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Protein

B- and T-lymphocyte attenuator

Gene

Btla

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.

GO - Molecular functioni

  • receptor activity Source: MGI

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: MGI
  • immune response-regulating cell surface receptor signaling pathway Source: MGI
  • negative regulation of alpha-beta T cell proliferation Source: MGI
  • negative regulation of B cell proliferation Source: MGI
  • negative regulation of T cell proliferation Source: MGI
  • T cell costimulation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Names & Taxonomyi

Protein namesi
Recommended name:
B- and T-lymphocyte attenuator
Alternative name(s):
B- and T-lymphocyte-associated protein
CD_antigen: CD272
Gene namesi
Name:Btla
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2658978. Btla.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 183ExtracellularSequence analysisAdd BLAST154
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Topological domaini205 – 306CytoplasmicSequence analysisAdd BLAST102

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice exhibit no developmental defects in T- or B-cells in thymus or bone marrow, but increased antibody responses and sensitivity to antigen-induced 'experimental autoimmune encephalomyelitis'. T-cells lacking Btla show increased proliferation with a heightened response to anti-CD3 and a slightly greater response to stimulation with anti-IgM.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44L → H: Loss of interaction with TNFRSF14. 1 Publication1
Mutagenesisi48R → D: Loss of interaction with TNFRSF14. 1 Publication1
Mutagenesisi65P → A: Loss of interaction with TNFRSF14. 1 Publication1
Mutagenesisi136H → D: Loss of interaction with TNFRSF14. 1 Publication1
Mutagenesisi245Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-274 and/or F-299. 2 Publications1
Mutagenesisi274Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-245 and/or F-299. 2 Publications1
Mutagenesisi299Y → F: No change of phosphorylation implicated in interaction with PTPN6 and PTPN11. Severe reduction of phosphorylation; when associated with F-245 and/or F-274. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001452430 – 306B- and T-lymphocyte attenuatorAdd BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 69PROSITE-ProRule annotation1 Publication
Disulfide bondi64 ↔ 124PROSITE-ProRule annotation1 Publication
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi78 ↔ 85PROSITE-ProRule annotation1 Publication
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi148N-linked (GlcNAc...)Sequence analysis1
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.1 Publication
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ7TSA3.
PRIDEiQ7TSA3.

PTM databases

iPTMnetiQ7TSA3.
PhosphoSitePlusiQ7TSA3.

Expressioni

Tissue specificityi

Expressed in splenic T- and B-cells as well as lymph node tissues but very weakly in somatic tissues. Also expressed in macrophages, NK cells and dendritic cells. A polymorphic tissue distribution between several strains is seen.2 Publications

Gene expression databases

BgeeiENSMUSG00000052013.
CleanExiMM_BTLA.

Interactioni

Subunit structurei

Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Interacts with TNFRSF14/HVEM.4 Publications

Protein-protein interaction databases

BioGridi228954. 1 interactor.
STRINGi10090.ENSMUSP00000067877.

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 55Combined sources6
Beta strandi60 – 67Combined sources8
Beta strandi69 – 71Combined sources3
Beta strandi75 – 80Combined sources6
Beta strandi82 – 87Combined sources6
Beta strandi94 – 99Combined sources6
Beta strandi101 – 104Combined sources4
Beta strandi106 – 111Combined sources6
Helixi116 – 118Combined sources3
Beta strandi120 – 128Combined sources9
Beta strandi131 – 134Combined sources4
Beta strandi138 – 143Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XAUX-ray1.80A30-150[»]
ProteinModelPortaliQ7TSA3.
SMRiQ7TSA3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7TSA3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 139Ig-like V-typeAdd BLAST103

