Reviewed, UniProtKB/Swiss-Prot Q7TSE9 (HAX1_RAT)

Names and origin

Protein names

Recommended name:
    HCLS1-associated protein X-1
Alternative name(s):
    HS1-associating protein X-1
      Short name=HAX-1
    HS1-binding protein 1

Gene names

Name:	Hax1
Synonyms:	Hs1bp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function in promoting cell survival By similarity.
Subunit structure	Directly associates with HCLS1/HS1, through binding to its N-terminal region. May also associate with cortactin/EMS1 in non-lymphoid cells By similarity.
Subcellular location	Mitochondrion By similarity. Endoplasmic reticulum By similarity. Nucleus membrane By similarity. Note: Predominantly mitochondrial, but also to a lesser extent in endoplasmic reticulum and nuclear envelope By similarity.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
PTM	Acetylation
Gene Ontology (GO)
Cellular component	clathrin-coated vesicle Inferred from direct assay. Source: RGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Abcb11	O70127	3	EBI-930005,EBI-930036
Abcb1a	Q6PSM0	1	EBI-930005,EBI-930055
Abcb4	Q08201	3	EBI-930005,EBI-929988
Cttn	O70420	1	EBI-930005,EBI-922114

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TSE9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TSE9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     44-104: Missing.

Isoform 3 (identifier: Q7TSE9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSVFDLFRGFFGFPGPRS → MQQGPERRKQWGSGKEDREQVIG

Isoform 5 (identifier: Q7TSE9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Isoform 6 (identifier: Q7TSE9-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     44-104: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 278	277	HCLS1-associated protein X-1		PRO_0000313801
Regions
	Region	113 – 278	166	Involved in HCLS1 binding By similarity
	Compositional bias	30 – 42	13	Asp/Glu-rich (highly acidic)
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
Natural variations
	Alternative sequence	1 – 26	26	Missing in isoform 5 and isoform 6.		VSP_030138
	Alternative sequence	1 – 18	18	MSVFD…PGPRS → MQQGPERRKQWGSGKEDREQ VIG in isoform 3.		VSP_030137
	Alternative sequence	44 – 104	61	Missing in isoform 2 and isoform 6.		VSP_030139

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 0B49E32FF89CD975	FASTA	278	31,448
	10 20 30 40 50 60 MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDEDDEEE EDSGAWGRES YAFDGFHPTE 70 80 90 100 110 120 EFGFSFSPRG GMRFHGNFGF DDLVRDFNSI FSEMGAWTLP SHSPELPGPE SETPGVRLRE 130 140 150 160 170 180 GQTLRDSMLK YPDSHQPRIF EGVLESHAKP ESSKPAPDWG SQGPFHRLDD TWPVSPHSRA 190 200 210 220 230 240 REDKDLDSQV SQEGLGPLLQ PQPKSYFKSI SVTKITKPDG TVEEHRTVVD SEGRRETTVT 250 260 270 HQEAHDSSRS DPDPPRSSAL DDPFSILDLL LGRWFRSR
	Isoform 2. Checksum: 822F48152513778E	FASTA	217	24,572
	10 20 30 40 50 60 MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDEDDEEE EDSELPGPES ETPGVRLREG 70 80 90 100 110 120 QTLRDSMLKY PDSHQPRIFE GVLESHAKPE SSKPAPDWGS QGPFHRLDDT WPVSPHSRAR 130 140 150 160 170 180 EDKDLDSQVS QEGLGPLLQP QPKSYFKSIS VTKITKPDGT VEEHRTVVDS EGRRETTVTH 190 200 210 QEAHDSSRSD PDPPRSSALD DPFSILDLLL GRWFRSR
	Isoform 3. Checksum: E7BB60A4895ECB21	FASTA	283	32,084
	10 20 30 40 50 60 MQQGPERRKQ WGSGKEDREQ VIGHRDPFFG GMTRDDDDDE DDEEEEDSGA WGRESYAFDG 70 80 90 100 110 120 FHPTEEFGFS FSPRGGMRFH GNFGFDDLVR DFNSIFSEMG AWTLPSHSPE LPGPESETPG 130 140 150 160 170 180 VRLREGQTLR DSMLKYPDSH QPRIFEGVLE SHAKPESSKP APDWGSQGPF HRLDDTWPVS 190 200 210 220 230 240 PHSRAREDKD LDSQVSQEGL GPLLQPQPKS YFKSISVTKI TKPDGTVEEH RTVVDSEGRR 250 260 270 280 ETTVTHQEAH DSSRSDPDPP RSSALDDPFS ILDLLLGRWF RSR
	Isoform 5. Checksum: D3163FE42C27D946	FASTA	252	28,488
	10 20 30 40 50 60 MTRDDDDDED DEEEEDSGAW GRESYAFDGF HPTEEFGFSF SPRGGMRFHG NFGFDDLVRD 70 80 90 100 110 120 FNSIFSEMGA WTLPSHSPEL PGPESETPGV RLREGQTLRD SMLKYPDSHQ PRIFEGVLES 130 140 150 160 170 180 HAKPESSKPA PDWGSQGPFH RLDDTWPVSP HSRAREDKDL DSQVSQEGLG PLLQPQPKSY 190 200 210 220 230 240 FKSISVTKIT KPDGTVEEHR TVVDSEGRRE TTVTHQEAHD SSRSDPDPPR SSALDDPFSI 250 LDLLLGRWFR SR
	Isoform 6. Checksum: F0C0BA00B2450DA2	FASTA	191	21,611
	10 20 30 40 50 60 MTRDDDDDED DEEEEDSELP GPESETPGVR LREGQTLRDS MLKYPDSHQP RIFEGVLESH 70 80 90 100 110 120 AKPESSKPAP DWGSQGPFHR LDDTWPVSPH SRAREDKDLD SQVSQEGLGP LLQPQPKSYF 130 140 150 160 170 180 KSISVTKITK PDGTVEEHRT VVDSEGRRET TVTHQEAHDS SRSDPDPPRS SALDDPFSIL 190 DLLLGRWFRS R

References

[1]	"Identification and expression analysis of alternative splice variants of the rat Hax-1 gene." Grzybowska E.A., Sarnowska E., Konopinski R., Wilczynska A., Sarnowski T.J., Siedlecki J.A. Gene 371:84-92(2006) [PubMed: 16516414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 5 AND 6). Strain: Lewis. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY291062 mRNA. Translation: AAP44974.1. AY291063 mRNA. Translation: AAP44975.1. AY291064 mRNA. Translation: AAP44976.1. AY919342 mRNA. Translation: AAX18866.1. AY919343 mRNA. Translation: AAX18867.1. DQ286293 Genomic DNA. Translation: ABB91376.1.
IPI	IPI00382352. IPI00555236. IPI00766770. IPI00882515. IPI00882561.
RefSeq	NP_853658.1.
UniGene	Rn.185269
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TSE9. 4 interactions.
Genome annotation databases
GeneID	291202.
KEGG	rno:291202.
Organism-specific databases
CTD	291202.
RGD	727960. Hs1bp1.
Phylogenomic databases
HOVERGEN	Q7TSE9.
Gene expression databases
Genevestigator	Q7TSE9.
Family and domain databases
InterPro	IPR017248. HS1--assoc_X-1. [Graphical view]
PIRSF	PIRSF037634. HS1-associating_X-1. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	632255.

Entry information

Entry name

HAX1_RAT

Accession

Primary (citable) accession number: Q7TSE9
Secondary accession number(s): Q5D1N3 , Q5D1N4, Q7TSE7, Q7TSE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2003
Last modified:	October 13, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)