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Protein

HCLS1-associated protein X-1

Gene

Hax1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
HCLS1-associated protein X-1
Alternative name(s):
HS1-associating protein X-1
Short name:
HAX-1
HS1-binding protein 1
Short name:
HSP1BP-1
Gene namesi
Name:Hax1
Synonyms:Hs1bp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727960. Hax1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • clathrin-coated vesicle Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrion Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB-SubCell
  • sarcoplasmic reticulum Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 278277HCLS1-associated protein X-1PRO_0000313801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei126 – 1272Cleavage; by caspase-3By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ7TSE9.
PRIDEiQ7TSE9.

Expressioni

Tissue specificityi

Present in striated muscles (at protein level).1 Publication

Developmental stagei

Abundant in hindlimb and cardiac muscles throughout embryogenesis (at protein level).1 Publication

Interactioni

Subunit structurei

Directly associates with HCLS1/HS1, through binding to its N-terminal region. Interacts with CTTN. Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7 (By similarity). Interacts with ABCB1, ABCB4 and ABCB11. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Abcb11O701275EBI-930005,EBI-930036
Abcb4Q082013EBI-930005,EBI-929988
Mdr1aQ6PSM02EBI-930005,EBI-930055

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ7TSE9. 4 interactions.
STRINGi10116.ENSRNOP00000067159.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 4342Required for localization in mitochondriaAdd
BLAST
Regioni113 – 278166Involved in HCLS1 bindingBy similarityAdd
BLAST
Regioni174 – 20532Involved in CASP9 bindingBy similarityAdd
BLAST
Regioni175 – 24672Involved in GNA13 bindingBy similarityAdd
BLAST
Regioni182 – 27897Required for localization in sarcoplasmic reticulumAdd
BLAST
Regioni183 – 27896Involved in PKD2 bindingBy similarityAdd
BLAST
Regioni202 – 24443Involved in ATP2A2 bindingBy similarityAdd
BLAST
Regioni202 – 22423Involved in PLN bindingBy similarityAdd
BLAST
Regioni269 – 27810Required for ITGB6 bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 4213Asp/Glu-rich (highly acidic)Add
BLAST

Sequence similaritiesi

Belongs to the HAX1 family.Curated

Phylogenomic databases

HOVERGENiHBG002991.
InParanoidiQ7TSE9.
KOiK16220.
PhylomeDBiQ7TSE9.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDEDDEEE EDSGAWGRES
60 70 80 90 100
YAFDGFHPTE EFGFSFSPRG GMRFHGNFGF DDLVRDFNSI FSEMGAWTLP
110 120 130 140 150
SHSPELPGPE SETPGVRLRE GQTLRDSMLK YPDSHQPRIF EGVLESHAKP
160 170 180 190 200
ESSKPAPDWG SQGPFHRLDD TWPVSPHSRA REDKDLDSQV SQEGLGPLLQ
210 220 230 240 250
PQPKSYFKSI SVTKITKPDG TVEEHRTVVD SEGRRETTVT HQEAHDSSRS
260 270
DPDPPRSSAL DDPFSILDLL LGRWFRSR
Length:278
Mass (Da):31,448
Last modified:October 1, 2003 - v1
Checksum:i0B49E32FF89CD975
GO
Isoform 2 (identifier: Q7TSE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-104: Missing.

Show »
Length:217
Mass (Da):24,572
Checksum:i822F48152513778E
GO
Isoform 3 (identifier: Q7TSE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSVFDLFRGFFGFPGPRS → MQQGPERRKQWGSGKEDREQVIG

Show »
Length:283
Mass (Da):32,084
Checksum:iE7BB60A4895ECB21
GO
Isoform 5 (identifier: Q7TSE9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:252
Mass (Da):28,488
Checksum:iD3163FE42C27D946
GO
Isoform 6 (identifier: Q7TSE9-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     44-104: Missing.

Show »
Length:191
Mass (Da):21,611
Checksum:iF0C0BA00B2450DA2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2626Missing in isoform 5 and isoform 6. 1 PublicationVSP_030138Add
BLAST
Alternative sequencei1 – 1818MSVFD…PGPRS → MQQGPERRKQWGSGKEDREQ VIG in isoform 3. 1 PublicationVSP_030137Add
BLAST
Alternative sequencei44 – 10461Missing in isoform 2 and isoform 6. 1 PublicationVSP_030139Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA. Translation: AAP44974.1.
AY291063 mRNA. Translation: AAP44975.1.
AY291064 mRNA. Translation: AAP44976.1.
AY919342 mRNA. Translation: AAX18866.1.
AY919343 mRNA. Translation: AAX18867.1.
DQ286293 Genomic DNA. Translation: ABB91376.1.
RefSeqiNP_853658.1. NM_181627.2. [Q7TSE9-1]
UniGeneiRn.185269.

Genome annotation databases

GeneIDi291202.
KEGGirno:291202.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA. Translation: AAP44974.1.
AY291063 mRNA. Translation: AAP44975.1.
AY291064 mRNA. Translation: AAP44976.1.
AY919342 mRNA. Translation: AAX18866.1.
AY919343 mRNA. Translation: AAX18867.1.
DQ286293 Genomic DNA. Translation: ABB91376.1.
RefSeqiNP_853658.1. NM_181627.2. [Q7TSE9-1]
UniGeneiRn.185269.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TSE9. 4 interactions.
STRINGi10116.ENSRNOP00000067159.

Proteomic databases

PaxDbiQ7TSE9.
PRIDEiQ7TSE9.

Protocols and materials databases

DNASUi291202.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi291202.
KEGGirno:291202.

Organism-specific databases

CTDi10456.
RGDi727960. Hax1.

Phylogenomic databases

HOVERGENiHBG002991.
InParanoidiQ7TSE9.
KOiK16220.
PhylomeDBiQ7TSE9.

Miscellaneous databases

NextBioi632255.
PROiQ7TSE9.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification and expression analysis of alternative splice variants of the rat Hax-1 gene."
    Grzybowska E.A., Sarnowska E., Konopinski R., Wilczynska A., Sarnowski T.J., Siedlecki J.A.
    Gene 371:84-92(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 5 AND 6).
    Strain: Lewis.
    Tissue: Testis.
  2. "Identification of HAX-1 as a protein that binds bile salt export protein and regulates its abundance in the apical membrane of Madin-Darby canine kidney cells."
    Ortiz D.F., Moseley J., Calderon G., Swift A.L., Li S., Arias I.M.
    J. Biol. Chem. 279:32761-32770(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABCB1; ABCB4; ABCB11 AND CTTN, SUBCELLULAR LOCATION, FUNCTION.
  3. "HAX-1: a multifaceted antiapoptotic protein localizing in the mitochondria and the sarcoplasmic reticulum of striated muscle cells."
    Yap S.V., Vafiadaki E., Strong J., Kontrogianni-Konstantopoulos A.
    J. Mol. Cell. Cardiol. 48:1266-1279(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiHAX1_RAT
AccessioniPrimary (citable) accession number: Q7TSE9
Secondary accession number(s): Q5D1N3
, Q5D1N4, Q7TSE7, Q7TSE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: November 11, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.