Q7TPR4 (ACTN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-actinin-1

Alternative name(s):
Alpha-actinin cytoskeletal isoform
F-actin cross-linking protein
Non-muscle alpha-actinin-1

Gene names

Name:

Actn1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.
Subunit structure	Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP By similarity. Interacts with PSD. Ref.3
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasm › myofibril › sarcomere › Z line. Cell membrane. Cell projection › ruffle. Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. Ref.2 Ref.3
Sequence similarities	Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Domain	Repeat
Ligand	Actin-binding Calcium Metal-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin crosslink formation Inferred from electronic annotation. Source: InterPro actin filament bundle assembly Inferred from direct assay PubMed 16043482. Source: UniProtKB cortical cytoskeleton organization Traceable author statement PubMed 15841212. Source: UniProtKB focal adhesion assembly Inferred from electronic annotation. Source: Compara negative regulation of cellular component movement Inferred from electronic annotation. Source: Compara
Cellular_component	Z disc Inferred from direct assay PubMed 15665106PubMed 18332105PubMed 20215401PubMed 20368620. Source: MGI cortical cytoskeleton Traceable author statement PubMed 15841212. Source: UniProtKB dendritic spine Inferred from electronic annotation. Source: Compara dense core granule membrane Traceable author statement PubMed 16043482. Source: UniProtKB fascia adherens Inferred from direct assay PubMed 11732910. Source: MGI focal adhesion Traceable author statement PubMed 15841212. Source: UniProtKB nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ruffle Inferred from electronic annotation. Source: UniProtKB-SubCell stress fiber Traceable author statement PubMed 15841212. Source: UniProtKB
Molecular_function	actin filament binding Inferred from direct assay PubMed 15465019. Source: MGI calcium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Traceable author statement PubMed 15841212. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alpha-actinin-1		PRO_0000073432
Regions
	Domain	1 – 247	247	Actin-binding
	Domain	31 – 135	105	CH 1
	Domain	144 – 247	104	CH 2
	Repeat	274 – 384	111	Spectrin 1
	Repeat	394 – 499	106	Spectrin 2
	Repeat	509 – 620	112	Spectrin 3
	Repeat	630 – 733	104	Spectrin 4
	Domain	746 – 781	36	EF-hand 1
	Domain	787 – 822	36	EF-hand 2
	Calcium binding	759 – 770	12	1 Potential
	Calcium binding	800 – 811	12	2 Potential
	Region	274 – 733	460	Interaction with DDN By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	12	1	Phosphotyrosine; by FAK1 By similarity
	Modified residue	95	1	N6-acetyllysine By similarity
	Modified residue	195	1	N6-acetyllysine By similarity
	Modified residue	676	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TPR4 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 652DA065A5F38E7C	FASTA	892	103,068
	10 20 30 40 50 60 MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR 70 80 90 100 110 120 DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV 130 140 150 160 170 180 KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC 190 200 210 220 230 240 ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT 250 260 270 280 290 300 YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR 310 320 330 340 350 360 VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM 370 380 390 400 410 420 VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK 430 440 450 460 470 480 DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC 490 500 510 520 530 540 DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT 550 560 570 580 590 600 IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI 610 620 630 640 650 660 NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM 670 680 690 700 710 720 HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI FDNKHTNYNM EHIRVGWEQL 730 740 750 760 770 780 LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY 790 800 810 820 830 840 DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK 850 860 870 880 890 NYITEDELRR ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[2]	"Calsarcins, a novel family of sarcomeric calcineurin-binding proteins." Frey N., Richardson J.A., Olson E.N. Proc. Natl. Acad. Sci. U.S.A. 97:14632-14637(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines." Sakagami H., Honma T., Sukegawa J., Owada Y., Yanagisawa T., Kondo H. Eur. J. Neurosci. 25:618-628(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PSD, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC054830 mRNA. Translation: AAH54830.1.
IPI	IPI00380436.
RefSeq	NP_598917.1. NM_134156.2.
UniGene	Mm.253564. Mm.403477.
3D structure databases
ProteinModelPortal	Q7TPR4.
SMR	Q7TPR4. Positions 30-254, 267-892.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7TPR4. 3 interactions.
STRING	10090.ENSMUSP00000021554.
PTM databases
PhosphoSite	Q7TPR4.
Proteomic databases
PaxDb	Q7TPR4.
PRIDE	Q7TPR4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143.
GeneID	109711.
KEGG	mmu:109711.
UCSC	uc007oap.2. mouse.
Organism-specific databases
CTD	87.
MGI	MGI:2137706. Actn1.
Phylogenomic databases
eggNOG	COG5069.
GeneTree	ENSGT00670000097825.
HOGENOM	HOG000263418.
HOVERGEN	HBG050453.
InParanoid	Q7TPR4.
KO	K05699.
OMA	MLQSQDF.
OrthoDB	EOG49P9XR.
Gene expression databases
ArrayExpress	Q7TPR4.
Bgee	Q7TPR4.
CleanEx	MM_ACTN1.
Genevestigator	Q7TPR4.
GermOnline	ENSMUSG00000015143. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 2 hits. 1.10.418.10. 2 hits.
InterPro	IPR001589. Actinin_actin-bd_CS. IPR026921. Alpha-actinin. IPR001715. CH-domain. IPR011992. EF-hand-like_dom. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat. [Graphical view]
PANTHER	PTHR11915:SF47. PTHR11915:SF47. 1 hit.
Pfam	PF00307. CH. 2 hits. PF13405. EF_hand_4. 1 hit. PF08726. efhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits. [Graphical view]
SMART	SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 2 hits. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ACTN1. mouse.
NextBio	362623.
SOURCE	Search...

Entry information

Entry name

ACTN1_MOUSE

Accession

Primary (citable) accession number: Q7TPR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program