Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Immunoglobulin superfamily member 5

Gene

Igsf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells.2 Publications

GO - Molecular functioni

  • PDZ domain binding Source: MGI

GO - Biological processi

  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 5
Short name:
IgSF5
Alternative name(s):
Junctional adhesion molecule 4
Short name:
JAM-4
Gene namesi
Name:Igsf5
Synonyms:Jam4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919308. Igsf5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 239ExtracellularSequence analysisAdd BLAST215
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 370CytoplasmicSequence analysisAdd BLAST110

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • bicellular tight junction Source: MGI
  • cell surface Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000031629625 – 370Immunoglobulin superfamily member 5Add BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Glycosylationi45N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi46 ↔ 109PROSITE-ProRule annotation
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi149 ↔ 201PROSITE-ProRule annotation
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TSN7.
PRIDEiQ7TSN7.

PTM databases

iPTMnetiQ7TSN7.
PhosphoSitePlusiQ7TSN7.

Expressioni

Tissue specificityi

Localized to kidney glomeruli and small intestinal epithelial cells. In kidney glomeruli, it is localized at slit diaphragm. Also found in spermatogonia, gonocytes, hematopoietic stem cells and Sertoli cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000000159.
CleanExiMM_IGSF5.

Interactioni

Subunit structurei

Interacts with MAGI1 at tight junctions, forms a tripartite complex with NPHS1 (By similarity). Interacts with LNX1 isoform 2 via its PDZ 2 domain, it may also interact with other isoforms containing this domain.By similarity2 Publications

GO - Molecular functioni

  • PDZ domain binding Source: MGI

Protein-protein interaction databases

BioGridi215124. 3 interactors.
STRINGi10090.ENSMUSP00000109425.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi368 – 370Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VQGX-ray1.35B363-370[»]
ProteinModelPortaliQ7TSN7.
SMRiQ7TSN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 125Ig-like V-type 1Add BLAST101
Domaini128 – 215Ig-like V-type 2Add BLAST88

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi262 – 265Poly-Cys4

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJ9. Eukaryota.
ENOG410Y7FC. LUCA.
HOGENOMiHOG000113018.
HOVERGENiHBG059033.
InParanoidiQ7TSN7.
KOiK06786.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TSN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSWRDVLA VLVILAQLTA SGSSYQIIEG PQNVTVLKDS EAHFNCTVTH
60 70 80 90 100
GWKLLMWTLN QMVVLSLTTQ GPIITNNRFT YASYNSTDSF ISELIIHDVQ
110 120 130 140 150
PSDSGSVQCS LQNSHGFGSA FLSVQVMGTL NIPSNNLIVT EGEPCNVTCY
160 170 180 190 200
AVGWTSLPDI SWELEVPVSH SSYNSFLESG NFMRVLSVLD LTPLGNGTLT
210 220 230 240 250
CVAELKDLQA SKSLTVNLTV VQPPPDSIGE EGPALPTWAI ILLAVAFSLL
260 270 280 290 300
LILIIVLIII FCCCCASRRE KEESTYQNEI RKSANMRTNK ADPETKLKGG
310 320 330 340 350
KENYGYSSDE AKAAQTASLP PKSAEVSLPE KRSSSLPYQE LNKHQPGPAT
360 370
HPRVSFDIAS PQKVRNVTLV
Length:370
Mass (Da):40,292
Last modified:October 1, 2003 - v1
Checksum:i001B40AC535C96EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126 – 225Missing in BAB25436 (PubMed:16141072).CuratedAdd BLAST100
Sequence conflicti126 – 225Missing in AAH04806 (PubMed:16141072).CuratedAdd BLAST100
Sequence conflicti299G → S in BAB25436 (PubMed:16141072).Curated1
Sequence conflicti299G → S in AAH04806 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF537215 mRNA. Translation: AAP49218.1.
AK008060 mRNA. Translation: BAB25436.1.
BC004806 mRNA. Translation: AAH04806.1.
CCDSiCCDS49924.1.
RefSeqiNP_082354.1. NM_028078.3.
UniGeneiMm.119714.

Genome annotation databases

GeneIDi72058.
KEGGimmu:72058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF537215 mRNA. Translation: AAP49218.1.
AK008060 mRNA. Translation: BAB25436.1.
BC004806 mRNA. Translation: AAH04806.1.
CCDSiCCDS49924.1.
RefSeqiNP_082354.1. NM_028078.3.
UniGeneiMm.119714.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VQGX-ray1.35B363-370[»]
ProteinModelPortaliQ7TSN7.
SMRiQ7TSN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215124. 3 interactors.
STRINGi10090.ENSMUSP00000109425.

PTM databases

iPTMnetiQ7TSN7.
PhosphoSitePlusiQ7TSN7.

Proteomic databases

PaxDbiQ7TSN7.
PRIDEiQ7TSN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72058.
KEGGimmu:72058.

Organism-specific databases

CTDi150084.
MGIiMGI:1919308. Igsf5.

Phylogenomic databases

eggNOGiENOG410IFJ9. Eukaryota.
ENOG410Y7FC. LUCA.
HOGENOMiHOG000113018.
HOVERGENiHBG059033.
InParanoidiQ7TSN7.
KOiK06786.

Miscellaneous databases

ChiTaRSiIgsf5. mouse.
PROiQ7TSN7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000159.
CleanExiMM_IGSF5.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIGSF5_MOUSE
AccessioniPrimary (citable) accession number: Q7TSN7
Secondary accession number(s): Q9D8G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.