Reviewed, UniProtKB/Swiss-Prot Q7TSS2 (UB2Q1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 Q1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase Q1
    Ubiquitin carrier protein Q1

Gene names

Name:	Ube2q1
Synonyms:	Ube2q

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TSS2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TSS2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Ubiquitin-conjugating enzyme E2 Q1		PRO_0000223877
Sites
	Active site	351	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Natural variations
	Alternative sequence	1 – 169	169	Missing in isoform 2.		VSP_017297
Experimental info
	Sequence conflict	121	1	V → G in AAM60815. Ref.1
	Sequence conflict	125 – 126	2	DP → AL in AAM60815. Ref.1
	Sequence conflict	139	1	K → R in AAM60815. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 845897861B36971F	FASTA	422	46,173
	10 20 30 40 50 60 MQQPQPQGQQ QPGPGQQLGV QGAAPGAGGG PGGGPGPGPC LRRELKLLES IFHRGHERFR 70 80 90 100 110 120 IASACLDELS CEFLLAGAGG AGAGAAPGPH LPSRGSVPGD PVRIHCNITE SYPAVPPIWS 130 140 150 160 170 180 VESDDPNLAA VLERLVDIKK GNTLLLQHLK RIISDLCKLY NLPQHPDVEM LDQPLPAEQC 190 200 210 220 230 240 TQEEVSSEDE DEEMPEDTED LDHYEMKEEE PAEGKKSEDD GIGKENLAIL EKIKKNQRQD 250 260 270 280 290 300 YLNGAVSGSV QATDRLMKEL RDIYRSQSFK GGNYAVELVN DSLYDWNVKL LKVDQDSALH 310 320 330 340 350 360 NDLQILKEKE GADFILLNFS FKDNFPFDPP FVRVVSPVLS GGYVLGGGAI CMELLTKQGW 370 380 390 400 410 420 SSAYSIESVI MQISATLVKG KARVQFGANK SQYSLTRAQQ SYKSLVQIHE KNGWYTPPKE DG
	Isoform 2. Checksum: 8FB9519FF26585CF	FASTA	253	28,487
	10 20 30 40 50 60 MLDQPLPAEQ CTQEEVSSED EDEEMPEDTE DLDHYEMKEE EPAEGKKSED DGIGKENLAI 70 80 90 100 110 120 LEKIKKNQRQ DYLNGAVSGS VQATDRLMKE LRDIYRSQSF KGGNYAVELV NDSLYDWNVK 130 140 150 160 170 180 LLKVDQDSAL HNDLQILKEK EGADFILLNF SFKDNFPFDP PFVRVVSPVL SGGYVLGGGA 190 200 210 220 230 240 ICMELLTKQG WSSAYSIESV IMQISATLVK GKARVQFGAN KSQYSLTRAQ QSYKSLVQIH 250 EKNGWYTPPK EDG

References

[1]	"Isolation and characterization of the human UBE2Q gene and its murine ortholog." Altmann M.E., Schulze E., Adham I.M., Koehler M., Engel W. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: 129/Sv.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 169-422 (ISOFORM 1). Tissue: Brain, Mammary gland and Thymus.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 29-422 (ISOFORM 1). Strain: C57BL/6J. Tissue: Fetal heart and Tongue.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY112699 mRNA. Translation: AAM60815.1. AK009324 mRNA. Translation: BAB26217.2. Different initiation. AK146707 mRNA. Translation: BAE27373.1. BC030044 mRNA. Translation: AAH30044.2. BC051487 mRNA. Translation: AAH51487.1. Different initiation. BC060966 mRNA. Translation: AAH60966.1. BC082275 mRNA. Translation: AAH82275.1.
IPI	IPI00187257. IPI00720081.
RefSeq	NP_081591.3.
UniGene	Mm.41438
3D structure databases
SMR	Q7TSS2. Positions 249-409.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TSS2.
PTM databases
PhosphoSite	Q7TSS2.
Proteomic databases
PRIDE	Q7TSS2.
Genome annotation databases
Ensembl	ENSMUST00000038356; ENSMUSP00000037939; ENSMUSG00000042572; Mus musculus. [Genome view]
GeneID	70093.
KEGG	mmu:70093.
Organism-specific databases
CTD	70093.
MGI	MGI:1917343. Ube2q1.
Phylogenomic databases
HOGENOM	HBG377833.
HOVERGEN	Q7TSS2.
InParanoid	Q7TSS2.
OMA	PLPADQC.
OrthoDB	EOG9D81R5.
PhylomeDB	Q7TSS2.
Enzyme and pathway databases
BRENDA	6.3.2.19. 244.
Gene expression databases
ArrayExpress	Q7TSS2.
Bgee	Q7TSS2.
CleanEx	MM_UBE2Q1.
Genevestigator	Q7TSS2.
GermOnline	ENSMUSG00000042572. Mus musculus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	330988.
SOURCE	Search...

Entry information

Entry name

UB2Q1_MOUSE

Accession

Primary (citable) accession number: Q7TSS2
Secondary accession number(s): Q3UIY3 , Q6P913, Q80UT5, Q8K2T0, Q9D7E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 21, 2006
Last modified:	January 19, 2010
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)