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Q7TSH2 (KPBB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphorylase b kinase regulatory subunit beta

Short name=Phosphorylase kinase subunit beta
Gene names
Name:Phkb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1085 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation By similarity.

Enzyme regulation

By phosphorylation of various serine residues By similarity.

Pathway

Glycan biosynthesis; glycogen metabolism.

Subunit structure

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Post-translational modification

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated By similarity.

Sequence similarities

Belongs to the phosphorylase b kinase regulatory chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10851085Phosphorylase b kinase regulatory subunit beta
PRO_0000057737

Regions

Region760 – 78728Calmodulin-binding Potential
Region912 – 94332Calmodulin-binding Potential

Amino acid modifications

Modified residue101Phosphoserine By similarity
Modified residue191Phosphoserine By similarity
Modified residue6931Phosphoserine By similarity
Lipidation10821S-farnesyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7TSH2 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 8EC52645AD89FDF5

FASTA1,085123,889
        10         20         30         40         50         60 
MANSPDAAFS SPALLRSGSV YEPLKSINLP RPDNETLWDK LDHYYRIVKS TMLMYQSPTT 

        70         80         90        100        110        120 
GLFPTKTCGG EEKSKVHESL YCAAGAWALA LAYRRIDDDK GRTHELEHSA IKCMRGILYC 

       130        140        150        160        170        180 
YMRQADKVQQ FKQDPRPTTC LHSVFSVHTG DELLSYEEYG HLQINAVSLF LLYLVEMISS 

       190        200        210        220        230        240 
GLQIIYNTDE VSFIQNLVFC VERVYRVPDF GVWERGSKYN NGSTELHSSS VGLAKAALEA 

       250        260        270        280        290        300 
INGFNLFGNQ GCSWSVIFVD LDAHNRNRQT LCSLLPRESR SHNTDAALLP CISYPAFALD 

       310        320        330        340        350        360 
DEALFSQTLD KVIRKLKGKY GFKRFLRDGY RTPLEDPNRR YYKPAEIKLF DGIECEFPIF 

       370        380        390        400        410        420 
FLYMMIDGVF RGNLEQVKEY QDLLTPLLHQ TTEGYPVVPK YYYVPADFVE CEKRNPGSQK 

       430        440        450        460        470        480 
RFPSNCGRDG KLFLWGQALY IIAKLLADEL ISPKDIDPVQ RFVPLQNQRN VSMRYSNQGP 

       490        500        510        520        530        540 
LENDLVVHVA LVAESQRLQV FLNTYGIQTQ TPQQVEPIQI WPQQELVKAY FHLGINEKLG 

       550        560        570        580        590        600 
LSGRPDRPIG CLGTSKIYRI LGKTVVCYPI IFDLSDFYMS QDVLLLIDDI KNALQFIKQY 

       610        620        630        640        650        660 
WKMHGRPLFL VLIREDNIRG SRFNPILDML AAFKKGIIGG VKVHVDRLQT LISGAVVEQL 

       670        680        690        700        710        720 
DFLRISDTEK LPEFKSFEEL EFPKHSKVKR QSSTADAPEA QHEPGITITE WKNKSTHEIL 

       730        740        750        760        770        780 
QKLNDCGCLA GQTILLGILL KREGPNFITM EGTVSDHIER VYRRAGSKKL WSVVRRAASL 

       790        800        810        820        830        840 
LNKVVDSLAP SITNVLVQGK QVTLGAFGHE EEVISNPLSP RVIKNIIYYK CNTHDEREAV 

       850        860        870        880        890        900 
IQQELVIHIG WIISNSPELF SGMLKIRIGW IIHAMEYELQ VRGGDKPAVD LYQLSPSEVK 

       910        920        930        940        950        960 
QLLLDILQPQ QSGRCWLNRR QIDGSLNRTP PEFYDRVWQI LERTPNGIVV AGKHLPQQPT 

       970        980        990       1000       1010       1020 
LSDMTMYEMN FSLLVEDMLG NIDQPKYRQI IVELLMVVSI VLERNPELEF QDKVDLDRLV 

      1030       1040       1050       1060       1070       1080 
KEAFHEFQKD ESRLKEIEKQ DDMTSFYNTP PLGKRGTCSY LTKVVMNSLL EGEVKPSNED 


SCLVS 

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References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC053105 mRNA. Translation: AAH53105.1.
CCDSCCDS22501.1.
RefSeqNP_955517.1. NM_199446.1.
XP_006530595.1. XM_006530532.1.
XP_006530596.1. XM_006530533.1.
XP_006530597.1. XM_006530534.1.
UniGeneMm.237296.

3D structure databases

ProteinModelPortalQ7TSH2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid221792. 1 interaction.
IntActQ7TSH2. 1 interaction.

PTM databases

PhosphoSiteQ7TSH2.

Proteomic databases

MaxQBQ7TSH2.
PaxDbQ7TSH2.
PRIDEQ7TSH2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879.
GeneID102093.
KEGGmmu:102093.
UCSCuc009mqj.1. mouse.

Organism-specific databases

CTD5257.
MGIMGI:97578. Phkb.

Phylogenomic databases

eggNOGNOG82518.
GeneTreeENSGT00520000055553.
HOGENOMHOG000231477.
HOVERGENHBG097309.
InParanoidQ7TSH2.
KOK07190.
OMALKGKYGF.
OrthoDBEOG73Z2SM.
PhylomeDBQ7TSH2.
TreeFamTF313970.

Enzyme and pathway databases

UniPathwayUPA00163.

Gene expression databases

ArrayExpressQ7TSH2.
BgeeQ7TSH2.
CleanExMM_PHKB.
GenevestigatorQ7TSH2.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR011613. Glyco_hydro_15.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERPTHR10749. PTHR10749. 1 hit.
PfamPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
ProtoNetSearch...

Other

NextBio355270.
PROQ7TSH2.
SOURCESearch...

Entry information

Entry nameKPBB_MOUSE
AccessionPrimary (citable) accession number: Q7TSH2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot