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Q7TSP5 (VTCN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-set domain containing T-cell activation inhibitor 1
Alternative name(s):
Immune costimulatory protein B7-H4
Protein B7S1
T cell costimulatory molecule B7x
Gene names
Name:Vtcn1
Synonyms:B7h4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Ref.1 Ref.2 Ref.3 UniProtKB Q7Z7D3

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Note: Expressed at the cell surface. A soluble form has also been detected. Ref.1 Ref.2 Ref.3

Tissue specificity

Expressed on the surface of professional antigen-presenting cells (at protein level). Widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. Ref.1 Ref.2 Ref.3

Induction

Down-regulated upon activation of B-cells. Ref.2

Post-translational modification

N-glycosylated By similarity. UniProtKB Q7Z7D3

Disruption phenotype

Mice mount mildy augmented T-helper 1 responses and display slightly lowered parasitic burdens upon Leishmania major infection. Ref.6

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family. UniProtKB Q5ZPR3

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 257233V-set domain containing T-cell activation inhibitor 1
PRO_0000339238
Propeptide258 – 28326Removed in mature form Potential
PRO_0000339239

Regions

Domain35 – 144110Ig-like V-type 1
Domain153 – 24189Ig-like V-type 2

Amino acid modifications

Lipidation2571GPI-anchor amidated glycine Potential
Glycosylation2161N-linked (GlcNAc...) Potential
Disulfide bond56 ↔ 130 By similarity
Disulfide bond168 ↔ 225 By similarity

Experimental info

Sequence conflict1341T → S in AAH32925. Ref.5
Sequence conflict2631F → S in AAH32925. Ref.5
Sequence conflict2681V → A in AAP88965. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q7TSP5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 7E2F174618578519

FASTA28330,875
        10         20         30         40         50         60 
MASLGQIIFW SIINIIIILA GAIALIIGFG ISGKHFITVT TFTSAGNIGE DGTLSCTFEP 

        70         80         90        100        110        120 
DIKLNGIVIQ WLKEGIKGLV HEFKEGKDDL SQQHEMFRGR TAVFADQVVV GNASLRLKNV 

       130        140        150        160        170        180 
QLTDAGTYTC YIRTSKGKGN ANLEYKTGAF SMPEINVDYN ASSESLRCEA PRWFPQPTVA 

       190        200        210        220        230        240 
WASQVDQGAN FSEVSNTSFE LNSENVTMKV VSVLYNVTIN NTYSCMIEND IAKATGDIKV 

       250        260        270        280 
TDSEVKRRSQ LQLLNSGPSP CVFSSAFVAG WALLSLSCCL MLR 

« Hide

References

« Hide 'large scale' references
[1]"B7-H4, a molecule of the B7 family, negatively regulates T cell immunity."
Sica G.L., Choi I.-H., Zhu G., Tamada K., Wang S.-D., Tamura H., Chapoval A.I., Flies D.B., Bajorath J., Chen L.
Immunity 18:849-861(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Spleen.
[2]"B7S1, a novel B7 family member that negatively regulates T cell activation."
Prasad D.V.R., Richards S., Mai X.M., Dong C.
Immunity 18:863-873(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GPI-ANCHOR.
[3]"B7x: a widely expressed B7 family member that inhibits T cell activation."
Zang X., Loke P., Kim J., Murphy K., Waitz R., Allison J.P.
Proc. Natl. Acad. Sci. U.S.A. 100:10388-10392(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: FVB/N.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Blastocyst and Oviduct.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[6]"Generation and characterization of B7-H4/B7S1/B7x-deficient mice."
Suh W.-K., Wang S., Duncan G.S., Miyazaki Y., Cates E., Walker T., Gajewska B.U., Deenick E., Dawicki W., Okada H., Wakeham A., Itie A., Watts T.H., Ohashi P.S., Jordana M., Yoshida H., Mak T.W.
Mol. Cell. Biol. 26:6403-6411(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY280973 mRNA. Translation: AAP37284.1.
AY322147 mRNA. Translation: AAP88965.1.
AY346099 mRNA. Translation: AAQ24205.1.
AK143276 mRNA. Translation: BAE25330.1.
AK145664 mRNA. Translation: BAE26576.1.
BC032925 mRNA. Translation: AAH32925.1.
RefSeqNP_848709.2. NM_178594.3.
UniGeneMm.137467.

3D structure databases

ProteinModelPortalQ7TSP5.
SMRQ7TSP5. Positions 35-243.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI43.001.

Proteomic databases

PRIDEQ7TSP5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000054791; ENSMUSP00000057721; ENSMUSG00000051076.
GeneID242122.
KEGGmmu:242122.
UCSCuc008qqx.2. mouse.

Organism-specific databases

CTD79679.
MGIMGI:3039619. Vtcn1.

Phylogenomic databases

eggNOGNOG87575.
GeneTreeENSGT00700000104325.
HOGENOMHOG000139645.
HOVERGENHBG097943.
InParanoidQ7TSP5.
KOK06747.
OMASQVDQGA.
OrthoDBEOG7FBRH9.
PhylomeDBQ7TSP5.
TreeFamTF331083.

Gene expression databases

BgeeQ7TSP5.
GenevestigatorQ7TSP5.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio385239.
PROQ7TSP5.
SOURCESearch...

Entry information

Entry nameVTCN1_MOUSE
AccessionPrimary (citable) accession number: Q7TSP5
Secondary accession number(s): Q7TPH5, Q8K091
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot