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Protein

Phosphatidylcholine:ceramide cholinephosphotransferase 1

Gene

Sgms1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide (By similarity).By similarity

Catalytic activityi

A ceramide + a phosphatidylcholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei291 – 2911By similarity
Active sitei334 – 3341By similarity
Active sitei338 – 3381By similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell growth Source: HGNC
  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • inflammatory response Source: RGD
  • negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  • positive regulation of gene expression Source: RGD
  • sphingomyelin biosynthetic process Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Apoptosis, Lipid metabolism, Sphingolipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylcholine:ceramide cholinephosphotransferase 1 (EC:2.7.8.27)
Alternative name(s):
Protein Mob
Sphingomyelin synthase 1
Transmembrane protein 23
Gene namesi
Name:Sgms1
Synonyms:Mob, Tmem23
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi727912. Sgms1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST
Transmembranei221 – 24121HelicalSequence analysisAdd
BLAST
Transmembranei282 – 30221HelicalSequence analysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence analysisAdd
BLAST
Topological domaini331 – 41989CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Phosphatidylcholine:ceramide cholinephosphotransferase 1PRO_0000221070Add
BLAST

Proteomic databases

PaxDbiQ7TSX5.

Expressioni

Gene expression databases

ExpressionAtlasiQ7TSX5. baseline and differential.
GenevisibleiQ7TSX5. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051643.

Structurei

3D structure databases

ProteinModelPortaliQ7TSX5.
SMRiQ7TSX5. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 7664SAMPROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the sphingomyelin synthase family.Curated
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3058. Eukaryota.
ENOG410XNSC. LUCA.
GeneTreeiENSGT00390000001630.
HOGENOMiHOG000233822.
HOVERGENiHBG048216.
InParanoidiQ7TSX5.
KOiK04714.
OMAiWWYHWLC.
OrthoDBiEOG7F7W96.
PhylomeDBiQ7TSX5.
TreeFamiTF314547.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR025749. Sphingomyelin_synth-like_dom.
[Graphical view]
PfamiPF14360. PAP2_C. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TSX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSASTMKEV VYWSPKKVAD WLLENAMPEY CEPLGHFTGQ DLINLTQEDF
60 70 80 90 100
TKPPLCRVSS DNGQRLLDMI ETLKMEHHIE AHKNGHANGH LSIGVDIPNP
110 120 130 140 150
DGSFSIKIKP NGMPNGFRKE MIKIPMPEPE RSQYPMEWGK TLLAFLYALS
160 170 180 190 200
CFVLTTVMIS VVHERVPPKE VQPPLPDTFF DHFNRVQWAF SICEINGMIL
210 220 230 240 250
VGLWLFQWLL LKYKSIISRR FFCIVGTLYL YRCITMYVTT LPVPGMHFNC
260 270 280 290 300
SPKLFGDWEA QVRRIMKLIA GGGLSITGSH NMCGDYLYSG HTVMLTLTYL
310 320 330 340 350
FIKEYSPRRL WWYHWICWLL SVVGIFCILL AHDHYTVDVV VAYYITTRLF
360 370 380 390 400
WWYHTMANQQ VLKEASQMNL LARVWWYRPF QYFEKNVQGI VPRSYHWPLP
410
WPVVHLSRQV KYSRLVNDT
Length:419
Mass (Da):49,014
Last modified:February 16, 2004 - v2
Checksum:iCD1E73E61BA6F141
GO

Sequence cautioni

The sequence AAP37281.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY280961 mRNA. Translation: AAP37281.1. Different initiation.
BC081693 mRNA. Translation: AAH81693.1.
RefSeqiNP_852051.2. NM_181386.2.
UniGeneiRn.55975.

Genome annotation databases

EnsembliENSRNOT00000054761; ENSRNOP00000051643; ENSRNOG00000012536.
ENSRNOT00000087588; ENSRNOP00000075286; ENSRNOG00000012536.
GeneIDi353229.
KEGGirno:353229.
UCSCiRGD:727912. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY280961 mRNA. Translation: AAP37281.1. Different initiation.
BC081693 mRNA. Translation: AAH81693.1.
RefSeqiNP_852051.2. NM_181386.2.
UniGeneiRn.55975.

3D structure databases

ProteinModelPortaliQ7TSX5.
SMRiQ7TSX5. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051643.

Proteomic databases

PaxDbiQ7TSX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054761; ENSRNOP00000051643; ENSRNOG00000012536.
ENSRNOT00000087588; ENSRNOP00000075286; ENSRNOG00000012536.
GeneIDi353229.
KEGGirno:353229.
UCSCiRGD:727912. rat.

Organism-specific databases

CTDi259230.
RGDi727912. Sgms1.

Phylogenomic databases

eggNOGiKOG3058. Eukaryota.
ENOG410XNSC. LUCA.
GeneTreeiENSGT00390000001630.
HOGENOMiHOG000233822.
HOVERGENiHBG048216.
InParanoidiQ7TSX5.
KOiK04714.
OMAiWWYHWLC.
OrthoDBiEOG7F7W96.
PhylomeDBiQ7TSX5.
TreeFamiTF314547.

Miscellaneous databases

PROiQ7TSX5.

Gene expression databases

ExpressionAtlasiQ7TSX5. baseline and differential.
GenevisibleiQ7TSX5. RN.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR025749. Sphingomyelin_synth-like_dom.
[Graphical view]
PfamiPF14360. PAP2_C. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete cDNA sequence of a novel gene, rat mob."
    Yuan H.F., Wang X., Wang D.M., Li H.M., Feng K., Bai C.X., Zhang R., Chen L., Li Y.H., Gao Y.H., Zhen M., Yue W., Xie C., Xie X.Y., Niu L.L., Yue W., Zhang J., Cao H., Pei X.T.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiSMS1_RAT
AccessioniPrimary (citable) accession number: Q7TSX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.