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Q7TPQ3 (SHPRH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase SHPRH

EC=3.6.4.-
EC=6.3.2.-
Alternative name(s):
SNF2, histone-linker, PHD and RING finger domain-containing helicase
Gene names
Name:Shprh
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1674 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63' By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homodimer. Interacts with HLTF, PCNA, UBE2N and RAD18 By similarity.

Tissue specificity

Broadly expressed (at protein level). Ref.1

Domain

The RING finger mediates E3 ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Contains 1 H15 (linker histone H1/H5 globular) domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 PHD-type zinc finger.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence AAO26655.1 differs from that shown. Reason: Probable cloning artifact.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7TPQ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7TPQ3-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     1617-1674: Missing.
Isoform 3 (identifier: Q7TPQ3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1030-1031: EY → RR
     1032-1674: Missing.
Isoform 4 (identifier: Q7TPQ3-5)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     1617-1662: PTIVHRFLIK...EASVLTVAGL → SIRGLECLKTYRYTSTHDHTLSTYLVLLSI
     1663-1674: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16741674E3 ubiquitin-protein ligase SHPRH
PRO_0000284919

Regions

Domain302 – 38483Helicase ATP-binding; first part
Domain433 – 50775H15
Domain701 – 859159Helicase ATP-binding; second part
Domain1505 – 1663159Helicase C-terminal
Nucleotide binding368 – 3758ATP By similarity
Zinc finger649 – 70052PHD-type
Zinc finger1423 – 147048RING-type
Motif810 – 8134DEAQ box

Amino acid modifications

Modified residue2611Phosphoserine By similarity

Natural variations

Alternative sequence1030 – 10312EY → RR in isoform 3.
VSP_024766
Alternative sequence1032 – 1674643Missing in isoform 3.
VSP_024767
Alternative sequence1617 – 167458Missing in isoform 2.
VSP_024768
Alternative sequence1617 – 166246PTIVH…TVAGL → SIRGLECLKTYRYTSTHDHT LSTYLVLLSI in isoform 4.
VSP_024769
Alternative sequence1663 – 167412Missing in isoform 4.
VSP_024770

Experimental info

Sequence conflict1771S → G in BAC38428. Ref.2
Sequence conflict5821Q → H in BAC38428. Ref.2
Sequence conflict9281Q → R in BAC38428. Ref.2
Sequence conflict12851V → I in AAO26654. Ref.1
Sequence conflict12851V → I in AAO26655. Ref.1
Sequence conflict12851V → I in AAO26656. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 2491B2F32F326393

FASTA1,674191,490
        10         20         30         40         50         60 
MSSRRKRAPP MKVDEERQQQ LHWNMHEDLR SEPLTMTVGE QACSDADSSS DCIIIDEGPP 

        70         80         90        100        110        120 
ESALHRDKKR RSETVSVLEA TEEETRLSVT LNVTVSPYRV DNSWKAFLGD FALQLLPKES 

       130        140        150        160        170        180 
LVEHFSERTF TLSPSESSSQ FLIYVHSECK NVEKQENVLE GSAGVCSKGI RVESSFSSDM 

       190        200        210        220        230        240 
LQDLAWLQKR RGIKLYQRPD GTHTIKVGIY ILEAGLTRLD FMSDAGSRMK KFNQLMKRVM 

       250        260        270        280        290        300 
EKLHNFIIPD VLEEEEEGSE SEPEGQDIDE LYHFVKQTHQ QETRSVQVDV QHPALIPVLR 

       310        320        330        340        350        360 
PYQREAVNWM LQQEQFRSAP PADNSLHFLW REIVTPDGLK LYYNPYTGCI IRDFPHAGPQ 

       370        380        390        400        410        420 
LLGGILADEM GLGKTVEVLA LILTHTRQDV KQDALTLPEG KVVNYFIPTH CPREKVKNRE 

       430        440        450        460        470        480 
IQDTEYEPKE KVHCPPTRVM ILTAVKEMNG KKGVSILSIY KYVSSIFRYD VQRNRGLLKR 

       490        500        510        520        530        540 
MLKCLIFEGL VKQIKGHGFS GTFTLGKNYK EDVFDKTKKQ AVGSPRKIEK ELRKSVNKDA 

       550        560        570        580        590        600 
DSEYLPSNTS DDDEPYYYYC KAGKSRSKLK KPALLTKKGK GQSVHLDSQG DAPAAGVCAS 

       610        620        630        640        650        660 
TDVHVSENTC VSEDKQTQEA KDCAESPNPA AEELAQSNTS SPCETSDYRF ECICGEFDQI 

       670        680        690        700        710        720 
GHKPRVQCLK CHLWQHAKCV NYEEKNLKVK PFYCPHCLVA MEPVSTRATL IISPSSICHQ 

       730        740        750        760        770        780 
WVDEINRHVR SSSLRVLVYQ GVKKHGFLQP HFLAEQDIVI ITYDVLRSEL NYVNIPHSNS 

