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Q7TSJ2

- MAP6_MOUSE

UniProt

Q7TSJ2 - MAP6_MOUSE

Protein

Microtubule-associated protein 6

Gene

Map6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Involved in microtubule stabilization in many cell types, including neuronal cells. Specifically has microtubule cold stabilizing activity. Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with TMEM106B.1 Publication

    GO - Molecular functioni

    1. microtubule binding Source: MGI

    GO - Biological processi

    1. dendrite morphogenesis Source: UniProtKB
    2. lysosome localization Source: UniProtKB
    3. microtubule cytoskeleton organization Source: InterPro
    4. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated protein 6
    Short name:
    MAP-6
    Alternative name(s):
    Stable tubule-only polypeptide
    Short name:
    STOP
    Gene namesi
    Name:Map6
    Synonyms:Mtap6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1201690. Map6.

    Subcellular locationi

    Cytoplasmcytoskeleton. Golgi apparatus
    Note: Isoform 1 and isoform 2 associate with axonal microtubules in neurons. Isoform 3 associates with microtubules in fibroblasts.

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell
    2. microtubule Source: UniProtKB-KW
    3. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

    Pathology & Biotechi

    Disruption phenotypei

    Mice are devoid of cold-stable microtubules and show no detectable defects in brain anatomy but show synaptic defects, with depleted synaptic vesicle pools and impaired synaptic plasticity, associated with severe behavioral disorders, including a disorganized activity with disruption of normal behavioral sequences and episodes of hyperlocomotion or apparent prostration, anxiety, severe social withdrawal and complete nurturing defects. The behavioral defects are alleviated by long-term treatment with neuroleptics.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 906906Microtubule-associated protein 6PRO_0000344045Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi5 – 51S-palmitoyl cysteineBy similarity
    Lipidationi10 – 101S-palmitoyl cysteineBy similarity
    Lipidationi11 – 111S-palmitoyl cysteineBy similarity
    Modified residuei141 – 1411Phosphotyrosine1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiQ7TSJ2.
    PaxDbiQ7TSJ2.
    PRIDEiQ7TSJ2.

    Expressioni

    Tissue specificityi

    Isoform 1 is specifically expressed in adult brain. Isoform 2 is predominantly expressed in embryonic brain; expression persists at low levels in the adult brain. Isoform 3 is expressed at high levels in lung and at lower levels in testis, heart, muscle and kidney (at protein level). Oligodendrocytes express a major isoform of 89 kDa (O-STOP). Astrocytes also express an isoform of 60 kDa (A-STOP).2 Publications

    Developmental stagei

    Isoform 2 is expressed in embryonic brain.1 Publication

    Gene expression databases

    ArrayExpressiQ7TSJ2.
    BgeeiQ7TSJ2.
    GenevestigatoriQ7TSJ2.

    Interactioni

    Subunit structurei

    Interacts with calmodulin (via C-terminus); the interaction is dependent on Ca2+. Interacts with TMEM106B By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201587. 4 interactions.
    IntActiQ7TSJ2. 5 interactions.
    MINTiMINT-4105179.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TSJ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati222 – 26746Mc-1Add
    BLAST
    Repeati268 – 31346Mc-2Add
    BLAST
    Repeati314 – 35946Mc-3Add
    BLAST
    Repeati360 – 40546Mc-4Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1515Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni116 – 13924Mn 1By similarityAdd
    BLAST
    Regioni124 – 13815Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni151 – 17424Mn 2By similarityAdd
    BLAST
    Regioni160 – 17415Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni187 – 20115Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni222 – 4051844 X approximate tandem repeat McAdd
    BLAST
    Regioni235 – 24915Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni280 – 29415Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni325 – 33915Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni375 – 38915Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni427 – 45024Mn 3By similarityAdd
    BLAST
    Regioni435 – 44915Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni486 – 50015Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni513 – 52715Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the STOP family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG87903.
    GeneTreeiENSGT00530000063947.
    HOGENOMiHOG000113479.
    HOVERGENiHBG053112.
    InParanoidiQ7TSJ2.
    KOiK10432.
    OMAiRKAGPAW.
    OrthoDBiEOG7ZWD1D.
    PhylomeDBiQ7TSJ2.
    TreeFamiTF338320.

    Family and domain databases

    InterProiIPR007882. MAP6.
    IPR024963. MAP6/FAM154.
    [Graphical view]
    PANTHERiPTHR14759. PTHR14759. 1 hit.
    PfamiPF05217. STOP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Note: Comment: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q7TSJ2-1) [UniParc]FASTAAdd to Basket

