Reviewed, UniProtKB/Swiss-Prot Q7TSG2 (CTDP1_MOUSE)

Names and origin

Protein names

Recommended name:
    RNA polymerase II subunit A C-terminal domain phosphatase
    EC=3.1.3.16
Alternative name(s):
    TFIIF-associating CTD phosphatase

Gene names

Name:	Ctdp1
Synonyms:	Fcp1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	960 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Homodimer. Interacts with GTF2F1 By similarity. Interacts with WDR77, SNRPB and SNRNP70 By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1 By similarity. Ref.3
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TSG2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TSG2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-788: Missing.
     789-796: NHGEPSSF → MSRIILVV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 960	960	RNA polymerase II subunit A C-terminal domain phosphatase		PRO_0000212565
Regions
	Domain	178 – 341	164	FCP1 homology
	Domain	619 – 718	100	BRCT
	Compositional bias	414 – 417	4	Poly-Ser
	Compositional bias	568 – 572	5	Poly-Glu
	Compositional bias	573 – 578	6	Poly-Asp
Amino acid modifications
	Modified residue	502	1	Phosphoserine Ref.3
	Modified residue	664	1	Phosphoserine By similarity
	Modified residue	730	1	Phosphoserine By similarity
	Modified residue	770	1	N6-acetyllysine By similarity
	Modified residue	860	1	Phosphoserine Ref.3
	Modified residue	863	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	1 – 788	788	Missing in isoform 2.		VSP_009866
	Alternative sequence	789 – 796	8	NHGEPSSF → MSRIILVV in isoform 2.		VSP_009867
Experimental info
	Sequence conflict	759 – 761	3	PEV → GTR in AAH52934. Ref.2
	Sequence conflict	897	1	V → I in AAH52934. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7D1EB42C6D15C95C	FASTA	960	104,554
	10 20 30 40 50 60 MEAPPAAGVP TECTPAVAGA EVRCPGPTPL RLLEWKVAAG ATVRIGSVLA VCETAASAQP 70 80 90 100 110 120 AGPAPARAAS GGCVRAARTE RRLRSERAGV VRELCAQPGQ VVAPGALLVR LEGCSHPVVM 130 140 150 160 170 180 KGLCAECGQD LTQLQSKNGR QQVPLSTATV SMVHSVPELM VSSEQAEKLG REDQQRLHRN 190 200 210 220 230 240 RKLVLMVDLD QTLIHTTEQH CPQMSNKGIF HFQLGRGEPM LHTRLRPHCK DFLEKIAKLY 250 260 270 280 290 300 ELHVFTFGSR LYAHTIAGFL DPEKKLFSHR ILSRDECIDP FSKTGNLRNL FPCGDSMVCI 310 320 330 340 350 360 IDDREDVWKF APNLITVKKY VYFPGTGDVN APPAARETQA RRKVNHSSKG GDALEQALSV 370 380 390 400 410 420 RDPEDGRPAP GVEHSNGLGK ASRELNGGEA VPGVFPSKAD EKEAWPLTRA SPASSSSGHE 430 440 450 460 470 480 PTEAPELPVS CEWDGRTTPG VQPTQGDAAT QDLDFDLSSD SESSESSSRS EGQRAPAPQE 490 500 510 520 530 540 RTKAAPEHSG PQDTSGGRAA ASPLGESGPS IHPHDKGSDL DTQEEGERDS LCGLGNGSVD 550 560 570 580 590 600 RKEAETESQN SEQSGVTAGE SLDQSVGEEE EEDTDDDDHL IHLEEILVRV HTDYYTKYDR 610 620 630 640 650 660 YLNKELEEAP DIRKIVPELK SKVLADVAVI FSGLHPTNFP VEKTREHYHA TALGAKVLTQ 670 680 690 700 710 720 LVLSPDAPDR ATHLIAARAG TEKVRQAQEC KHLHVVSPDW LWSCLERWDK VEEQLFPLID 730 740 750 760 770 780 DDTRTHRDNS PAVFPDRHSV LPTALFHPTP IHSKAHPGPE VRIYDSNTGK LIRMGPQGSA 790 800 810 820 830 840 PAPSSAPLNH GEPSSFRAVQ PHQQQMFGEE LPESQDGEQP GPARRKRQPS MSEAMPLYTL 850 860 870 880 890 900 CKEDLESMDK EVDDILGEGS DDSDIEKKKP EDQDNEQERA PKPRKPRAPG IRREQPVGLP 910 920 930 940 950 960 SSGERSTPGM RGPRGHKRKL NEEDAASESS GESSNDDEEG SSSEADEMAA ALEAELNDLM
	Isoform 2. Checksum: E3243D9122FC5811	FASTA	172	19,043
	10 20 30 40 50 60 MSRIILVVRA VQPHQQQMFG EELPESQDGE QPGPARRKRQ PSMSEAMPLY TLCKEDLESM 70 80 90 100 110 120 DKEVDDILGE GSDDSDIEKK KPEDQDNEQE RAPKPRKPRA PGIRREQPVG LPSSGERSTP 130 140 150 160 170 GMRGPRGHKR KLNEEDAASE SSGESSNDDE EGSSSEADEM AAALEAELND LM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo and Lung.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502; SER-860 AND SER-863, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK016213 mRNA. Translation: BAB30150.1. BC052934 mRNA. Translation: AAH52934.1. BC053435 mRNA. Translation: AAH53435.1.
IPI	IPI00338904. IPI00411156.
RefSeq	NP_080571.2.
UniGene	Mm.312893
3D structure databases
SMR	Q7TSG2. Positions 21-113, 177-321, 607-720.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TSG2.
PTM databases
PhosphoSite	Q7TSG2.
Proteomic databases
PRIDE	Q7TSG2.
Genome annotation databases
Ensembl	ENSMUST00000036229; ENSMUSP00000038938; ENSMUSG00000033323; Mus musculus. [Genome view] ENSMUST00000114707; ENSMUSP00000110355; ENSMUSG00000033323; Mus musculus. [Genome view]
GeneID	67655.
KEGG	mmu:67655.
UCSC	uc008ftb.1. mouse.
Organism-specific databases
CTD	67655.
MGI	MGI:1926953. Ctdp1.
Phylogenomic databases
HOGENOM	HBG403000.
HOVERGEN	Q7TSG2.
InParanoid	Q7TSG2.
OMA	THLIAAR.
OrthoDB	EOG92Z78N.
PhylomeDB	Q7TSG2.
Enzyme and pathway databases
BRENDA	3.1.3.16. 244.
Gene expression databases
ArrayExpress	Q7TSG2.
Bgee	Q7TSG2.
Genevestigator	Q7TSG2.
GermOnline	ENSMUSG00000033323. Mus musculus.
Family and domain databases
InterPro	IPR001357. BRCT. IPR015388. FCP1_C. IPR011947. FCP1_euk. IPR004274. NIF. IPR011053. Single_hybrid_motif. [Graphical view]
Pfam	PF09309. FCP1_C. 1 hit. PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	325156.
SOURCE	Search...

Entry information

Entry name

CTDP1_MOUSE

Accession

Primary (citable) accession number: Q7TSG2
Secondary accession number(s): Q7TSS7, Q9D4S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)