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Protein

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Gene

Wfikkn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity (By similarity). Inhibits the biological activity of mature myostatin, but not activin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135 – 136Reactive bondPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

  • muscle fiber development Source: MGI
  • negative regulation of DNA binding Source: MGI
  • negative regulation of protein binding Source: MGI
  • palate development Source: MGI
  • skeletal system development Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.954.

Names & Taxonomyi

Protein namesi
Recommended name:
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Alternative name(s):
Growth and differentiation factor-associated serum protein 1
Short name:
GASP-1
Short name:
mGASP-1
Gene namesi
Name:Wfikkn2
Synonyms:Gasp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2669209. Wfikkn2.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000030782130 – 571WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2Add BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 74By similarity
Disulfide bondi57 ↔ 78By similarity
Disulfide bondi61 ↔ 73By similarity
Disulfide bondi67 ↔ 83By similarity
Disulfide bondi129 ↔ 159By similarity
Disulfide bondi133 ↔ 152By similarity
Disulfide bondi141 ↔ 170By similarity
Disulfide bondi226 ↔ 282By similarity
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi323 ↔ 373By similarity
Disulfide bondi332 ↔ 356By similarity
Disulfide bondi348 ↔ 369By similarity
Disulfide bondi381 ↔ 431By similarity
Disulfide bondi390 ↔ 414By similarity
Disulfide bondi406 ↔ 427By similarity
Disulfide bondi440 ↔ 510By similarity
Disulfide bondi443 ↔ 512By similarity
Disulfide bondi454 ↔ 561By similarity
Glycosylationi514N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TQN3.
PRIDEiQ7TQN3.

PTM databases

PhosphoSitePlusiQ7TQN3.

Expressioni

Tissue specificityi

Widely expressed, with high expression in skeletal muscle and heart. Also expressed in brain, lung and testis. Weakly expressed in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000044177.
GenevisibleiQ7TQN3. MM.

Interactioni

Subunit structurei

Interacts with both mature and propeptide myostatin/MSTN.1 Publication

Protein-protein interaction databases

BioGridi234985. 1 interactor.
STRINGi10090.ENSMUSP00000053238.

Structurei

3D structure databases

ProteinModelPortaliQ7TQN3.
SMRiQ7TQN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 87WAPPROSITE-ProRule annotationAdd BLAST54
Domaini121 – 172Kazal-likePROSITE-ProRule annotationAdd BLAST52
Domaini205 – 298Ig-like C2-typeAdd BLAST94
Domaini323 – 373BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini381 – 431BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51
Domaini440 – 561NTRPROSITE-ProRule annotationAdd BLAST122

Sequence similaritiesi

Belongs to the WFIKKN family.Curated
Contains 2 BPTI/Kunitz inhibitor domains.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation
Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410KDTI. Eukaryota.
ENOG410XRTD. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000154855.
HOVERGENiHBG108676.
InParanoidiQ7TQN3.
OMAiCPNVCGT.
OrthoDBiEOG091G09P2.
PhylomeDBiQ7TQN3.
TreeFamiTF315349.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR002223. Kunitz_BPTI.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP_dom.
IPR033639. WFIKKN2.
[Graphical view]
PANTHERiPTHR10083:SF87. PTHR10083:SF87. 1 hit.
PfamiPF07679. I-set. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF01759. NTR. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TQN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAPGYHRFW FHWGLLLLLL LEAPLRGLAL PPIRYSHAGI CPNDMNPNLW
60 70 80 90 100
VDAQSTCKRE CETDQECETY EKCCPNVCGT KSCVAARYMD VKGKKGPVGM
110 120 130 140 150
PKEATCDHFM CLQQGSECDI WDGQPVCKCK DRCEKEPSFT CASDGLTYYN
160 170 180 190 200
RCFMDAEACS KGITLSVVTC RYHFTWPNTS PPPPETTVHP TTASPETLGL
210 220 230 240 250
DMAAPALLNH PVHQSVTVGE TVSFLCDVVG RPRPELTWEK QLEDRENVVM
260 270 280 290 300
RPNHVRGNVV VTNIAQLVIY NVQPQDAGIY TCTARNVAGV LRADFPLSVV
310 320 330 340 350
RGGQARATSE SSLNGTAFPA TECLKPPDSE DCGEEQTRWH FDAQANNCLT
360 370 380 390 400
FTFGHCHHNL NHFETYEACM LACMSGPLAI CSLPALQGPC KAYVPRWAYN
410 420 430 440 450
SQTGLCQSFV YGGCEGNGNN FESREACEES CPFPRGNQHC RACKPRQKLV
460 470 480 490 500
TSFCRSDFVI LGRVSELTEE QDSGRALVTV DEVLKDEKMG LKFLGREPLE
510 520 530 540 550
VTLLHVDWTC PCPNVTVGET PLIIMGEVDG GMAMLRPDSF VGASSTRRVR
560 570
KLREVMYKKT CDVLKDFLGL Q
Length:571
Mass (Da):63,333
Last modified:October 23, 2007 - v2
Checksum:i3A75A449F53703AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti380I → T in AAP72503 (PubMed:12595574).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY308804 mRNA. Translation: AAP72503.1.
AL645846 Genomic DNA. Translation: CAI24703.1.
CCDSiCCDS25251.1.
RefSeqiNP_861540.2. NM_181819.2.
XP_006533561.1. XM_006533498.3.
XP_006533562.1. XM_006533499.3.
XP_006533563.1. XM_006533500.3.
XP_006533564.1. XM_006533501.3.
XP_006533565.1. XM_006533502.2.
XP_011247365.1. XM_011249063.2.
UniGeneiMm.342216.

