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Protein

Centriolar coiled-coil protein of 110 kDa

Gene

Ccp110

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:23141541). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (PubMed:26965371). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (By similarity).By similarity2 Publications

GO - Biological processi

  • cell projection organization Source: UniProtKB-KW
  • centriole replication Source: MGI
  • centrosome duplication Source: UniProtKB
  • ciliary basal body organization Source: UniProtKB
  • negative regulation of cilium assembly Source: UniProtKB
  • positive regulation of cilium assembly Source: UniProtKB
  • regulation of cytokinesis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Centriolar coiled-coil protein of 110 kDa
Alternative name(s):
Centrosomal protein of 110 kDa
Short name:
Cep110
Short name:
Cp110
Gene namesi
Name:Ccp110
Synonyms:Cep110, Cp110, Kiaa0419
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141942. Ccp110.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB
  • centrosome Source: MGI
  • cilium Source: UniProtKB-KW
  • microtubule organizing center Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Lethal within a few hours after birth with multiple defects in organogenesis. Mice show an early block in cilia formation and a phenotype reminiscent of human short rib-polydactyly syndrome.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10041004Centriolar coiled-coil protein of 110 kDaPRO_0000089461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei364 – 3641PhosphoserineCombined sources
Modified residuei370 – 3701PhosphoserineBy similarity
Modified residuei398 – 3981PhosphoserineCombined sources
Modified residuei550 – 5501PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CDKs.By similarity
Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7TSH4.
MaxQBiQ7TSH4.
PaxDbiQ7TSH4.
PRIDEiQ7TSH4.

PTM databases

iPTMnetiQ7TSH4.
PhosphoSiteiQ7TSH4.

Expressioni

Gene expression databases

BgeeiQ7TSH4.
CleanExiMM_6330503K22RIK.
MM_CEP110.
GenevisibleiQ7TSH4. MM.

Interactioni

Subunit structurei

Interacts with CALM1, CETN2, CEP76, CEP97, CEP104, CEP290, TALPID3. Seems to associate with discrete CETN2, CEP97 and CEP290-containing complexes. Interacts with NEURL4 and CCNF; these interactions are not mutually exclusive and both lead to CCP110 ubiquitination and proteasome-dependent degradation. Via its interaction with NEURL4, may indirectly interact with HERC2. Interacts with KIF24, leading to its recruitment to centrioles. Interacts with USP20 and USP33 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Calm3P622044EBI-646843,EBI-397460

Protein-protein interaction databases

BioGridi221690. 8 interactions.
IntActiQ7TSH4. 13 interactions.
STRINGi10090.ENSMUSP00000038881.

Structurei

3D structure databases

ProteinModelPortaliQ7TSH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 221221CEP97 bindingBy similarityAdd
BLAST
Regioni64 – 8219Calmodulin-bindingBy similarityAdd
BLAST
Regioni67 – 8216Required for interaction with CEP290By similarityAdd
BLAST
Regioni349 – 564216Interaction with CEP76By similarityAdd
BLAST
Regioni773 – 81341Calmodulin-bindingBy similarityAdd
BLAST
Regioni901 – 91616Calmodulin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili51 – 9040Sequence analysisAdd
BLAST
Coiled coili641 – 69959Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IF00. Eukaryota.
ENOG410XP98. LUCA.
GeneTreeiENSGT00390000004090.
HOVERGENiHBG050873.
InParanoidiQ7TSH4.
KOiK16453.
OMAiFYLWGSS.
OrthoDBiEOG7SV0XP.
PhylomeDBiQ7TSH4.
TreeFamiTF332788.

