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Protein

Metastasis-suppressor KiSS-1

Gene

Kiss1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.2 Publications

GO - Molecular functioni

  • G-protein coupled receptor binding Source: RGD
  • kisspeptin receptor binding Source: RGD

GO - Biological processi

  • generation of ovulation cycle rhythm Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: RGD
  • positive regulation of growth hormone secretion Source: RGD
  • positive regulation of luteinizing hormone secretion Source: RGD
  • positive regulation of MAPK cascade Source: RGD
  • positive regulation of synaptic transmission Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Metastasis-suppressor KiSS-1
Alternative name(s):
Kisspeptin-1
Cleaved into the following 2 chains:
Alternative name(s):
Metastin45-54
Gene namesi
Name:Kiss1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi727850. Kiss1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002155520 – 130Metastasis-suppressor KiSS-1Add BLAST111
PeptideiPRO_000002155668 – 119MetastinAdd BLAST52
PeptideiPRO_0000021557110 – 119Kisspeptin-1010

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 85Sequence analysis
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei119Tyrosine amideBy similarity1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ7TSB7.
PRIDEiQ7TSB7.

PTM databases

PhosphoSitePlusiQ7TSB7.

Expressioni

Tissue specificityi

Highest levels in the cecum and colon. Moderate levels present in the liver, spleen, kidney, ovary, uterus and small intestine. Low levels in the stomach, pancreas and placenta. Expressed only moderately in the placenta. Persistent expression is detected in hypothalamus throughout postnatal development, with maximum expression levels at puberty in both male and female. Hypothalamic expression is sensitive to neonatal imprinting by estrogen. Expression is higher in the hypothalamus than in the brainstem and spinal cord. In the brain, metastin-like immunoreactivity is found mainly in three groups of cells: dorsomedial hypothalamic nucleus, nucleus of the solitary tract, and caudal ventrolateral medulla.3 Publications

Developmental stagei

Expression observed in trophoblast giant cells (TGCs), the placenta-derived cell lineage aligned at the boundary between the uterus and placenta, at embryonic days 12.5 (E12.5). Expressions gradually decreased during placental maturation, and the signal is no more detectable in the cells at E18.5.1 Publication

Gene expression databases

BgeeiENSRNOG00000047481.
ExpressionAtlasiQ7TSB7. differential.

Interactioni

GO - Molecular functioni

  • G-protein coupled receptor binding Source: RGD
  • kisspeptin receptor binding Source: RGD

Protein-protein interaction databases

BioGridi252772. 1 interactor.
STRINGi10116.ENSRNOP00000032239.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 119Essential for receptor binding and receptor activation10

Sequence similaritiesi

Belongs to the KISS1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYVQ. Eukaryota.
ENOG41118MU. LUCA.
GeneTreeiENSGT00390000008827.
HOGENOMiHOG000013123.
HOVERGENiHBG052261.
InParanoidiQ7TSB7.
OMAiRQIPAPQ.
OrthoDBiEOG091G1591.

Family and domain databases

InterProiIPR020207. Metastasis-suppressor_KiSS-1.
[Graphical view]
PfamiPF15152. Kisspeptin. 1 hit.
[Graphical view]
ProDomiPD088121. Metastasis-suppressor_KiSS-1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TSB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISLASWQLL LLLCVASFGE PLAKMAPVVN PEPTGQQSGP QELVNAWQKG
60 70 80 90 100
PRYAESKPGA AGLRARRTSP CPPVENPTGH QRPPCATRSR LIPAPRGSVL
110 120 130
VQREKDMSAY NWNSFGLRYG RRQVARAARG
Length:130
Mass (Da):14,188
Last modified:October 1, 2003 - v1
Checksum:i9ADEADA018BF5868
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY196983 mRNA. Translation: AAP22375.1.
RefSeqiNP_859043.1. NM_181692.1.
XP_008767665.1. XM_008769443.2.
XP_017454186.1. XM_017598697.1.
UniGeneiRn.66008.

Genome annotation databases

EnsembliENSRNOT00000076506; ENSRNOP00000068332; ENSRNOG00000047481.
ENSRNOT00000077054; ENSRNOP00000068288; ENSRNOG00000047481.
GeneIDi289023.
KEGGirno:289023.
UCSCiRGD:727850. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY196983 mRNA. Translation: AAP22375.1.
RefSeqiNP_859043.1. NM_181692.1.
XP_008767665.1. XM_008769443.2.
XP_017454186.1. XM_017598697.1.
UniGeneiRn.66008.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252772. 1 interactor.
STRINGi10116.ENSRNOP00000032239.

PTM databases

PhosphoSitePlusiQ7TSB7.

Proteomic databases

PaxDbiQ7TSB7.
PRIDEiQ7TSB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000076506; ENSRNOP00000068332; ENSRNOG00000047481.
ENSRNOT00000077054; ENSRNOP00000068288; ENSRNOG00000047481.
GeneIDi289023.
KEGGirno:289023.
UCSCiRGD:727850. rat.

Organism-specific databases

CTDi3814.
RGDi727850. Kiss1.

Phylogenomic databases

eggNOGiENOG410IYVQ. Eukaryota.
ENOG41118MU. LUCA.
GeneTreeiENSGT00390000008827.
HOGENOMiHOG000013123.
HOVERGENiHBG052261.
InParanoidiQ7TSB7.
OMAiRQIPAPQ.
OrthoDBiEOG091G1591.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiQ7TSB7.

Gene expression databases

BgeeiENSRNOG00000047481.
ExpressionAtlasiQ7TSB7. differential.

Family and domain databases

InterProiIPR020207. Metastasis-suppressor_KiSS-1.
[Graphical view]
PfamiPF15152. Kisspeptin. 1 hit.
[Graphical view]
ProDomiPD088121. Metastasis-suppressor_KiSS-1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiKISS1_RAT
AccessioniPrimary (citable) accession number: Q7TSB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.