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Protein

DnaJ homolog subfamily C member 2

Gene

Dnajc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3' (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chaperone, Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 2
Alternative name(s):
Gliosarcoma-related antigen MIDA1
Zuotin-related factor 1
Cleaved into the following chain:
Gene namesi
Name:Dnajc2
Synonyms:Mida1, Zrf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620524. Dnajc2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002801791 – 621DnaJ homolog subfamily C member 2Add BLAST621
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004257552 – 621DnaJ homolog subfamily C member 2, N-terminally processedAdd BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei47PhosphoserineCombined sources1
Modified residuei49PhosphoserineBy similarity1
Modified residuei60PhosphoserineCombined sources1
Modified residuei63PhosphoserineBy similarity1
Modified residuei183PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in M (mitotic) phase.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7TQ20.
PRIDEiQ7TQ20.

PTM databases

iPTMnetiQ7TQ20.
PhosphoSitePlusiQ7TQ20.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012392.

Interactioni

Subunit structurei

Interacts (via ZRF1-UBD region) with ID1. Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016909.

Structurei

3D structure databases

ProteinModelPortaliQ7TQ20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 161JPROSITE-ProRule annotationAdd BLAST74
Domaini449 – 511SANT 1PROSITE-ProRule annotationAdd BLAST63
Domaini549 – 604SANT 2PROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 250ZRF1-UBDAdd BLAST91

Domaini

The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub (By similarity).By similarity

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiQ7TQ20.
KOiK09522.
PhylomeDBiQ7TQ20.
TreeFamiTF105834.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TQ20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLPSAAEG QGTAITHALT SASAVCQVEP VGRWFEAFVK RRNRNASTSF
60 70 80 90 100
QELEDKKELS EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY
110 120 130 140 150
KATQRQIKAA HKTMVLKHHP DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD
160 170 180 190 200
PVKRRAFNSV DPTFDNSVPS KSEAKENFFQ VFSPVFERNS RWSNKKNVPK
210 220 230 240 250
LGDMNSSFED VDAFYSFWYN FDSWREFSYL DEEEKEKAEC RDERKWIEKQ
260 270 280 290 300
NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEGKAK KEAEKRAKAE
310 320 330 340 350
ARRKEQEAKE KQRQAELEAV RLAKEKEEEE VRQQALLAKK EKEIQKKAIK
360 370 380 390 400
KERQKLRNSC KNWNHFSDNE ADRVKMMEEV EKLCDRLELA SLQCLNEILA
410 420 430 440 450
SSTREVGKAA LEKQIEEVNE LMRKEKEEAD ARMRQASKNA EKSTGGSGSG
460 470 480 490 500
SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI
510 520 530 540 550
GKAKSLQKLD PHQKDDINKK AFDKFKKEHG VAPQADSAAP SERFEGPCID
560 570 580 590 600
SIPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMRRYKELV
610 620
EMVKAKKAAQ EQVLNASRAR K
Length:621
Mass (Da):71,769
Last modified:October 1, 2003 - v1
Checksum:i06ED1C7AA56267A6
GO
Isoform 2 (identifier: Q7TQ20-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:547
Mass (Da):63,504
Checksum:i2F9494AF077A0AC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti446G → R in AAH88838 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0407201 – 74Missing in isoform 2. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY322161 mRNA. Translation: AAP84338.1.
AY322163 mRNA. Translation: AAP84340.1.
BC088838 mRNA. Translation: AAH88838.1.
AF118853 mRNA. Translation: AAD45407.1.
RefSeqiNP_446228.2. NM_053776.2.
XP_008760850.1. XM_008762628.2.
UniGeneiRn.11908.

Genome annotation databases

GeneIDi116456.
KEGGirno:116456.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY322161 mRNA. Translation: AAP84338.1.
AY322163 mRNA. Translation: AAP84340.1.
BC088838 mRNA. Translation: AAH88838.1.
AF118853 mRNA. Translation: AAD45407.1.
RefSeqiNP_446228.2. NM_053776.2.
XP_008760850.1. XM_008762628.2.
UniGeneiRn.11908.

3D structure databases

ProteinModelPortaliQ7TQ20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016909.

PTM databases

iPTMnetiQ7TQ20.
PhosphoSitePlusiQ7TQ20.

Proteomic databases

PaxDbiQ7TQ20.
PRIDEiQ7TQ20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116456.
KEGGirno:116456.

Organism-specific databases

CTDi27000.
RGDi620524. Dnajc2.

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiQ7TQ20.
KOiK09522.
PhylomeDBiQ7TQ20.
TreeFamiTF105834.

Miscellaneous databases

PROiQ7TQ20.

Gene expression databases

BgeeiENSRNOG00000012392.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJC2_RAT
AccessioniPrimary (citable) accession number: Q7TQ20
Secondary accession number(s): Q5HZY5, Q7TQ18, Q9WVG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.