Q7TPS5 (C2CD5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C2 domain-containing protein 5

Alternative name(s):
138 kDa C2 domain-containing phosphoprotein

Gene names

Name:	C2cd5
Synonyms:	Cdp138, Kiaa0528

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1016 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM. Ref.6
Cofactor	Binds 2 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subcellular location	Cytoplasmic vesicle membrane By similarity. Cytoplasm › cell cortex By similarity. Cell membrane By similarity. Cell projection › ruffle By similarity. Note: Dynamically associated with GLUT4-containing glucose storage vesicles (GSV) and plasma membrane in response to insulin stimulation By similarity.
Tissue specificity	Expressed in liver, muscle and fat.
Domain	The C2 domain binds to calcium and membrane lipids By similarity.
Post-translational modification	Phosphorylated on Ser-197 by active myristoylated kinase AKT2; insulin-stimulated phosphorylation by AKT2 regulates SLC2A4/GLUT4 translocation into the plasma membrane By similarity.
Sequence similarities	Contains 1 C2 domain.
Sequence caution	The sequence BAD32244.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calcium Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway via phosphatidylinositol 3-kinase cascade Inferred from mutant phenotype Ref.6. Source: UniProtKB intracellular protein transmembrane transport Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of glucose import in response to insulin stimulus Inferred from mutant phenotype PubMed 21907141. Source: MGI positive regulation of protein targeting to membrane Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of vesicle fusion Inferred from mutant phenotype Ref.6. Source: UniProtKB protein localization to plasma membrane Inferred from mutant phenotype PubMed 21907141. Source: MGI vesicle fusion Inferred from mutant phenotype PubMed 21907141. Source: MGI
Cellular_component	cell cortex Inferred from sequence or structural similarity. Source: UniProtKB cytoplasmic vesicle membrane Inferred from sequence or structural similarity. Source: UniProtKB ruffle membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from direct assay PubMed 21907141. Source: MGI calcium-dependent phospholipid binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TPS5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TPS5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     268-316: IPFNEDPNPN...SDTDLSLTPK → SPLVHPPSHG...FSVSVPTLIY
     845-861: Missing.

Isoform 3 (identifier: Q7TPS5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1016	1016	C2 domain-containing protein 5		PRO_0000247451
Regions
	Domain	1 – 90	90	C2
	Calcium binding	13 – 27	15	1; low affinity By similarity
	Calcium binding	75 – 86	12	2; high affinity By similarity
Amino acid modifications
	Modified residue	197	1	Phosphoserine; by PKB/AKT2 By similarity
	Modified residue	200	1	Phosphoserine By similarity
	Modified residue	260	1	Phosphoserine Ref.5
	Modified residue	262	1	Phosphoserine Ref.5
	Modified residue	293	1	Phosphoserine By similarity
	Modified residue	295	1	Phosphoserine By similarity
	Modified residue	317	1	Phosphothreonine By similarity
	Modified residue	601	1	Phosphothreonine By similarity
	Modified residue	643	1	Phosphoserine By similarity
	Modified residue	659	1	Phosphoserine Ref.4
	Modified residue	869	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 198	198	Missing in isoform 3.		VSP_019991
	Alternative sequence	268 – 316	49	IPFNE…SLTPK → SPLVHPPSHGCRSTHNSPIH TATGSRLTQNFSVSVPTLIY in isoform 2.		VSP_019992
	Alternative sequence	845 – 861	17	Missing in isoform 2.		VSP_019993
Experimental info
	Sequence conflict	11	1	A → S in BAC26634. Ref.2
	Sequence conflict	590	1	P → T in BAC26634. Ref.2
	Sequence conflict	632	1	T → S in BAD32244. Ref.1
	Sequence conflict	632	1	T → S in AAH27763. Ref.3
	Sequence conflict	632	1	T → S in AAH49905. Ref.3
	Sequence conflict	793	1	A → V in AAH53913. Ref.3
	Sequence conflict	868	1	A → T in BAB30814. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: A1E3614203DFCD03	FASTA	1,016	111,664
	10 20 30 40 50 60 MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ WNSEWFKFEV 70 80 90 100 110 120 DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE AATVISGWFP IYDTIHGIRG 130 140 150 160 170 180 EINVVVKVDL FNDSNRFRQS SCGVKFFCTT SIPKCYRAVV IHGFVEELVV NEDPEYQWID 190 200 210 220 230 240 RIRTPRASNE ARQRLISLMS GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG 250 260 270 280 290 300 TACTLDKLSS PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY 310 320 330 340 350 360 SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF LTLTAFPPGL 370 380 390 400 410 420 LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK SHAKALGCHA VVGYSESTSI 430 440 450 460 470 480 CEEVCILSAS GTAAVLNPRF LQEGTVEGCL EQRIEENLPV GCGFCHIPYD ELNMPFPAHL 490 500 510 520 530 540 TYCYNCRKQK VPDVLFTTID LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF 550 560 570 580 590 600 MEYEVHTQLM NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK 610 620 630 640 650 660 TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ RSRLLRSQSE 670 680 690 700 710 720 SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP PSGFYSCNTE IMPGINNWTS 730 740 750 760 770 780 EIQMFTSVRV VRLSSLNLTN QALNKNFNGL CENLLKSLYF KLRSMTPCCL CHVNFTVSLP 790 800 810 820 830 840 EDELIQVTVT AVAITFDKNQ ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF 850 860 870 880 890 900 PAAKEHLESA NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI 910 920 930 940 950 960 PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA LGGNAVVSYI 970 980 990 1000 1010 MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ QPAANCQPSC TGEVTT
	Isoform 2 [UniParc]. Checksum: BEE3F6FFFB05366F	FASTA	990	108,869
	10 20 30 40 50 60 MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ WNSEWFKFEV 70 80 90 100 110 120 DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE AATVISGWFP IYDTIHGIRG 130 140 150 160 170 180 EINVVVKVDL FNDSNRFRQS SCGVKFFCTT SIPKCYRAVV IHGFVEELVV NEDPEYQWID 190 200 210 220 230 240 RIRTPRASNE ARQRLISLMS GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG 250 260 270 280 290 300 TACTLDKLSS PAAFLPACSS PSRELKESPL VHPPSHGCRS THNSPIHTAT GSRLTQNFSV 310 320 330 340 350 360 SVPTLIYTGM GSGSAGKEGG PFKALLRQQT QSALEQREFP FLTLTAFPPG LLVHVGGVVS 370 380 390 400 410 420 ARSVKLLDRI HNPDEPETRD AWWAEIRQEI KSHAKALGCH AVVGYSESTS ICEEVCILSA 430 440 450 460 470 480 SGTAAVLNPR FLQEGTVEGC LEQRIEENLP VGCGFCHIPY DELNMPFPAH LTYCYNCRKQ 490 500 510 520 530 540 KVPDVLFTTI DLPTDAVVVG KGCLIQARLC RLKKKAQAEA NATAISNLLP FMEYEVHTQL 550 560 570 580 590 600 MNKLKLKGMN ALFGLRIQIT VGETMLMGLA SATGVYLAAL PTPGGIQIAG KTPNDGSYEQ 610 620 630 640 650 660 HISHMQKRIN DTIAKNKELY EITPPEVSEE MIGSPIPEPR QRSRLLRSQS ESSDEVTELD 670 680 690 700 710 720 LSHGKKDAFV LEIDDTDAME DVHSLLTDAP PPSGFYSCNT EIMPGINNWT SEIQMFTSVR 730 740 750 760 770 780 VVRLSSLNLT NQALNKNFNG LCENLLKSLY FKLRSMTPCC LCHVNFTVSL PEDELIQVTV 790 800 810 820 830 840 TAVAITFDKN QALQTTKPHV EKSLQRASTD NEELLQFPLE LCSDSLPPHP FPAAKAVTVE 850 860 870 880 890 900 KASAMGDGNF RNRSAPPCAS PTVGVVKMTP LSFIPGAKIT KYLGIINMFF IRETTSLREE 910 920 930 940 950 960 GGVSGFLHAF IAEVFAMVRA HVAALGGNAV VSYIMKQCVF MENPSKNQAQ CLINVSGDAV 970 980 990 VFVRDSDLEV MSSQQPAANC QPSCTGEVTT
	Isoform 3 [UniParc]. Checksum: BD6948023B725703	FASTA	818	88,974
	10 20 30 40 50 60 MSGELQRKIG LKVLEMRGNA VVGYLQCFDL EGESGLVVRA IGTACTLDKL SSPAAFLPAC 70 80 90 100 110 120 SSPSRELKEI PFNEDPNPNT HSSGPSTPLK NQTYSFSPSK SYSRQSSSSD TDLSLTPKTG 130 140 150 160 170 180 MGSGSAGKEG GPFKALLRQQ TQSALEQREF PFLTLTAFPP GLLVHVGGVV SARSVKLLDR 190 200 210 220 230 240 IHNPDEPETR DAWWAEIRQE IKSHAKALGC HAVVGYSEST SICEEVCILS ASGTAAVLNP 250 260 270 280 290 300 RFLQEGTVEG CLEQRIEENL PVGCGFCHIP YDELNMPFPA HLTYCYNCRK QKVPDVLFTT 310 320 330 340 350 360 IDLPTDAVVV GKGCLIQARL CRLKKKAQAE ANATAISNLL PFMEYEVHTQ LMNKLKLKGM 370 380 390 400 410 420 NALFGLRIQI TVGETMLMGL ASATGVYLAA LPTPGGIQIA GKTPNDGSYE QHISHMQKRI 430 440 450 460 470 480 NDTIAKNKEL YEITPPEVSE EMIGSPIPEP RQRSRLLRSQ SESSDEVTEL DLSHGKKDAF 490 500 510 520 530 540 VLEIDDTDAM EDVHSLLTDA PPPSGFYSCN TEIMPGINNW TSEIQMFTSV RVVRLSSLNL 550 560 570 580 590 600 TNQALNKNFN GLCENLLKSL YFKLRSMTPC CLCHVNFTVS LPEDELIQVT VTAVAITFDK 610 620 630 640 650 660 NQALQTTKPH VEKSLQRAST DNEELLQFPL ELCSDSLPPH PFPAAKEHLE SANSNSGIPA 670 680 690 700 710 720 AQRAVTVEKA SAMGDGNFRN RSAPPCASPT VGVVKMTPLS FIPGAKITKY LGIINMFFIR 730 740 750 760 770 780 ETTSLREEGG VSGFLHAFIA EVFAMVRAHV AALGGNAVVS YIMKQCVFME NPSKNQAQCL 790 800 810 INVSGDAVVF VRDSDLEVMS SQQPAANCQP SCTGEVTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Fetal brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 710-1016 (ISOFORM 2). Strain: C57BL/6J. Tissue: Egg, Embryo and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 513-1016 (ISOFORM 2). Strain: C57BL/6J. Tissue: Egg and Mammary tumor.
[4]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-659, MASS SPECTROMETRY. Tissue: Forebrain.
[5]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-260 AND SER-262, MASS SPECTROMETRY. Tissue: Brain cortex.
[6]	"C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane." Xie X., Gong Z., Mansuy-Aubert V., Zhou Q.L., Tatulian S.A., Sehrt D., Gnad F., Brill L.M., Motamedchaboki K., Chen Y., Czech M.P., Mann M., Kruger M., Jiang Z.Y. Cell Metab. 14:378-389(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK172966 mRNA. Translation: BAD32244.1. Different initiation. AK017577 mRNA. Translation: BAB30814.1. AK029825 mRNA. Translation: BAC26634.1. AK163233 mRNA. Translation: BAE37246.1. BC027763 mRNA. Translation: AAH27763.1. BC049905 mRNA. Translation: AAH49905.1. BC053913 mRNA. Translation: AAH53913.1.
IPI	IPI00408171. IPI00462743. IPI00776032.
RefSeq	NP_001103158.1. NM_001109688.1. NP_083357.2. NM_029081.2. NP_084173.1. NM_029897.1.
UniGene	Mm.130260.
3D structure databases
ProteinModelPortal	Q7TPS5.
SMR	Q7TPS5. Positions 3-91.
ModBase	Search...
PTM databases
PhosphoSite	Q7TPS5.
Proteomic databases
PaxDb	Q7TPS5.
PRIDE	Q7TPS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279.
GeneID	74741.
KEGG	mmu:74741.
UCSC	uc009epx.2. mouse. uc009epy.2. mouse.
Organism-specific databases
CTD	9847.
MGI	MGI:1921991. C2cd5.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG235531.
GeneTree	ENSGT00390000000212.
HOVERGEN	HBG081824.
InParanoid	Q7TPS5.
OMA	MFTQVWR.
OrthoDB	EOG4C2H8T.
Gene expression databases
ArrayExpress	Q7TPS5.
Bgee	Q7TPS5.
CleanEx	MM_5730419I09RIK.
Genevestigator	Q7TPS5.
GermOnline	ENSMUSG00000030279. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. [Graphical view]
Pfam	PF00168. C2. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	341536.
SOURCE	Search...

Entry information

Entry name

C2CD5_MOUSE

Accession

Primary (citable) accession number: Q7TPS5
Secondary accession number(s): Q3TQY6 , Q6A052, Q80VA1, Q8C0U3, Q8CID5, Q9CS85

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 29, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program