Family and domain databases

InterProiIPR033207. CCP110.
[Graphical view]
PANTHERiPTHR13594. PTHR13594. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7TSH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEYEEFCEK ALGRAQEASL STGSFLPAQA ESVSLIRFHG VAVLSPLLTI
60 70 80 90 100
EKRKKIQEEK QKALDVQSRK QANRKKALLT RVQEILENVQ VRKAPNASDF
110 120 130 140 150
DQWATETIYS NPEVTDLNVP VRVPNSLPSP TEHCTSVKLE KITGLLPVNN
160 170 180 190 200
EDQQTPKRVG LPGDSEVSGS LRQCESPESR QAEDGAALRL SSASPQETII
210 220 230 240 250
SDVLGKEEQD PSCLAEVTPD PYIMSLQNLM KRSKEYVERE LSSRSLRNSL
260 270 280 290 300
KRSVNETHSD RENDAAKASD CVKEKAPPMP IGRHCGSAIP DKPSLNKSNV
310 320 330 340 350
LLQGASQASS MGTAGLASFS KIDLPAGAAP PAAPDAGSDF TVIPTFVTEN
360 370 380 390 400
KVKSLKGPYA KLPSPEPSMS PTMHRRHSRS ASACQILINN PVNACELSPK
410 420 430 440 450
GKEEAVDRTA PAAAETTNES ETVPKSPTDL TGVCSSNVSA TKITSESTRE
460 470 480 490 500
MVVGKPSQRQ QALGAHLGNN VTVERSAMEG PFIADDRGAQ KVDGTCMAVP
510 520 530 540 550
KLHELQPSSQ CVSSQTLEDV CELKSASLLA KNSCNLQMEL NKSYDVKHPS
560 570 580 590 600
PLLTQTQTSR QQMDTPPVFR GNEQFVDNSF EKVKRRLDLD VDSLQKENCP
610 620 630 640 650
YIITAGVAEQ ERDRLLERRY PKGFVHINKN KMLETSPKEG QELLKSKMLA
660 670 680 690 700
FEEMRKRLEE QHAQQLSLLI AEQEREQEQL QKEIEEQEKM LKEKAVTTDV
710 720 730 740 750
SDLNSALEWR QRTDSALLET MLSQVDSLQT SNNSGFITSA LQYSFGSAGE
760 770 780 790 800
APFYLWGSLT SGVTRVSGTR PCGRAQAKWS QVFNPEIHAK FNKITAVAKG
810 820 830 840 850
FLTRKLMQTD KLKQLRQTVK DTMEFIRSFQ SEAPLKRGVV SAQDASLQER
860 870 880 890 900
VLAQLRAALY GIHDIFFVMD AAERMSILHH DREARKEKLL RQMDKMKSPR
910 920 930 940 950
VALSVATQKS LDRKKFMKVA EMGMPNKKFL LKQNPSETRV LQPNQGQNAP
960 970 980 990 1000
VHRLLSRQGT PKTSVKGVVQ NRQKPSQSRV PNRAPVSGAY AGKTQRKRPN

VATI
Length:1,004
Mass (Da):111,139
Last modified:October 1, 2003 - v1
Checksum:iC8D81F5F2FE213A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053103 mRNA. Translation: AAH53103.1.
AK172946 mRNA. Translation: BAD32224.1.
CCDSiCCDS21775.1.
RefSeqiNP_892040.1. NM_182995.1.
UniGeneiMm.23279.

Genome annotation databases

EnsembliENSMUST00000038650; ENSMUSP00000038881; ENSMUSG00000033904.
ENSMUST00000106557; ENSMUSP00000102167; ENSMUSG00000033904.
GeneIDi101565.
KEGGimmu:101565.
UCSCiuc009jkj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053103 mRNA. Translation: AAH53103.1.
AK172946 mRNA. Translation: BAD32224.1.
CCDSiCCDS21775.1.
RefSeqiNP_892040.1. NM_182995.1.
UniGeneiMm.23279.

3D structure databases

ProteinModelPortaliQ7TSH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221690. 8 interactions.
IntActiQ7TSH4. 13 interactions.
STRINGi10090.ENSMUSP00000038881.

PTM databases

iPTMnetiQ7TSH4.
PhosphoSiteiQ7TSH4.

Proteomic databases

EPDiQ7TSH4.
MaxQBiQ7TSH4.
PaxDbiQ7TSH4.
PRIDEiQ7TSH4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038650; ENSMUSP00000038881; ENSMUSG00000033904.
ENSMUST00000106557; ENSMUSP00000102167; ENSMUSG00000033904.
GeneIDi101565.
KEGGimmu:101565.
UCSCiuc009jkj.1. mouse.

Organism-specific databases

CTDi9738.
MGIiMGI:2141942. Ccp110.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IF00. Eukaryota.
ENOG410XP98. LUCA.
GeneTreeiENSGT00390000004090.
HOVERGENiHBG050873.
InParanoidiQ7TSH4.
KOiK16453.
OMAiFYLWGSS.
OrthoDBiEOG7SV0XP.
PhylomeDBiQ7TSH4.
TreeFamiTF332788.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ7TSH4.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TSH4.
CleanExiMM_6330503K22RIK.
MM_CEP110.
GenevisibleiQ7TSH4. MM.

Family and domain databases

InterProiIPR033207. CCP110.
[Graphical view]
PANTHERiPTHR13594. PTHR13594. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-959.
    Tissue: Embryonic intestine.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364 AND SER-398, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.
  4. "The spinocerebellar ataxia-associated gene tau tubulin kinase 2 controls the initiation of ciliogenesis."
    Goetz S.C., Liem K.F. Jr., Anderson K.V.
    Cell 151:847-858(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION.
  6. "Mother centriole distal appendages mediate centrosome docking at the immunological synapse and reveal mechanistic parallels with ciliogenesis."
    Stinchcombe J.C., Randzavola L.O., Angus K.L., Mantell J.M., Verkade P., Griffiths G.M.
    Curr. Biol. 25:3239-3244(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis."
    Yadav S.P., Sharma N.K., Liu C., Dong L., Li T., Swaroop A.
    Development 143:1491-1501(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiCP110_MOUSE
AccessioniPrimary (citable) accession number: Q7TSH4
Secondary accession number(s): Q6A072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: October 1, 2003
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.