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Protein

DNA polymerase nu

Gene

Poln

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase nu (EC:2.7.7.7)
Gene namesi
Name:Poln
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2675617. Poln.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864DNA polymerase nuPRO_0000227939Add
BLAST

Proteomic databases

PaxDbiQ7TQ07.
PRIDEiQ7TQ07.

PTM databases

PhosphoSiteiQ7TQ07.

Expressioni

Tissue specificityi

Expressed only in testis namely in primary spermatocytes and in round spermatids.1 Publication

Gene expression databases

BgeeiQ7TQ07.
CleanExiMM_POLN.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036110.

Structurei

3D structure databases

ProteinModelPortaliQ7TQ07.
SMRiQ7TQ07. Positions 195-858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGiENOG410IPH2. Eukaryota.
COG0749. LUCA.
HOGENOMiHOG000230904.
HOVERGENiHBG079981.
InParanoidiQ7TQ07.
KOiK16618.
OrthoDBiEOG7QG43B.
PhylomeDBiQ7TQ07.
TreeFamiTF337202.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TQ07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENYEACVGF DVCEIPLSAV AQKIMSAMRS GDFMDSRNEG ESTNTSKVAK
60 70 80 90 100
KSSVHYSVLA EHEETQSLGT KNPESLITQT PRGSIELCPQ PSITKLTCQL
110 120 130 140 150
SAGQVQNSIS SLGLSSYLIP QCDQEASVLP NMEHKRQHFL KENIGKEDKD
160 170 180 190 200
NSSLKRKYIT CSKSSEKASK HTALEKDTDG TESWPNSRDT RALGERLCDV
210 220 230 240 250
RYLGDLAKAQ LMDALKQAAA LVVTLMYKDG STQLSAKEAL TCTVKGIVVL
260 270 280 290 300
LKSHVGNSTL TLPAHGGALE KDFISEDHCV YIHTEHSPFW DPKQEAHSLF
310 320 330 340 350
VRNILFWTLR CKCPVVCFNA KDFVRTVLQL YGEDGSWKHV ADFVGLDPRV
360 370 380 390 400
AAWLIDPSDT APSFEDLVAK HLEKSITVKP SSTFREASRN TLSQNVFMNL
410 420 430 440 450
KILYDLTMDL CSKLKAYGLW QLFCTLELPL IPILAVMENH KIPVDKEEME
460 470 480 490 500
RTSALLGARL KELEQEAHFV AGEQFLIMSN NQLREILFGK LKLHLLSQRK
510 520 530 540 550
HLPRTGLQNQ LSTSEAMLNS LQDLHPLPKL ILEYRQVHKI KSTFIDGLLA
560 570 580 590 600
YMKKGSISST WNQTGTVTGR LSAKHPNIQG ISKHPIKISK PWNFKGKEEE
610 620 630 640 650
TVTISPRTLF VSSEGHTFLA ADFSQIELRI LAHLSGDPEL LKLFQESERD
660 670 680 690 700
DVFSTLTSQW KDIPIERVTH MDREQTKKVV YSVVYGAGKE RLAACLGVTV
710 720 730 740 750
LEATHFLERF LQKYKKIKDF AQTVIGQCHS AGYVTSILGR RRPLPRICAQ
760 770 780 790 800
DQQLRAQAER QAVNFVVQGS AADLCKLAMI RISTAVATSP TLTARLVAQI
810 820 830 840 850
HDELLFEVED TQVPEFAALV RRIMESLQQV QTLELQLQVP LKVNLSVGRS
860
WGHLTPLQEI LGSA
Length:864
Mass (Da):96,675
Last modified:March 21, 2006 - v2
Checksum:i37F5246FC4EEF3FD
GO
Isoform 2 (identifier: Q7TQ07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     689-732: Missing.

Note: No experimental confirmation available.
Show »
Length:820
Mass (Da):91,743
Checksum:iECC8ABE88F165FB4
GO

Sequence cautioni

The sequence AAN39837.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE21491.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei689 – 73244Missing in isoform 2. 1 PublicationVSP_017616Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135562 mRNA. Translation: AAN39837.2. Different initiation.
AK133063 mRNA. Translation: BAE21491.1. Different initiation.
RefSeqiNP_001276732.1. NM_001289803.1.
NP_001276733.1. NM_001289804.1.
NP_862905.1. NM_181857.4.
UniGeneiMm.319286.

Genome annotation databases

GeneIDi272158.
KEGGimmu:272158.
UCSCiuc008xbt.3. mouse. [Q7TQ07-1]
uc008xbu.3. mouse. [Q7TQ07-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135562 mRNA. Translation: AAN39837.2. Different initiation.
AK133063 mRNA. Translation: BAE21491.1. Different initiation.
RefSeqiNP_001276732.1. NM_001289803.1.
NP_001276733.1. NM_001289804.1.
NP_862905.1. NM_181857.4.
UniGeneiMm.319286.

3D structure databases

ProteinModelPortaliQ7TQ07.
SMRiQ7TQ07. Positions 195-858.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036110.

PTM databases

PhosphoSiteiQ7TQ07.

Proteomic databases

PaxDbiQ7TQ07.
PRIDEiQ7TQ07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi272158.
KEGGimmu:272158.
UCSCiuc008xbt.3. mouse. [Q7TQ07-1]
uc008xbu.3. mouse. [Q7TQ07-2]

Organism-specific databases

CTDi353497.
MGIiMGI:2675617. Poln.

Phylogenomic databases

eggNOGiENOG410IPH2. Eukaryota.
COG0749. LUCA.
HOGENOMiHOG000230904.
HOVERGENiHBG079981.
InParanoidiQ7TQ07.
KOiK16618.
OrthoDBiEOG7QG43B.
PhylomeDBiQ7TQ07.
TreeFamiTF337202.

Miscellaneous databases

NextBioi393551.
PROiQ7TQ07.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TQ07.
CleanExiMM_POLN.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308."
    Marini F., Kim N., Schuffert A., Wood R.D.
    J. Biol. Chem. 278:32014-32019(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.

Entry informationi

Entry nameiDPOLN_MOUSE
AccessioniPrimary (citable) accession number: Q7TQ07
Secondary accession number(s): Q3V0L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: May 11, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.