Microtubule-associated protein 6 - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

Q7TSJ2 (MAP6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Microtubule-associated protein 6
Short name=MAP-6

Alternative name(s):
Stable tubule-only polypeptide
Short name=STOP

Gene names

Name:	Map6
Synonyms:	Mtap6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in microtubule stabilization in many cell types, including neuronal cells. Specifically has microtubule cold stabilizing activity. Ref.2
Subunit structure	Interacts with calmodulin (via C-terminus); the interaction is dependent on Ca²⁺. Ref.5
Subcellular location	Cytoplasm › cytoskeleton. Golgi apparatus. Note: Isoform 1 and isoform 2 associate with axonal microtubules in neurons. Isoform 3 associates with microtubules in fibroblasts. Ref.2 Ref.8
Tissue specificity	Isoform 1 is specifically expressed in adult brain. Isoform 2 is predominantly expressed in embryonic brain; expression persists at low levels in the adult brain. Isoform 3 is expressed at high levels in lung and at lower levels in testis, heart, muscle and kidney (at protein level). Oligodendrocytes express a major isoform of 89 kDa (O-STOP). Astrocytes also express an isoform of 60 kDa (A-STOP). Ref.6 Ref.7
Developmental stage	Isoform 2 is expressed in embryonic brain. Ref.6
Disruption phenotype	Mice are devoid of cold-stable microtubules and show no detectable defects in brain anatomy but show synaptic defects, with depleted synaptic vesicle pools and impaired synaptic plasticity, associated with severe behavioral disorders, including a disorganized activity with disruption of normal behavioral sequences and episodes of hyperlocomotion or apparent prostration, anxiety, severe social withdrawal and complete nurturing defects. The behavioral defects are alleviated by long-term treatment with neuroleptics. Ref.4
Sequence similarities	Belongs to the STOP family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Microtubule
Coding sequence diversity	Alternative promoter usage Alternative splicing
Domain	Repeat
Ligand	Calmodulin-binding
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of microtubule depolymerization Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	microtubule binding Inferred from direct assay Ref.8. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select] Note: Comment: Additional isoforms seem to exist.

Isoform 1 (identifier: Q7TSJ2-1) Also known as: N-STOP; Neuronal STOP; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.

Isoform 2 (identifier: Q7TSJ2-2) Also known as: E-STOP; Early STOP; The sequence of this isoform differs from the canonical sequence as follows: 569-906: Missing.
Note: Produced by alternative splicing of isoform 1.

Isoform 3 (identifier: Q7TSJ2-3) Also known as: F-STOP; Fibroblastic STOP; The sequence of this isoform differs from the canonical sequence as follows: 1-203: Missing. 503-509: VEKPSVQ → EPGQTHQ 510-906: Missing.
Note: Produced by alternative promoter usage.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 906

906

Microtubule-associated protein 6

PRO_0000344045

Regions

Repeat

222 – 267

Mc-1

Repeat

268 – 313

Mc-2

Repeat

314 – 359

Mc-3

Repeat

360 – 405

Mc-4

Region

1 – 15

Calmodulin-binding By similarity

Region

116 – 139

Mn 1 By similarity

Region

124 – 138

Calmodulin-binding By similarity

Region

151 – 174

Mn 2 By similarity

Region

160 – 174

Calmodulin-binding By similarity

Region

187 – 201

Calmodulin-binding By similarity

Region

222 – 405

184

4 X approximate tandem repeat Mc

Region

235 – 249

Calmodulin-binding By similarity

Region

280 – 294

Calmodulin-binding By similarity

Region

325 – 339

Calmodulin-binding By similarity

Region

375 – 389

Calmodulin-binding By similarity

Region

427 – 450

Mn 3 By similarity

Region

435 – 449

Calmodulin-binding By similarity

Region

486 – 500

Calmodulin-binding By similarity

Region

513 – 527

Calmodulin-binding By similarity

Amino acid modifications

Modified residue

108

Phosphoserine Ref.9

Modified residue

141

Phosphotyrosine Ref.10

Modified residue

632

Phosphoserine Ref.9

Modified residue

687

Phosphoserine Ref.9

Modified residue

832

Phosphoserine Ref.9

Modified residue

905

Phosphoserine Ref.9

Lipidation

S-palmitoyl cysteine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Natural variations

Alternative sequence

1 – 203

203

Missing in isoform 3.

VSP_034724

Alternative sequence

503 – 509

VEKPSVQ → EPGQTHQ in isoform 3.

VSP_034725

Alternative sequence

510 – 906

397

Missing in isoform 3.

VSP_034726

Alternative sequence

569 – 906

338

Missing in isoform 2.

