Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pleckstrin homology domain-containing family M member 1

Gene

Plekhm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Involved in vesicular transport in the osteoclast (By similarity). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (By similarity). May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity).By similarityCurated1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1004 – 1058Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST55

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family M member 1
Short name:
PH domain-containing family M member 1
Gene namesi
Name:Plekhm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2443207. Plekhm1.

Subcellular locationi

  • Cytoplasm Curated
  • Endosome membrane By similarity
  • Lysosome By similarity
  • Lysosome membrane By similarity

  • Note: Localizes to the external membrane of autolysosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003093361 – 1074Pleckstrin homology domain-containing family M member 1Add BLAST1074

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphoserineCombined sources1
Modified residuei433PhosphoserineBy similarity1
Modified residuei436PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7TSI1.
PaxDbiQ7TSI1.
PeptideAtlasiQ7TSI1.
PRIDEiQ7TSI1.

PTM databases

iPTMnetiQ7TSI1.
PhosphoSitePlusiQ7TSI1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034247.
ExpressionAtlasiQ7TSI1. baseline and differential.
GenevisibleiQ7TSI1. MM.

Interactioni

Subunit structurei

May interact with sialyl-lex positive protein. Interacts with RAB7A (GTP-bound form). Interacts with VPS41, VPS11 and VPS39; indicative for an association with the HOPS complex; the interaction with VPS41 seems to require RAB7A. Interacts with GABARAP, GABARAPL, GABARAPL2, MAP1LC3A, MAP1LC3B and MAP1LC3C (By similarity). Interacts with PAFAH1B1; detected in osteoblasts (PubMed:22073305).By similarity1 Publication

Protein-protein interaction databases

IntActiQ7TSI1. 1 interactor.
STRINGi10090.ENSMUSP00000047327.

Structurei

3D structure databases

ProteinModelPortaliQ7TSI1.
SMRiQ7TSI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 182RUNPROSITE-ProRule annotationAdd BLAST143
Domaini551 – 642PH 1PROSITE-ProRule annotationAdd BLAST92
Domaini701 – 795PH 2PROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni672 – 1074Interaction with RAB7ABy similarityAdd BLAST403

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi649 – 655LIRBy similarity7

Domaini

The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and LC3A/B/C.By similarity

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 RUN domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1004 – 1058Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST55

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQT. Eukaryota.
ENOG4111EZ2. LUCA.
GeneTreeiENSGT00550000074570.
HOGENOMiHOG000247013.
HOVERGENiHBG056799.
InParanoidiQ7TSI1.
OMAiPAFFKII.
OrthoDBiEOG091G013Y.
PhylomeDBiQ7TSI1.
TreeFamiTF317067.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
IPR025258. Zf-RING_9.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF13901. zf-RING_9. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM01175. DUF4206. 1 hit.
SM00233. PH. 2 hits.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
PS50826. RUN. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TSI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVENGLDP RAAIQVIKKK LVGSVKALQK QHVSLDTVVT SEDGDANTMC
60 70 80 90 100
SALEAVFIHG LHAKHIRAEA GGKRKKHTHQ KALPQPVFWP LLKAITHRHI
110 120 130 140 150
VSDLEHLVFI NTDVGRCRAW LRLALNDGLM ECYLKLLLQE PARLCEYYQP
160 170 180 190 200
TALLRDAEEA EFLLSFLQGL TSLSFELSYK SAILNEWTLT PLSLSGLCPL
210 220 230 240 250
SELDPLTTSG AELQRKESLD SISHSSGSED IEVQHSGHKI RRNRKLTASS
260 270 280 290 300
LSLDTASSSQ LSCSLNSDSC LLQENGPKSP DHSEEPMSYD SDLGMANTDD
310 320 330 340 350
PDRSLQEVLS EFSKAQVNSA PSSGPNQEPD TPMFQTPLSL HSLATSTHLH
360 370 380 390 400
FEGSEELFPA HKSSGTSSGG HKHQLLPQET PDEKQLGTAQ AGPAQSTSDQ
410 420 430 440 450
QPSSPVGGAA GQGSGPWKAL EYGRVGPKLV VSSPTSPKGK SWISEDDFCR
460 470 480 490 500
PPQEPALKSA AGLCTSPVQD TPESRAALHG PFSQGPRKSC SLGALDKACV
510 520 530 540 550
PSQACGNAQP APAPAPAPAP APAPAPGVTQ DHKNFCVVHR RQMGLSNPFR
560 570 580 590 600
GLMKLGTVAR RGAMGIWKEF FCELSPLEFR LYLSDEERTC VESCSLLRCE
610 620 630 640 650
AVGPAHSDGR FELVFSGKKL ALRASSQDEA EDWLDRVREA LQKVRPQQED
660 670 680 690 700
EWVNIQYPDQ AEDAPEAPPD SLPPYSTLLP EPAGAQGMQL DWTSAQVPEP
710 720 730 740 750
DAIKESLLYL YADRTWVPYI FSLSLESLKC FRVRNNEKML SDSHGVETIR
760 770 780 790 800
DILPDTSLGG PAFFKIITAK AVLKLQAKNT EEATHWRDLV RKVLASYLES
810 820 830 840 850
AEEAVTLGGS LDEKCQEVLK FATRENGFLL QYLVAIPTEK GLDSQGCFCA
860 870 880 890 900
GCSRQIGFSF VRPKLCAFSG LYYCDFCHQD DASVIPARII HNWDLTKRPV
910 920 930 940 950
CRQALKFLAQ IRAQPLINLQ LVNASLYEHV ERMHLIGRSR EQLKLLGDYL
960 970 980 990 1000
GLCRSGALKE LCKRLSHRNY LLESPHRFSV ADLQQIAEGV YEGFLKALIE
1010 1020 1030 1040 1050
FASQHVYHCD LCTQRGFICQ ICHHQDIIFP FEFDTTVRCA ECRTVFHQSC
1060 1070
QAVVRKGCPR CARRRKYQEQ NVVS
Length:1,074
Mass (Da):118,535
Last modified:October 1, 2003 - v1
Checksum:i56EDA05514E4B9F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti742 – 744DSH → TRP in AAH38943 (PubMed:15489334).Curated3
Sequence conflicti768T → S in BAE32773 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK154703 mRNA. Translation: BAE32773.1.
AL772325, AL929552 Genomic DNA. Translation: CAM16600.1.
AL929552, AL772325 Genomic DNA. Translation: CAM17476.1.
BC038943 mRNA. Translation: AAH38943.1.
BC053079 mRNA. Translation: AAH53079.1.
CCDSiCCDS25518.1.
RefSeqiNP_898855.1. NM_183034.1.
UniGeneiMm.18556.
Mm.383308.

Genome annotation databases

EnsembliENSMUST00000041272; ENSMUSP00000047327; ENSMUSG00000034247.
GeneIDi353047.
KEGGimmu:353047.
UCSCiuc007lug.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK154703 mRNA. Translation: BAE32773.1.
AL772325, AL929552 Genomic DNA. Translation: CAM16600.1.
AL929552, AL772325 Genomic DNA. Translation: CAM17476.1.
BC038943 mRNA. Translation: AAH38943.1.
BC053079 mRNA. Translation: AAH53079.1.
CCDSiCCDS25518.1.
RefSeqiNP_898855.1. NM_183034.1.
UniGeneiMm.18556.
Mm.383308.

3D structure databases

ProteinModelPortaliQ7TSI1.
SMRiQ7TSI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TSI1. 1 interactor.
STRINGi10090.ENSMUSP00000047327.

PTM databases

iPTMnetiQ7TSI1.
PhosphoSitePlusiQ7TSI1.

Proteomic databases

MaxQBiQ7TSI1.
PaxDbiQ7TSI1.
PeptideAtlasiQ7TSI1.
PRIDEiQ7TSI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041272; ENSMUSP00000047327; ENSMUSG00000034247.
GeneIDi353047.
KEGGimmu:353047.
UCSCiuc007lug.1. mouse.

Organism-specific databases

CTDi9842.
MGIiMGI:2443207. Plekhm1.

Phylogenomic databases

eggNOGiENOG410KDQT. Eukaryota.
ENOG4111EZ2. LUCA.
GeneTreeiENSGT00550000074570.
HOGENOMiHOG000247013.
HOVERGENiHBG056799.
InParanoidiQ7TSI1.
OMAiPAFFKII.
OrthoDBiEOG091G013Y.
PhylomeDBiQ7TSI1.
TreeFamiTF317067.

Miscellaneous databases

ChiTaRSiPlekhm1. mouse.
PROiQ7TSI1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034247.
ExpressionAtlasiQ7TSI1. baseline and differential.
GenevisibleiQ7TSI1. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR004012. Run_dom.
IPR025258. Zf-RING_9.
[Graphical view]
PfamiPF02759. RUN. 1 hit.
PF13901. zf-RING_9. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM01175. DUF4206. 1 hit.
SM00233. PH. 2 hits.
SM00593. RUN. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
PS50826. RUN. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHM1_MOUSE
AccessioniPrimary (citable) accession number: Q7TSI1
Secondary accession number(s): Q3U3L2, Q8CHU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Sialyl-lex is a carcinoma associated antigen.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.