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Q7TPS5

- C2CD5_MOUSE

UniProt

Q7TPS5 - C2CD5_MOUSE

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Protein

C2 domain-containing protein 5

Gene

C2cd5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.1 Publication

Cofactori

Binds 2 calcium ions per subunit. The ions are bound to the C2 domains (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi13 – 27151; low affinityBy similarityAdd
BLAST
Calcium bindingi75 – 86122; high affinityBy similarityAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: UniProtKB
  2. calcium ion binding Source: MGI

GO - Biological processi

  1. cellular response to insulin stimulus Source: UniProtKB
  2. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: UniProtKB
  3. intracellular protein transmembrane transport Source: UniProtKB
  4. positive regulation of glucose import in response to insulin stimulus Source: MGI
  5. positive regulation of glucose transport Source: UniProtKB
  6. positive regulation of protein targeting to membrane Source: UniProtKB
  7. positive regulation of vesicle fusion Source: UniProtKB
  8. protein localization to plasma membrane Source: MGI
  9. vesicle fusion Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
C2 domain-containing protein 5
Alternative name(s):
138 kDa C2 domain-containing phosphoprotein
Gene namesi
Name:C2cd5
Synonyms:Cdp138, Kiaa0528
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1921991. C2cd5.

Subcellular locationi

Cytoplasmic vesicle membrane By similarity. Cytoplasmcell cortex By similarity. Cell membrane By similarity. Cell projectionruffle By similarity
Note: Dynamically associated with GLUT4-containing glucose storage vesicles (GSV) and plasma membrane in response to insulin stimulation.By similarity

GO - Cellular componenti

  1. cell cortex Source: UniProtKB
  2. cytoplasmic vesicle membrane Source: UniProtKB
  3. plasma membrane Source: MGI
  4. ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10161016C2 domain-containing protein 5PRO_0000247451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei197 – 1971Phosphoserine; by PKB/AKT2By similarity
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei293 – 2931PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei317 – 3171PhosphothreonineBy similarity
Modified residuei601 – 6011PhosphothreonineBy similarity
Modified residuei643 – 6431PhosphoserineBy similarity
Modified residuei659 – 6591PhosphoserineBy similarity
Modified residuei869 – 8691PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Ser-197 by active myristoylated kinase AKT2; insulin-stimulated phosphorylation by AKT2 regulates SLC2A4/GLUT4 translocation into the plasma membrane.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7TPS5.
PaxDbiQ7TPS5.
PRIDEiQ7TPS5.

PTM databases

PhosphoSiteiQ7TPS5.

Expressioni

Tissue specificityi

Expressed in liver, muscle and fat.

Gene expression databases

BgeeiQ7TPS5.
CleanExiMM_5730419I09RIK.
ExpressionAtlasiQ7TPS5. baseline and differential.
GenevestigatoriQ7TPS5.

Structurei

3D structure databases

ProteinModelPortaliQ7TPS5.
SMRiQ7TPS5. Positions 3-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9090C2PROSITE-ProRule annotationAdd
BLAST

Domaini

The C2 domain binds to calcium and membrane lipids.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG235531.
GeneTreeiENSGT00390000000212.
HOVERGENiHBG081824.
InParanoidiQ7TPS5.
OMAiCQPSCTG.
OrthoDBiEOG715Q38.
PhylomeDBiQ7TPS5.
TreeFamiTF323431.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7TPS5) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ
60 70 80 90 100
WNSEWFKFEV DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE
110 120 130 140 150
AATVISGWFP IYDTIHGIRG EINVVVKVDL FNDSNRFRQS SCGVKFFCTT
160 170 180 190 200
SIPKCYRAVV IHGFVEELVV NEDPEYQWID RIRTPRASNE ARQRLISLMS
210 220 230 240 250
GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG TACTLDKLSS
260 270 280 290 300
PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY
310 320 330 340 350
SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF
360 370 380 390 400
LTLTAFPPGL LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK
410 420 430 440 450
SHAKALGCHA VVGYSESTSI CEEVCILSAS GTAAVLNPRF LQEGTVEGCL
460 470 480 490 500
EQRIEENLPV GCGFCHIPYD ELNMPFPAHL TYCYNCRKQK VPDVLFTTID
510 520 530 540 550
LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF MEYEVHTQLM
560 570 580 590 600
NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK
610 620 630 640 650
TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ
660 670 680 690 700
RSRLLRSQSE SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP
710 720 730 740 750
PSGFYSCNTE IMPGINNWTS EIQMFTSVRV VRLSSLNLTN QALNKNFNGL
760 770 780 790 800
CENLLKSLYF KLRSMTPCCL CHVNFTVSLP EDELIQVTVT AVAITFDKNQ
810 820 830 840 850
ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF PAAKEHLESA
860 870 880 890 900
NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI
910 920 930 940 950
PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA
960 970 980 990 1000
LGGNAVVSYI MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ
1010
QPAANCQPSC TGEVTT
Length:1,016
Mass (Da):111,664
Last modified:July 25, 2006 - v2
Checksum:iA1E3614203DFCD03
GO
Isoform 2 (identifier: Q7TPS5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-316: IPFNEDPNPN...SDTDLSLTPK → SPLVHPPSHG...FSVSVPTLIY
     845-861: Missing.

Show »
Length:990
Mass (Da):108,869
Checksum:iBEE3F6FFFB05366F
GO
Isoform 3 (identifier: Q7TPS5-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Note: No experimental confirmation available.

Show »
Length:818
Mass (Da):88,974
Checksum:iBD6948023B725703
GO

Sequence cautioni

The sequence BAD32244.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111A → S in BAC26634. (PubMed:16141072)Curated
Sequence conflicti590 – 5901P → T in BAC26634. (PubMed:16141072)Curated
Sequence conflicti632 – 6321T → S in BAD32244. (PubMed:15368895)Curated
Sequence conflicti632 – 6321T → S in AAH27763. (PubMed:15489334)Curated
Sequence conflicti632 – 6321T → S in AAH49905. (PubMed:15489334)Curated
Sequence conflicti793 – 7931A → V in AAH53913. (PubMed:15489334)Curated
Sequence conflicti868 – 8681A → T in BAB30814. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 198198Missing in isoform 3. 1 PublicationVSP_019991Add
BLAST
Alternative sequencei268 – 31649IPFNE…SLTPK → SPLVHPPSHGCRSTHNSPIH TATGSRLTQNFSVSVPTLIY in isoform 2. 3 PublicationsVSP_019992Add
BLAST
Alternative sequencei845 – 86117Missing in isoform 2. 3 PublicationsVSP_019993Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK172966 mRNA. Translation: BAD32244.1. Different initiation.
AK017577 mRNA. Translation: BAB30814.1.
AK029825 mRNA. Translation: BAC26634.1.
AK163233 mRNA. Translation: BAE37246.1.
BC027763 mRNA. Translation: AAH27763.1.
BC049905 mRNA. Translation: AAH49905.1.
BC053913 mRNA. Translation: AAH53913.1.
CCDSiCCDS51952.1. [Q7TPS5-1]
CCDS51953.1. [Q7TPS5-2]
RefSeqiNP_001103158.1. NM_001109688.2. [Q7TPS5-2]
NP_001273507.1. NM_001286578.1. [Q7TPS5-3]
NP_083357.2. NM_029081.3. [Q7TPS5-1]
NP_084173.1. NM_029897.2.
UniGeneiMm.130260.

Genome annotation databases

EnsembliENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. [Q7TPS5-2]
ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279. [Q7TPS5-1]
GeneIDi74741.
KEGGimmu:74741.
UCSCiuc009epx.2. mouse. [Q7TPS5-2]
uc009epy.2. mouse. [Q7TPS5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK172966 mRNA. Translation: BAD32244.1 . Different initiation.
AK017577 mRNA. Translation: BAB30814.1 .
AK029825 mRNA. Translation: BAC26634.1 .
AK163233 mRNA. Translation: BAE37246.1 .
BC027763 mRNA. Translation: AAH27763.1 .
BC049905 mRNA. Translation: AAH49905.1 .
BC053913 mRNA. Translation: AAH53913.1 .
CCDSi CCDS51952.1. [Q7TPS5-1 ]
CCDS51953.1. [Q7TPS5-2 ]
RefSeqi NP_001103158.1. NM_001109688.2. [Q7TPS5-2 ]
NP_001273507.1. NM_001286578.1. [Q7TPS5-3 ]
NP_083357.2. NM_029081.3. [Q7TPS5-1 ]
NP_084173.1. NM_029897.2.
UniGenei Mm.130260.

3D structure databases

ProteinModelPortali Q7TPS5.
SMRi Q7TPS5. Positions 3-125.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q7TPS5.

Proteomic databases

MaxQBi Q7TPS5.
PaxDbi Q7TPS5.
PRIDEi Q7TPS5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087485 ; ENSMUSP00000084758 ; ENSMUSG00000030279 . [Q7TPS5-2 ]
ENSMUST00000111758 ; ENSMUSP00000107388 ; ENSMUSG00000030279 . [Q7TPS5-1 ]
GeneIDi 74741.
KEGGi mmu:74741.
UCSCi uc009epx.2. mouse. [Q7TPS5-2 ]
uc009epy.2. mouse. [Q7TPS5-1 ]

Organism-specific databases

CTDi 9847.
MGIi MGI:1921991. C2cd5.
Rougei Search...

Phylogenomic databases

eggNOGi NOG235531.
GeneTreei ENSGT00390000000212.
HOVERGENi HBG081824.
InParanoidi Q7TPS5.
OMAi CQPSCTG.
OrthoDBi EOG715Q38.
PhylomeDBi Q7TPS5.
TreeFami TF323431.

Miscellaneous databases

NextBioi 341536.
SOURCEi Search...

Gene expression databases

Bgeei Q7TPS5.
CleanExi MM_5730419I09RIK.
ExpressionAtlasi Q7TPS5. baseline and differential.
Genevestigatori Q7TPS5.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR000008. C2_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
PROSITEi PS50004. C2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Fetal brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 710-1016 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Egg, Embryo and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 513-1016 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Egg and Mammary tumor.
  4. "C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane."
    Xie X., Gong Z., Mansuy-Aubert V., Zhou Q.L., Tatulian S.A., Sehrt D., Gnad F., Brill L.M., Motamedchaboki K., Chen Y., Czech M.P., Mann M., Kruger M., Jiang Z.Y.
    Cell Metab. 14:378-389(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiC2CD5_MOUSE
AccessioniPrimary (citable) accession number: Q7TPS5
Secondary accession number(s): Q3TQY6
, Q6A052, Q80VA1, Q8C0U3, Q8CID5, Q9CS85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3