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Protein

Ribosomal protein S6 kinase alpha-6

Gene

Rps6ka6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Constitutively activated by phosphorylation at Ser-252, Ser-392, and Ser-409 in serum-starved cells. Does not require growth factor stimulation for significant kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125ATPPROSITE-ProRule annotation1
Active sitei218Proton acceptorBy similarity1
Binding sitei475ATPPROSITE-ProRule annotation1
Active sitei563Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 107ATPPROSITE-ProRule annotation9
Nucleotide bindingi452 – 460ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S6 kinase alpha-6 (EC:2.7.11.1)
Short name:
S6K-alpha-6
Gene namesi
Name:Rps6ka6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1914321. Rps6ka6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781621 – 764Ribosomal protein S6 kinase alpha-6Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphoserineBy similarity1
Modified residuei392PhosphoserineBy similarity1
Modified residuei409PhosphoserineCombined sources1
Modified residuei601PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-252, Ser-392, and Ser-409 in serum-starved cells.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TPS0.
PRIDEiQ7TPS0.

PTM databases

iPTMnetiQ7TPS0.
PhosphoSitePlusiQ7TPS0.

Expressioni

Gene expression databases

CleanExiMM_RPS6KA6.

Interactioni

Subunit structurei

Forms a complex with MAPK3/ERK1 but not with MAPK9 or MAPK14 in serum-starved cells.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080694.

Structurei

3D structure databases

ProteinModelPortaliQ7TPS0.
SMRiQ7TPS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 350Protein kinase 1PROSITE-ProRule annotationAdd BLAST258
Domaini351 – 420AGC-kinase C-terminalAdd BLAST70
Domaini446 – 706Protein kinase 2PROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 protein kinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ7TPS0.
KOiK04373.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016239. Ribosomal_S6_kinase_II.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000606. Ribsml_S6_kin_2. 1 hit.
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 2 hits.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00108. PROTEIN_KINASE_ST. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TPS0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPFAPVEDP WDQEDMEVFG STSSSEPQVV FTMKNAATVM REHERKEVND
60 70 80 90 100
LKMVDEPMEE GEPVSCRREE LVKEVPITQH VKEGYEKADP AQFDLLKVLG
110 120 130 140 150
QGSFGKVFLV RKKTGPDAGQ LYAMKVLRKA SLKVRDRVRT KMERDILVEV
160 170 180 190 200
NHPFIVKLHY AFQTEGKLYL ILDFLRGGDV FTRLSKEVLF TEEDVKFYLA
210 220 230 240 250
ELALALDHLH RLGIVYRDLK PENILLDEIG HIKLTDFGLS KESVDQEKKA
260 270 280 290 300
YSFCGTVEYM APEVVNRRAH SQSADWWSYG VLMFEMLTGT LPFQGKDRNE
310 320 330 340 350
TMNMILKAKL GMPQFLSAEA QSLLRMLFKR NPANRLGSEG VEEVKRHAFF
360 370 380 390 400
ASIDWNKLYK REVQPPFRPA SGKPDDTFCF DPEFTAKTPK DSPGLPASAN
410 420 430 440 450
AHQLFKGFSF VATSIAEEYK ITPVTSSNVL PIVQINGNAA QFSEAYELKE
460 470 480 490 500
DIGIGSYSVC KRCIHSASNV EFAVKIIDKN KRDPSEEIEI LMRYGQHPNI
510 520 530 540 550
ISLKEVFDDG KYVYLVTDLM KGGELLDRIL KKKCFSEQEA SNVLYVITKT
560 570 580 590 600
VECLHSQGVV HRDLKPSNIL YMDESAHPDS IKICDFGFAK QLRGENGLLL
610 620 630 640 650
TPCYTANFVA PEVLTQQGYD AACDIWSLGV LLYTMLAGYT PFSNGPNDTP
660 670 680 690 700
EEILLRIGNG RFSLSGGIWD NISRGAKDLL SHMLHMDPHQ RYTAEQVLKH
710 720 730 740 750
PWITQREQLP RHQPNSDEPP QEAVAAPYSV LARNPNRHHP ILEPVTASRL
760
AQRRNMKKRT STGL
Length:764
Mass (Da):86,571
Last modified:February 20, 2007 - v2
Checksum:i181053F9E6A70764
GO
Isoform 2 (identifier: Q7TPS0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     678-721: Missing.

Note: No experimental confirmation available.
Show »
Length:720
Mass (Da):81,253
Checksum:i81A6249A801628A9
GO
Isoform 3 (identifier: Q7TPS0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     613-617: Missing.

Note: No experimental confirmation available.
Show »
Length:759
Mass (Da):86,001
Checksum:i8FAD6546501BF7F6
GO

Sequence cautioni

The sequence BAC33698 differs from that shown. Reason: Frameshift at position 714.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75V → I in BAC33698 (PubMed:16141072).Curated1
Sequence conflicti164T → S in BAC33698 (PubMed:16141072).Curated1
Sequence conflicti269A → G in BAC33698 (PubMed:16141072).Curated1
Sequence conflicti450 – 451ED → KR in AK012150 (PubMed:16141072).Curated2
Sequence conflicti632L → M in BAB29568 (PubMed:16141072).Curated1
Sequence conflicti657I → V in BAC33698 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023128613 – 617Missing in isoform 3. 1 Publication5
Alternative sequenceiVSP_023129678 – 721Missing in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012150 mRNA. No translation available.
AK014822 mRNA. Translation: BAB29568.2.
AK049349 mRNA. Translation: BAC33698.1. Sequence problems.
BC054113 mRNA. Translation: AAH54113.1.
RefSeqiNP_080225.2. NM_025949.3.
UniGeneiMm.37617.

Genome annotation databases

GeneIDi67071.
KEGGimmu:67071.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012150 mRNA. No translation available.
AK014822 mRNA. Translation: BAB29568.2.
AK049349 mRNA. Translation: BAC33698.1. Sequence problems.
BC054113 mRNA. Translation: AAH54113.1.
RefSeqiNP_080225.2. NM_025949.3.
UniGeneiMm.37617.

3D structure databases

ProteinModelPortaliQ7TPS0.
SMRiQ7TPS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080694.

PTM databases

iPTMnetiQ7TPS0.
PhosphoSitePlusiQ7TPS0.

Proteomic databases

PaxDbiQ7TPS0.
PRIDEiQ7TPS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi67071.
KEGGimmu:67071.

Organism-specific databases

CTDi27330.
MGIiMGI:1914321. Rps6ka6.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ7TPS0.
KOiK04373.

Miscellaneous databases

ChiTaRSiRps6ka6. mouse.
PROiQ7TPS0.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RPS6KA6.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016239. Ribosomal_S6_kinase_II.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000606. Ribsml_S6_kin_2. 1 hit.
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 2 hits.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00108. PROTEIN_KINASE_ST. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKS6A6_MOUSE
AccessioniPrimary (citable) accession number: Q7TPS0
Secondary accession number(s): Q8BWZ2, Q9CUR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.