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Q7TPS5

- C2CD5_MOUSE

UniProt

Q7TPS5 - C2CD5_MOUSE

Protein

C2 domain-containing protein 5

Gene

C2cd5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.1 Publication

    Cofactori

    Binds 2 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi13 – 27151; low affinityBy similarityAdd
    BLAST
    Calcium bindingi75 – 86122; high affinityBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent phospholipid binding Source: UniProtKB
    2. calcium ion binding Source: MGI

    GO - Biological processi

    1. cellular response to insulin stimulus Source: UniProtKB
    2. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: UniProtKB
    3. intracellular protein transmembrane transport Source: UniProtKB
    4. positive regulation of glucose import in response to insulin stimulus Source: MGI
    5. positive regulation of glucose transport Source: UniProtKB
    6. positive regulation of protein targeting to membrane Source: UniProtKB
    7. positive regulation of vesicle fusion Source: UniProtKB
    8. protein localization to plasma membrane Source: MGI
    9. vesicle fusion Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Calcium, Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C2 domain-containing protein 5
    Alternative name(s):
    138 kDa C2 domain-containing phosphoprotein
    Gene namesi
    Name:C2cd5
    Synonyms:Cdp138, Kiaa0528
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1921991. C2cd5.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity. Cytoplasmcell cortex By similarity. Cell membrane By similarity. Cell projectionruffle By similarity
    Note: Dynamically associated with GLUT4-containing glucose storage vesicles (GSV) and plasma membrane in response to insulin stimulation.By similarity

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB
    2. cytoplasmic vesicle membrane Source: UniProtKB
    3. plasma membrane Source: MGI
    4. ruffle membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10161016C2 domain-containing protein 5PRO_0000247451Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei197 – 1971Phosphoserine; by PKB/AKT2By similarity
    Modified residuei200 – 2001PhosphoserineBy similarity
    Modified residuei293 – 2931PhosphoserineBy similarity
    Modified residuei295 – 2951PhosphoserineBy similarity
    Modified residuei317 – 3171PhosphothreonineBy similarity
    Modified residuei601 – 6011PhosphothreonineBy similarity
    Modified residuei643 – 6431PhosphoserineBy similarity
    Modified residuei659 – 6591PhosphoserineBy similarity
    Modified residuei869 – 8691PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-197 by active myristoylated kinase AKT2; insulin-stimulated phosphorylation by AKT2 regulates SLC2A4/GLUT4 translocation into the plasma membrane.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ7TPS5.
    PaxDbiQ7TPS5.
    PRIDEiQ7TPS5.

    PTM databases

    PhosphoSiteiQ7TPS5.

    Expressioni

    Tissue specificityi

    Expressed in liver, muscle and fat.

    Gene expression databases

    BgeeiQ7TPS5.
    CleanExiMM_5730419I09RIK.
    GenevestigatoriQ7TPS5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TPS5.
    SMRiQ7TPS5. Positions 3-128.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9090C2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The C2 domain binds to calcium and membrane lipids.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG235531.
    GeneTreeiENSGT00390000000212.
    HOVERGENiHBG081824.
    InParanoidiQ7TPS5.
    OMAiCQPSCTG.
    OrthoDBiEOG715Q38.
    PhylomeDBiQ7TPS5.
    TreeFamiTF323431.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7TPS5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ     50
    WNSEWFKFEV DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE 100
    AATVISGWFP IYDTIHGIRG EINVVVKVDL FNDSNRFRQS SCGVKFFCTT 150
    SIPKCYRAVV IHGFVEELVV NEDPEYQWID RIRTPRASNE ARQRLISLMS 200
    GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG TACTLDKLSS 250
    PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY 300
    SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF 350
    LTLTAFPPGL LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK 400
    SHAKALGCHA VVGYSESTSI CEEVCILSAS GTAAVLNPRF LQEGTVEGCL 450
    EQRIEENLPV GCGFCHIPYD ELNMPFPAHL TYCYNCRKQK VPDVLFTTID 500
    LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF MEYEVHTQLM 550
    NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK 600
    TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ 650
    RSRLLRSQSE SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP 700
    PSGFYSCNTE IMPGINNWTS EIQMFTSVRV VRLSSLNLTN QALNKNFNGL 750
    CENLLKSLYF KLRSMTPCCL CHVNFTVSLP EDELIQVTVT AVAITFDKNQ 800
    ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF PAAKEHLESA 850
    NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI 900
    PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA 950
    LGGNAVVSYI MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ 1000
    QPAANCQPSC TGEVTT 1016
    Length:1,016
    Mass (Da):111,664
    Last modified:July 25, 2006 - v2
    Checksum:iA1E3614203DFCD03
    GO
    Isoform 2 (identifier: Q7TPS5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         268-316: IPFNEDPNPN...SDTDLSLTPK → SPLVHPPSHG...FSVSVPTLIY
         845-861: Missing.

    Show »
    Length:990
    Mass (Da):108,869
    Checksum:iBEE3F6FFFB05366F
    GO
    Isoform 3 (identifier: Q7TPS5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-198: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:818
    Mass (Da):88,974
    Checksum:iBD6948023B725703
    GO

    Sequence cautioni

    The sequence BAD32244.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111A → S in BAC26634. (PubMed:16141072)Curated
    Sequence conflicti590 – 5901P → T in BAC26634. (PubMed:16141072)Curated
    Sequence conflicti632 – 6321T → S in BAD32244. (PubMed:15368895)Curated
    Sequence conflicti632 – 6321T → S in AAH27763. (PubMed:15489334)Curated
    Sequence conflicti632 – 6321T → S in AAH49905. (PubMed:15489334)Curated
    Sequence conflicti793 – 7931A → V in AAH53913. (PubMed:15489334)Curated
    Sequence conflicti868 – 8681A → T in BAB30814. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 198198Missing in isoform 3. 1 PublicationVSP_019991Add
    BLAST
    Alternative sequencei268 – 31649IPFNE…SLTPK → SPLVHPPSHGCRSTHNSPIH TATGSRLTQNFSVSVPTLIY in isoform 2. 3 PublicationsVSP_019992Add
    BLAST
    Alternative sequencei845 – 86117Missing in isoform 2. 3 PublicationsVSP_019993Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK172966 mRNA. Translation: BAD32244.1. Different initiation.
    AK017577 mRNA. Translation: BAB30814.1.
    AK029825 mRNA. Translation: BAC26634.1.
    AK163233 mRNA. Translation: BAE37246.1.
    BC027763 mRNA. Translation: AAH27763.1.
    BC049905 mRNA. Translation: AAH49905.1.
    BC053913 mRNA. Translation: AAH53913.1.
    CCDSiCCDS51952.1. [Q7TPS5-1]
    CCDS51953.1. [Q7TPS5-2]
    RefSeqiNP_001103158.1. NM_001109688.2. [Q7TPS5-2]
    NP_001273507.1. NM_001286578.1. [Q7TPS5-3]
    NP_083357.2. NM_029081.3. [Q7TPS5-1]
    NP_084173.1. NM_029897.2.
    UniGeneiMm.130260.

    Genome annotation databases

    EnsembliENSMUST00000087485; ENSMUSP00000084758; ENSMUSG00000030279. [Q7TPS5-2]
    ENSMUST00000111758; ENSMUSP00000107388; ENSMUSG00000030279. [Q7TPS5-1]
    GeneIDi74741.
    KEGGimmu:74741.
    UCSCiuc009epx.2. mouse. [Q7TPS5-2]
    uc009epy.2. mouse. [Q7TPS5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK172966 mRNA. Translation: BAD32244.1 . Different initiation.
    AK017577 mRNA. Translation: BAB30814.1 .
    AK029825 mRNA. Translation: BAC26634.1 .
    AK163233 mRNA. Translation: BAE37246.1 .
    BC027763 mRNA. Translation: AAH27763.1 .
    BC049905 mRNA. Translation: AAH49905.1 .
    BC053913 mRNA. Translation: AAH53913.1 .
    CCDSi CCDS51952.1. [Q7TPS5-1 ]
    CCDS51953.1. [Q7TPS5-2 ]
    RefSeqi NP_001103158.1. NM_001109688.2. [Q7TPS5-2 ]
    NP_001273507.1. NM_001286578.1. [Q7TPS5-3 ]
    NP_083357.2. NM_029081.3. [Q7TPS5-1 ]
    NP_084173.1. NM_029897.2.
    UniGenei Mm.130260.

    3D structure databases

    ProteinModelPortali Q7TPS5.
    SMRi Q7TPS5. Positions 3-128.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q7TPS5.

    Proteomic databases

    MaxQBi Q7TPS5.
    PaxDbi Q7TPS5.
    PRIDEi Q7TPS5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000087485 ; ENSMUSP00000084758 ; ENSMUSG00000030279 . [Q7TPS5-2 ]
    ENSMUST00000111758 ; ENSMUSP00000107388 ; ENSMUSG00000030279 . [Q7TPS5-1 ]
    GeneIDi 74741.
    KEGGi mmu:74741.
    UCSCi uc009epx.2. mouse. [Q7TPS5-2 ]
    uc009epy.2. mouse. [Q7TPS5-1 ]

    Organism-specific databases

    CTDi 9847.
    MGIi MGI:1921991. C2cd5.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG235531.
    GeneTreei ENSGT00390000000212.
    HOVERGENi HBG081824.
    InParanoidi Q7TPS5.
    OMAi CQPSCTG.
    OrthoDBi EOG715Q38.
    PhylomeDBi Q7TPS5.
    TreeFami TF323431.

    Miscellaneous databases

    NextBioi 341536.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q7TPS5.
    CleanExi MM_5730419I09RIK.
    Genevestigatori Q7TPS5.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Fetal brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 710-1016 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Egg, Embryo and Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 513-1016 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Egg and Mammary tumor.
    4. "C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane."
      Xie X., Gong Z., Mansuy-Aubert V., Zhou Q.L., Tatulian S.A., Sehrt D., Gnad F., Brill L.M., Motamedchaboki K., Chen Y., Czech M.P., Mann M., Kruger M., Jiang Z.Y.
      Cell Metab. 14:378-389(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiC2CD5_MOUSE
    AccessioniPrimary (citable) accession number: Q7TPS5
    Secondary accession number(s): Q3TQY6
    , Q6A052, Q80VA1, Q8C0U3, Q8CID5, Q9CS85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3