Vesicular glutamate transporter 3 - Rattus norvegicus (Rat)

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Q7TSF2 (VGLU3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vesicular glutamate transporter 3
Short name=VGluT3

Alternative name(s):
Solute carrier family 17 member 8

Gene names

Name:	Slc17a8
Synonyms:	Vglut3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.1 Ref.2
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Membrane; Multi-pass membrane protein Potential. Cell junction › synapse › synaptosome Ref.2 Ref.7.
Tissue specificity	Expressed in brain, kidney and liver. Expressed within the amygdala, brainstem, cerberal cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, hypothalamus, retina, striatum and ventral spinal cord. Expressed within neurons of the caudate-putamen, olfactory tubercle, nucleus accumbens, hippocampus, interpeduncular nucleus and dorsal and medial raphe nuclei. Expressed in inner hair cells of the ear. Expressed at synaptic terminals within the lateral superior olive (LSO), a nucleus of the mammalian sound localization system, and in the medial nucleus of the trapezoid body (MNTB), which provides inhibitory input to the LSO. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.8
Sequence similarities	Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
Biological process	Hearing Ion transport Neurotransmitter transport Sodium transport Symport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 588

588

Vesicular glutamate transporter 3

PRO_0000331616

Regions

Topological domain

1 – 76

Cytoplasmic Potential

Transmembrane

77 – 97

Helical; Potential

Topological domain

98 – 130

Vesicular Potential

Transmembrane

131 – 151

Helical; Potential

Topological domain

152 – 153

Cytoplasmic Potential

Transmembrane

154 – 174

Helical; Potential

Topological domain

175 – 182

Vesicular Potential

Transmembrane

183 – 203

Helical; Potential

Topological domain

204 – 221

Cytoplasmic Potential

Transmembrane

222 – 242

Helical; Potential

Topological domain

243 – 249

Vesicular Potential

Transmembrane

250 – 270

Helical; Potential

Topological domain

271 – 314

Cytoplasmic Potential

Transmembrane

315 – 335

Helical; Potential

Topological domain

336 – 353

Vesicular Potential

Transmembrane

354 – 374

Helical; Potential

Topological domain

375 – 390

Cytoplasmic Potential

Transmembrane

391 – 411

Helical; Potential

Topological domain

412 – 413

Vesicular Potential

Transmembrane

414 – 434

Helical; Potential

Topological domain

435 – 447

Cytoplasmic Potential

Transmembrane

448 – 468

Helical; Potential

Topological domain

469 – 481

Vesicular Potential

Transmembrane

482 – 502

Helical; Potential

Topological domain

503 – 585

Cytoplasmic Potential

Amino acid modifications

Glycosylation

106

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

N → K in AAM50094. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q7TSF2 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 9644C021D94286B5
Show »

FASTA

588

64,755

        10         20         30         40         50         60 
MPFNAFDTFK EKILKPGKEG VKNAVGDSLG ILQRKLDGTN EEGDAIELSE EGRPVQTSRA 

        70         80         90        100        110        120 
RAPVCDCSCC GIPKRYIIAV MSGLGFCISF GIRCNLGVAI VEMVNNSTVY VDGKPEIQTA 

       130        140        150        160        170        180 
QFNWDPETVG LIHGSFFWGY IVTQIPGGFI SNKFAANRVF GAAIFLTSTL NMFIPSAARV 

       190        200        210        220        230        240 
HYGCVMCVRI LQGLVEGVTY PACHGMWSKW APPLERSRLA TTSFCGSYAG AVVAMPLAGV 

       250        260        270        280        290        300 
LVQYIGWASV FYIYGMFGII WYMFWLLQAY ECPAVHPTIS NEERTYIETS IGEGANLASL 

       310        320        330        340        350        360 
SKFNTPWRRF FTSLPVYAII VANFCRSWTF YLLLISQPAY FEEVFGFAIS KVGLLSAVPH 

       370        380        390        400        410        420 
MVMTIVVPIG GQLADYLRSR KILTTTAVRK IMNCGGFGME ATLLLVVGFS HTKGVAISFL 

       430        440        450        460        470        480 
VLAVGFSGFA ISGFNVNHLD IAPRYASILM GISNGVGTLS GMVCPLIVGA MTKHKTREEW 

       490        500        510        520        530        540 
QNVFLIAALV HYSGVIFYGV FASGEKQDWA DPENLSEEKC GIIDQDELAE ETELNHEAFV 

       550        560        570        580 
SPRKKMSYGA TTQNCEVQKT DRRQQRESAF EGEEPLSYQN EEDFSETS

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References

[1]	"A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons." Gras C., Herzog E., Bellenchi G.C., Bernard V., Ravassard P., Pohl M., Gasnier B., Giros B., El Mestikawy S. J. Neurosci. 22:5442-5451(2002) [PubMed: 12097496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The identification of vesicular glutamate transporter 3 suggests novel modes of signaling by glutamate." Fremeau R.T. Jr., Burman J., Qureshi T., Tran C.H., Proctor J., Johnson J., Zhang H., Sulzer D., Copenhagen D.R., Storm-Mathisen J., Reimer R.J., Chaudhry F.A., Edwards R.H. Proc. Natl. Acad. Sci. U.S.A. 99:14488-14493(2002) [PubMed: 12388773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D. J. Biol. Chem. 277:50734-50748(2002) [PubMed: 12384506] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Localization of VGLUT3, the vesicular glutamate transporter type 3, in the rat brain." Herzog E., Gilchrist J., Gras C., Muzerelle A., Ravassard P., Giros B., Gaspar P., El Mestikawy S. Neuroscience 123:983-1002(2004) [PubMed: 14751290] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Inhibitory synapses in the developing auditory system are glutamatergic." Gillespie D.C., Kim G., Kandler K. Nat. Neurosci. 8:332-338(2005) [PubMed: 15746915] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Molecular anatomy of a trafficking organelle." Takamori S., Holt M., Stenius K., Lemke E.A., Groenborg M., Riedel D., Urlaub H., Schenck S., Bruegger B., Ringler P., Mueller S.A., Rammner B., Graeter F., Hub J.S., De Groot B.L., Mieskes G., Moriyama Y., Klingauf J. Jahn R. Cell 127:831-846(2006) [PubMed: 17110340] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone." Gras C., Amilhon B., Lepicard E.M., Poirel O., Vinatier J., Herbin M., Dumas S., Tzavara E.T., Wade M.R., Nomikos G.G., Hanoun N., Saurini F., Kemel M.-L., Gasnier B., Giros B., Mestikawy S.E. Nat. Neurosci. 11:292-300(2008) [PubMed: 18278042] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3." Seal R.P., Akil O., Yi E., Weber C.M., Grant L., Yoo J., Clause A., Kandler K., Noebels J.L., Glowatzki E., Lustig L.R., Edwards R.H. Neuron 57:263-275(2008) [PubMed: 18215623] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ491795 mRNA. Translation: CAD37138.1. AY117026 mRNA. Translation: AAM50094.1.
IPI	IPI00200411.
RefSeq	NP_714947.1. NM_153725.1.
UniGene	Rn.84876.
3D structure databases
ProteinModelPortal	Q7TSF2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TSF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010207; ENSRNOP00000010207; ENSRNOG00000007581.
GeneID	266767.
KEGG	rno:266767.
Organism-specific databases
CTD	246213.
RGD	628870. Slc17a8.
Phylogenomic databases
eggNOG	roNOG05301.
GeneTree	ENSGT00600000084103.
HOVERGEN	HBG008834.
InParanoid	Q7TSF2.
OMA	YMFWLLQ.
OrthoDB	EOG4XWFXC.
PhylomeDB	Q7TSF2.
Gene expression databases
Genevestigator	Q7TSF2.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
KO	K12302.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624626.

Entry information

Entry name

VGLU3_RAT

Accession

Primary (citable) accession number: Q7TSF2
Secondary accession number(s): Q8K1Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	November 16, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents