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Protein

Amphiphysin

Gene

Amph

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • learning Source: MGI
  • synaptic vesicle endocytosis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Amphiphysin
Gene namesi
Name:Amph
Synonyms:Amph1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:103574. Amph.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686AmphiphysinPRO_0000192948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei252 – 2521PhosphoserineCombined sources
Modified residuei260 – 2601PhosphothreonineCombined sources
Modified residuei262 – 2621PhosphoserineCombined sources
Modified residuei268 – 2681PhosphoserineCombined sources
Modified residuei272 – 2721PhosphoserineCombined sources
Modified residuei276 – 2761PhosphoserineCombined sources
Modified residuei280 – 2801PhosphothreonineCombined sources
Modified residuei500 – 5001PhosphoserineCombined sources
Modified residuei516 – 5161PhosphoserineBy similarity
Modified residuei518 – 5181PhosphoserineBy similarity
Modified residuei629 – 6291PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TQF7.
MaxQBiQ7TQF7.
PaxDbiQ7TQF7.
PeptideAtlasiQ7TQF7.
PRIDEiQ7TQF7.

PTM databases

iPTMnetiQ7TQF7.
PhosphoSiteiQ7TQF7.

Expressioni

Gene expression databases

BgeeiQ7TQF7.
CleanExiMM_AMPH.
ExpressionAtlasiQ7TQF7. baseline and differential.
GenevisibleiQ7TQF7. MM.

Interactioni

Subunit structurei

Heterodimer with BIN1. Binds SH3GLB1. Interacts with AP2B1 (By similarity). Interacts with REPS1 and SGIP1; may be involved in clathrin-mediated endocytosis (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dnm1P390533EBI-775139,EBI-397785
Dnm2P390545EBI-775139,EBI-642337
Dnm3Q8BZ982EBI-775139,EBI-6880033

Protein-protein interaction databases

BioGridi229989. 5 interactions.
IntActiQ7TQF7. 7 interactions.
MINTiMINT-1592321.
STRINGi10090.ENSMUSP00000003345.

Structurei

3D structure databases

ProteinModelPortaliQ7TQF7.
SMRiQ7TQF7. Positions 19-239, 609-685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 240217BARPROSITE-ProRule annotationAdd
BLAST
Domaini613 – 68674SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili10 – 8374Sequence analysisAdd
BLAST
Coiled coili144 – 19148Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi261 – 31151Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3771. Eukaryota.
ENOG410ZBW1. LUCA.
GeneTreeiENSGT00390000017588.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiQ7TQF7.
KOiK12562.
OrthoDBiEOG7XWPND.
PhylomeDBiQ7TQF7.
TreeFamiTF313542.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TQF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
60 70 80 90 100
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
110 120 130 140 150
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
160 170 180 190 200
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSSRVG
210 220 230 240 250
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFSIQGAPS
260 270 280 290 300
DSGPLRIAKT PSPPEEPSPL PSPTASPNHT LAPASPAPVR PRSPSQTRKG
310 320 330 340 350
PPVPPLPKVT PTKELKQENI INFFEDNFVP EINVTTPSQN EVLEVKKEET
360 370 380 390 400
LLDLDFDPFK PDVAPAGSAA ATHSPMSQTL PWDLWTTSTD LVQPASGGSF
410 420 430 440 450
NDFTQAQDTS LFTMQTDQNM AETEQALPTE PQAEEPPATA AAPTAGLDLG
460 470 480 490 500
LEMEEPKEEA VIPPATDTGE TVETAVPTEG APVEEAEAEK AALPAGEGGS
510 520 530 540 550
PEGAKIDGES TELAISESPQ PVEPEAGAPQ VIPSVVIEPA SNHEGEGEHQ
560 570 580 590 600
ETATGTEPRE AAEDVAAQGS AGEKQEVATE PTPLDSQATL PASAGAVDAS
610 620 630 640 650
LSAGDATQEL PPGFLYKVET LHDFEAANSD ELNLQRGDVV LVVPSDSEAD
660 670 680
QDAGWLVGVK ESDWLQYRDL ATYKGLFPEN FTRRLE
Length:686
Mass (Da):75,013
Last modified:October 1, 2003 - v1
Checksum:iD292E24653A442A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024817 mRNA. Translation: AAH24817.1.
BC054718 mRNA. Translation: AAH54718.1.
CCDSiCCDS26258.1.
RefSeqiNP_001276475.1. NM_001289546.1.
NP_778172.1. NM_175007.2.
UniGeneiMm.101650.

Genome annotation databases

EnsembliENSMUST00000003345; ENSMUSP00000003345; ENSMUSG00000021314.
GeneIDi218038.
KEGGimmu:218038.
UCSCiuc007pom.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024817 mRNA. Translation: AAH24817.1.
BC054718 mRNA. Translation: AAH54718.1.
CCDSiCCDS26258.1.
RefSeqiNP_001276475.1. NM_001289546.1.
NP_778172.1. NM_175007.2.
UniGeneiMm.101650.

3D structure databases

ProteinModelPortaliQ7TQF7.
SMRiQ7TQF7. Positions 19-239, 609-685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229989. 5 interactions.
IntActiQ7TQF7. 7 interactions.
MINTiMINT-1592321.
STRINGi10090.ENSMUSP00000003345.

PTM databases

iPTMnetiQ7TQF7.
PhosphoSiteiQ7TQF7.

Proteomic databases

EPDiQ7TQF7.
MaxQBiQ7TQF7.
PaxDbiQ7TQF7.
PeptideAtlasiQ7TQF7.
PRIDEiQ7TQF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003345; ENSMUSP00000003345; ENSMUSG00000021314.
GeneIDi218038.
KEGGimmu:218038.
UCSCiuc007pom.2. mouse.

Organism-specific databases

CTDi273.
MGIiMGI:103574. Amph.

Phylogenomic databases

eggNOGiKOG3771. Eukaryota.
ENOG410ZBW1. LUCA.
GeneTreeiENSGT00390000017588.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiQ7TQF7.
KOiK12562.
OrthoDBiEOG7XWPND.
PhylomeDBiQ7TQF7.
TreeFamiTF313542.

Miscellaneous databases

PROiQ7TQF7.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TQF7.
CleanExiMM_AMPH.
ExpressionAtlasiQ7TQF7. baseline and differential.
GenevisibleiQ7TQF7. MM.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR003017. Amphiphysin_1.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF03114. BAR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
PR01252. AMPHIPHYSIN1.
PR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Eye.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252; THR-260; SER-262; SER-268; SER-272; SER-276; THR-280; SER-500 AND SER-629, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue and Lung.

Entry informationi

Entry nameiAMPH_MOUSE
AccessioniPrimary (citable) accession number: Q7TQF7
Secondary accession number(s): Q8R1C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: October 1, 2003
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.