Reviewed, UniProtKB/Swiss-Prot Q7TQM4 (SOAT2_RAT)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 2
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 2
    Acyl coenzyme A:cholesterol acyltransferase 2
      Short name=ACAT-2

Gene names

Name:	Soat2
Synonyms:	Acat2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa By similarity.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subunit structure	May form homo- or heterodimers By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from direct assay. Source: RGD response to nutrient Inferred from expression pattern. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Sterol O-acyltransferase 2		PRO_0000255713
Regions
	Transmembrane	122 – 142	21	Potential
	Transmembrane	153 – 173	21	Potential
	Transmembrane	196 – 216	21	Potential
	Transmembrane	264 – 284	21	Potential
	Transmembrane	306 – 326	21	Potential
	Transmembrane	349 – 369	21	Potential
	Transmembrane	439 – 459	21	Potential
	Transmembrane	474 – 494	21	Potential
Sites
	Active site	436	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7TQM4-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 75CE7DE53F03C88A	FASTA	524	60,498
	10 20 30 40 50 60 MEPKAPQLRR RERQGEEQEN GACGEGNTRT HRAPDLVQWT RHMEAVKTQC LEQAQRELAE 70 80 90 100 110 120 LMDRAIWEAV QAYPKQDRPL PSTASDSTRK TQELHPGKRK VFITRKSLLD ELMGVQHFRT 130 140 150 160 170 180 IYHMFIAGLC VLIISTLAID FIDEGRLMLE FDLLLFSFGQ LPLALMMWVP MFLSTLLLPY 190 200 210 220 230 240 QTLRLWARPR SGGAWTLGAS LGCVLLAAHA AVLCVLPVHV SVKHELPPAS RCVLVFEQVR 250 260 270 280 290 300 FLMKSYSFLR ETVPGIFCVR GGKGICTPSF SSYLYFLFCP TLIYRETYPR TPSIRWNYVA 310 320 330 340 350 360 KNFAQALGCL LYACFILGRL CVPVFANMSR EPFSTRALLL SILHATGPGI FMLLLIFFAF 370 380 390 400 410 420 LHCWLNAFAE MLRFGDRMFY RDWWNSTSFS NYYRTWNVVV HDWLYSYVYQ DGLWLLGRQG 430 440 450 460 470 480 RGAAMLGVFL VSALVHEYIF CFVLGFFYPV MLILFLVVGG LLNFTMNDRH TGPAWNILMW 490 500 510 520 TFLFLGQGIQ VSLYCQEWYA RRHCPLPQPT FWELVTPRSW SCHP

References

[1]	"Acyl-coenzyme A:cholesterol acyltransferase 2 is responsible for elevated intestinal cholesterol ester formation in streptozotocin-induced diabetic rats." Horiuchi S., Hori M., Hakamata H., Sato M., Miyazaki A. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB101480 mRNA. Translation: BAC78210.1.
IPI	IPI00396902.
RefSeq	NP_714950.2.
UniGene	Rn.83628
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TQM4.
Genome annotation databases
Ensembl	ENSRNOT00000015368; ENSRNOP00000015367; ENSRNOG00000011163; Rattus norvegicus. [Genome view]
GeneID	266770.
KEGG	rno:266770.
UCSC	NM_153728. rat.
Organism-specific databases
CTD	266770.
RGD	628865. Soat2.
Phylogenomic databases
eggNOG	roNOG13755.
HOVERGEN	Q7TQM4.
InParanoid	Q7TQM4.
Enzyme and pathway databases
BRENDA	2.3.1.26. 248.
Gene expression databases
Genevestigator	Q7TQM4.
GermOnline	ENSRNOG00000011163. Rattus norvegicus.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB01094. Hesperetin.
NextBio	624637.

Entry information

Entry name

SOAT2_RAT

Accession

Primary (citable) accession number: Q7TQM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 1, 2003
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)