V-set domain containing T-cell activation inhibitor 1 precursor

Contribute

Send feedback

Read comments (?) or add your own

Q7TSP5 (VTCN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
V-set domain containing T-cell activation inhibitor 1

Alternative name(s):
Immune costimulatory protein B7-H4
Protein B7S1
T cell costimulatory molecule B7x

Gene names

Name:	Vtcn1
Synonyms:	B7h4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Ref.1 Ref.2 Ref.3 UniProtKB Q7Z7D3
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Note: Expressed at the cell surface. A soluble form has also been detected. Ref.1 Ref.2 Ref.3
Tissue specificity	Expressed on the surface of professional antigen-presenting cells (at protein level). Widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. Ref.1 Ref.2 Ref.3
Induction	Down-regulated upon activation of B-cells. Ref.2
Post-translational modification	N-glycosylated By similarity. UniProtKB Q7Z7D3
Disruption phenotype	Mice mount mildy augmented T-helper 1 responses and display slightly lowered parasitic burdens upon Leishmania major infection. Ref.6
Sequence similarities	Belongs to the immunoglobulin superfamily. BTN/MOG family. UniProtKB Q5ZPR3 Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Cell membrane Membrane
Domain	Immunoglobulin domain Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	interferon-gamma secretion Inferred from mutant phenotype Ref.6. Source: MGI interleukin-4 secretion Inferred from mutant phenotype Ref.6. Source: MGI negative regulation of T cell activation Inferred from direct assay PubMed 15585831. Source: MGI response to protozoan Inferred from mutant phenotype Ref.6. Source: MGI
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay PubMed 17051145. Source: MGI
Molecular_function	receptor binding Inferred from genetic interaction PubMed 15585831. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

Potential

Chain

25 – 257

233

V-set domain containing T-cell activation inhibitor 1

PRO_0000339238

Propeptide

258 – 283

Removed in mature form Potential

PRO_0000339239

Regions

Domain

35 – 144

110

Ig-like V-type 1

Domain

153 – 241

Ig-like V-type 2

Amino acid modifications

Lipidation

257

GPI-anchor amidated glycine Potential

Glycosylation

216

N-linked (GlcNAc...) Potential

Disulfide bond

56 ↔ 130

By similarity

Disulfide bond

168 ↔ 225

By similarity

Experimental info

Sequence conflict

134

T → S in AAH32925. Ref.5

Sequence conflict

263

F → S in AAH32925. Ref.5

Sequence conflict

268

V → A in AAP88965. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q7TSP5 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 7E2F174618578519
Show »

FASTA

283

30,875

        10         20         30         40         50         60 
MASLGQIIFW SIINIIIILA GAIALIIGFG ISGKHFITVT TFTSAGNIGE DGTLSCTFEP 

        70         80         90        100        110        120 
DIKLNGIVIQ WLKEGIKGLV HEFKEGKDDL SQQHEMFRGR TAVFADQVVV GNASLRLKNV 

       130        140        150        160        170        180 
QLTDAGTYTC YIRTSKGKGN ANLEYKTGAF SMPEINVDYN ASSESLRCEA PRWFPQPTVA 

       190        200        210        220        230        240 
WASQVDQGAN FSEVSNTSFE LNSENVTMKV VSVLYNVTIN NTYSCMIEND IAKATGDIKV 

       250        260        270        280 
TDSEVKRRSQ LQLLNSGPSP CVFSSAFVAG WALLSLSCCL MLR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"B7-H4, a molecule of the B7 family, negatively regulates T cell immunity." Sica G.L., Choi I.-H., Zhu G., Tamada K., Wang S.-D., Tamura H., Chapoval A.I., Flies D.B., Bajorath J., Chen L. Immunity 18:849-861(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Spleen.
[2]	"B7S1, a novel B7 family member that negatively regulates T cell activation." Prasad D.V.R., Richards S., Mai X.M., Dong C. Immunity 18:863-873(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, GPI-ANCHOR.
[3]	"B7x: a widely expressed B7 family member that inhibits T cell activation." Zang X., Loke P., Kim J., Murphy K., Waitz R., Allison J.P. Proc. Natl. Acad. Sci. U.S.A. 100:10388-10392(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: FVB/N.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Blastocyst and Oviduct.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"Generation and characterization of B7-H4/B7S1/B7x-deficient mice." Suh W.-K., Wang S., Duncan G.S., Miyazaki Y., Cates E., Walker T., Gajewska B.U., Deenick E., Dawicki W., Okada H., Wakeham A., Itie A., Watts T.H., Ohashi P.S., Jordana M., Yoshida H., Mak T.W. Mol. Cell. Biol. 26:6403-6411(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY280973 mRNA. Translation: AAP37284.1. AY322147 mRNA. Translation: AAP88965.1. AY346099 mRNA. Translation: AAQ24205.1. AK143276 mRNA. Translation: BAE25330.1. AK145664 mRNA. Translation: BAE26576.1. BC032925 mRNA. Translation: AAH32925.1.
IPI	IPI00169522.
RefSeq	NP_848709.2. NM_178594.3.
UniGene	Mm.137467.
3D structure databases
HSSP	HSSP built from PDB template 1PKO based on UniProtKB Q63345.
ProteinModelPortal	Q7TSP5.
SMR	Q7TSP5. Positions 35-243.
ModBase	Search...
Protein family/group databases
MEROPS	I43.001.
Proteomic databases
PRIDE	Q7TSP5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000054791; ENSMUSP00000057721; ENSMUSG00000051076.
GeneID	242122.
KEGG	mmu:242122.
UCSC	uc008qqx.2. mouse.
Organism-specific databases
CTD	79679.
MGI	MGI:3039619. Vtcn1.
Phylogenomic databases
eggNOG	NOG87575.
GeneTree	ENSGT00700000104325.
HOGENOM	HOG000139645.
HOVERGEN	HBG097943.
InParanoid	Q7TSP5.
KO	K06747.
OMA	MFRGRTA.
OrthoDB	EOG4MGS84.
Gene expression databases
Bgee	Q7TSP5.
Genevestigator	Q7TSP5.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	385239.
SOURCE	Search...

Entry information

Entry name

VTCN1_MOUSE

Accession

Primary (citable) accession number: Q7TSP5
Secondary accession number(s): Q7TPH5, Q8K091

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents