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Protein

Alpha-actinin-1

Gene

Actn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).HMP:

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi759 – 7701IEP:Add BLAST12
Calcium bindingi800 – 8112IEP:Add BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-446388. Regulation of cytoskeletal remodeling and cell spreading by IPP complex components.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-1
Alternative name(s):
Alpha-actinin cytoskeletal isoform
F-actin cross-linking protein
Non-muscle alpha-actinin-1
Gene namesi
Name:Actn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2137706. Actn1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32Q → K: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication1
Mutagenesisi105V → I: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000734321 – 892Alpha-actinin-1Add BLAST892

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineHMP:1
Modified residuei6PhosphoserineHMP:1
Modified residuei12Phosphotyrosine; by FAK1HMP:1
Modified residuei95N6-acetyllysineHMP:1
Modified residuei195N6-acetyllysineHMP:1
Modified residuei471PhosphoserineHMP:1
Modified residuei676N6-acetyllysineHMP:1
Modified residuei677PhosphoserineHMP:1
Modified residuei890PhosphoserineHMP:1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7TPR4.
MaxQBiQ7TPR4.
PaxDbiQ7TPR4.
PeptideAtlasiQ7TPR4.
PRIDEiQ7TPR4.

PTM databases

iPTMnetiQ7TPR4.
PhosphoSitePlusiQ7TPR4.
SwissPalmiQ7TPR4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000015143.
CleanExiMM_ACTN1.
ExpressionAtlasiQ7TPR4. baseline and differential.
GenevisibleiQ7TPR4. MM.

Interactioni

Subunit structurei

Homodimer; antiparallel. Interacts with MYOZ2, TTID and LPP. Interacts with DDN (By similarity). Interacts with PSD (PubMed:17298598). Interacts with MICALL2 (PubMed:23100251). Interacts with DNM2 and CTTN. Interacts with PDLIM1. Interacts with PDLIM2. Interacts with PDLIM4 (via PDZ domain) (By similarity).HMP:2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224977. 16 interactors.
CORUMiQ7TPR4.
IntActiQ7TPR4. 10 interactors.
MINTiMINT-1869732.
STRINGi10090.ENSMUSP00000021554.

Structurei

3D structure databases

ProteinModelPortaliQ7TPR4.
SMRiQ7TPR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 135Calponin-homology (CH) 1IEP:Add BLAST105
Domaini144 – 250Calponin-homology (CH) 2IEP:Add BLAST107
Repeati274 – 384Spectrin 1Add BLAST111
Repeati394 – 499Spectrin 2Add BLAST106
Repeati509 – 620Spectrin 3Add BLAST112
Repeati630 – 733Spectrin 4Add BLAST104
Domaini746 – 781EF-hand 1IEP:Add BLAST36
Domaini787 – 822EF-hand 2IEP:Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 247Actin-bindingAdd BLAST247
Regioni274 – 733Interaction with DDNHMP:Add BLAST460

Sequence similaritiesi

Belongs to the alpha-actinin family.IKR:

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118813.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiQ7TPR4.
KOiK05699.
OMAiWLNTKLE.
OrthoDBiEOG091G020R.
PhylomeDBiQ7TPR4.
TreeFamiTF352676.

Family and domain databases

CDDicd00014. CH. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.418.10. 2 hits.
InterProiView protein in InterPro
IPR001589. Actinin_actin-bd_CS.
IPR026921. Alpha-actinin_1.
IPR036872. Calponin-like_dom_sf.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
PANTHERiPTHR43947:SF3. PTHR43947:SF3. 1 hit.
PfamiView protein in Pfam
PF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
SMARTiView protein in SMART
SM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiView protein in PROSITE
PS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q7TPR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT
60 70 80 90 100
QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI
110 120 130 140 150
ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL
160 170 180 190 200
LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD
210 220 230 240 250
PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS
260 270 280 290 300
GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
310 320 330 340 350
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR
360 370 380 390 400
PAFMPSEGRM VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR
410 420 430 440 450
QKASIHEAWT DGKEAMLRQK DYETATLSEI KALLKKHEAF ESDLAAHQDR
460 470 480 490 500
VEQIAAIAQE LNELDYYDSP SVNARCQKIC DQWDNLGALT QKRREALERT
510 520 530 540 550
EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT IEEIQGLTTA
560 570 580 590 600
HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
610 620 630 640 650
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM
660 670 680 690 700
EEIGRISIEM HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI
710 720 730 740 750
FDNKHTNYNM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMNE
760 770 780 790 800
FRASFNHFDR DHSGTLGPEE FKACLISLGY DIGNDPQGEA EFARIMSIVD
810 820 830 840 850
PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK NYITEDELRR
860 870 880 890
ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL
Length:892
Mass (Da):103,068
Last modified:October 1, 2003 - v1
Checksum:i652DA065A5F38E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC054830 mRNA. Translation: AAH54830.1.
CCDSiCCDS26011.1.
RefSeqiNP_598917.1. NM_134156.2.
UniGeneiMm.253564.
Mm.403477.

Genome annotation databases

EnsembliENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143.
GeneIDi109711.
KEGGimmu:109711.
UCSCiuc007oap.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiACTN1_MOUSE
AccessioniPrimary (citable) accession number: Q7TPR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2003
Last modified: November 22, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families