Q7TSG2 (CTDP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase
EC=3.1.3.16

Alternative name(s):
TFIIF-associating CTD phosphatase

Gene names

Name:	Ctdp1
Synonyms:	Fcp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	960 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Homodimer. Interacts with GTF2F1 By similarity. Interacts with WDR77, SNRPB and SNRNP70 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Midbody By similarity. Note: Found at centrosomes in prometaphase, at spindle and spindle poles in metaphase and at spindle midzone and midbody in anaphase and telophase-G1 respectively By similarity.
Post-translational modification	Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1 By similarity.
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: Compara centrosome Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW midbody Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle midzone Inferred from sequence or structural similarity. Source: UniProtKB spindle pole Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	CTD phosphatase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TSG2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TSG2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-788: Missing.
     789-796: NHGEPSSF → MSRIILVV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 960	960	RNA polymerase II subunit A C-terminal domain phosphatase		PRO_0000212565
Regions
	Domain	178 – 341	164	FCP1 homology
	Domain	619 – 718	100	BRCT
	Compositional bias	414 – 417	4	Poly-Ser
	Compositional bias	568 – 572	5	Poly-Glu
	Compositional bias	573 – 578	6	Poly-Asp
Amino acid modifications
	Modified residue	502	1	Phosphoserine Ref.3
	Modified residue	664	1	Phosphoserine By similarity
	Modified residue	770	1	N6-acetyllysine By similarity
	Modified residue	860	1	Phosphoserine Ref.3
	Modified residue	863	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	1 – 788	788	Missing in isoform 2.		VSP_009866
	Alternative sequence	789 – 796	8	NHGEPSSF → MSRIILVV in isoform 2.		VSP_009867
Experimental info
	Sequence conflict	759 – 761	3	PEV → GTR in AAH52934. Ref.2
	Sequence conflict	897	1	V → I in AAH52934. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7D1EB42C6D15C95C	FASTA	960	104,554
	10 20 30 40 50 60 MEAPPAAGVP TECTPAVAGA EVRCPGPTPL RLLEWKVAAG ATVRIGSVLA VCETAASAQP 70 80 90 100 110 120 AGPAPARAAS GGCVRAARTE RRLRSERAGV VRELCAQPGQ VVAPGALLVR LEGCSHPVVM 130 140 150 160 170 180 KGLCAECGQD LTQLQSKNGR QQVPLSTATV SMVHSVPELM VSSEQAEKLG REDQQRLHRN 190 200 210 220 230 240 RKLVLMVDLD QTLIHTTEQH CPQMSNKGIF HFQLGRGEPM LHTRLRPHCK DFLEKIAKLY 250 260 270 280 290 300 ELHVFTFGSR LYAHTIAGFL DPEKKLFSHR ILSRDECIDP FSKTGNLRNL FPCGDSMVCI 310 320 330 340 350 360 IDDREDVWKF APNLITVKKY VYFPGTGDVN APPAARETQA RRKVNHSSKG GDALEQALSV 370 380 390 400 410 420 RDPEDGRPAP GVEHSNGLGK ASRELNGGEA VPGVFPSKAD EKEAWPLTRA SPASSSSGHE 430 440 450 460 470 480 PTEAPELPVS CEWDGRTTPG VQPTQGDAAT QDLDFDLSSD SESSESSSRS EGQRAPAPQE 490 500 510 520 530 540 RTKAAPEHSG PQDTSGGRAA ASPLGESGPS IHPHDKGSDL DTQEEGERDS LCGLGNGSVD 550 560 570 580 590 600 RKEAETESQN SEQSGVTAGE SLDQSVGEEE EEDTDDDDHL IHLEEILVRV HTDYYTKYDR 610 620 630 640 650 660 YLNKELEEAP DIRKIVPELK SKVLADVAVI FSGLHPTNFP VEKTREHYHA TALGAKVLTQ 670 680 690 700 710 720 LVLSPDAPDR ATHLIAARAG TEKVRQAQEC KHLHVVSPDW LWSCLERWDK VEEQLFPLID 730 740 750 760 770 780 DDTRTHRDNS PAVFPDRHSV LPTALFHPTP IHSKAHPGPE VRIYDSNTGK LIRMGPQGSA 790 800 810 820 830 840 PAPSSAPLNH GEPSSFRAVQ PHQQQMFGEE LPESQDGEQP GPARRKRQPS MSEAMPLYTL 850 860 870 880 890 900 CKEDLESMDK EVDDILGEGS DDSDIEKKKP EDQDNEQERA PKPRKPRAPG IRREQPVGLP 910 920 930 940 950 960 SSGERSTPGM RGPRGHKRKL NEEDAASESS GESSNDDEEG SSSEADEMAA ALEAELNDLM
	Isoform 2 [UniParc]. Checksum: E3243D9122FC5811	FASTA	172	19,043
	10 20 30 40 50 60 MSRIILVVRA VQPHQQQMFG EELPESQDGE QPGPARRKRQ PSMSEAMPLY TLCKEDLESM 70 80 90 100 110 120 DKEVDDILGE GSDDSDIEKK KPEDQDNEQE RAPKPRKPRA PGIRREQPVG LPSSGERSTP 130 140 150 160 170 GMRGPRGHKR KLNEEDAASE SSGESSNDDE EGSSSEADEM AAALEAELND LM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo and Lung.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502; SER-860 AND SER-863, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK016213 mRNA. Translation: BAB30150.1. BC052934 mRNA. Translation: AAH52934.1. BC053435 mRNA. Translation: AAH53435.1.
IPI	IPI00338904. IPI00411156.
RefSeq	NP_080571.2. NM_026295.2.
UniGene	Mm.312893.
3D structure databases
ProteinModelPortal	Q7TSG2.
SMR	Q7TSG2. Positions 168-370, 547-718.
ModBase	Search...
PTM databases
PhosphoSite	Q7TSG2.
Proteomic databases
PaxDb	Q7TSG2.
PRIDE	Q7TSG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036229; ENSMUSP00000038938; ENSMUSG00000033323.
GeneID	67655.
KEGG	mmu:67655.
UCSC	uc008ftb.1. mouse.
Organism-specific databases
CTD	9150.
MGI	MGI:1926953. Ctdp1.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00390000015641.
HOGENOM	HOG000112039.
HOVERGEN	HBG051213.
InParanoid	Q7TSG2.
KO	K15732.
OMA	EAPDIRK.
OrthoDB	EOG4HMJ8T.
Gene expression databases
ArrayExpress	Q7TSG2.
Bgee	Q7TSG2.
Genevestigator	Q7TSG2.
GermOnline	ENSMUSG00000033323. Mus musculus.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001357. BRCT_dom. IPR015388. FCP1_C. IPR011947. FCP1_euk. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF09309. FCP1_C. 1 hit. PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	325156.
SOURCE	Search...

Entry information

Entry name

CTDP1_MOUSE

Accession

Primary (citable) accession number: Q7TSG2
Secondary accession number(s): Q7TSS7, Q9D4S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program