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Protein

Ubiquitin-conjugating enzyme E2 Q1

Gene

Ube2q1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (Potential). Involved in female fertility and embryo implantation (PubMed:23108111). May be involved in hormonal homeostasis in females (Probable). Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation (PubMed:18511602).1 PublicationPROSITE-ProRule annotation2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei351 – 3511Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin conjugating enzyme activity Source: MGI

GO - Biological processi

  1. embryo implantation Source: MGI
  2. fertilization Source: MGI
  3. mating behavior Source: MGI
  4. prolactin secretion Source: MGI
  5. reproductive system development Source: MGI
  6. suckling behavior Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q1 (EC:6.3.2.19)
Alternative name(s):
Galactosyl transferase-associated protein1 Publication
Short name:
GTAP1 Publication
Ubiquitin carrier protein Q1
Ubiquitin-protein ligase Q1
Gene namesi
Name:Ube2q1
Synonyms:Ube2q
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917343. Ube2q1.

Subcellular locationi

Nucleus 1 Publication. Cell projectionfilopodium 1 Publication. Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. filopodium Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No reproductive defects in males but females have reduced litter size due to embryo implantation failure, normal ovulation but a longer estrus cycle, reduced levels of serum prolactin at the beginning of lactation, abnormal sexual behavior and show reduced offspring care.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi351 – 3511C → A: Loss of ubiquitin binding. Normal dimer formation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Ubiquitin-conjugating enzyme E2 Q1PRO_0000223877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ7TSS2.
PaxDbiQ7TSS2.
PRIDEiQ7TSS2.

PTM databases

PhosphoSiteiQ7TSS2.

Expressioni

Tissue specificityi

Expressed in liver, brain, heart, spleen, lung, kidney, muscle, ovary, epididymis, testis and placenta (PubMed:23108111). Also expressed in thymus and ES cells (PubMed:18511602). Only expressed in the uterus during pregnancy (PubMed:23108111). Expressed in oocytes and during subsequent embryonic development stages (4-cell stage, blastocyst, E8.5, E.13.5, E16.5 and E18.5) (PubMed:23108111).2 Publications

Gene expression databases

BgeeiQ7TSS2.
CleanExiMM_UBE2Q1.
ExpressionAtlasiQ7TSS2. baseline and differential.
GenevestigatoriQ7TSS2.

Interactioni

Subunit structurei

Monomer and homodimer. Only the homodimer is linked to ubiquitin through thiolester activation. Interacts (via N-terminus) with B4GALT1 (via N-terminal cytoplasmic domain); the interaction is direct.1 Publication

Protein-protein interaction databases

BioGridi213860. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ7TSS2.
SMRiQ7TSS2. Positions 249-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG320521.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ7TSS2.
KOiK10582.
OMAiDANSKNK.
OrthoDBiEOG76DTSS.
PhylomeDBiQ7TSS2.
TreeFamiTF313338.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQPQPQGQQ QPGPGQQLGV QGAAPGAGGG PGGGPGPGPC LRRELKLLES
60 70 80 90 100
IFHRGHERFR IASACLDELS CEFLLAGAGG AGAGAAPGPH LPSRGSVPGD
110 120 130 140 150
PVRIHCNITE SYPAVPPIWS VESDDPNLAA VLERLVDIKK GNTLLLQHLK
160 170 180 190 200
RIISDLCKLY NLPQHPDVEM LDQPLPAEQC TQEEVSSEDE DEEMPEDTED
210 220 230 240 250
LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD YLNGAVSGSV
260 270 280 290 300
QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH
310 320 330 340 350
NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI
360 370 380 390 400
CMELLTKQGW SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ
410 420
SYKSLVQIHE KNGWYTPPKE DG
Length:422
Mass (Da):46,173
Last modified:February 21, 2006 - v2
Checksum:i845897861B36971F
GO
Isoform 2 (identifier: Q7TSS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Note: No experimental confirmation available.

Show »
Length:253
Mass (Da):28,487
Checksum:i8FB9519FF26585CF
GO

Sequence cautioni

The sequence AAH51487.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB26217.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211V → G in AAM60815 (PubMed:18511602).Curated
Sequence conflicti125 – 1262DP → AL in AAM60815 (PubMed:18511602).Curated
Sequence conflicti139 – 1391K → R in AAM60815 (PubMed:18511602).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 169169Missing in isoform 2. 1 PublicationVSP_017297Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY945937 mRNA. Translation: AAY23286.1.
AY112699 mRNA. Translation: AAM60815.1.
AK009324 mRNA. Translation: BAB26217.2. Different initiation.
AK146707 mRNA. Translation: BAE27373.1.
BC030044 mRNA. Translation: AAH30044.2.
BC051487 mRNA. Translation: AAH51487.1. Different initiation.
BC060966 mRNA. Translation: AAH60966.1.
BC082275 mRNA. Translation: AAH82275.1.
CCDSiCCDS17517.1. [Q7TSS2-1]
RefSeqiNP_081591.3. NM_027315.4. [Q7TSS2-1]
UniGeneiMm.41438.

Genome annotation databases

EnsembliENSMUST00000038356; ENSMUSP00000037939; ENSMUSG00000042572. [Q7TSS2-1]
GeneIDi70093.
KEGGimmu:70093.
UCSCiuc008qad.2. mouse. [Q7TSS2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY945937 mRNA. Translation: AAY23286.1.
AY112699 mRNA. Translation: AAM60815.1.
AK009324 mRNA. Translation: BAB26217.2. Different initiation.
AK146707 mRNA. Translation: BAE27373.1.
BC030044 mRNA. Translation: AAH30044.2.
BC051487 mRNA. Translation: AAH51487.1. Different initiation.
BC060966 mRNA. Translation: AAH60966.1.
BC082275 mRNA. Translation: AAH82275.1.
CCDSiCCDS17517.1. [Q7TSS2-1]
RefSeqiNP_081591.3. NM_027315.4. [Q7TSS2-1]
UniGeneiMm.41438.

3D structure databases

ProteinModelPortaliQ7TSS2.
SMRiQ7TSS2. Positions 249-409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213860. 1 interaction.

PTM databases

PhosphoSiteiQ7TSS2.

Proteomic databases

MaxQBiQ7TSS2.
PaxDbiQ7TSS2.
PRIDEiQ7TSS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038356; ENSMUSP00000037939; ENSMUSG00000042572. [Q7TSS2-1]
GeneIDi70093.
KEGGimmu:70093.
UCSCiuc008qad.2. mouse. [Q7TSS2-1]

Organism-specific databases

CTDi55585.
MGIiMGI:1917343. Ube2q1.

Phylogenomic databases

eggNOGiNOG320521.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ7TSS2.
KOiK10582.
OMAiDANSKNK.
OrthoDBiEOG76DTSS.
PhylomeDBiQ7TSS2.
TreeFamiTF313338.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi330988.
PROiQ7TSS2.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TSS2.
CleanExiMM_UBE2Q1.
ExpressionAtlasiQ7TSS2. baseline and differential.
GenevestigatoriQ7TSS2.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells."
    Wassler M.J., Shur B.D., Zhou W., Geng Y.J.
    Stem Cells 26:2006-2018(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH B4GALT1, MUTAGENESIS OF CYS-351.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 169-422 (ISOFORM 1).
    Tissue: Brain, Mammary gland and Thymus.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 29-422 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Fetal heart and Tongue.
  4. "Embryo implantation failure and other reproductive defects in Ube2q1-deficient female mice."
    Grzmil P., Altmann M.E., Adham I.M., Engel U., Jarry H., Schweyer S., Wolf S., Manz J., Engel W.
    Reproduction 145:45-56(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiUB2Q1_MOUSE
AccessioniPrimary (citable) accession number: Q7TSS2
Secondary accession number(s): Q29ST1
, Q3UIY3, Q6P913, Q80UT5, Q8K2T0, Q9D7E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 21, 2006
Last modified: March 4, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.