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Protein

Hairy and enhancer of split-related protein HELT

Gene

Helt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. Required for the development of GABAergic neurons.4 Publications

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein homodimerization activity Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • central nervous system development Source: MGI
  • GABAergic neuron differentiation in basal ganglia Source: MGI
  • gene expression Source: MGI
  • multicellular organism aging Source: MGI
  • multicellular organism growth Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuron differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • post-embryonic development Source: MGI
  • suckling behavior Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hairy and enhancer of split-related protein HELT
Alternative name(s):
HES/HEY-like transcription factor
Protein Hes-like
Protein megane
Gene namesi
Name:Helt
Synonyms:Hesl, Mgn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:3040955. Helt.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death between 2 and 5 weeks of age. The mesencephalic GABAergic progenitors in these animals fail to develop into neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Hairy and enhancer of split-related protein HELTPRO_0000349379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ7TS99.
PRIDEiQ7TS99.

PTM databases

PhosphoSiteiQ7TS99.

Expressioni

Tissue specificityi

Expressed in heart and testis.

Developmental stagei

Expressed in the progenitor domains for mesencephalic GABAergic neurons.4 Publications

Gene expression databases

BgeeiQ7TS99.

Interactioni

Subunit structurei

Self-associates. Interacts with HES5 and HEY2.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi231505. 3 interactions.
STRINGi10090.ENSMUSP00000054823.

Structurei

3D structure databases

ProteinModelPortaliQ7TS99.
SMRiQ7TS99. Positions 14-68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 6556bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini86 – 12136OrangePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HEY family.Curated
Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00700000104130.
HOGENOMiHOG000082510.
HOVERGENiHBG054492.
InParanoidiQ7TS99.
OMAiPVSHKVI.
OrthoDBiEOG72G183.
PhylomeDBiQ7TS99.
TreeFamiTF323617.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TS99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRLKERKR TPVSHKVIEK RRRDRINRCL NELGKTVPMA LAKQSSGKLE
60 70 80 90 100
KAEILEMTVQ YLRALHSADF PRGREKELLA EFANYFHYGY HECMKNLVHY
110 120 130 140 150
LTTVERMETK DTKYARILAF LQSKARLGAE PTFPPLSLPE PDFSYQLHAA
160 170 180 190 200
SPEFPGHSPG EATMFPQGAT PGSFPWPPGA ARSPALPYLS SATVPLPSPA
210 220 230 240
QQHSPFLAPM QGLDRHYLNL IGHGHPNGLN LHTPQHPPVL
Length:240
Mass (Da):26,973
Last modified:October 1, 2003 - v1
Checksum:i50B3821703D5294F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120968 mRNA. Translation: BAD11127.1.
AB098077 mRNA. Translation: BAC77659.1.
DQ294234 mRNA. Translation: ABB96784.1.
BC103599 mRNA. Translation: AAI03600.1.
BC103600 mRNA. Translation: AAI03601.1.
BC103601 mRNA. Translation: AAI03602.1.
CCDSiCCDS22290.1.
RefSeqiNP_776150.2. NM_173789.4.
UniGeneiMm.145631.

Genome annotation databases

EnsembliENSMUST00000058636; ENSMUSP00000054823; ENSMUSG00000047171.
GeneIDi234219.
KEGGimmu:234219.
UCSCiuc009lqb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120968 mRNA. Translation: BAD11127.1.
AB098077 mRNA. Translation: BAC77659.1.
DQ294234 mRNA. Translation: ABB96784.1.
BC103599 mRNA. Translation: AAI03600.1.
BC103600 mRNA. Translation: AAI03601.1.
BC103601 mRNA. Translation: AAI03602.1.
CCDSiCCDS22290.1.
RefSeqiNP_776150.2. NM_173789.4.
UniGeneiMm.145631.

3D structure databases

ProteinModelPortaliQ7TS99.
SMRiQ7TS99. Positions 14-68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231505. 3 interactions.
STRINGi10090.ENSMUSP00000054823.

PTM databases

PhosphoSiteiQ7TS99.

Proteomic databases

PaxDbiQ7TS99.
PRIDEiQ7TS99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058636; ENSMUSP00000054823; ENSMUSG00000047171.
GeneIDi234219.
KEGGimmu:234219.
UCSCiuc009lqb.1. mouse.

Organism-specific databases

CTDi391723.
MGIiMGI:3040955. Helt.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
GeneTreeiENSGT00700000104130.
HOGENOMiHOG000082510.
HOVERGENiHBG054492.
InParanoidiQ7TS99.
OMAiPVSHKVI.
OrthoDBiEOG72G183.
PhylomeDBiQ7TS99.
TreeFamiTF323617.

Miscellaneous databases

NextBioi382069.
PROiQ7TS99.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TS99.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Helt, a novel basic-helix-loop-helix transcriptional repressor expressed in the developing central nervous system."
    Nakatani T., Mizuhara E., Minaki Y., Sakamoto Y., Ono Y.
    J. Biol. Chem. 279:16356-16367(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SELF-ASSOCIATION, INTERACTION WITH HES5 AND HEY2, SUBCELLULAR LOCATION, DNA-BINDING.
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "Identification of a novel basic helix-loop-helix gene, Heslike, and its role in GABAergic neurogenesis."
    Miyoshi G., Bessho Y., Yamada S., Kageyama R.
    J. Neurosci. 24:3672-3682(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
  3. "Molecular characterization, structure and developmental expression of Megane bHLH factor."
    Guimera J., Vogt Weisenhorn D., Echevarria D., Martinez S., Wurst W.
    Gene 377:65-76(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: 129/SvJ.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Megane/Heslike is required for normal GABAergic differentiation in the mouse superior colliculus."
    Guimera J., Weisenhorn D.V., Wurst W.
    Development 133:3847-3857(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. "Helt determines GABAergic over glutamatergic neuronal fate by repressing Ngn genes in the developing mesencephalon."
    Nakatani T., Minaki Y., Kumai M., Ono Y.
    Development 134:2783-2793(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiHELT_MOUSE
AccessioniPrimary (citable) accession number: Q7TS99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.