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Protein

Phosphorylase b kinase regulatory subunit beta

Gene

Phkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation (By similarity).By similarity

Enzyme regulationi

By phosphorylation of various serine residues.By similarity

Pathway: glycogen metabolism

This protein is involved in the pathway glycogen metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen metabolism and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiREACT_292811. Glycogen breakdown (glycogenolysis).
UniPathwayiUPA00163.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit beta
Short name:
Phosphorylase kinase subunit beta
Gene namesi
Name:Phkb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97578. Phkb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Phosphorylase b kinase regulatory subunit betaPRO_0000057737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Modified residuei693 – 6931PhosphoserineBy similarity
Lipidationi1082 – 10821S-farnesyl cysteineBy similarity

Post-translational modificationi

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ7TSH2.
PaxDbiQ7TSH2.
PRIDEiQ7TSH2.

PTM databases

PhosphoSiteiQ7TSH2.

Expressioni

Gene expression databases

BgeeiQ7TSH2.
CleanExiMM_PHKB.
ExpressionAtlasiQ7TSH2. baseline and differential.
GenevisibleiQ7TSH2. MM.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin (By similarity).By similarity

Protein-protein interaction databases

BioGridi221792. 1 interaction.
IntActiQ7TSH2. 1 interaction.
STRINGi10090.ENSMUSP00000050788.

Structurei

3D structure databases

ProteinModelPortaliQ7TSH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni760 – 78728Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni912 – 94332Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG82518.
GeneTreeiENSGT00520000055553.
HOGENOMiHOG000231477.
HOVERGENiHBG097309.
InParanoidiQ7TSH2.
KOiK07190.
OMAiLKGKYGF.
OrthoDBiEOG73Z2SM.
PhylomeDBiQ7TSH2.
TreeFamiTF313970.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR011613. Glyco_hydro_15/PHK.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 1 hit.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7TSH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSPDAAFS SPALLRSGSV YEPLKSINLP RPDNETLWDK LDHYYRIVKS
60 70 80 90 100
TMLMYQSPTT GLFPTKTCGG EEKSKVHESL YCAAGAWALA LAYRRIDDDK
110 120 130 140 150
GRTHELEHSA IKCMRGILYC YMRQADKVQQ FKQDPRPTTC LHSVFSVHTG
160 170 180 190 200
DELLSYEEYG HLQINAVSLF LLYLVEMISS GLQIIYNTDE VSFIQNLVFC
210 220 230 240 250
VERVYRVPDF GVWERGSKYN NGSTELHSSS VGLAKAALEA INGFNLFGNQ
260 270 280 290 300
GCSWSVIFVD LDAHNRNRQT LCSLLPRESR SHNTDAALLP CISYPAFALD
310 320 330 340 350
DEALFSQTLD KVIRKLKGKY GFKRFLRDGY RTPLEDPNRR YYKPAEIKLF
360 370 380 390 400
DGIECEFPIF FLYMMIDGVF RGNLEQVKEY QDLLTPLLHQ TTEGYPVVPK
410 420 430 440 450
YYYVPADFVE CEKRNPGSQK RFPSNCGRDG KLFLWGQALY IIAKLLADEL
460 470 480 490 500
ISPKDIDPVQ RFVPLQNQRN VSMRYSNQGP LENDLVVHVA LVAESQRLQV
510 520 530 540 550
FLNTYGIQTQ TPQQVEPIQI WPQQELVKAY FHLGINEKLG LSGRPDRPIG
560 570 580 590 600
CLGTSKIYRI LGKTVVCYPI IFDLSDFYMS QDVLLLIDDI KNALQFIKQY
610 620 630 640 650
WKMHGRPLFL VLIREDNIRG SRFNPILDML AAFKKGIIGG VKVHVDRLQT
660 670 680 690 700
LISGAVVEQL DFLRISDTEK LPEFKSFEEL EFPKHSKVKR QSSTADAPEA
710 720 730 740 750
QHEPGITITE WKNKSTHEIL QKLNDCGCLA GQTILLGILL KREGPNFITM
760 770 780 790 800
EGTVSDHIER VYRRAGSKKL WSVVRRAASL LNKVVDSLAP SITNVLVQGK
810 820 830 840 850
QVTLGAFGHE EEVISNPLSP RVIKNIIYYK CNTHDEREAV IQQELVIHIG
860 870 880 890 900
WIISNSPELF SGMLKIRIGW IIHAMEYELQ VRGGDKPAVD LYQLSPSEVK
910 920 930 940 950
QLLLDILQPQ QSGRCWLNRR QIDGSLNRTP PEFYDRVWQI LERTPNGIVV
960 970 980 990 1000
AGKHLPQQPT LSDMTMYEMN FSLLVEDMLG NIDQPKYRQI IVELLMVVSI
1010 1020 1030 1040 1050
VLERNPELEF QDKVDLDRLV KEAFHEFQKD ESRLKEIEKQ DDMTSFYNTP
1060 1070 1080
PLGKRGTCSY LTKVVMNSLL EGEVKPSNED SCLVS
Length:1,085
Mass (Da):123,889
Last modified:October 1, 2003 - v1
Checksum:i8EC52645AD89FDF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053105 mRNA. Translation: AAH53105.1.
CCDSiCCDS22501.1.
RefSeqiNP_955517.1. NM_199446.1.
XP_006530595.1. XM_006530532.2.
XP_006530596.1. XM_006530533.2.
XP_006530597.1. XM_006530534.2.
UniGeneiMm.237296.

Genome annotation databases

EnsembliENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879.
GeneIDi102093.
KEGGimmu:102093.
UCSCiuc009mqj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053105 mRNA. Translation: AAH53105.1.
CCDSiCCDS22501.1.
RefSeqiNP_955517.1. NM_199446.1.
XP_006530595.1. XM_006530532.2.
XP_006530596.1. XM_006530533.2.
XP_006530597.1. XM_006530534.2.
UniGeneiMm.237296.

3D structure databases

ProteinModelPortaliQ7TSH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221792. 1 interaction.
IntActiQ7TSH2. 1 interaction.
STRINGi10090.ENSMUSP00000050788.

PTM databases

PhosphoSiteiQ7TSH2.

Proteomic databases

MaxQBiQ7TSH2.
PaxDbiQ7TSH2.
PRIDEiQ7TSH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053771; ENSMUSP00000050788; ENSMUSG00000036879.
GeneIDi102093.
KEGGimmu:102093.
UCSCiuc009mqj.1. mouse.

Organism-specific databases

CTDi5257.
MGIiMGI:97578. Phkb.

Phylogenomic databases

eggNOGiNOG82518.
GeneTreeiENSGT00520000055553.
HOGENOMiHOG000231477.
HOVERGENiHBG097309.
InParanoidiQ7TSH2.
KOiK07190.
OMAiLKGKYGF.
OrthoDBiEOG73Z2SM.
PhylomeDBiQ7TSH2.
TreeFamiTF313970.

Enzyme and pathway databases

UniPathwayiUPA00163.
ReactomeiREACT_292811. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi355270.
PROiQ7TSH2.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TSH2.
CleanExiMM_PHKB.
ExpressionAtlasiQ7TSH2. baseline and differential.
GenevisibleiQ7TSH2. MM.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR011613. Glyco_hydro_15/PHK.
IPR008734. PHK_A/B_su.
[Graphical view]
PANTHERiPTHR10749. PTHR10749. 1 hit.
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiKPBB_MOUSE
AccessioniPrimary (citable) accession number: Q7TSH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2003
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.