VSP_034727

Experimental info

Sequence conflict

P → L in CAA75049. Ref.1

Sequence conflict

V → G in CAA75930. Ref.1

Sequence conflict

344

E → K in CAA75930. Ref.1

Sequence conflict

344

E → K in CAA75989. Ref.2

Sequence conflict

405

G → S in CAA75049. Ref.1

Sequence conflict

755 – 757

MVP → VVH in CAA75930. Ref.1

Sequence conflict

755

M → V in CAA75049. Ref.1

Sequence conflict

825

P → S in CAA75930. Ref.1

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (N-STOP) (Neuronal STOP) [UniParc]. Last modified July 22, 2008. Version 2. Checksum: F3CED1FCF1E2CD83 Show »	FASTA	906	96,450
10 20 30 40 50 60 MAWPCITRAC CIARFWNQLD KADIAVPLVF TKYSEATEHP GAPPQPPAPL QPALAPPSRA 70 80 90 100 110 120 VAIETQPAQG ESDAVARATG PAPGPSVDRE TVAAPGRSGL GLGAASASTS GSGPADSVMR 130 140 150 160 170 180 QDYRAWKVQR PEPSCRPRSE YQPSDAPFER ETQYQKDFRA WPLPRRGDHP WIPKPVQIPA 190 200 210 220 230 240 TSQPSQPVLG VPKRRPQSQE RGPMQLSADA RDPEGAGGAG VLAAGKASGV DQRDTRRKAG 250 260 270 280 290 300 PAWMVTRNEG HEEKPLPPAQ SQTQEGGPAA GKASGADQRD TRRKAGPAWM VTRSEGHEEK 310 320 330 340 350 360 PLPPAQSQTQ EGGPAAGKAS GADQRDTRRK AGPAWMVTRT EGHEETPLPP AQSQTQEGGP 370 380 390 400 410 420 AAGKASGADE RDTRRKAGPA WMVRRSEGHE QTPAAHAQGT GPEGGKGRAV ADALNRQIRE 430 440 450 460 470 480 EVASTVSSSY RNEFRAWTDI KPVKPIKAKP QYKPPDDKMV HETSYSAQFK GEANKPSAAD 490 500 510 520 530 540 NKAMDRRRIR SLYSEPFKEC PKVEKPSVQS SKPKKTSTSH KPPRKAKDKQ VVSGQAAKKK 550 560 570 580 590 600 TTEGPSATKP DDKEQSKEMN NKLAEAKESR VKPTSDASKN RGPVTKEPHK DQGSVAPGLP 610 620 630 640 650 660 KGQEPLKDQG PVVPGLPKDQ VPVVPGSLKG QSPTAPGPTK DQGAVLLGPV KDLGPVAPAP 670 680 690 700 710 720 IKVQDHIASE LLKNKDSVPL APAKAQSPLL PEPLKNQSPV VPASTKDQSF PTPAPRKDPG 730 740 750 760 770 780 PVIPEPEKDR APTVPERRKD QHVSIMASLK NEAPMVPESV KNQGLAGPEL VKDTGTDTTA 790 800 810 820 830 840 PRYLKGHDSV FVAPVKNQGP VIPEPVKSQD PIIPALAKDQ GPMLPEPPKN QSPVVLGPIK 850 860 870 880 890 900 NQDPIIPVPL KGQDPLVPAP TKDQGPTAPD PLKTQGPKGT QLPTVSPSPP VMIPTVPHTE YIEGSP « Hide
Isoform 2 (E-STOP) (Early STOP) [UniParc]. Checksum: 81B1DFF1B51D2658 Show »	FASTA	568	61,105
10 20 30 40 50 60 MAWPCITRAC CIARFWNQLD KADIAVPLVF TKYSEATEHP GAPPQPPAPL QPALAPPSRA 70 80 90 100 110 120 VAIETQPAQG ESDAVARATG PAPGPSVDRE TVAAPGRSGL GLGAASASTS GSGPADSVMR 130 140 150 160 170 180 QDYRAWKVQR PEPSCRPRSE YQPSDAPFER ETQYQKDFRA WPLPRRGDHP WIPKPVQIPA 190 200 210 220 230 240 TSQPSQPVLG VPKRRPQSQE RGPMQLSADA RDPEGAGGAG VLAAGKASGV DQRDTRRKAG 250 260 270 280 290 300 PAWMVTRNEG HEEKPLPPAQ SQTQEGGPAA GKASGADQRD TRRKAGPAWM VTRSEGHEEK 310 320 330 340 350 360 PLPPAQSQTQ EGGPAAGKAS GADQRDTRRK AGPAWMVTRT EGHEETPLPP AQSQTQEGGP 370 380 390 400 410 420 AAGKASGADE RDTRRKAGPA WMVRRSEGHE QTPAAHAQGT GPEGGKGRAV ADALNRQIRE 430 440 450 460 470 480 EVASTVSSSY RNEFRAWTDI KPVKPIKAKP QYKPPDDKMV HETSYSAQFK GEANKPSAAD 490 500 510 520 530 540 NKAMDRRRIR SLYSEPFKEC PKVEKPSVQS SKPKKTSTSH KPPRKAKDKQ VVSGQAAKKK 550 560 TTEGPSATKP DDKEQSKEMN NKLAEAKE « Hide
Isoform 3 (F-STOP) (Fibroblastic STOP) [UniParc]. Checksum: E19396DFF272B496 Show »	FASTA	306	32,803
10 20 30 40 50 60 MQLSADARDP EGAGGAGVLA AGKASGVDQR DTRRKAGPAW MVTRNEGHEE KPLPPAQSQT 70 80 90 100 110 120 QEGGPAAGKA SGADQRDTRR KAGPAWMVTR SEGHEEKPLP PAQSQTQEGG PAAGKASGAD 130 140 150 160 170 180 QRDTRRKAGP AWMVTRTEGH EETPLPPAQS QTQEGGPAAG KASGADERDT RRKAGPAWMV 190 200 210 220 230 240 RRSEGHEQTP AAHAQGTGPE GGKGRAVADA LNRQIREEVA STVSSSYRNE FRAWTDIKPV 250 260 270 280 290 300 KPIKAKPQYK PPDDKMVHET SYSAQFKGEA NKPSAADNKA MDRRRIRSLY SEPFKECPKE PGQTHQ « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure and chromosomal mapping of the mouse STOP gene (Mtap6)." Denarier E., Aguezzoul M., Jolly C., Vourc'h C., Roure A., Andrieux A., Bosc C., Job D. Biochem. Biophys. Res. Commun. 243:791-796(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2). Strain: 129/SvJ. Tissue: Liver.
[2]	"Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts." Denarier E., Fourest-Lieuvin A., Bosc C., Pirollet F., Chapel A., Margolis R.L., Job D. Proc. Natl. Acad. Sci. U.S.A. 95:6055-6060(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION. Strain: Swiss Webster / NIH. Tissue: Fibroblast.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders." Andrieux A., Salin P.A., Vernet M., Kujala P., Baratier J., Gory-Faure S., Bosc C., Pointu H., Proietto D., Schweitzer A., Denarier E., Klumperman J., Job D. Genes Dev. 16:2350-2364(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[5]	"Unusual Ca(2+)-calmodulin binding interactions of the microtubule-associated protein F-STOP." Bouvier D., Vanhaverbeke C., Simorre J.P., Arlaud G.J., Bally I., Forge V., Margolis R.L., Gans P., Kleman J.P. Biochemistry 42:11484-11493(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CALMODULIN.
[6]	"Overlap of promoter and coding sequences in the mouse STOP gene (Mtap6)." Aguezzoul M., Andrieux A., Denarier E. Genomics 81:623-627(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE PROMOTER USAGE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[7]	"Astrocytes and oligodendrocytes express different STOP protein isoforms." Galiano M.R., Bosc C., Schweitzer A., Andrieux A., Job D., Hallak M.E. J. Neurosci. Res. 78:329-337(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[8]	"STOP-like protein 21 is a novel member of the STOP family, revealing a Golgi localization of STOP proteins." Gory-Faure S., Windscheid V., Bosc C., Peris L., Proietto D., Franck R., Denarier E., Job D., Andrieux A. J. Biol. Chem. 281:28387-28396(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108; SER-632; SER-687; SER-832 AND SER-905, MASS SPECTROMETRY. Tissue: Brain cortex.
[10]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-141, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14754, Y14755, Y14756 Genomic DNA. Translation: CAA75049.1. Y16008 Genomic DNA. Translation: CAA75930.1. Y16032 mRNA. Translation: CAA75989.1. BC053039 mRNA. Translation: AAH53039.1.
IPI	IPI00115833. IPI00788315. IPI00788359.
PIR	JC5963.
RefSeq	NP_001036820.2. NM_001043355.2. NP_001041632.1. NM_001048167.1. NP_034967.2. NM_010837.3.
UniGene	Mm.154087.
3D structure databases
ProteinModelPortal	Q7TSJ2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TSJ2. 3 interactions.
Proteomic databases
PaxDb	Q7TSJ2.
PRIDE	Q7TSJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068973; ENSMUSP00000064787; ENSMUSG00000055407. ENSMUST00000107100; ENSMUSP00000102717; ENSMUSG00000055407.
GeneID	17760.
KEGG	mmu:17760.
UCSC	uc009ilg.1. mouse. uc009ilh.1. mouse. uc009ili.1. mouse.
Organism-specific databases
CTD	4135.
MGI	MGI:1201690. Map6.
Phylogenomic databases
eggNOG	NOG87903.
GeneTree	ENSGT00530000063947.
HOGENOM	HOG000113479.
HOVERGEN	HBG053112.
InParanoid	Q7TSJ2.
KO	K10432.
OMA	WIPKPVQ.
OrthoDB	EOG4CC41W.
Gene expression databases
ArrayExpress	Q7TSJ2.
Bgee	Q7TSJ2.
Genevestigator	Q7TSJ2.
Family and domain databases
InterPro	IPR024963. STOP/FAM154. IPR007882. STOP/MAP6. [Graphical view]
PANTHER	PTHR14759. PTHR14759. 1 hit.
Pfam	PF05217. STOP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292441.
SOURCE	Search...

Entry information

Entry name

MAP6_MOUSE

Accession

Primary (citable) accession number: Q7TSJ2
Secondary accession number(s): O55129

Q78DV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents