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Q7TPS5 (C2CD5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C2 domain-containing protein 5
Alternative name(s):
138 kDa C2 domain-containing phosphoprotein
Gene names
Name:C2cd5
Synonyms:Cdp138, Kiaa0528
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1016 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM. Ref.4

Cofactor

Binds 2 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subcellular location

Cytoplasmic vesicle membrane By similarity. Cytoplasmcell cortex By similarity. Cell membrane By similarity. Cell projectionruffle By similarity. Note: Dynamically associated with GLUT4-containing glucose storage vesicles (GSV) and plasma membrane in response to insulin stimulation By similarity.

Tissue specificity

Expressed in liver, muscle and fat.

Domain

The C2 domain binds to calcium and membrane lipids By similarity.

Post-translational modification

Phosphorylated on Ser-197 by active myristoylated kinase AKT2; insulin-stimulated phosphorylation by AKT2 regulates SLC2A4/GLUT4 translocation into the plasma membrane By similarity.

Sequence similarities

Contains 1 C2 domain.

Sequence caution

The sequence BAD32244.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
   LigandCalcium
Lipid-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to insulin stimulus

Inferred from direct assay Ref.4. Source: UniProtKB

insulin receptor signaling pathway via phosphatidylinositol 3-kinase

Inferred from mutant phenotype Ref.4. Source: UniProtKB

intracellular protein transmembrane transport

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of glucose import in response to insulin stimulus

Inferred from mutant phenotype PubMed 21907141. Source: MGI

positive regulation of glucose transport

Inferred from mutant phenotype Ref.4. Source: UniProtKB

positive regulation of protein targeting to membrane

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of vesicle fusion

Inferred from mutant phenotype Ref.4. Source: UniProtKB

protein localization to plasma membrane

Inferred from mutant phenotype PubMed 21907141. Source: MGI

vesicle fusion

Inferred from mutant phenotype PubMed 21907141. Source: MGI

   Cellular_componentcell cortex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 21907141. Source: MGI

ruffle membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncalcium ion binding

Inferred from direct assay PubMed 21907141. Source: MGI

calcium-dependent phospholipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7TPS5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7TPS5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     268-316: IPFNEDPNPN...SDTDLSLTPK → SPLVHPPSHG...FSVSVPTLIY
     845-861: Missing.
Isoform 3 (identifier: Q7TPS5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10161016C2 domain-containing protein 5
PRO_0000247451

Regions

Domain1 – 9090C2
Calcium binding13 – 27151; low affinity By similarity
Calcium binding75 – 86122; high affinity By similarity

Amino acid modifications

Modified residue1971Phosphoserine; by PKB/AKT2 By similarity
Modified residue2001Phosphoserine By similarity
Modified residue2931Phosphoserine By similarity
Modified residue2951Phosphoserine By similarity
Modified residue3171Phosphothreonine By similarity
Modified residue6011Phosphothreonine By similarity
Modified residue6431Phosphoserine By similarity
Modified residue6591Phosphoserine By similarity
Modified residue8691Phosphoserine By similarity

Natural variations

Alternative sequence1 – 198198Missing in isoform 3.
VSP_019991
Alternative sequence268 – 31649IPFNE…SLTPK → SPLVHPPSHGCRSTHNSPIH TATGSRLTQNFSVSVPTLIY in isoform 2.
VSP_019992
Alternative sequence845 – 86117Missing in isoform 2.
VSP_019993

Experimental info

Sequence conflict111A → S in BAC26634. Ref.2
Sequence conflict5901P → T in BAC26634. Ref.2
Sequence conflict6321T → S in BAD32244. Ref.1
Sequence conflict6321T → S in AAH27763. Ref.3
Sequence conflict6321T → S in AAH49905. Ref.3
Sequence conflict7931A → V in AAH53913. Ref.3
Sequence conflict8681A → T in BAB30814. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: A1E3614203DFCD03

FASTA1,016111,664
        10         20         30         40         50         60 
MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ WNSEWFKFEV 

        70         80         90        100        110        120 
DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE AATVISGWFP IYDTIHGIRG 

       130        140        150        160        170        180 
EINVVVKVDL FNDSNRFRQS SCGVKFFCTT SIPKCYRAVV IHGFVEELVV NEDPEYQWID 

       190        200        210        220        230        240 
RIRTPRASNE ARQRLISLMS GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG 

       250        260        270        280        290        300 
TACTLDKLSS PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY 

       310        320        330        340        350        360 
SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF LTLTAFPPGL 

       370        380        390        400        410        420 
LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK SHAKALGCHA VVGYSESTSI 

       430        440        450        460        470        480 
CEEVCILSAS GTAAVLNPRF LQEGTVEGCL EQRIEENLPV GCGFCHIPYD ELNMPFPAHL 

       490        500        510        520        530        540 
TYCYNCRKQK VPDVLFTTID LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF 

       550        560        570        580        590        600 
MEYEVHTQLM NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK 

       610        620        630        640        650        660 
TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ RSRLLRSQSE 

       670        680        690        700        710        720 
SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP PSGFYSCNTE IMPGINNWTS 

       730        740        750        760        770        780 
EIQMFTSVRV VRLSSLNLTN QALNKNFNGL CENLLKSLYF KLRSMTPCCL CHVNFTVSLP 

       790        800        810        820        830        840 
EDELIQVTVT AVAITFDKNQ ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF 

       850        860        870        880        890        900 
PAAKEHLESA NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI 

       910        920        930        940        950        960 
PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA LGGNAVVSYI 

       970        980        990       1000       1010 
MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ QPAANCQPSC TGEVTT 

« Hide

Isoform 2 [UniParc].

Checksum: BEE3F6FFFB05366F
Show »

FASTA990108,869
Isoform 3 [UniParc].

Checksum: BD6948023B725703
Show »

FASTA81888,974

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Fetal brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 710-1016 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Egg, Embryo and Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 513-1016 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Egg and Mammary tumor.
[4]"C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane."
Xie X., Gong Z., Mansuy-Aubert V., Zhou Q.L., Tatulian S.A., Sehrt D., Gnad F., Brill L.M., Motamedchaboki K., Chen Y., Czech M.P., Mann M., Kruger M., Jiang Z.Y.
Cell Metab. 14:378-389(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK172966 mRNA. Translation: BAD32244.1. Different initiation.
AK017577 mRNA. Translation: BAB30814.1.
AK029825 mRNA. Translation: BAC26634.1.
AK163233 mRNA. Translation: BAE37246.1.
BC027763 mRNA. Translation: AAH27763.1.
BC049905 mRNA. Translation: AAH49905.1.
BC053913 mRNA. Translation: AAH53913.1.
CCDSCCDS51952.1. [Q7TPS5-1]
CCDS51953.1. [Q7TPS5-2]
RefSeqNP_001103158.1. NM_001109688.2. [Q7TPS5-2]
NP_001273507.1. NM_001286578.1. [Q7TPS5-3]
NP_083357.2. NM_029081.3. [Q7TPS5-1]
NP_084173.1. NM_029897.2.
UniGeneMm.130260.

3D structure databases

ProteinModelPortalQ7TPS5.
SMRQ7TPS5. Positions 3-128.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ7TPS5.

Proteomic databases

MaxQBQ7TPS5.
PaxDbQ7TPS5.
PRIDEQ7TPS5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279. [Q7TPS5-1]
GeneID74741.
KEGGmmu:74741.
UCSCuc009epx.2. mouse. [Q7TPS5-2]
uc009epy.2. mouse. [Q7TPS5-1]

Organism-specific databases

CTD9847.
MGIMGI:1921991. C2cd5.
RougeSearch...

Phylogenomic databases

eggNOGNOG235531.
GeneTreeENSGT00390000000212.
HOVERGENHBG081824.
InParanoidQ7TPS5.
OMACQPSCTG.
OrthoDBEOG715Q38.
PhylomeDBQ7TPS5.
TreeFamTF323431.

Gene expression databases

BgeeQ7TPS5.
CleanExMM_5730419I09RIK.
GenevestigatorQ7TPS5.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
InterProIPR000008. C2_dom.
[Graphical view]
PfamPF00168. C2. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
PROSITEPS50004. C2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio341536.
SOURCESearch...

Entry information

Entry nameC2CD5_MOUSE
AccessionPrimary (citable) accession number: Q7TPS5
Secondary accession number(s): Q3TQY6 expand/collapse secondary AC list , Q6A052, Q80VA1, Q8C0U3, Q8CID5, Q9CS85
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot