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Q7TSA3

- BTLA_MOUSE

UniProt

Q7TSA3 - BTLA_MOUSE

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Protein
B- and T-lymphocyte attenuator
Gene
Btla
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.

GO - Molecular functioni

  1. protein binding Source: MGI
  2. receptor activity Source: MGI

GO - Biological processi

  1. cell surface receptor signaling pathway Source: MGI
  2. immune response-regulating cell surface receptor signaling pathway Source: MGI
  3. immune system process Source: UniProtKB-KW
  4. negative regulation of B cell proliferation Source: MGI
  5. negative regulation of T cell proliferation Source: MGI
  6. negative regulation of alpha-beta T cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_225477. Costimulation by the CD28 family.

Names & Taxonomyi

Protein namesi
Recommended name:
B- and T-lymphocyte attenuator
Alternative name(s):
B- and T-lymphocyte-associated protein
CD_antigen: CD272
Gene namesi
Name:Btla
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:2658978. Btla.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 183154Extracellular Reviewed prediction
Add
BLAST
Transmembranei184 – 20421Helical; Reviewed prediction
Add
BLAST
Topological domaini205 – 306102Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice exhibit no developmental defects in T- or B-cells in thymus or bone marrow, but increased antibody responses and sensitivity to antigen-induced 'experimental autoimmune encephalomyelitis'. T-cells lacking Btla show increased proliferation with a heightened response to anti-CD3 and a slightly greater response to stimulation with anti-IgM.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 441L → H: Loss of interaction with TNFRSF14. 1 Publication
Mutagenesisi48 – 481R → D: Loss of interaction with TNFRSF14. 1 Publication
Mutagenesisi65 – 651P → A: Loss of interaction with TNFRSF14. 1 Publication
Mutagenesisi136 – 1361H → D: Loss of interaction with TNFRSF14. 1 Publication
Mutagenesisi245 – 2451Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-274 and/or F-299. 2 Publications
Mutagenesisi274 – 2741Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-245 and/or F-299. 2 Publications
Mutagenesisi299 – 2991Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-245 and/or F-274. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 306277B- and T-lymphocyte attenuator
PRO_0000014524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 691 Publication
Disulfide bondi64 ↔ 1241 Publication
Glycosylationi74 – 741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi78 ↔ 851 Publication
Glycosylationi81 – 811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi101 – 1011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi119 – 1191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi148 – 1481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi165 – 1651N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ7TSA3.

PTM databases

PhosphoSiteiQ7TSA3.

Expressioni

Tissue specificityi

Expressed in splenic T- and B-cells as well as lymph node tissues but very weakly in somatic tissues. Also expressed in macrophages, NK cells and dendritic cells. A polymorphic tissue distribution between several strains is seen.2 Publications

Gene expression databases

ArrayExpressiQ7TSA3.
BgeeiQ7TSA3.
CleanExiMM_BTLA.
GenevestigatoriQ7TSA3.

Interactioni

Subunit structurei

Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Interacts with TNFRSF14/HVEM.4 Publications

Protein-protein interaction databases

BioGridi228954. 1 interaction.
STRINGi10090.ENSMUSP00000067877.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi50 – 556
Beta strandi60 – 678
Beta strandi69 – 713
Beta strandi75 – 806
Beta strandi82 – 876
Beta strandi94 – 996
Beta strandi101 – 1044
Beta strandi106 – 1116
Helixi116 – 1183
Beta strandi120 – 1289
Beta strandi131 – 1344
Beta strandi138 – 1436

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XAUX-ray1.80A30-150[»]
ProteinModelPortaliQ7TSA3.
SMRiQ7TSA3. Positions 40-143.

Miscellaneous databases

EvolutionaryTraceiQ7TSA3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 139103Ig-like V-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG42530.
GeneTreeiENSGT00390000017390.
HOGENOMiHOG000168895.
HOVERGENiHBG080937.
InParanoidiQ7TSA3.
KOiK06707.
OrthoDBiEOG7F513T.
PhylomeDBiQ7TSA3.
TreeFamiTF337694.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7TSA3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKTVPAMLGT PRLFREFFIL HLGLWSILCE KATKRNDEEC PVQLTITRNS    50
KQSARTGELF KIQCPVKYCV HRPNVTWCKH NGTICVPLEV SPQLYTSWEE 100
NQSVPVFVLH FKPIHLSDNG SYSCSTNFNS QVINSHSVTI HVRERTQNSS 150
EHPLITVSDI PDATNASGPS TMEERPGRTW LLYTLLPLGA LLLLLACVCL 200
LCFLKRIQGK EKKPSDLAGR DTNLVDIPAS SRTNHQALPS GTGIYDNDPW 250
SSMQDESELT ISLQSERNNQ GIVYASLNHC VIGRNPRQEN NMQEAPTEYA 300
SICVRS 306
Length:306
Mass (Da):34,337
Last modified:July 19, 2005 - v2
Checksum:iA5B4584BAC882B93
GO
Isoform 2 (identifier: Q7TSA3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-142: Missing.

Show »
Length:200
Mass (Da):22,326
Checksum:i94A167B779171DF0
GO
Isoform 3 (identifier: Q7TSA3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-157: TV → I

Show »
Length:305
Mass (Da):34,250
Checksum:i14C51F126F2F519A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti41 – 411P → E in strain: 129/SvEv; requires 2 nucleotide substitutions. 1 Publication
Natural varianti45 – 473TIT → NIK in strain: 129/SvEv.
Natural varianti52 – 521Q → H in strain: 129/SvEv. 1 Publication
Natural varianti55 – 551R → W in strain: 129/SvEv. 1 Publication
Natural varianti63 – 631Q → E in strain: 129/SvEv. 1 Publication
Natural varianti85 – 851C → W in strain: 129/SvEv. 1 Publication
Natural varianti91 – 911S → G in strain: 129/SvEv. 1 Publication
Natural varianti102 – 1021Q → R in strain: 129/SvEv. 1 Publication
Natural varianti143 – 1431R → T in strain: C57BL/6J. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei37 – 142106Missing in isoform 2.
VSP_014836Add
BLAST
Alternative sequencei156 – 1572TV → I in isoform 3.
VSP_014837

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY293285 mRNA. Translation: AAP44002.1.
AK041334 mRNA. Translation: BAC30910.1.
CCDSiCCDS28195.1. [Q7TSA3-1]
CCDS28196.1. [Q7TSA3-3]
RefSeqiNP_001032808.2. NM_001037719.2.
NP_808252.1. NM_177584.3.
UniGeneiMm.38199.

Genome annotation databases

EnsembliENSMUST00000102802; ENSMUSP00000099866; ENSMUSG00000052013.
GeneIDi208154.
KEGGimmu:208154.
UCSCiuc007zik.1. mouse. [Q7TSA3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY293285 mRNA. Translation: AAP44002.1 .
AK041334 mRNA. Translation: BAC30910.1 .
CCDSi CCDS28195.1. [Q7TSA3-1 ]
CCDS28196.1. [Q7TSA3-3 ]
RefSeqi NP_001032808.2. NM_001037719.2.
NP_808252.1. NM_177584.3.
UniGenei Mm.38199.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1XAU X-ray 1.80 A 30-150 [» ]
ProteinModelPortali Q7TSA3.
SMRi Q7TSA3. Positions 40-143.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 228954. 1 interaction.
STRINGi 10090.ENSMUSP00000067877.

PTM databases

PhosphoSitei Q7TSA3.

Proteomic databases

PRIDEi Q7TSA3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102802 ; ENSMUSP00000099866 ; ENSMUSG00000052013 .
GeneIDi 208154.
KEGGi mmu:208154.
UCSCi uc007zik.1. mouse. [Q7TSA3-3 ]

Organism-specific databases

CTDi 151888.
MGIi MGI:2658978. Btla.

Phylogenomic databases

eggNOGi NOG42530.
GeneTreei ENSGT00390000017390.
HOGENOMi HOG000168895.
HOVERGENi HBG080937.
InParanoidi Q7TSA3.
KOi K06707.
OrthoDBi EOG7F513T.
PhylomeDBi Q7TSA3.
TreeFami TF337694.

Enzyme and pathway databases

Reactomei REACT_225477. Costimulation by the CD28 family.

Miscellaneous databases

EvolutionaryTracei Q7TSA3.
NextBioi 372176.
PROi Q7TSA3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q7TSA3.
Bgeei Q7TSA3.
CleanExi MM_BTLA.
Genevestigatori Q7TSA3.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF TYR-245; TYR-274 AND TYR-299, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH PTPN6 AND PTPN11, DISRUPTION PHENOTYPE, VARIANTS GLU-41; 45-ASN--LYS-47; HIS-52; TRP-55; GLU-63; TRP-85; GLY-91 AND ARG-102.
    Strain: 129/SvEv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT THR-143.
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "Characterization of phosphotyrosine binding motifs in the cytoplasmic domain of B and T lymphocyte attenuator required for association with protein tyrosine phosphatases SHP-1 and SHP-2."
    Gavrieli M., Watanabe N., Loftin S.K., Murphy T.L., Murphy K.M.
    Biochem. Biophys. Res. Commun. 312:1236-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TYR-245; TYR-274 AND TYR-299, INTERACTION WITH PTPN6 AND PTPN11.
  4. "B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator."
    Sedy J.R., Gavrieli M., Potter K.G., Hurchla M.A., Lindsley R.C., Hildner K., Scheu S., Pfeffer K., Ware C.F., Murphy T.L., Murphy K.M.
    Nat. Immunol. 6:90-98(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TNFRSF14, PHOSPHORYLATION.
  5. "B and T lymphocyte attenuator exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T cells."
    Hurchla M.A., Sedy J.R., Gavrieli M., Drake C.G., Murphy T.L., Murphy K.M.
    J. Immunol. 174:3377-3385(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, POLYMORPHISM.
  6. "Structural determinants of herpesvirus entry mediator recognition by murine B and T lymphocyte attenuator."
    Nelson C.A., Fremont M.D., Sedy J.R., Norris P.S., Ware C.F., Murphy K.M., Fremont D.H.
    J. Immunol. 180:940-947(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 30-150, MUTAGENESIS OF LEU-44; ARG-48; PRO-65 AND HIS-136, INTERACTION WITH TNFRSF14/HVEM, DISULFIDE BONDS.

Entry informationi

Entry nameiBTLA_MOUSE
AccessioniPrimary (citable) accession number: Q7TSA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 3, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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