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Protein

Arrestin domain-containing protein 3

Gene

Arrdc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed:21982743). Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • fat pad development Source: MGI
  • negative regulation of heat generation Source: MGI
  • negative regulation of locomotion involved in locomotory behavior Source: MGI
  • negative regulation of multicellular organismal metabolic process Source: MGI
  • positive regulation of adrenergic receptor signaling pathway Source: MGI
  • positive regulation of ubiquitin-protein transferase activity Source: MGI
  • skin development Source: MGI
  • temperature homeostasis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Arrestin domain-containing protein 3
Gene namesi
Name:Arrdc3
Synonyms:Kiaa1376
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2145242. Arrdc3.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Lysosome By similarity
  • Endosome By similarity
  • Early endosome By similarity

  • Note: Associated with plasma membrane, as well as with endosomes and lysosomes during endocytosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate, but there is important perinatal lethality and the majority do not survive till weaning. Mutant mice have considerably less body mass and accumulate less body fat than wild-type, in spite of normal food intake. Heterozygous mice display higher oxygen uptake, increased activity levels and higher energy expenditure than wild-type. In addition, they display increased expression of genes required for thermogenesis, increased activation of signaling cascades downstream of beta-adrenergic receptors, more rapid readjustment of body temperature when exposed to cold and increased heat production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Arrestin domain-containing protein 3PRO_0000244350Add
BLAST

Proteomic databases

PaxDbiQ7TPQ9.
PRIDEiQ7TPQ9.

PTM databases

iPTMnetiQ7TPQ9.

Expressioni

Tissue specificityi

Detected in visceral fat, subcutaneous fat, brown fat and skeletal muscle, and at lower levels in kidney.1 Publication

Inductioni

Up-regulated by fasting. Transiently up-regulated during the differentiation of pre-adipocytes.1 Publication

Gene expression databases

BgeeiQ7TPQ9.
CleanExiMM_ARRDC3.
ExpressionAtlasiQ7TPQ9. baseline and differential.
GenevisibleiQ7TPQ9. MM.

Interactioni

Subunit structurei

Interacts (via PPXY motifs) with NEDD4 (via WW domains). Interacts with ADRB2. Interacts with ADRB3. Interacts with HGS (via PPXY motifs). Does not bind TXN (thioredoxin).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096957.

Structurei

3D structure databases

ProteinModelPortaliQ7TPQ9.
SMRiQ7TPQ9. Positions 3-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi346 – 3494PPXY 1Curated
Motifi391 – 3944PPXY 2Curated

Sequence similaritiesi

Belongs to the arrestin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ7TPQ9.
OMAiETWNGKQ.
OrthoDBiEOG7P02HT.
PhylomeDBiQ7TPQ9.
TreeFamiTF313650.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.

Sequencei

Sequence statusi: Complete.

Q7TPQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLGKVKSLT ISFDCLNDSN VPVYSSGDTV SGRVNLEVTG EIRVKSLKIH
60 70 80 90 100
ARGHAKVRWT ESRNAGSNTA YTQNYTEEVE YFNHKDILIG HERDDDNSEE
110 120 130 140 150
GFHTIHSGRH EYAFSFELPQ TPLATSFEGR HGSVRYWVKA ELHRPWLLPV
160 170 180 190 200
KLKKEFTVFE HIDINTPSLL SPQAGTKEKT LCCWFCTSGP ISLSAKIERK
210 220 230 240 250
GYTPGESIQI FAEIENCSSR MVVPKAAIYQ TQAFYAKGKM KEVKQLVANL
260 270 280 290 300
RGESLSSGKT ETWNGKLLKI PPVSPSILDC SIIRVEYSLM VYVDIPGAMD
310 320 330 340 350
LLLSLPLVIG TIPLHPFGSR TSSVSSQCSM SMNWLALALP ERPEAPPSYA
360 370 380 390 400
EVVTEEQRRN NLAPVGACDD FERALQGPLF AYIQEFRFLP PPLYSEIDPN
410
PDQSSEDRPS CPSR
Length:414
Mass (Da):46,275
Last modified:October 1, 2003 - v1
Checksum:iA9F5EA3A2D701F42
GO

Sequence cautioni

The sequence BAC65781.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521V → A in AAH98474 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122499 mRNA. Translation: BAC65781.1. Different initiation.
BC031125 mRNA. Translation: AAH31125.1.
BC054844 mRNA. Translation: AAH54844.1.
BC098474 mRNA. Translation: AAH98474.1.
CCDSiCCDS36736.1.
RefSeqiNP_001036056.1. NM_001042591.1.
UniGeneiMm.423137.

Genome annotation databases

EnsembliENSMUST00000099356; ENSMUSP00000096957; ENSMUSG00000074794.
GeneIDi105171.
KEGGimmu:105171.
UCSCiuc007rhi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122499 mRNA. Translation: BAC65781.1. Different initiation.
BC031125 mRNA. Translation: AAH31125.1.
BC054844 mRNA. Translation: AAH54844.1.
BC098474 mRNA. Translation: AAH98474.1.
CCDSiCCDS36736.1.
RefSeqiNP_001036056.1. NM_001042591.1.
UniGeneiMm.423137.

3D structure databases

ProteinModelPortaliQ7TPQ9.
SMRiQ7TPQ9. Positions 3-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096957.

PTM databases

iPTMnetiQ7TPQ9.

Proteomic databases

PaxDbiQ7TPQ9.
PRIDEiQ7TPQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099356; ENSMUSP00000096957; ENSMUSG00000074794.
GeneIDi105171.
KEGGimmu:105171.
UCSCiuc007rhi.2. mouse.

Organism-specific databases

CTDi57561.
MGIiMGI:2145242. Arrdc3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000237328.
HOVERGENiHBG066469.
InParanoidiQ7TPQ9.
OMAiETWNGKQ.
OrthoDBiEOG7P02HT.
PhylomeDBiQ7TPQ9.
TreeFamiTF313650.

Miscellaneous databases

PROiQ7TPQ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TPQ9.
CleanExiMM_ARRDC3.
ExpressionAtlasiQ7TPQ9. baseline and differential.
GenevisibleiQ7TPQ9. MM.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CD-1 and FVB/N.
    Tissue: Eye, Kidney and Neural stem cell.
  3. Cited for: FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY FASTING, TISSUE SPECIFICITY.

Entry informationi

Entry nameiARRD3_MOUSE
AccessioniPrimary (citable) accession number: Q7TPQ9
Secondary accession number(s): Q4KMN0, Q80TE6, Q8K0L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.