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Protein

Zinc finger protein 516

Gene

Znf516

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that binds to the promoter and activates the transcription of genes promoting brown adipose tissue (BAT) differentiation. Among brown adipose tissue-specific genes, binds the proximal region of the promoter of the UCP1 gene to activate its transcription and thereby regulate thermogenesis. May also play a role in the cellular response to replication stress (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri62 – 8423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 18524C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri188 – 21124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri236 – 25823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28623C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri323 – 34523C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri504 – 52623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri753 – 77624C2H2-type 9; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1092 – 111423C2H2-type 10PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • core promoter proximal region sequence-specific DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adipose tissue development Source: UniProtKB
  • brown fat cell differentiation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • response to cold Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 516Imported
Gene namesi
Name:Znf516By similarity
Synonyms:Kiaa0222Imported, Zfp516Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2443957. Zfp516.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice die immediately after birth. E20.5 embryos display an impaired development of brown adipose tissue.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11571157Zinc finger protein 516PRO_0000047635Add
BLAST

Proteomic databases

EPDiQ7TSH3.
MaxQBiQ7TSH3.
PaxDbiQ7TSH3.
PRIDEiQ7TSH3.

PTM databases

iPTMnetiQ7TSH3.
PhosphoSiteiQ7TSH3.

Expressioni

Tissue specificityi

Expressed by adipocytes more specifically in brown adipose tissue compared to white adipose tissue (WAT).1 Publication

Inductioni

Up-regulated in response to cold in brown and subcutaneous white adipose tissue where it may regulate non-shivering thermogenesis (at protein level).1 Publication

Gene expression databases

BgeeiQ7TSH3.
CleanExiMM_ZFP516.
GenevisibleiQ7TSH3. MM.

Interactioni

Subunit structurei

Interacts with PRDM16; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific genes.1 Publication

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi236692. 1 interaction.
IntActiQ7TSH3. 1 interaction.
STRINGi10090.ENSMUSP00000071216.

Structurei

3D structure databases

ProteinModelPortaliQ7TSH3.
SMRiQ7TSH3. Positions 34-346, 501-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 420420Mediates promoter DNA-binding and activation of transcription1 PublicationAdd
BLAST

Sequence similaritiesi

Contains 10 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri62 – 8423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 18524C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri188 – 21124C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri236 – 25823C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28623C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri323 – 34523C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri504 – 52623C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri753 – 77624C2H2-type 9; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1092 – 111423C2H2-type 10PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063100.
HOGENOMiHOG000155800.
HOVERGENiHBG079384.
InParanoidiQ7TSH3.
OMAiKYGAWDE.
OrthoDBiEOG76471T.
PhylomeDBiQ7TSH3.
TreeFamiTF332241.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TSH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRSREAEME LRRGPSPPRA GRSHEVDGDK AACHSCCICG KSFPFQSSLS
60 70 80 90 100
QHMRKHTGEK PYKCPYCDHR ASQKGNLKIH IRSHRTGTLI QGHEPEAGEA
110 120 130 140 150
QLGEMRVSEG LDGCASPTKS TSACNRVLNG AVPMDGSKIL LRSSRKEVEG
160 170 180 190 200
AASAQEDTEA TVPCSFCKSR FERKKDLELH VHQAHKPFKC RLCSYVTLRE
210 220 230 240 250
ESLLSHIERD HITAQVPNGS EACVENGKPE LSPGEFPCEV CGQAFSQTWF
260 270 280 290 300
LKAHMKKHRG SFDHGCHICG RRFKEPWFLK NHMKAHGPKA GSKNRPKSEL
310 320 330 340 350
DPIATINNVV QEEVIVAGLS LYEVCTKCGN LFTNLDSLNA HNAIHRKVEA
360 370 380 390 400
SRIRAPAEEG DSEDPLDTKQ FFLQCLNLTP YVAGDVSPGG QAGRRVAELD
410 420 430 440 450
PVNSYQAWQL ATRGKVAEPA EYLKYGTWDE ALAGDVAFDK DKREYILVSQ
460 470 480 490 500
EKRKREQDAP ATQAPPRKRA SVPGDPMLSG HLDPRPTSRP NRRASATTGQ
510 520 530 540 550
GKSSECFECG KIFRTYHQMV LHSRVHRRAR RDRDPEGDRA ARARCGSLSE
560 570 580 590 600
GDSASQPSSP GSACAIADSP GLAEEVVDDS GEEAVPEPAS GGQPRHCCSS
610 620 630 640 650
GEVTPTALSN GDQNHKLGNN LPEKDISEPK VGSAMPSVSI LENSSRETTK
660 670 680 690 700
GPEQHRYSLD LKMPAFHPKQ EVPSTTDRVD FPASMEITSL QHTLDSQAGH
710 720 730 740 750
SKEKLSDLHK EHCGVGKRAS APDLVPLDLS MRSSRDEPSG KEACSLQAAL
760 770 780 790 800
VIHPCPYCTH KTYYPEVLWM HKRIWHRVSC SSVAPPWTQP SGHKSIRSNL
810 820 830 840 850
VFLTRSGRTG PPPALGGKEC QPLLLSRFAR TQVPGGAPGS KGSSSPLGVT
860 870 880 890 900
TKAASMPKNK ESHSGGPCAL WASGPDGYRQ TRAGHGQEPP SAAVQGPLAK
910 920 930 940 950
PKQEGSSRLA PSPGSGSLSR STTPTPSVIT RVGAQPSANS KPVEKLGGPA
960 970 980 990 1000
VGTGFTPPNK HSAPDSLKAK FSPQPQGQPP LKGEGGSPLP PREPSVKAAQ
1010 1020 1030 1040 1050
ELRTLATCAA GSRGEAALQA PPGAPPTLNS AKQEPAAEGQ EKRLDILSIF
1060 1070 1080 1090 1100
KTYIPKDFAT LYQGWGVSSP GPEHRGTSLT GTPRTQAHQG DFVCVECGKS
1110 1120 1130 1140 1150
FHQPSQLRAH LRAHTVVFEC DGPRDSEVHT ASTDAPKQGR DHTTPGTVPA

GPLRKGI
Length:1,157
Mass (Da):124,788
Last modified:October 1, 2003 - v1
Checksum:iE14AEDEBF70E5100
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053104 mRNA. Translation: AAH53104.1.
AK129092 mRNA. Translation: BAC97902.1.
CCDSiCCDS29380.1.
RefSeqiNP_001170935.1. NM_001177464.1.
NP_898854.1. NM_183033.2.
XP_006526561.1. XM_006526498.1.
XP_006526562.1. XM_006526499.2.
XP_006526563.1. XM_006526500.1.
XP_006526564.1. XM_006526501.2.
XP_006526565.1. XM_006526502.2.
XP_006526566.1. XM_006526503.2.
XP_011245387.1. XM_011247085.1.
UniGeneiMm.326526.

Genome annotation databases

EnsembliENSMUST00000071233; ENSMUSP00000071216; ENSMUSG00000058881.
ENSMUST00000171238; ENSMUSP00000126629; ENSMUSG00000058881.
GeneIDi329003.
KEGGimmu:329003.
UCSCiuc008fuf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053104 mRNA. Translation: AAH53104.1.
AK129092 mRNA. Translation: BAC97902.1.
CCDSiCCDS29380.1.
RefSeqiNP_001170935.1. NM_001177464.1.
NP_898854.1. NM_183033.2.
XP_006526561.1. XM_006526498.1.
XP_006526562.1. XM_006526499.2.
XP_006526563.1. XM_006526500.1.
XP_006526564.1. XM_006526501.2.
XP_006526565.1. XM_006526502.2.
XP_006526566.1. XM_006526503.2.
XP_011245387.1. XM_011247085.1.
UniGeneiMm.326526.

3D structure databases

ProteinModelPortaliQ7TSH3.
SMRiQ7TSH3. Positions 34-346, 501-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236692. 1 interaction.
IntActiQ7TSH3. 1 interaction.
STRINGi10090.ENSMUSP00000071216.

PTM databases

iPTMnetiQ7TSH3.
PhosphoSiteiQ7TSH3.

Proteomic databases

EPDiQ7TSH3.
MaxQBiQ7TSH3.
PaxDbiQ7TSH3.
PRIDEiQ7TSH3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071233; ENSMUSP00000071216; ENSMUSG00000058881.
ENSMUST00000171238; ENSMUSP00000126629; ENSMUSG00000058881.
GeneIDi329003.
KEGGimmu:329003.
UCSCiuc008fuf.2. mouse.

Organism-specific databases

CTDi329003.
MGIiMGI:2443957. Zfp516.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063100.
HOGENOMiHOG000155800.
HOVERGENiHBG079384.
InParanoidiQ7TSH3.
OMAiKYGAWDE.
OrthoDBiEOG76471T.
PhylomeDBiQ7TSH3.
TreeFamiTF332241.

Miscellaneous databases

PROiQ7TSH3.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TSH3.
CleanExiMM_ZFP516.
GenevisibleiQ7TSH3. MM.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 735-1157.
    Tissue: Fetal brain.
  3. "Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat."
    Dempersmier J., Sambeat A., Gulyaeva O., Paul S.M., Hudak C.S., Raposo H.F., Kwan H.Y., Kang C., Wong R.H., Sul H.S.
    Mol. Cell 57:235-246(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PRDM16, SUBCELLULAR LOCATION, REGION, INDUCTION BY COLD, TISSUE SPECIFICITY.

Entry informationi

Entry nameiZN516_MOUSE
AccessioniPrimary (citable) accession number: Q7TSH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.