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Q7TSN7

- IGSF5_MOUSE

UniProt

Q7TSN7 - IGSF5_MOUSE

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Protein
Immunoglobulin superfamily member 5
Gene
Igsf5, Jam4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells.2 Publications

GO - Molecular functioni

  1. PDZ domain binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin superfamily member 5
Short name:
IgSF5
Alternative name(s):
Junctional adhesion molecule 4
Short name:
JAM-4
Gene namesi
Name:Igsf5
Synonyms:Jam4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1919308. Igsf5.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein. Cell junctiontight junction
Note: In kidney glomeruli, it is localized at slit diaphragm.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 239215Extracellular Reviewed prediction
Add
BLAST
Transmembranei240 – 26021Helical; Reviewed prediction
Add
BLAST
Topological domaini261 – 370110Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
  4. tight junction Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 370346Immunoglobulin superfamily member 5
PRO_0000316296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi45 – 451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi46 ↔ 109 By similarity
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi149 ↔ 201 By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi217 – 2171N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TSN7.
PRIDEiQ7TSN7.

PTM databases

PhosphoSiteiQ7TSN7.

Expressioni

Tissue specificityi

Localized to kidney glomeruli and small intestinal epithelial cells. Also found in spermatogonia, gonocytes, hematopoietic stem cells and Sertoli cells.2 Publications

Gene expression databases

ArrayExpressiQ7TSN7.
BgeeiQ7TSN7.
CleanExiMM_IGSF5.
GenevestigatoriQ7TSN7.

Interactioni

Subunit structurei

Interacts with MAGI1 at tight junctions, forms a tripartite complex with NPHS1 By similarity. Interacts with LNX1 isoform 2 via its PDZ 2 domain, it may also interact with other isoforms containing this domain.2 Publications

Protein-protein interaction databases

BioGridi215124. 3 interactions.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi368 – 3703

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VQGX-ray1.35B363-370[»]
ProteinModelPortaliQ7TSN7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 125101Ig-like V-type 1
Add
BLAST
Domaini128 – 21588Ig-like V-type 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi262 – 2654Poly-Cys

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47258.
GeneTreeiENSGT00390000018711.
HOGENOMiHOG000113018.
HOVERGENiHBG059033.
KOiK06786.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TSN7-1 [UniParc]FASTAAdd to Basket

« Hide

MEGSWRDVLA VLVILAQLTA SGSSYQIIEG PQNVTVLKDS EAHFNCTVTH    50
GWKLLMWTLN QMVVLSLTTQ GPIITNNRFT YASYNSTDSF ISELIIHDVQ 100
PSDSGSVQCS LQNSHGFGSA FLSVQVMGTL NIPSNNLIVT EGEPCNVTCY 150
AVGWTSLPDI SWELEVPVSH SSYNSFLESG NFMRVLSVLD LTPLGNGTLT 200
CVAELKDLQA SKSLTVNLTV VQPPPDSIGE EGPALPTWAI ILLAVAFSLL 250
LILIIVLIII FCCCCASRRE KEESTYQNEI RKSANMRTNK ADPETKLKGG 300
KENYGYSSDE AKAAQTASLP PKSAEVSLPE KRSSSLPYQE LNKHQPGPAT 350
HPRVSFDIAS PQKVRNVTLV 370
Length:370
Mass (Da):40,292
Last modified:October 1, 2003 - v1
Checksum:i001B40AC535C96EF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 225100Missing in BAB25436. 1 Publication
Add
BLAST
Sequence conflicti126 – 225100Missing in AAH04806. 1 Publication
Add
BLAST
Sequence conflicti299 – 2991G → S in BAB25436. 1 Publication
Sequence conflicti299 – 2991G → S in AAH04806. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF537215 mRNA. Translation: AAP49218.1.
AK008060 mRNA. Translation: BAB25436.1.
BC004806 mRNA. Translation: AAH04806.1.
CCDSiCCDS49924.1.
RefSeqiNP_082354.1. NM_028078.3.
UniGeneiMm.119714.

Genome annotation databases

EnsembliENSMUST00000081093; ENSMUSP00000079874; ENSMUSG00000000159.
GeneIDi72058.
KEGGimmu:72058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF537215 mRNA. Translation: AAP49218.1 .
AK008060 mRNA. Translation: BAB25436.1 .
BC004806 mRNA. Translation: AAH04806.1 .
CCDSi CCDS49924.1.
RefSeqi NP_082354.1. NM_028078.3.
UniGenei Mm.119714.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VQG X-ray 1.35 B 363-370 [» ]
ProteinModelPortali Q7TSN7.
ModBasei Search...

Protein-protein interaction databases

BioGridi 215124. 3 interactions.

PTM databases

PhosphoSitei Q7TSN7.

Proteomic databases

PaxDbi Q7TSN7.
PRIDEi Q7TSN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081093 ; ENSMUSP00000079874 ; ENSMUSG00000000159 .
GeneIDi 72058.
KEGGi mmu:72058.

Organism-specific databases

CTDi 150084.
MGIi MGI:1919308. Igsf5.

Phylogenomic databases

eggNOGi NOG47258.
GeneTreei ENSGT00390000018711.
HOGENOMi HOG000113018.
HOVERGENi HBG059033.
KOi K06786.

Miscellaneous databases

ChiTaRSi IGSF5. mouse.
NextBioi 335340.
PROi Q7TSN7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q7TSN7.
Bgeei Q7TSN7.
CleanExi MM_IGSF5.
Genevestigatori Q7TSN7.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view ]
Pfami PF07679. I-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 2 hits.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "JAM4, a junctional cell adhesion molecule interacting with a tight junction protein, MAGI-1."
    Hirabayashi S., Tajima M., Yao I., Nishimura W., Mori H., Hata Y.
    Mol. Cell. Biol. 23:4267-4282(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MAGI1, TISSUE SPECIFICITY, GLYCOSYLATION.
    Strain: Swiss Webster.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "A CTX family cell adhesion molecule, JAM4, is expressed in stem cell and progenitor cell populations of both male germ cell and hematopoietic cell lineages."
    Nagamatsu G., Ohmura M., Mizukami T., Hamaguchi I., Hirabayashi S., Yoshida S., Hata Y., Suda T., Ohbo K.
    Mol. Cell. Biol. 26:8498-8506(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. "Ligand-of-Numb protein X is an endocytic scaffold for junctional adhesion molecule 4."
    Kansaku A., Hirabayashi S., Mori H., Fujiwara N., Kawata A., Ikeda M., Rokukawa C., Kurihara H., Hata Y.
    Oncogene 25:5071-5084(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LNX1 ISOFORM 2.

Entry informationi

Entry nameiIGSF5_MOUSE
AccessioniPrimary (citable) accession number: Q7TSN7
Secondary accession number(s): Q9D8G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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