Alpha-actinin-1 - Mus musculus (Mouse)

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Q7TPR4 (ACTN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-actinin-1

Alternative name(s):
Alpha-actinin cytoskeletal isoform
F-actin cross-linking protein
Non-muscle alpha-actinin-1

Gene names

Name:

Actn1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.
Subunit structure	Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP By similarity. Interacts with PSD. Ref.3
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasm › myofibril › sarcomere › Z line. Cell membrane. Cell projection › ruffle. Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. Ref.2 Ref.3
Sequence similarities	Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Domain	Repeat
Ligand	Actin-binding Calcium
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin filament bundle assembly Inferred from direct assay. Source: UniProtKB cortical cytoskeleton organization Traceable author statement. Source: UniProtKB
Cellular component	Z disc Inferred from direct assay. Source: MGI dense core granule membrane Traceable author statement. Source: UniProtKB fascia adherens Inferred from direct assay. Source: MGI focal adhesion Traceable author statement. Source: UniProtKB ruffle Inferred from electronic annotation. Source: UniProtKB-SubCell stress fiber Traceable author statement. Source: UniProtKB
Molecular function	actin filament binding Inferred from direct assay. Source: MGI calcium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 892

892

Alpha-actinin-1

PRO_0000073432

Regions

Domain

1 – 247

247

Actin-binding

Domain

31 – 135

105

CH 1

Domain

144 – 247

104

CH 2

Repeat

274 – 384

111

Spectrin 1

Repeat

394 – 499

106

Spectrin 2

Repeat

509 – 620

112

Spectrin 3

Repeat

630 – 733

104

Spectrin 4

Domain

746 – 781

EF-hand 1

Domain

787 – 822

EF-hand 2

Calcium binding

759 – 770

1 Potential

Calcium binding

800 – 811

2 Potential

Region

274 – 733

460

Interaction with DDN By similarity

Amino acid modifications

Modified residue

Phosphotyrosine; by FAK1 By similarity

Modified residue

Phosphothreonine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

140

Phosphoserine By similarity

Modified residue

193

Phosphotyrosine By similarity

Modified residue

195

N6-acetyllysine By similarity

Modified residue

246

Phosphotyrosine By similarity

Modified residue

676

N6-acetyllysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q7TPR4 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 652DA065A5F38E7C
Show »

FASTA

892

103,068

        10         20         30         40         50         60 
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR 

        70         80         90        100        110        120 
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV 

       130        140        150        160        170        180 
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC 

       190        200        210        220        230        240 
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT 

       250        260        270        280        290        300 
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR 

       310        320        330        340        350        360 
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM 

       370        380        390        400        410        420 
VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK 

       430        440        450        460        470        480 
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC 

       490        500        510        520        530        540 
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT 

       550        560        570        580        590        600 
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI 

       610        620        630        640        650        660 
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM 

       670        680        690        700        710        720 
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI FDNKHTNYNM EHIRVGWEQL 

       730        740        750        760        770        780 
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY 

       790        800        810        820        830        840 
DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK 

       850        860        870        880        890 
NYITEDELRR ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[2]	"Calsarcins, a novel family of sarcomeric calcineurin-binding proteins." Frey N., Richardson J.A., Olson E.N. Proc. Natl. Acad. Sci. U.S.A. 97:14632-14637(2000) [PubMed: 11114196] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines." Sakagami H., Honma T., Sukegawa J., Owada Y., Yanagisawa T., Kondo H. Eur. J. Neurosci. 25:618-628(2007) [PubMed: 17298598] [Abstract] Cited for: INTERACTION WITH PSD, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC054830 mRNA. Translation: AAH54830.1.
IPI	IPI00380436.
RefSeq	NP_598917.1. NM_134156.2.
UniGene	Mm.253564. Mm.403477.
3D structure databases
ProteinModelPortal	Q7TPR4.
SMR	Q7TPR4. Positions 30-254, 267-892.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TPR4. 3 interactions.
STRING	Q7TPR4.
PTM databases
PhosphoSite	Q7TPR4.
Proteomic databases
PRIDE	Q7TPR4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143.
GeneID	109711.
KEGG	mmu:109711.
UCSC	uc007oap.2. mouse.
Organism-specific databases
CTD	87.
MGI	MGI:2137706. Actn1.
Phylogenomic databases
HOGENOM	HBG314462.
HOVERGEN	HBG050453.
InParanoid	Q7TPR4.
OMA	IGNDPQK.
OrthoDB	EOG49P9XR.
PhylomeDB	Q7TPR4.
Gene expression databases
ArrayExpress	Q7TPR4.
Bgee	Q7TPR4.
CleanEx	MM_ACTN1.
Genevestigator	Q7TPR4.
GermOnline	ENSMUSG00000015143. Mus musculus.
Family and domain databases
InterPro	IPR001589. Actinin_actin-bd_CS. IPR001715. CH-domain. IPR011992. EF-hand-like_dom. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 2 hits. G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
KO	K05699.
Pfam	PF00307. CH. 2 hits. PF08726. efhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits. [Graphical view]
SMART	SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 2 hits. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	362623.
SOURCE	Search...

Entry information

Entry name

ACTN1_MOUSE

Accession

Primary (citable) accession number: Q7TPR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents