Q7TSH2 (KPBB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphorylase b kinase regulatory subunit beta
Short name=Phosphorylase kinase subunit beta

Gene names

Name:

Phkb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation By similarity.
Enzyme regulation	By phosphorylation of various serine residues By similarity.
Pathway	Glycan biosynthesis; glycogen metabolism.
Subunit structure	Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Post-translational modification	Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated By similarity.
Sequence similarities	Belongs to the phosphorylase b kinase regulatory chain family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Cellular component	Cell membrane Membrane
Ligand	Calmodulin-binding
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1085	1085	Phosphorylase b kinase regulatory subunit beta		PRO_0000057737
Regions
	Region	760 – 787	28	Calmodulin-binding Potential
	Region	912 – 943	32	Calmodulin-binding Potential
Amino acid modifications
	Modified residue	10	1	Phosphoserine By similarity
	Modified residue	19	1	Phosphoserine Ref.2
	Modified residue	693	1	Phosphoserine Ref.2
	Lipidation	1082	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TSH2 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8EC52645AD89FDF5	FASTA	1,085	123,889
	10 20 30 40 50 60 MANSPDAAFS SPALLRSGSV YEPLKSINLP RPDNETLWDK LDHYYRIVKS TMLMYQSPTT 70 80 90 100 110 120 GLFPTKTCGG EEKSKVHESL YCAAGAWALA LAYRRIDDDK GRTHELEHSA IKCMRGILYC 130 140 150 160 170 180 YMRQADKVQQ FKQDPRPTTC LHSVFSVHTG DELLSYEEYG HLQINAVSLF LLYLVEMISS 190 200 210 220 230 240 GLQIIYNTDE VSFIQNLVFC VERVYRVPDF GVWERGSKYN NGSTELHSSS VGLAKAALEA 250 260 270 280 290 300 INGFNLFGNQ GCSWSVIFVD LDAHNRNRQT LCSLLPRESR SHNTDAALLP CISYPAFALD 310 320 330 340 350 360 DEALFSQTLD KVIRKLKGKY GFKRFLRDGY RTPLEDPNRR YYKPAEIKLF DGIECEFPIF 370 380 390 400 410 420 FLYMMIDGVF RGNLEQVKEY QDLLTPLLHQ TTEGYPVVPK YYYVPADFVE CEKRNPGSQK 430 440 450 460 470 480 RFPSNCGRDG KLFLWGQALY IIAKLLADEL ISPKDIDPVQ RFVPLQNQRN VSMRYSNQGP 490 500 510 520 530 540 LENDLVVHVA LVAESQRLQV FLNTYGIQTQ TPQQVEPIQI WPQQELVKAY FHLGINEKLG 550 560 570 580 590 600 LSGRPDRPIG CLGTSKIYRI LGKTVVCYPI IFDLSDFYMS QDVLLLIDDI KNALQFIKQY 610 620 630 640 650 660 WKMHGRPLFL VLIREDNIRG SRFNPILDML AAFKKGIIGG VKVHVDRLQT LISGAVVEQL 670 680 690 700 710 720 DFLRISDTEK LPEFKSFEEL EFPKHSKVKR QSSTADAPEA QHEPGITITE WKNKSTHEIL 730 740 750 760 770 780 QKLNDCGCLA GQTILLGILL KREGPNFITM EGTVSDHIER VYRRAGSKKL WSVVRRAASL 790 800 810 820 830 840 LNKVVDSLAP SITNVLVQGK QVTLGAFGHE EEVISNPLSP RVIKNIIYYK CNTHDEREAV 850 860 870 880 890 900 IQQELVIHIG WIISNSPELF SGMLKIRIGW IIHAMEYELQ VRGGDKPAVD LYQLSPSEVK 910 920 930 940 950 960 QLLLDILQPQ QSGRCWLNRR QIDGSLNRTP PEFYDRVWQI LERTPNGIVV AGKHLPQQPT 970 980 990 1000 1010 1020 LSDMTMYEMN FSLLVEDMLG NIDQPKYRQI IVELLMVVSI VLERNPELEF QDKVDLDRLV 1030 1040 1050 1060 1070 1080 KEAFHEFQKD ESRLKEIEKQ DDMTSFYNTP PLGKRGTCSY LTKVVMNSLL EGEVKPSNED SCLVS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19 AND SER-693, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC053105 mRNA. Translation: AAH53105.1.
IPI	IPI00380735.
RefSeq	NP_955517.1. NM_199446.1.
UniGene	Mm.237296.
3D structure databases
ProteinModelPortal	Q7TSH2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TSH2. 1 interaction.
PTM databases
PhosphoSite	Q7TSH2.
Proteomic databases
PaxDb	Q7TSH2.
PRIDE	Q7TSH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879.
GeneID	102093.
KEGG	mmu:102093.
UCSC	uc009mqj.1. mouse.
Organism-specific databases
CTD	5257.
MGI	MGI:97578. Phkb.
Phylogenomic databases
eggNOG	NOG82518.
GeneTree	ENSGT00520000055553.
HOGENOM	HOG000231477.
HOVERGEN	HBG097309.
InParanoid	Q7TSH2.
KO	K07190.
OMA	SNQGPLE.
OrthoDB	EOG44BB1G.
Enzyme and pathway databases
UniPathway	UPA00163.
Gene expression databases
ArrayExpress	Q7TSH2.
Bgee	Q7TSH2.
CleanEx	MM_PHKB.
Genevestigator	Q7TSH2.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR011613. Glyco_hydro_15. IPR008734. PHK_A/B_su. [Graphical view]
PANTHER	PTHR10749. PTHR10749. 1 hit.
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
NextBio	355270.
SOURCE	Search...

Entry information

Entry name

KPBB_MOUSE

Accession

Primary (citable) accession number: Q7TSH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	October 1, 2003
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program