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Q7TPR4

- ACTN1_MOUSE

UniProt

Q7TPR4 - ACTN1_MOUSE

Protein

Alpha-actinin-1

Gene

Actn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi759 – 770121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi800 – 811122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. actin filament binding Source: MGI
    2. calcium ion binding Source: InterPro
    3. double-stranded RNA binding Source: MGI
    4. protein binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. actin crosslink formation Source: InterPro
    2. actin filament bundle assembly Source: UniProtKB
    3. cortical cytoskeleton organization Source: UniProtKB
    4. focal adhesion assembly Source: Ensembl
    5. negative regulation of cellular component movement Source: Ensembl

    Keywords - Ligandi

    Actin-binding, Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196644. Syndecan interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-actinin-1
    Alternative name(s):
    Alpha-actinin cytoskeletal isoform
    F-actin cross-linking protein
    Non-muscle alpha-actinin-1
    Gene namesi
    Name:Actn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2137706. Actn1.

    Subcellular locationi

    Cytoplasmcytoskeleton. CytoplasmmyofibrilsarcomereZ line. Cell membrane. Cell junction. Cell projectionruffle
    Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures.

    GO - Cellular componenti

    1. cortical cytoskeleton Source: UniProtKB
    2. dendritic spine Source: Ensembl
    3. dense core granule membrane Source: UniProtKB
    4. extracellular vesicular exosome Source: Ensembl
    5. fascia adherens Source: MGI
    6. focal adhesion Source: UniProtKB
    7. nucleus Source: Ensembl
    8. plasma membrane Source: UniProtKB-SubCell
    9. ruffle Source: UniProtKB-SubCell
    10. secretory granule Source: UniProtKB
    11. stress fiber Source: UniProtKB
    12. Z disc Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi32 – 321Q → K: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication
    Mutagenesisi105 – 1051V → I: Shows less organization of the circumferential actin-filament network compared to controls. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Alpha-actinin-1PRO_0000073432Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei12 – 121Phosphotyrosine; by FAK1By similarity
    Modified residuei95 – 951N6-acetyllysineBy similarity
    Modified residuei195 – 1951N6-acetyllysineBy similarity
    Modified residuei676 – 6761N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ7TPR4.
    PaxDbiQ7TPR4.
    PRIDEiQ7TPR4.

    PTM databases

    PhosphoSiteiQ7TPR4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ7TPR4.
    BgeeiQ7TPR4.
    CleanExiMM_ACTN1.
    GenevestigatoriQ7TPR4.

    Interactioni

    Subunit structurei

    Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP By similarity. Interacts with PSD. Interacts with MICALL2.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi224977. 13 interactions.
    IntActiQ7TPR4. 7 interactions.
    MINTiMINT-1869732.
    STRINGi10090.ENSMUSP00000021554.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TPR4.
    SMRiQ7TPR4. Positions 30-254, 267-892.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 247247Actin-bindingAdd
    BLAST
    Domaini31 – 135105CH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini144 – 247104CH 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati274 – 384111Spectrin 1Add
    BLAST
    Repeati394 – 499106Spectrin 2Add
    BLAST
    Repeati509 – 620112Spectrin 3Add
    BLAST
    Repeati630 – 733104Spectrin 4Add
    BLAST
    Domaini746 – 78136EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini787 – 82236EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni274 – 733460Interaction with DDNBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alpha-actinin family.Curated
    Contains 1 actin-binding domain.Curated
    Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 4 spectrin repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5069.
    GeneTreeiENSGT00670000097825.
    HOGENOMiHOG000263418.
    HOVERGENiHBG050453.
    InParanoidiQ7TPR4.
    KOiK05699.
    OMAiWIRRTMP.
    OrthoDBiEOG72C4ZJ.
    PhylomeDBiQ7TPR4.
    TreeFamiTF352676.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProiIPR001589. Actinin_actin-bd_CS.
    IPR026921. Alpha-actinin_1.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view]
    PANTHERiPTHR11915:SF241. PTHR11915:SF241. 1 hit.
    PfamiPF00307. CH. 2 hits.
    PF13405. EF-hand_6. 1 hit.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view]
    SMARTiSM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 2 hits.
    [Graphical view]
    SUPFAMiSSF47576. SSF47576. 1 hit.
    PROSITEiPS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7TPR4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT    50
    QIENIEEDFR DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI 100
    ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS VEETSAKEGL 150
    LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC ALIHRHRPEL IDYGKLRKDD 200
    PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT YVSSFYHAFS 250
    GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR 300
    VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR 350
    PAFMPSEGRM VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR 400
    QKASIHEAWT DGKEAMLRQK DYETATLSEI KALLKKHEAF ESDLAAHQDR 450
    VEQIAAIAQE LNELDYYDSP SVNARCQKIC DQWDNLGALT QKRREALERT 500
    EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT IEEIQGLTTA 550
    HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI 600
    NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM 650
    EEIGRISIEM HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI 700
    FDNKHTNYNM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMNE 750
    FRASFNHFDR DHSGTLGPEE FKACLISLGY DIGNDPQGEA EFARIMSIVD 800
    PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK NYITEDELRR 850
    ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL 892
    Length:892
    Mass (Da):103,068
    Last modified:October 1, 2003 - v1
    Checksum:i652DA065A5F38E7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC054830 mRNA. Translation: AAH54830.1.
    CCDSiCCDS26011.1.
    RefSeqiNP_598917.1. NM_134156.2.
    UniGeneiMm.253564.
    Mm.403477.

    Genome annotation databases

    EnsembliENSMUST00000021554; ENSMUSP00000021554; ENSMUSG00000015143.
    GeneIDi109711.
    KEGGimmu:109711.
    UCSCiuc007oap.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC054830 mRNA. Translation: AAH54830.1 .
    CCDSi CCDS26011.1.
    RefSeqi NP_598917.1. NM_134156.2.
    UniGenei Mm.253564.
    Mm.403477.

    3D structure databases

    ProteinModelPortali Q7TPR4.
    SMRi Q7TPR4. Positions 30-254, 267-892.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 224977. 13 interactions.
    IntActi Q7TPR4. 7 interactions.
    MINTi MINT-1869732.
    STRINGi 10090.ENSMUSP00000021554.

    PTM databases

    PhosphoSitei Q7TPR4.

    Proteomic databases

    MaxQBi Q7TPR4.
    PaxDbi Q7TPR4.
    PRIDEi Q7TPR4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021554 ; ENSMUSP00000021554 ; ENSMUSG00000015143 .
    GeneIDi 109711.
    KEGGi mmu:109711.
    UCSCi uc007oap.2. mouse.

    Organism-specific databases

    CTDi 87.
    MGIi MGI:2137706. Actn1.

    Phylogenomic databases

    eggNOGi COG5069.
    GeneTreei ENSGT00670000097825.
    HOGENOMi HOG000263418.
    HOVERGENi HBG050453.
    InParanoidi Q7TPR4.
    KOi K05699.
    OMAi WIRRTMP.
    OrthoDBi EOG72C4ZJ.
    PhylomeDBi Q7TPR4.
    TreeFami TF352676.

    Enzyme and pathway databases

    Reactomei REACT_196644. Syndecan interactions.

    Miscellaneous databases

    ChiTaRSi ACTN1. mouse.
    NextBioi 362623.
    PROi Q7TPR4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q7TPR4.
    Bgeei Q7TPR4.
    CleanExi MM_ACTN1.
    Genevestigatori Q7TPR4.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    1.10.418.10. 2 hits.
    InterProi IPR001589. Actinin_actin-bd_CS.
    IPR026921. Alpha-actinin_1.
    IPR001715. CH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR014837. EF-hand_Ca_insen.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR018159. Spectrin/alpha-actinin.
    IPR002017. Spectrin_repeat.
    [Graphical view ]
    PANTHERi PTHR11915:SF241. PTHR11915:SF241. 1 hit.
    Pfami PF00307. CH. 2 hits.
    PF13405. EF-hand_6. 1 hit.
    PF08726. EFhand_Ca_insen. 1 hit.
    PF00435. Spectrin. 4 hits.
    [Graphical view ]
    SMARTi SM00033. CH. 2 hits.
    SM00054. EFh. 2 hits.
    SM00150. SPEC. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47576. SSF47576. 1 hit.
    PROSITEi PS00019. ACTININ_1. 1 hit.
    PS00020. ACTININ_2. 1 hit.
    PS50021. CH. 2 hits.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.
    2. "Calsarcins, a novel family of sarcomeric calcineurin-binding proteins."
      Frey N., Richardson J.A., Olson E.N.
      Proc. Natl. Acad. Sci. U.S.A. 97:14632-14637(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines."
      Sakagami H., Honma T., Sukegawa J., Owada Y., Yanagisawa T., Kondo H.
      Eur. J. Neurosci. 25:618-628(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PSD, SUBCELLULAR LOCATION.
    4. "Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development."
      Sakane A., Abdallah A.A., Nakano K., Honda K., Ikeda W., Nishikawa Y., Matsumoto M., Matsushita N., Kitamura T., Sasaki T.
      J. Biol. Chem. 287:42455-42468(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MICALL2, SUBCELLULAR LOCATION.
    5. Cited for: MUTAGENESIS OF GLN-32 AND VAL-105.

    Entry informationi

    Entry nameiACTN1_MOUSE
    AccessioniPrimary (citable) accession number: Q7TPR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3