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Protein

HCLS1-associated protein X-1

Gene

Hax1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.By similarity2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
HCLS1-associated protein X-1
Alternative name(s):
HS1-associating protein X-1
Short name:
HAX-1
HS1-binding protein 1
Short name:
HSP1BP-1
Gene namesi
Name:Hax1
Synonyms:Hs1bp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727960. Hax1.

Subcellular locationi

Isoform 1 :
  • Cytoplasm 1 Publication
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Also detected in the nucleus when nuclear export is inhibited, and in response to cellular stress caused by arsenite (in vitro).By similarity

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cell cortex Source: UniProtKB-SubCell
  • clathrin-coated vesicle Source: RGD
  • cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrion Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB-SubCell
  • sarcoplasmic reticulum Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003138012 – 278HCLS1-associated protein X-1Add BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126 – 127Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7TSE9.
PRIDEiQ7TSE9.

Expressioni

Tissue specificityi

Present in striated muscles (at protein level).1 Publication

Developmental stagei

Abundant in hindlimb and cardiac muscles throughout embryogenesis (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000045647.

Interactioni

Subunit structurei

Interacts with ABCB1, ABCB4 and ABCB11 (PubMed:15159385). Directly associates with HCLS1/HS1, through binding to its N-terminal region (By similarity). Interacts with CTTN (PubMed:15159385). Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with TPC2. Interacts with KCNC3. Interacts with XPO1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Abcb11O701275EBI-930005,EBI-930036
Abcb4Q082013EBI-930005,EBI-929988
Mdr1aQ6PSM02EBI-930005,EBI-930055

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ7TSE9. 4 interactors.
STRINGi10116.ENSRNOP00000067159.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 43Required for localization in mitochondriaAdd BLAST42
Regioni113 – 278Involved in HCLS1 bindingBy similarityAdd BLAST166
Regioni174 – 205Involved in CASP9 bindingBy similarityAdd BLAST32
Regioni175 – 246Involved in GNA13 bindingBy similarityAdd BLAST72
Regioni182 – 278Required for localization in sarcoplasmic reticulumAdd BLAST97
Regioni183 – 278Involved in PKD2 bindingBy similarityAdd BLAST96
Regioni202 – 244Involved in ATP2A2 bindingBy similarityAdd BLAST43
Regioni202 – 224Involved in PLN bindingBy similarityAdd BLAST23
Regioni269 – 278Required for ITGB6 bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 42Asp/Glu-rich (highly acidic)Add BLAST13

Sequence similaritiesi

Belongs to the HAX1 family.Curated

Phylogenomic databases

HOVERGENiHBG002991.
InParanoidiQ7TSE9.
KOiK16220.
PhylomeDBiQ7TSE9.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVFDLFRGF FGFPGPRSHR DPFFGGMTRD DDDDEDDEEE EDSGAWGRES
60 70 80 90 100
YAFDGFHPTE EFGFSFSPRG GMRFHGNFGF DDLVRDFNSI FSEMGAWTLP
110 120 130 140 150
SHSPELPGPE SETPGVRLRE GQTLRDSMLK YPDSHQPRIF EGVLESHAKP
160 170 180 190 200
ESSKPAPDWG SQGPFHRLDD TWPVSPHSRA REDKDLDSQV SQEGLGPLLQ
210 220 230 240 250
PQPKSYFKSI SVTKITKPDG TVEEHRTVVD SEGRRETTVT HQEAHDSSRS
260 270
DPDPPRSSAL DDPFSILDLL LGRWFRSR
Length:278
Mass (Da):31,448
Last modified:October 1, 2003 - v1
Checksum:i0B49E32FF89CD975
GO
Isoform 2 (identifier: Q7TSE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-104: Missing.

Show »
Length:217
Mass (Da):24,572
Checksum:i822F48152513778E
GO
Isoform 3 (identifier: Q7TSE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSVFDLFRGFFGFPGPRS → MQQGPERRKQWGSGKEDREQVIG

Show »
Length:283
Mass (Da):32,084
Checksum:iE7BB60A4895ECB21
GO
Isoform 5 (identifier: Q7TSE9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:252
Mass (Da):28,488
Checksum:iD3163FE42C27D946
GO
Isoform 6 (identifier: Q7TSE9-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.
     44-104: Missing.

Show »
Length:191
Mass (Da):21,611
Checksum:iF0C0BA00B2450DA2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0301381 – 26Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_0301371 – 18MSVFD…PGPRS → MQQGPERRKQWGSGKEDREQ VIG in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_03013944 – 104Missing in isoform 2 and isoform 6. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA. Translation: AAP44974.1.
AY291063 mRNA. Translation: AAP44975.1.
AY291064 mRNA. Translation: AAP44976.1.
AY919342 mRNA. Translation: AAX18866.1.
AY919343 mRNA. Translation: AAX18867.1.
DQ286293 Genomic DNA. Translation: ABB91376.1.
RefSeqiNP_853658.1. NM_181627.2. [Q7TSE9-1]
UniGeneiRn.185269.

Genome annotation databases

GeneIDi291202.
KEGGirno:291202.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY291062 mRNA. Translation: AAP44974.1.
AY291063 mRNA. Translation: AAP44975.1.
AY291064 mRNA. Translation: AAP44976.1.
AY919342 mRNA. Translation: AAX18866.1.
AY919343 mRNA. Translation: AAX18867.1.
DQ286293 Genomic DNA. Translation: ABB91376.1.
RefSeqiNP_853658.1. NM_181627.2. [Q7TSE9-1]
UniGeneiRn.185269.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TSE9. 4 interactors.
STRINGi10116.ENSRNOP00000067159.

Proteomic databases

PaxDbiQ7TSE9.
PRIDEiQ7TSE9.

Protocols and materials databases

DNASUi291202.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi291202.
KEGGirno:291202.

Organism-specific databases

CTDi10456.
RGDi727960. Hax1.

Phylogenomic databases

HOVERGENiHBG002991.
InParanoidiQ7TSE9.
KOiK16220.
PhylomeDBiQ7TSE9.

Miscellaneous databases

PROiQ7TSE9.

Gene expression databases

BgeeiENSRNOG00000045647.

Family and domain databases

InterProiIPR017248. HAX-1.
[Graphical view]
PIRSFiPIRSF037634. HS1-associating_X-1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHAX1_RAT
AccessioniPrimary (citable) accession number: Q7TSE9
Secondary accession number(s): Q5D1N3
, Q5D1N4, Q7TSE7, Q7TSE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.