    Also known as: N-STOP, Neuronal STOP

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAWPCITRAC CIARFWNQLD KADIAVPLVF TKYSEATEHP GAPPQPPAPL    50
    QPALAPPSRA VAIETQPAQG ESDAVARATG PAPGPSVDRE TVAAPGRSGL 100
    GLGAASASTS GSGPADSVMR QDYRAWKVQR PEPSCRPRSE YQPSDAPFER 150
    ETQYQKDFRA WPLPRRGDHP WIPKPVQIPA TSQPSQPVLG VPKRRPQSQE 200
    RGPMQLSADA RDPEGAGGAG VLAAGKASGV DQRDTRRKAG PAWMVTRNEG 250
    HEEKPLPPAQ SQTQEGGPAA GKASGADQRD TRRKAGPAWM VTRSEGHEEK 300
    PLPPAQSQTQ EGGPAAGKAS GADQRDTRRK AGPAWMVTRT EGHEETPLPP 350
    AQSQTQEGGP AAGKASGADE RDTRRKAGPA WMVRRSEGHE QTPAAHAQGT 400
    GPEGGKGRAV ADALNRQIRE EVASTVSSSY RNEFRAWTDI KPVKPIKAKP 450
    QYKPPDDKMV HETSYSAQFK GEANKPSAAD NKAMDRRRIR SLYSEPFKEC 500
    PKVEKPSVQS SKPKKTSTSH KPPRKAKDKQ VVSGQAAKKK TTEGPSATKP 550
    DDKEQSKEMN NKLAEAKESR VKPTSDASKN RGPVTKEPHK DQGSVAPGLP 600
    KGQEPLKDQG PVVPGLPKDQ VPVVPGSLKG QSPTAPGPTK DQGAVLLGPV 650
    KDLGPVAPAP IKVQDHIASE LLKNKDSVPL APAKAQSPLL PEPLKNQSPV 700
    VPASTKDQSF PTPAPRKDPG PVIPEPEKDR APTVPERRKD QHVSIMASLK 750
    NEAPMVPESV KNQGLAGPEL VKDTGTDTTA PRYLKGHDSV FVAPVKNQGP 800
    VIPEPVKSQD PIIPALAKDQ GPMLPEPPKN QSPVVLGPIK NQDPIIPVPL 850
    KGQDPLVPAP TKDQGPTAPD PLKTQGPKGT QLPTVSPSPP VMIPTVPHTE 900
    YIEGSP 906

    Note: Produced by alternative promoter usage.

    Length:906
    Mass (Da):96,450
    Last modified:July 22, 2008 - v2
    Checksum:iF3CED1FCF1E2CD83
    GO
    Isoform 2 (identifier: Q7TSJ2-2) [UniParc]FASTAAdd to Basket

    Also known as: E-STOP, Early STOP

    The sequence of this isoform differs from the canonical sequence as follows:
         569-906: Missing.

    Note: Produced by alternative splicing of isoform 1.

    Show »
    Length:568
    Mass (Da):61,105
    Checksum:i81B1DFF1B51D2658
    GO
    Isoform 3 (identifier: Q7TSJ2-3) [UniParc]FASTAAdd to Basket

    Also known as: F-STOP, Fibroblastic STOP

    The sequence of this isoform differs from the canonical sequence as follows:
         1-203: Missing.
         503-509: VEKPSVQ → EPGQTHQ
         510-906: Missing.

    Note: Produced by alternative promoter usage.

    Show »
    Length:306
    Mass (Da):32,803
    Checksum:iE19396DFF272B496
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti81 – 811P → L in CAA75049. (PubMed:9501006)Curated
    Sequence conflicti87 – 871V → G in CAA75930. (PubMed:9501006)Curated
    Sequence conflicti344 – 3441E → K in CAA75930. (PubMed:9501006)Curated
    Sequence conflicti344 – 3441E → K in CAA75989. (PubMed:9600916)Curated
    Sequence conflicti405 – 4051G → S in CAA75049. (PubMed:9501006)Curated
    Sequence conflicti755 – 7573MVP → VVH in CAA75930. (PubMed:9501006)Curated
    Sequence conflicti755 – 7551M → V in CAA75049. (PubMed:9501006)Curated
    Sequence conflicti825 – 8251P → S in CAA75930. (PubMed:9501006)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 203203Missing in isoform 3. 1 PublicationVSP_034724Add
    BLAST
    Alternative sequencei503 – 5097VEKPSVQ → EPGQTHQ in isoform 3. 1 PublicationVSP_034725
    Alternative sequencei510 – 906397Missing in isoform 3. 1 PublicationVSP_034726Add
    BLAST
    Alternative sequencei569 – 906338Missing in isoform 2. 1 PublicationVSP_034727Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14754, Y14755, Y14756 Genomic DNA. Translation: CAA75049.1.
    Y16008 Genomic DNA. Translation: CAA75930.1.
    Y16032 mRNA. Translation: CAA75989.1.
    BC053039 mRNA. Translation: AAH53039.1.
    CCDSiCCDS21479.1. [Q7TSJ2-1]
    CCDS40029.1. [Q7TSJ2-3]
    PIRiJC5963.
    RefSeqiNP_001036820.2. NM_001043355.2. [Q7TSJ2-3]
    NP_001041632.1. NM_001048167.1. [Q7TSJ2-2]
    NP_034967.2. NM_010837.3. [Q7TSJ2-1]
    UniGeneiMm.154087.

    Genome annotation databases

    EnsembliENSMUST00000068973; ENSMUSP00000064787; ENSMUSG00000055407. [Q7TSJ2-1]
    ENSMUST00000107100; ENSMUSP00000102717; ENSMUSG00000055407. [Q7TSJ2-3]
    GeneIDi17760.
    KEGGimmu:17760.
    UCSCiuc009ilg.1. mouse. [Q7TSJ2-1]
    uc009ili.1. mouse. [Q7TSJ2-3]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14754 , Y14755 , Y14756 Genomic DNA. Translation: CAA75049.1 .
    Y16008 Genomic DNA. Translation: CAA75930.1 .
    Y16032 mRNA. Translation: CAA75989.1 .
    BC053039 mRNA. Translation: AAH53039.1 .
    CCDSi CCDS21479.1. [Q7TSJ2-1 ]
    CCDS40029.1. [Q7TSJ2-3 ]
    PIRi JC5963.
    RefSeqi NP_001036820.2. NM_001043355.2. [Q7TSJ2-3 ]
    NP_001041632.1. NM_001048167.1. [Q7TSJ2-2 ]
    NP_034967.2. NM_010837.3. [Q7TSJ2-1 ]
    UniGenei Mm.154087.

    3D structure databases

    ProteinModelPortali Q7TSJ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201587. 4 interactions.
    IntActi Q7TSJ2. 5 interactions.
    MINTi MINT-4105179.

    Proteomic databases

    MaxQBi Q7TSJ2.
    PaxDbi Q7TSJ2.
    PRIDEi Q7TSJ2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068973 ; ENSMUSP00000064787 ; ENSMUSG00000055407 . [Q7TSJ2-1 ]
    ENSMUST00000107100 ; ENSMUSP00000102717 ; ENSMUSG00000055407 . [Q7TSJ2-3 ]
    GeneIDi 17760.
    KEGGi mmu:17760.
    UCSCi uc009ilg.1. mouse. [Q7TSJ2-1 ]
    uc009ili.1. mouse. [Q7TSJ2-3 ]

    Organism-specific databases

    CTDi 4135.
    MGIi MGI:1201690. Map6.

    Phylogenomic databases

    eggNOGi NOG87903.
    GeneTreei ENSGT00530000063947.
    HOGENOMi HOG000113479.
    HOVERGENi HBG053112.
    InParanoidi Q7TSJ2.
    KOi K10432.
    OMAi RKAGPAW.
    OrthoDBi EOG7ZWD1D.
    PhylomeDBi Q7TSJ2.
    TreeFami TF338320.

    Miscellaneous databases

    NextBioi 292441.
    PROi Q7TSJ2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q7TSJ2.
    Bgeei Q7TSJ2.
    Genevestigatori Q7TSJ2.

    Family and domain databases

    InterProi IPR007882. MAP6.
    IPR024963. MAP6/FAM154.
    [Graphical view ]
    PANTHERi PTHR14759. PTHR14759. 1 hit.
    Pfami PF05217. STOP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic structure and chromosomal mapping of the mouse STOP gene (Mtap6)."
      Denarier E., Aguezzoul M., Jolly C., Vourc'h C., Roure A., Andrieux A., Bosc C., Job D.
      Biochem. Biophys. Res. Commun. 243:791-796(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
      Strain: 129/SvJ.
      Tissue: Liver.
    2. "Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts."
      Denarier E., Fourest-Lieuvin A., Bosc C., Pirollet F., Chapel A., Margolis R.L., Job D.
      Proc. Natl. Acad. Sci. U.S.A. 95:6055-6060(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION.
      Strain: Swiss Webster / NIH.
      Tissue: Fibroblast.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain.
    4. "The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders."
      Andrieux A., Salin P.A., Vernet M., Kujala P., Baratier J., Gory-Faure S., Bosc C., Pointu H., Proietto D., Schweitzer A., Denarier E., Klumperman J., Job D.
      Genes Dev. 16:2350-2364(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Unusual Ca(2+)-calmodulin binding interactions of the microtubule-associated protein F-STOP."
      Bouvier D., Vanhaverbeke C., Simorre J.P., Arlaud G.J., Bally I., Forge V., Margolis R.L., Gans P., Kleman J.P.
      Biochemistry 42:11484-11493(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN.
    6. "Overlap of promoter and coding sequences in the mouse STOP gene (Mtap6)."
      Aguezzoul M., Andrieux A., Denarier E.
      Genomics 81:623-627(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE PROMOTER USAGE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    7. "Astrocytes and oligodendrocytes express different STOP protein isoforms."
      Galiano M.R., Bosc C., Schweitzer A., Andrieux A., Job D., Hallak M.E.
      J. Neurosci. Res. 78:329-337(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    8. "STOP-like protein 21 is a novel member of the STOP family, revealing a Golgi localization of STOP proteins."
      Gory-Faure S., Windscheid V., Bosc C., Peris L., Proietto D., Franck R., Denarier E., Job D., Andrieux A.
      J. Biol. Chem. 281:28387-28396(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    10. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    11. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiMAP6_MOUSE
    AccessioniPrimary (citable) accession number: Q7TSJ2
    Secondary accession number(s): O55129
    , O70586, O70587, Q78DV4, Q78DV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3