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Protein

Zinc finger protein 516

Gene

Znf516

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator that binds to the promoter and activates the transcription of genes promoting brown adipose tissue (BAT) differentiation. Among brown adipose tissue-specific genes, binds the proximal region of the promoter of the UCP1 gene to activate its transcription and thereby regulate thermogenesis. May also play a role in the cellular response to replication stress (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 84C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri162 – 185C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri188 – 211C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri236 – 258C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri264 – 286C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri323 – 345C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri504 – 526C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri753 – 776C2H2-type 9; atypicalPROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1092 – 1114C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • DNA binding transcription factor activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • proximal promoter sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • adipose tissue development Source: UniProtKB
  • brown fat cell differentiation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • response to cold Source: UniProtKB
  • signal transduction Source: GO_Central
  • transcription by RNA polymerase II Source: GO_Central

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 516Imported
Gene namesi
Name:Znf516By similarity
Synonyms:Kiaa0222Imported, Zfp516Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2443957 Zfp516

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice die immediately after birth. E20.5 embryos display an impaired development of brown adipose tissue.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000476351 – 1157Zinc finger protein 516Add BLAST1157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki630Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki669Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1032Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1051Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ7TSH3
PaxDbiQ7TSH3
PeptideAtlasiQ7TSH3
PRIDEiQ7TSH3

PTM databases

iPTMnetiQ7TSH3
PhosphoSitePlusiQ7TSH3

Expressioni

Tissue specificityi

Expressed by adipocytes more specifically in brown adipose tissue compared to white adipose tissue (WAT).1 Publication

Inductioni

Up-regulated in response to cold in brown and subcutaneous white adipose tissue where it may regulate non-shivering thermogenesis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000058881
CleanExiMM_ZFP516
GenevisibleiQ7TSH3 MM

Interactioni

Subunit structurei

Interacts with PRDM16; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific genes.1 Publication

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi236692, 2 interactors
IntActiQ7TSH3, 2 interactors
STRINGi10090.ENSMUSP00000071216

Structurei

3D structure databases

ProteinModelPortaliQ7TSH3
SMRiQ7TSH3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 420Mediates promoter DNA-binding and activation of transcription1 PublicationAdd BLAST420

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 84C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri162 – 185C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri188 – 211C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri236 – 258C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri264 – 286C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri323 – 345C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri504 – 526C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri753 – 776C2H2-type 9; atypicalPROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1092 – 1114C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00530000063100
HOGENOMiHOG000155800
HOVERGENiHBG079384
InParanoidiQ7TSH3
KOiK22411
OMAiRDHITAQ
OrthoDBiEOG091G00XJ
PhylomeDBiQ7TSH3
TreeFamiTF332241

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 5 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits

Sequencei

Sequence statusi: Complete.

Q7TSH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRSREAEME LRRGPSPPRA GRSHEVDGDK AACHSCCICG KSFPFQSSLS
60 70 80 90 100
QHMRKHTGEK PYKCPYCDHR ASQKGNLKIH IRSHRTGTLI QGHEPEAGEA
110 120 130 140 150
QLGEMRVSEG LDGCASPTKS TSACNRVLNG AVPMDGSKIL LRSSRKEVEG
160 170 180 190 200
AASAQEDTEA TVPCSFCKSR FERKKDLELH VHQAHKPFKC RLCSYVTLRE
210 220 230 240 250
ESLLSHIERD HITAQVPNGS EACVENGKPE LSPGEFPCEV CGQAFSQTWF
260 270 280 290 300
LKAHMKKHRG SFDHGCHICG RRFKEPWFLK NHMKAHGPKA GSKNRPKSEL
310 320 330 340 350
DPIATINNVV QEEVIVAGLS LYEVCTKCGN LFTNLDSLNA HNAIHRKVEA
360 370 380 390 400
SRIRAPAEEG DSEDPLDTKQ FFLQCLNLTP YVAGDVSPGG QAGRRVAELD
410 420 430 440 450
PVNSYQAWQL ATRGKVAEPA EYLKYGTWDE ALAGDVAFDK DKREYILVSQ
460 470 480 490 500
EKRKREQDAP ATQAPPRKRA SVPGDPMLSG HLDPRPTSRP NRRASATTGQ
510 520 530 540 550
GKSSECFECG KIFRTYHQMV LHSRVHRRAR RDRDPEGDRA ARARCGSLSE
560 570 580 590 600
GDSASQPSSP GSACAIADSP GLAEEVVDDS GEEAVPEPAS GGQPRHCCSS
610 620 630 640 650
GEVTPTALSN GDQNHKLGNN LPEKDISEPK VGSAMPSVSI LENSSRETTK
660 670 680 690 700
GPEQHRYSLD LKMPAFHPKQ EVPSTTDRVD FPASMEITSL QHTLDSQAGH
710 720 730 740 750
SKEKLSDLHK EHCGVGKRAS APDLVPLDLS MRSSRDEPSG KEACSLQAAL
760 770 780 790 800
VIHPCPYCTH KTYYPEVLWM HKRIWHRVSC SSVAPPWTQP SGHKSIRSNL
810 820 830 840 850
VFLTRSGRTG PPPALGGKEC QPLLLSRFAR TQVPGGAPGS KGSSSPLGVT
860 870 880 890 900
TKAASMPKNK ESHSGGPCAL WASGPDGYRQ TRAGHGQEPP SAAVQGPLAK
910 920 930 940 950
PKQEGSSRLA PSPGSGSLSR STTPTPSVIT RVGAQPSANS KPVEKLGGPA
960 970 980 990 1000
VGTGFTPPNK HSAPDSLKAK FSPQPQGQPP LKGEGGSPLP PREPSVKAAQ
1010 1020 1030 1040 1050
ELRTLATCAA GSRGEAALQA PPGAPPTLNS AKQEPAAEGQ EKRLDILSIF
1060 1070 1080 1090 1100
KTYIPKDFAT LYQGWGVSSP GPEHRGTSLT GTPRTQAHQG DFVCVECGKS
1110 1120 1130 1140 1150
FHQPSQLRAH LRAHTVVFEC DGPRDSEVHT ASTDAPKQGR DHTTPGTVPA

GPLRKGI
Length:1,157
Mass (Da):124,788
Last modified:October 1, 2003 - v1
Checksum:iE14AEDEBF70E5100
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053104 mRNA Translation: AAH53104.1
AK129092 mRNA Translation: BAC97902.1
CCDSiCCDS29380.1
RefSeqiNP_001170935.1, NM_001177464.1
NP_898854.1, NM_183033.2
XP_006526561.1, XM_006526498.1
XP_006526562.1, XM_006526499.3
XP_006526563.1, XM_006526500.2
XP_006526564.1, XM_006526501.3
XP_006526565.1, XM_006526502.3
XP_006526566.1, XM_006526503.3
XP_011245387.1, XM_011247085.2
UniGeneiMm.326526

Genome annotation databases

EnsembliENSMUST00000071233; ENSMUSP00000071216; ENSMUSG00000058881
ENSMUST00000171238; ENSMUSP00000126629; ENSMUSG00000058881
GeneIDi329003
KEGGimmu:329003
UCSCiuc008fuf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiZN516_MOUSE
AccessioniPrimary (citable) accession number: Q7TSH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health