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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Gene

Wfikkn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity (By similarity). Inhibits the biological activity of mature myostatin, but not activin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135 – 136Reactive bondPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

  • muscle fiber development Source: MGI
  • negative regulation of DNA binding Source: MGI
  • negative regulation of protein binding Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • palate development Source: MGI
  • skeletal system development Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI

Keywordsi

Molecular functionMetalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.954

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Alternative name(s):
Growth and differentiation factor-associated serum protein 1
Short name:
GASP-1
Short name:
mGASP-1
Gene namesi
Name:Wfikkn2
Synonyms:Gasp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2669209 Wfikkn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000030782130 – 571WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2Add BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 74By similarity
Disulfide bondi57 ↔ 78By similarity
Disulfide bondi61 ↔ 73By similarity
Disulfide bondi67 ↔ 83By similarity
Disulfide bondi129 ↔ 159By similarity
Disulfide bondi133 ↔ 152By similarity
Disulfide bondi141 ↔ 170By similarity
Disulfide bondi226 ↔ 282By similarity
Glycosylationi314N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi323 ↔ 373By similarity
Disulfide bondi332 ↔ 356By similarity
Disulfide bondi348 ↔ 369By similarity
Disulfide bondi381 ↔ 431By similarity
Disulfide bondi390 ↔ 414By similarity
Disulfide bondi406 ↔ 427By similarity
Disulfide bondi440 ↔ 510By similarity
Disulfide bondi443 ↔ 512By similarity
Disulfide bondi454 ↔ 561By similarity
Glycosylationi514N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TQN3
PRIDEiQ7TQN3

PTM databases

PhosphoSitePlusiQ7TQN3

Expressioni

Tissue specificityi

Widely expressed, with high expression in skeletal muscle and heart. Also expressed in brain, lung and testis. Weakly expressed in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000044177
GenevisibleiQ7TQN3 MM

Interactioni

Subunit structurei

Interacts with both mature and propeptide myostatin/MSTN.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2349851 interactor.
STRINGi10090.ENSMUSP00000053238

Structurei

3D structure databases

ProteinModelPortaliQ7TQN3
SMRiQ7TQN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 87WAPPROSITE-ProRule annotationAdd BLAST54
Domaini121 – 172Kazal-likePROSITE-ProRule annotationAdd BLAST52
Domaini205 – 298Ig-like C2-typeAdd BLAST94
Domaini323 – 373BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini381 – 431BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51
Domaini440 – 561NTRPROSITE-ProRule annotationAdd BLAST122

Sequence similaritiesi

Belongs to the WFIKKN family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410KDTI Eukaryota
ENOG410XRTD LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000154855
HOVERGENiHBG108676
InParanoidiQ7TQN3
OMAiCPNVCGT
OrthoDBiEOG091G09P2
PhylomeDBiQ7TQN3
TreeFamiTF315349

Family and domain databases

CDDicd00109 KU, 2 hits
Gene3Di2.60.40.101 hit
4.10.410.102 hits
4.10.75.101 hit
InterProiView protein in InterPro
IPR036645 Elafin-like_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR001134 Netrin_domain
IPR018933 Netrin_module_non-TIMP
IPR020901 Prtase_inh_Kunz-CS
IPR008993 TIMP-like_OB-fold
IPR008197 WAP_dom
IPR033639 WFIKKN2
PANTHERiPTHR10083:SF87 PTHR10083:SF87, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF00014 Kunitz_BPTI, 2 hits
PF01759 NTR, 1 hit
PF00095 WAP, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00131 KU, 2 hits
SM00217 WAP, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF48726 SSF48726, 1 hit
SSF50242 SSF50242, 1 hit
SSF57256 SSF57256, 1 hit
SSF57362 SSF57362, 2 hits
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 2 hits
PS50835 IG_LIKE, 1 hit
PS51465 KAZAL_2, 1 hit
PS50189 NTR, 1 hit
PS51390 WAP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TQN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAPGYHRFW FHWGLLLLLL LEAPLRGLAL PPIRYSHAGI CPNDMNPNLW
60 70 80 90 100
VDAQSTCKRE CETDQECETY EKCCPNVCGT KSCVAARYMD VKGKKGPVGM
110 120 130 140 150
PKEATCDHFM CLQQGSECDI WDGQPVCKCK DRCEKEPSFT CASDGLTYYN
160 170 180 190 200
RCFMDAEACS KGITLSVVTC RYHFTWPNTS PPPPETTVHP TTASPETLGL
210 220 230 240 250
DMAAPALLNH PVHQSVTVGE TVSFLCDVVG RPRPELTWEK QLEDRENVVM
260 270 280 290 300
RPNHVRGNVV VTNIAQLVIY NVQPQDAGIY TCTARNVAGV LRADFPLSVV
310 320 330 340 350
RGGQARATSE SSLNGTAFPA TECLKPPDSE DCGEEQTRWH FDAQANNCLT
360 370 380 390 400
FTFGHCHHNL NHFETYEACM LACMSGPLAI CSLPALQGPC KAYVPRWAYN
410 420 430 440 450
SQTGLCQSFV YGGCEGNGNN FESREACEES CPFPRGNQHC RACKPRQKLV
460 470 480 490 500
TSFCRSDFVI LGRVSELTEE QDSGRALVTV DEVLKDEKMG LKFLGREPLE
510 520 530 540 550
VTLLHVDWTC PCPNVTVGET PLIIMGEVDG GMAMLRPDSF VGASSTRRVR
560 570
KLREVMYKKT CDVLKDFLGL Q
Length:571
Mass (Da):63,333
Last modified:October 23, 2007 - v2
Checksum:i3A75A449F53703AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti380I → T in AAP72503 (PubMed:12595574).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY308804 mRNA Translation: AAP72503.1
AL645846 Genomic DNA No translation available.
CCDSiCCDS25251.1
RefSeqiNP_861540.2, NM_181819.2
XP_006533561.1, XM_006533498.3
XP_006533562.1, XM_006533499.3
XP_006533563.1, XM_006533500.3
XP_006533564.1, XM_006533501.3
XP_006533565.1, XM_006533502.2
XP_011247365.1, XM_011249063.2
UniGeneiMm.342216

Genome annotation databases

EnsembliENSMUST00000061469; ENSMUSP00000053238; ENSMUSG00000044177
GeneIDi278507
KEGGimmu:278507
UCSCiuc007kyg.2 mouse

Similar proteinsi

Entry informationi

Entry nameiWFKN2_MOUSE
AccessioniPrimary (citable) accession number: Q7TQN3
Secondary accession number(s): Q5SUS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: February 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome