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Protein

Ubiquitin-conjugating enzyme E2 Q1

Gene

Ube2q1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Involved in female fertility and embryo implantation (PubMed:23108111). May be involved in hormonal homeostasis in females (PubMed:23108111). Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation (PubMed:18511602).1 PublicationBy similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei351Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI

GO - Biological processi

  • embryo implantation Source: MGI
  • fertilization Source: MGI
  • mating behavior Source: MGI
  • prolactin secretion Source: MGI
  • reproductive system development Source: MGI
  • suckling behavior Source: MGI

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme Q1
Galactosyl transferase-associated protein1 Publication
Short name:
GTAP1 Publication
Ubiquitin carrier protein Q1
Ubiquitin-protein ligase Q1
Gene namesi
Name:Ube2q1
Synonyms:Ube2q
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917343 Ube2q1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No reproductive defects in males but females have reduced litter size due to embryo implantation failure, normal ovulation but a longer estrus cycle, reduced levels of serum prolactin at the beginning of lactation, abnormal sexual behavior and show reduced offspring care.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351C → A: Loss of ubiquitin binding. Normal dimer formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002238771 – 422Ubiquitin-conjugating enzyme E2 Q1Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

Autoubiquitinated in vitro in the presence of NEDD4L.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ7TSS2
PaxDbiQ7TSS2
PeptideAtlasiQ7TSS2
PRIDEiQ7TSS2

PTM databases

iPTMnetiQ7TSS2
PhosphoSitePlusiQ7TSS2

Expressioni

Tissue specificityi

Expressed in liver, brain, heart, spleen, lung, kidney, muscle, ovary, epididymis, testis and placenta (PubMed:23108111). Also expressed in thymus and ES cells (PubMed:18511602). Only expressed in the uterus during pregnancy (PubMed:23108111). Expressed in oocytes and during subsequent embryonic development stages (4-cell stage, blastocyst, E8.5, E.13.5, E16.5 and E18.5) (PubMed:23108111).2 Publications

Gene expression databases

BgeeiENSMUSG00000042572
CleanExiMM_UBE2Q1
GenevisibleiQ7TSS2 MM

Interactioni

Subunit structurei

Monomer and homodimer. Only the homodimer is linked to ubiquitin through thiolester activation. Interacts (via N-terminus) with B4GALT1 (via N-terminal cytoplasmic domain); the interaction is direct.1 Publication

Protein-protein interaction databases

BioGridi213860, 1 interactor
STRINGi10090.ENSMUSP00000037939

Structurei

3D structure databases

ProteinModelPortaliQ7TSS2
SMRiQ7TSS2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0897 Eukaryota
ENOG410XRET LUCA
GeneTreeiENSGT00390000018573
HOGENOMiHOG000007757
HOVERGENiHBG061368
InParanoidiQ7TSS2
KOiK10582
OMAiDSPLHND
OrthoDBiEOG091G0LGG
PhylomeDBiQ7TSS2
TreeFamiTF313338

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 2 hits
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQPQPQGQQ QPGPGQQLGV QGAAPGAGGG PGGGPGPGPC LRRELKLLES
60 70 80 90 100
IFHRGHERFR IASACLDELS CEFLLAGAGG AGAGAAPGPH LPSRGSVPGD
110 120 130 140 150
PVRIHCNITE SYPAVPPIWS VESDDPNLAA VLERLVDIKK GNTLLLQHLK
160 170 180 190 200
RIISDLCKLY NLPQHPDVEM LDQPLPAEQC TQEEVSSEDE DEEMPEDTED
210 220 230 240 250
LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD YLNGAVSGSV
260 270 280 290 300
QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH
310 320 330 340 350
NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI
360 370 380 390 400
CMELLTKQGW SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ
410 420
SYKSLVQIHE KNGWYTPPKE DG
Length:422
Mass (Da):46,173
Last modified:February 21, 2006 - v2
Checksum:i845897861B36971F
GO
Isoform 2 (identifier: Q7TSS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Note: No experimental confirmation available.
Show »
Length:253
Mass (Da):28,487
Checksum:i8FB9519FF26585CF
GO

Sequence cautioni

The sequence AAH51487 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB26217 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121V → G in AAM60815 (PubMed:18511602).Curated1
Sequence conflicti125 – 126DP → AL in AAM60815 (PubMed:18511602).Curated2
Sequence conflicti139K → R in AAM60815 (PubMed:18511602).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0172971 – 169Missing in isoform 2. 1 PublicationAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY945937 mRNA Translation: AAY23286.1
AY112699 mRNA Translation: AAM60815.1
AK009324 mRNA Translation: BAB26217.2 Different initiation.
AK146707 mRNA Translation: BAE27373.1
BC030044 mRNA Translation: AAH30044.2
BC051487 mRNA Translation: AAH51487.1 Different initiation.
BC060966 mRNA Translation: AAH60966.1
BC082275 mRNA Translation: AAH82275.1
CCDSiCCDS17517.1 [Q7TSS2-1]
RefSeqiNP_081591.3, NM_027315.4 [Q7TSS2-1]
UniGeneiMm.41438

Genome annotation databases

EnsembliENSMUST00000038356; ENSMUSP00000037939; ENSMUSG00000042572 [Q7TSS2-1]
ENSMUST00000196726; ENSMUSP00000143422; ENSMUSG00000042572 [Q7TSS2-2]
GeneIDi70093
KEGGimmu:70093
UCSCiuc008qad.2 mouse [Q7TSS2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUB2Q1_MOUSE
AccessioniPrimary (citable) accession number: Q7TSS2
Secondary accession number(s): Q29ST1
, Q3UIY3, Q6P913, Q80UT5, Q8K2T0, Q9D7E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: May 23, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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