni

  • BRE binding Source: UniProtKB
  • mRNA binding Source: MGI
  • nucleotide binding Source: InterPro

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: Ensembl
  • mRNA splice site selection Source: MGI
  • negative regulation of excitatory postsynaptic potential Source: MGI
  • negative regulation of mRNA splicing, via spliceosome Source: MGI
  • positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: MGI
  • regulation of retina development in camera-type eye Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 4
Short name:
CELF-4
Alternative name(s):
Bruno-like protein 4
CUG-BP- and ETR-3-like factor 4
RNA-binding protein BRUNOL-4
Gene namesi
Name:Celf4
Synonyms:Brul4, Brunol4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1932407. Celf4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486CUGBP Elav-like family member 4PRO_0000295223Add
BLAST

Proteomic databases

MaxQBiQ7TSY6.
PaxDbiQ7TSY6.
PeptideAtlasiQ7TSY6.
PRIDEiQ7TSY6.

PTM databases

iPTMnetiQ7TSY6.
PhosphoSiteiQ7TSY6.

Expressioni

Tissue specificityi

Expressed strongly in skeletal muscle, heart and adipose tissue (at protein level). Expressed in the brain and cerebellum.2 Publications

Developmental stagei

Expressed in muscle and brain at 14 dpc (at protein level). Expressed in embryo at 7.5 dpc.2 Publications

Gene expression databases

BgeeiQ7TSY6.
CleanExiMM_BRUNOL4.
GenevisibleiQ7TSY6. MM.

Interactioni

Protein-protein interaction databases

IntActiQ7TSY6. 1 interaction.
MINTiMINT-4113333.
STRINGi10090.ENSMUSP00000111483.

Structurei

3D structure databases

ProteinModelPortaliQ7TSY6.
SMRiQ7TSY6. Positions 41-262, 417-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 13582RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 23281RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini406 – 47974RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 298298Sufficient for RNA-binding and MSE-dependent splicing activityBy similarityAdd
BLAST
Regioni239 – 25820Necessary for TNNT2 exon 5 inclusionBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi260 – 31051Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOVERGENiHBG107646.
InParanoidiQ7TSY6.
KOiK13207.
OMAiRINTEHT.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ7TSY6.
TreeFamiTF314924.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSY6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYIKMATLAN GQADNASLST NGLGSSPGSA GHMNGLSHSP GNPSTIPMKD
60 70 80 90 100
HDAIKLFIGQ IPRNLDEKDL KPLFEEFGKI YELTVLKDRF TGMHKGCAFL
110 120 130 140 150
TYCERESALK AQSALHEQKT LPGMNRPIQV KPADSESRGG SSCLRQPPSQ
160 170 180 190 200
DRKLFVGMLN KQQSEDDVRR LFEAFGNIEE CTILRGPDGN SKGCAFVKYS
210 220 230 240 250
SHAEAQAAIN ALHGSQTMPG ASSSLVVKFA DTDKERTMRR MQQMAGQMGM
260 270 280 290 300
FNPMAIPFGA YGAYAQALMQ QQAALMASVA QGGYLNPMAA FAAAQMQQMA
310 320 330 340 350
ALNMNGLAAA PMTPTSGGST PPGITAPAVP SIPSPIGVNG FTGLPPQANG
360 370 380 390 400
QPAAEAVFAN GIHPYPAQSP TAADPLQQAY AGVQQYAGPA AYPAAYGQIS
410 420 430 440 450
QAFPQPPPMI PQQQREGPEG CNLLIYHLPQ EFGDAELMQM FLPFGFVSFD
460 470 480
NPASAQTAIQ AMNGFQIGMK RLKVQLKRPK DANRPY
Length:486
Mass (Da):51,932
Last modified:July 10, 2007 - v2
Checksum:i17E4648A6752CF6F
GO
Isoform 2 (identifier: Q7TSY6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-151: QD → H
     390-390: Missing.

Show »
Length:484
Mass (Da):51,755
Checksum:i1FBEEB7B1D1921EF
GO
Isoform 3 (identifier: Q7TSY6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: Missing.

Show »
Length:485
Mass (Da):51,861
Checksum:i55BDF160B69D23A8
GO
Isoform 4 (identifier: Q7TSY6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-268: Missing.
     390-390: Missing.
     417-444: Missing.