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYZD. Eukaryota.
ENOG410ZATJ. LUCA.
HOGENOMiHOG000168895.
HOVERGENiHBG080937.
InParanoidiQ7TSA3.
KOiK06707.
PhylomeDBiQ7TSA3.
TreeFamiTF337694.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TSA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTVPAMLGT PRLFREFFIL HLGLWSILCE KATKRNDEEC PVQLTITRNS
60 70 80 90 100
KQSARTGELF KIQCPVKYCV HRPNVTWCKH NGTICVPLEV SPQLYTSWEE
110 120 130 140 150
NQSVPVFVLH FKPIHLSDNG SYSCSTNFNS QVINSHSVTI HVRERTQNSS
160 170 180 190 200
EHPLITVSDI PDATNASGPS TMEERPGRTW LLYTLLPLGA LLLLLACVCL
210 220 230 240 250
LCFLKRIQGK EKKPSDLAGR DTNLVDIPAS SRTNHQALPS GTGIYDNDPW
260 270 280 290 300
SSMQDESELT ISLQSERNNQ GIVYASLNHC VIGRNPRQEN NMQEAPTEYA

SICVRS
Length:306
Mass (Da):34,337
Last modified:July 19, 2005 - v2
Checksum:iA5B4584BAC882B93
GO
Isoform 2 (identifier: Q7TSA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     37-142: Missing.

Show »
Length:200
Mass (Da):22,326
Checksum:i94A167B779171DF0
GO
Isoform 3 (identifier: Q7TSA3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-157: TV → I

Show »
Length:305
Mass (Da):34,250
Checksum:i14C51F126F2F519A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti41P → E in strain: 129/SvEv; requires 2 nucleotide substitutions. 1 Publication1
Natural varianti45 – 47TIT → NIK in strain: 129/SvEv. 3
Natural varianti52Q → H in strain: 129/SvEv. 1 Publication1
Natural varianti55R → W in strain: 129/SvEv. 1 Publication1
Natural varianti63Q → E in strain: 129/SvEv. 1 Publication1
Natural varianti85C → W in strain: 129/SvEv. 1 Publication1
Natural varianti91S → G in strain: 129/SvEv. 1 Publication1
Natural varianti102Q → R in strain: 129/SvEv. 1 Publication1
Natural varianti143R → T in strain: C57BL/6J. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01483637 – 142Missing in isoform 2. 1 PublicationAdd BLAST106
Alternative sequenceiVSP_014837156 – 157TV → I in isoform 3. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293285 mRNA. Translation: AAP44002.1.
AK041334 mRNA. Translation: BAC30910.1.
CCDSiCCDS28195.1. [Q7TSA3-1]
CCDS28196.1. [Q7TSA3-3]
RefSeqiNP_001032808.2. NM_001037719.2.
NP_808252.1. NM_177584.3.
UniGeneiMm.38199.

Genome annotation databases

GeneIDi208154.
KEGGimmu:208154.
UCSCiuc007zik.1. mouse. [Q7TSA3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293285 mRNA. Translation: AAP44002.1.
AK041334 mRNA. Translation: BAC30910.1.
CCDSiCCDS28195.1. [Q7TSA3-1]
CCDS28196.1. [Q7TSA3-3]
RefSeqiNP_001032808.2. NM_001037719.2.
NP_808252.1. NM_177584.3.
UniGeneiMm.38199.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XAUX-ray1.80A30-150[»]
ProteinModelPortaliQ7TSA3.
SMRiQ7TSA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228954. 1 interactor.
STRINGi10090.ENSMUSP00000067877.

PTM databases

iPTMnetiQ7TSA3.
PhosphoSitePlusiQ7TSA3.

Proteomic databases

PaxDbiQ7TSA3.
PRIDEiQ7TSA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi208154.
KEGGimmu:208154.
UCSCiuc007zik.1. mouse. [Q7TSA3-3]

Organism-specific databases

CTDi151888.
MGIiMGI:2658978. Btla.

Phylogenomic databases

eggNOGiENOG410IYZD. Eukaryota.
ENOG410ZATJ. LUCA.
HOGENOMiHOG000168895.
HOVERGENiHBG080937.
InParanoidiQ7TSA3.
KOiK06707.
PhylomeDBiQ7TSA3.
TreeFamiTF337694.

Enzyme and pathway databases

ReactomeiR-MMU-388841. Costimulation by the CD28 family.

Miscellaneous databases

EvolutionaryTraceiQ7TSA3.
PROiQ7TSA3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052013.
CleanExiMM_BTLA.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTLA_MOUSE
AccessioniPrimary (citable) accession number: Q7TSA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.