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Protein

C2 domain-containing protein 5

Gene

C2cd5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.1 Publication

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. The ions are bound to the C2 domains.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi13 – 271; low affinityBy similarityAdd BLAST15
Calcium bindingi75 – 862; high affinityBy similarityAdd BLAST12

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: MGI

GO - Biological processi

  • cellular response to insulin stimulus Source: UniProtKB
  • insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: UniProtKB
  • intracellular protein transmembrane transport Source: UniProtKB
  • membrane organization Source: Reactome
  • positive regulation of glucose import in response to insulin stimulus Source: MGI
  • positive regulation of glucose transport Source: UniProtKB
  • positive regulation of protein targeting to membrane Source: UniProtKB
  • positive regulation of vesicle fusion Source: UniProtKB
  • protein localization to plasma membrane Source: MGI
  • vesicle fusion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
C2 domain-containing protein 5
Alternative name(s):
138 kDa C2 domain-containing phosphoprotein
Gene namesi
Name:C2cd5
Synonyms:Cdp138, Kiaa0528
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1921991. C2cd5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002474511 – 1016C2 domain-containing protein 5Add BLAST1016

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197Phosphoserine; by PKB/AKT2By similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei260PhosphoserineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei304PhosphoserineCombined sources1
Modified residuei305PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Modified residuei317PhosphothreonineBy similarity1
Modified residuei323PhosphoserineBy similarity1
Modified residuei601PhosphothreonineBy similarity1
Modified residuei643PhosphoserineBy similarity1
Modified residuei657PhosphoserineCombined sources1
Modified residuei659PhosphoserineCombined sources1
Modified residuei661PhosphoserineCombined sources1
Modified residuei662PhosphoserineCombined sources1
Modified residuei666PhosphothreonineBy similarity1
Modified residuei671PhosphoserineBy similarity1
Modified residuei817PhosphoserineCombined sources1
Modified residuei869PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-197 by active myristoylated kinase AKT2; insulin-stimulated phosphorylation by AKT2 regulates SLC2A4/GLUT4 translocation into the plasma membrane.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TPS5.
PeptideAtlasiQ7TPS5.
PRIDEiQ7TPS5.

PTM databases

iPTMnetiQ7TPS5.
PhosphoSitePlusiQ7TPS5.

Expressioni

Tissue specificityi

Expressed in liver, muscle and fat.

Gene expression databases

BgeeiENSMUSG00000030279.
CleanExiMM_5730419I09RIK.
ExpressionAtlasiQ7TPS5. baseline and differential.
GenevisibleiQ7TPS5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107388.

Structurei

3D structure databases

ProteinModelPortaliQ7TPS5.
SMRiQ7TPS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90C2PROSITE-ProRule annotationAdd BLAST90

Domaini

The C2 domain binds to calcium and membrane lipids.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1031. Eukaryota.
ENOG410XQDE. LUCA.
GeneTreeiENSGT00390000000212.
HOVERGENiHBG081824.
InParanoidiQ7TPS5.
OrthoDBiEOG091G0FX6.
PhylomeDBiQ7TPS5.
TreeFamiTF323431.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TPS5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ
60 70 80 90 100
WNSEWFKFEV DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE
110 120 130 140 150
AATVISGWFP IYDTIHGIRG EINVVVKVDL FNDSNRFRQS SCGVKFFCTT
160 170 180 190 200
SIPKCYRAVV IHGFVEELVV NEDPEYQWID RIRTPRASNE ARQRLISLMS
210 220 230 240 250
GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG TACTLDKLSS
260 270 280 290 300
PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY
310 320 330 340 350
SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF
360 370 380 390 400
LTLTAFPPGL LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK
410 420 430 440 450
SHAKALGCHA VVGYSESTSI CEEVCILSAS GTAAVLNPRF LQEGTVEGCL
460 470 480 490 500
EQRIEENLPV GCGFCHIPYD ELNMPFPAHL TYCYNCRKQK VPDVLFTTID
510 520 530 540 550
LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF MEYEVHTQLM
560 570 580 590 600
NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK
610 620 630 640 650
TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ
660 670 680 690 700
RSRLLRSQSE SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP
710 720 730 740 750
PSGFYSCNTE IMPGINNWTS EIQMFTSVRV VRLSSLNLTN QALNKNFNGL
760 770 780 790 800
CENLLKSLYF KLRSMTPCCL CHVNFTVSLP EDELIQVTVT AVAITFDKNQ
810 820 830 840 850
ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF PAAKEHLESA
860 870 880 890 900
NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI
910 920 930 940 950
PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA
960 970 980 990 1000
LGGNAVVSYI MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ
1010
QPAANCQPSC TGEVTT
Length:1,016
Mass (Da):111,664
Last modified:July 25, 2006 - v2
Checksum:iA1E3614203DFCD03
GO
Isoform 2 (identifier: Q7TPS5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-316: IPFNEDPNPN...SDTDLSLTPK → SPLVHPPSHG...FSVSVPTLIY
     845-861: Missing.

Show »
Length:990
Mass (Da):108,869
Checksum:iBEE3F6FFFB05366F
GO
Isoform 3 (identifier: Q7TPS5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Note: No experimental confirmation available.
Show »
Length:818
Mass (Da):88,974
Checksum:iBD6948023B725703
GO

Sequence cautioni

The sequence BAD32244 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → S in BAC26634 (PubMed:16141072).Curated1
Sequence conflicti590P → T in BAC26634 (PubMed:16141072).Curated1
Sequence conflicti632T → S in BAD32244 (PubMed:15368895).Curated1
Sequence conflicti632T → S in AAH27763 (PubMed:15489334).Curated1
Sequence conflicti632T → S in AAH49905 (PubMed:15489334).Curated1
Sequence conflicti793A → V in AAH53913 (PubMed:15489334).Curated1
Sequence conflicti868A → T in BAB30814 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0199911 – 198Missing in isoform 3. 1 PublicationAdd BLAST198
Alternative sequenceiVSP_019992268 – 316IPFNE…SLTPK → SPLVHPPSHGCRSTHNSPIH TATGSRLTQNFSVSVPTLIY in isoform 2. 3 PublicationsAdd BLAST49
Alternative sequenceiVSP_019993845 – 861Missing in isoform 2. 3 PublicationsAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172966 mRNA. Translation: BAD32244.1. Different initiation.
AK017577 mRNA. Translation: BAB30814.1.
AK029825 mRNA. Translation: BAC26634.1.
AK163233 mRNA. Translation: BAE37246.1.
BC027763 mRNA. Translation: AAH27763.1.
BC049905 mRNA. Translation: AAH49905.1.
BC053913 mRNA. Translation: AAH53913.1.
CCDSiCCDS51952.1. [Q7TPS5-1]
CCDS51953.1. [Q7TPS5-2]
RefSeqiNP_001103158.1. NM_001109688.2. [Q7TPS5-2]
NP_001273507.1. NM_001286578.1. [Q7TPS5-3]
NP_083357.2. NM_029081.3. [Q7TPS5-1]
NP_084173.1. NM_029897.2.
UniGeneiMm.130260.

Genome annotation databases

EnsembliENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000203187; ENSMUSP00000145373; ENSMUSG00000030279. [Q7TPS5-1]
GeneIDi74741.
KEGGimmu:74741.
UCSCiuc009epx.3. mouse. [Q7TPS5-2]
uc009epy.3. mouse. [Q7TPS5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172966 mRNA. Translation: BAD32244.1. Different initiation.
AK017577 mRNA. Translation: BAB30814.1.
AK029825 mRNA. Translation: BAC26634.1.
AK163233 mRNA. Translation: BAE37246.1.
BC027763 mRNA. Translation: AAH27763.1.
BC049905 mRNA. Translation: AAH49905.1.
BC053913 mRNA. Translation: AAH53913.1.
CCDSiCCDS51952.1. [Q7TPS5-1]
CCDS51953.1. [Q7TPS5-2]
RefSeqiNP_001103158.1. NM_001109688.2. [Q7TPS5-2]
NP_001273507.1. NM_001286578.1. [Q7TPS5-3]
NP_083357.2. NM_029081.3. [Q7TPS5-1]
NP_084173.1. NM_029897.2.
UniGeneiMm.130260.

3D structure databases

ProteinModelPortaliQ7TPS5.
SMRiQ7TPS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107388.

PTM databases

iPTMnetiQ7TPS5.
PhosphoSitePlusiQ7TPS5.

Proteomic databases

PaxDbiQ7TPS5.
PeptideAtlasiQ7TPS5.
PRIDEiQ7TPS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000203187; ENSMUSP00000145373; ENSMUSG00000030279. [Q7TPS5-1]
GeneIDi74741.
KEGGimmu:74741.
UCSCiuc009epx.3. mouse. [Q7TPS5-2]
uc009epy.3. mouse. [Q7TPS5-1]

Organism-specific databases

CTDi9847.
MGIiMGI:1921991. C2cd5.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1031. Eukaryota.
ENOG410XQDE. LUCA.
GeneTreeiENSGT00390000000212.
HOVERGENiHBG081824.
InParanoidiQ7TPS5.
OrthoDBiEOG091G0FX6.
PhylomeDBiQ7TPS5.
TreeFamiTF323431.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

PROiQ7TPS5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030279.
CleanExiMM_5730419I09RIK.
ExpressionAtlasiQ7TPS5. baseline and differential.
GenevisibleiQ7TPS5. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC2CD5_MOUSE
AccessioniPrimary (citable) accession number: Q7TPS5
Secondary accession number(s): Q3TQY6
, Q6A052, Q80VA1, Q8C0U3, Q8CID5, Q9CS85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.