Show »
Length:456
Mass (Da):48,555
Checksum:i75D5EDB150D519E7
GO
Isoform 5 (identifier: Q7TSY6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-151: QD → H
     390-390: Missing.
     443-443: P → PFGNVISSKVFVDRATNQSKC

Show »
Length:504
Mass (Da):53,938
Checksum:iCDF2F8413FDB6AC3
GO
Isoform 6 (identifier: Q7TSY6-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-150: Missing.
     443-443: P → PFGNVISSKVFVDRATNQSKC

Show »
Length:495
Mass (Da):52,973
Checksum:i6ED5FD49578DC80F
GO
Isoform 7 (identifier: Q7TSY6-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-163: MNRPIQVKPA...LFVGMLNKQQ → VSGAGRGAAG...RDPAAQDASR
     164-486: Missing.

Show »
Length:163
Mass (Da):17,106
Checksum:iC9521282F895CCF4
GO

Sequence cautioni

The sequence BAC31762.1 differs from that shown. Reason: Frameshift at position 134. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521P → R in AAK00298 (PubMed:10893231).Curated
Sequence conflicti356 – 3561A → R in AAK00298 (PubMed:10893231).Curated
Sequence conflicti395 – 3951A → T in AAK00298 (PubMed:10893231).Curated
Sequence conflicti418 – 4181P → S in AAK00298 (PubMed:10893231).Curated
Sequence conflicti448 – 4481S → T in AAK00298 (PubMed:10893231).Curated
Sequence conflicti475 – 4751Q → R in BAC33334 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei124 – 16340MNRPI…LNKQQ → VSGAGRGAAGRAPTGGDFGR SGSDARGGDSRDPAAQDASR in isoform 7. 2 PublicationsVSP_026833Add
BLAST
Alternative sequencei140 – 15011Missing in isoform 6. 1 PublicationVSP_026834Add
BLAST
Alternative sequencei150 – 1512QD → H in isoform 2 and isoform 5. 1 PublicationVSP_026835
Alternative sequencei164 – 486323Missing in isoform 7. 2 PublicationsVSP_026836Add
BLAST
Alternative sequencei268 – 2681Missing in isoform 4. 1 PublicationVSP_026837
Alternative sequencei390 – 3901Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 2 PublicationsVSP_026838
Alternative sequencei417 – 44428Missing in isoform 4. 1 PublicationVSP_026839Add
BLAST
Alternative sequencei443 – 4431P → PFGNVISSKVFVDRATNQSK C in isoform 5 and isoform 6. 1 PublicationVSP_026840

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515450 mRNA. Translation: AAO22167.1.
AK044072 mRNA. Translation: BAC31762.1. Frameshift.
AK048427 mRNA. Translation: BAC33334.1.
AK051458 mRNA. Translation: BAC34649.1.
AC121094 Genomic DNA. No translation available.
AC144938 Genomic DNA. No translation available.
AC153142 Genomic DNA. No translation available.
BC048405 mRNA. Translation: AAH48405.1.
BC052744 mRNA. Translation: AAH52744.1.
AF314173 mRNA. Translation: AAK00298.1.
CCDSiCCDS29106.1. [Q7TSY6-2]
CCDS50241.1. [Q7TSY6-5]
RefSeqiNP_001139764.1. NM_001146292.1. [Q7TSY6-5]
NP_001139765.1. NM_001146293.1. [Q7TSY6-3]
NP_001139766.1. NM_001146294.1. [Q7TSY6-6]
NP_001139767.1. NM_001146295.1. [Q7TSY6-7]
NP_001167545.1. NM_001174074.1. [Q7TSY6-4]
NP_573458.2. NM_133195.3. [Q7TSY6-2]
XP_006525577.1. XM_006525514.2. [Q7TSY6-1]
UniGeneiMm.266435.

Genome annotation databases

EnsembliENSMUST00000025117; ENSMUSP00000025117; ENSMUSG00000024268. [Q7TSY6-2]
ENSMUST00000115816; ENSMUSP00000111483; ENSMUSG00000024268. [Q7TSY6-5]
GeneIDi108013.
KEGGimmu:108013.
UCSCiuc008ehk.2. mouse. [Q7TSY6-4]
uc008ehl.2. mouse. [Q7TSY6-2]
uc012ban.1. mouse. [Q7TSY6-3]
uc012bao.1. mouse. [Q7TSY6-5]
uc012bap.1. mouse. [Q7TSY6-6]
uc012baq.1. mouse. [Q7TSY6-7]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515450 mRNA. Translation: AAO22167.1.
AK044072 mRNA. Translation: BAC31762.1. Frameshift.
AK048427 mRNA. Translation: BAC33334.1.
AK051458 mRNA. Translation: BAC34649.1.
AC121094 Genomic DNA. No translation available.
AC144938 Genomic DNA. No translation available.
AC153142 Genomic DNA. No translation available.
BC048405 mRNA. Translation: AAH48405.1.
BC052744 mRNA. Translation: AAH52744.1.
AF314173 mRNA. Translation: AAK00298.1.
CCDSiCCDS29106.1. [Q7TSY6-2]
CCDS50241.1. [Q7TSY6-5]
RefSeqiNP_001139764.1. NM_001146292.1. [Q7TSY6-5]
NP_001139765.1. NM_001146293.1. [Q7TSY6-3]
NP_001139766.1. NM_001146294.1. [Q7TSY6-6]
NP_001139767.1. NM_001146295.1. [Q7TSY6-7]
NP_001167545.1. NM_001174074.1. [Q7TSY6-4]
NP_573458.2. NM_133195.3. [Q7TSY6-2]
XP_006525577.1. XM_006525514.2. [Q7TSY6-1]
UniGeneiMm.266435.

3D structure databases

ProteinModelPortaliQ7TSY6.
SMRiQ7TSY6. Positions 41-262, 417-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TSY6. 1 interaction.
MINTiMINT-4113333.
STRINGi10090.ENSMUSP00000111483.

PTM databases

iPTMnetiQ7TSY6.
PhosphoSiteiQ7TSY6.

Proteomic databases

MaxQBiQ7TSY6.
PaxDbiQ7TSY6.
PeptideAtlasiQ7TSY6.
PRIDEiQ7TSY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025117; ENSMUSP00000025117; ENSMUSG00000024268. [Q7TSY6-2]
ENSMUST00000115816; ENSMUSP00000111483; ENSMUSG00000024268. [Q7TSY6-5]
GeneIDi108013.
KEGGimmu:108013.
UCSCiuc008ehk.2. mouse. [Q7TSY6-4]
uc008ehl.2. mouse. [Q7TSY6-2]
uc012ban.1. mouse. [Q7TSY6-3]
uc012bao.1. mouse. [Q7TSY6-5]
uc012bap.1. mouse. [Q7TSY6-6]
uc012baq.1. mouse. [Q7TSY6-7]

Organism-specific databases

CTDi56853.
MGIiMGI:1932407. Celf4.

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOVERGENiHBG107646.
InParanoidiQ7TSY6.
KOiK13207.
OMAiRINTEHT.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ7TSY6.
TreeFamiTF314924.

Miscellaneous databases

ChiTaRSiCelf4. mouse.
PROiQ7TSY6.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TSY6.
CleanExiMM_BRUNOL4.
GenevisibleiQ7TSY6. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of murine Brunol4, a new member of the elav/bruno family."
    Meins M., Schlickum S., Wilhelm C., Missbach J., Yadav S., Glaeser B., Grzmil M., Burfeind P., Laccone F.
    Cytogenet. Genome Res. 97:254-260(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 4 AND 7).
    Strain: C57BL/6J.
    Tissue: Brain cortex, Head and Spinal ganglion.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 7).
    Strain: C57BL/6J.
    Tissue: Brain and Eye.
  5. "A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator."
    Good P.J., Chen Q., Warner S.J., Herring D.C.
    J. Biol. Chem. 275:28583-28592(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 188-486 (ISOFORMS 2/3/4/5).
    Strain: C57BL/6J.
  6. "The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing."
    Ladd A.N., Charlet-B N., Cooper T.A.
    Mol. Cell. Biol. 21:1285-1296(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCELF4_MOUSE
AccessioniPrimary (citable) accession number: Q7TSY6
Secondary accession number(s): Q80Y76
, Q811Z7, Q8BKM2, Q8BLM9, Q8BXA2, Q99PE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: July 6, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.