Genome annotation databases

EnsembliENSMUST00000061469; ENSMUSP00000053238; ENSMUSG00000044177.
GeneIDi278507.
KEGGimmu:278507.
UCSCiuc007kyg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY308804 mRNA. Translation: AAP72503.1.
AL645846 Genomic DNA. Translation: CAI24703.1.
CCDSiCCDS25251.1.
RefSeqiNP_861540.2. NM_181819.2.
XP_006533561.1. XM_006533498.3.
XP_006533562.1. XM_006533499.3.
XP_006533563.1. XM_006533500.3.
XP_006533564.1. XM_006533501.3.
XP_006533565.1. XM_006533502.2.
XP_011247365.1. XM_011249063.2.
UniGeneiMm.342216.

3D structure databases

ProteinModelPortaliQ7TQN3.
SMRiQ7TQN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234985. 1 interactor.
STRINGi10090.ENSMUSP00000053238.

Protein family/group databases

MEROPSiI02.954.

PTM databases

PhosphoSitePlusiQ7TQN3.

Proteomic databases

PaxDbiQ7TQN3.
PRIDEiQ7TQN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061469; ENSMUSP00000053238; ENSMUSG00000044177.
GeneIDi278507.
KEGGimmu:278507.
UCSCiuc007kyg.2. mouse.

Organism-specific databases

CTDi124857.
MGIiMGI:2669209. Wfikkn2.

Phylogenomic databases

eggNOGiENOG410KDTI. Eukaryota.
ENOG410XRTD. LUCA.
GeneTreeiENSGT00740000114929.
HOGENOMiHOG000154855.
HOVERGENiHBG108676.
InParanoidiQ7TQN3.
OMAiCPNVCGT.
OrthoDBiEOG091G09P2.
PhylomeDBiQ7TQN3.
TreeFamiTF315349.

Miscellaneous databases

PROiQ7TQN3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044177.
GenevisibleiQ7TQN3. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
4.10.410.10. 2 hits.
4.10.75.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR002350. Kazal_dom.
IPR002223. Kunitz_BPTI.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR020901. Prtase_inh_Kunz-CS.
IPR008993. TIMP-like_OB-fold.
IPR008197. WAP_dom.
IPR033639. WFIKKN2.
[Graphical view]
PANTHERiPTHR10083:SF87. PTHR10083:SF87. 1 hit.
PfamiPF07679. I-set. 1 hit.
PF00014. Kunitz_BPTI. 2 hits.
PF01759. NTR. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00131. KU. 2 hits.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF50242. SSF50242. 1 hit.
SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 2 hits.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50189. NTR. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWFKN2_MOUSE
AccessioniPrimary (citable) accession number: Q7TQN3
Secondary accession number(s): Q5SUS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.