       790        800        810        820        830        840 
EDGRRLRNQK RYMAIPSPLV AVEWWRICLD EAQMVECPTV KAAEMAQRLS GINRWCISGT 

       850        860        870        880        890        900 
PVQRGLEDLF GLVVFLGIEP YCVKHWWIRL LYHPYCKKNP QHLYSFIAKI MWRSAKKDVI 

       910        920        930        940        950        960 
DQIQIPPQTE EMHWLHFSPV ERHFYHRQHE VCCQDAIVKL RKISDWALKL SSLDRRTVSS 

       970        980        990       1000       1010       1020 
ILYPLLRLRQ ACCHPQAVRG EFLPLQKSTM TMEELLTSLQ KKCGTECEEA HRQLVCALNG 

      1030       1040       1050       1060       1070       1080 
LAGIHIIKGE YALAAELYRE VLRSSEEHKG KLKTDSLQRL HATHNLMELL GAKHPGIPPT 

      1090       1100       1110       1120       1130       1140 
LRDGRLEEEA KQLREHYMSK CNTEVAEAQQ ALQPVQQSIR ELQRKIHSNS PWWLNVIHRA 

      1150       1160       1170       1180       1190       1200 
MEFSVDEELV QRVRNEISSN YKQQTDKLSM SEKFRDCRGL QFLLTTQMEE LHKFQKLVRE 

      1210       1220       1230       1240       1250       1260 
AVKKLEKPPS REVIESATVC HLRPARLPLN CCVFCKADEL FTEYESKLFF NTVKGQTAIF 

      1270       1280       1290       1300       1310       1320 
EEMIEDEEGL VDDRVPTTTR GLWAVSETER SMKAILSFAR SHRFDVEYVD EGSVSMDLFE 

      1330       1340       1350       1360       1370       1380 
AWKKEYKLLH EYWMTLRNRV SAVDELAMAT ERLRVRHPKE PKPNPPVHHI IEPHEVEQNR 

      1390       1400       1410       1420       1430       1440 
IKLVNDKAVA TSQLQKKLGQ LLYLTNLEKS QDKTSGGINP EPCPICARQL GKQWAVLTCG 

      1450       1460       1470       1480       1490       1500 
HCFCNECTSI IIEQYSVGSH RSSIKCAICR QTTSHKEVSY VFTSEKANQE DDIPVKGSHS 

      1510       1520       1530       1540       1550       1560 
TKVEAVVRTL MKIQLRDPGA KALVFSTWQD VLDIISKALT DNNMEFTQIS RIKTFQENLS 

      1570       1580       1590       1600       1610       1620 
AFKYDPHINI LLLPLHTGSN GLTIIEATHV LLVEPILNPA HELQAIGRVH RIGQTKPTIV 

      1630       1640       1650       1660       1670 
HRFLIKATIE ERMQAMLKTA ERSHTSSSGK HSEASVLTVA GLADLFTKEN EELE 

« Hide

Isoform 2 (A) [UniParc].

Checksum: 64D639BE774E29B6
Show »

FASTA1,616185,068
Isoform 3 [UniParc].

Checksum: 08CB942E51F9AB41
Show »

FASTA1,031118,054
Isoform 4 (C) [UniParc].

Checksum: 02C9E8FD7E75B2E5
Show »

FASTA1,646188,535

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel gene, SHPRH, encoding a conserved putative protein with SNF2/helicase and PHD-finger domains from the 6q24 region."
Sood R., Makalowska I., Galdzicki M., Hu P., Eddings E., Robbins C.M., Moses T., Namkoong J., Chen S., Trent J.M.
Genomics 82:153-161(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 4), TISSUE SPECIFICITY.
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Cerebellum and Eye.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6 and FVB/N.
Tissue: Mammary tumor and Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY162264 mRNA. Translation: AAO26654.1.
AY162265 mRNA. Translation: AAO26655.1. Sequence problems.
AY162266 mRNA. Translation: AAO26656.1.
AK053448 mRNA. Translation: BAC35389.1.
AK082160 mRNA. Translation: BAC38428.1.
BC006883 mRNA. Translation: AAH06883.1.
BC055003 mRNA. Translation: AAH55003.1.
RefSeqNP_001071175.1. NM_001077707.1.
NP_001271283.1. NM_001284354.1.
NP_766525.3. NM_172937.3.
XP_006512803.1. XM_006512740.1.
UniGeneMm.133101.

3D structure databases

ProteinModelPortalQ7TPQ3.
SMRQ7TPQ3. Positions 645-707, 709-980, 1421-1469, 1506-1672.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid234474. 5 interactions.

PTM databases

PhosphoSiteQ7TPQ3.

Proteomic databases

PaxDbQ7TPQ3.
PRIDEQ7TPQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044053; ENSMUSP00000039422; ENSMUSG00000090112. [Q7TPQ3-1]
ENSMUST00000054814; ENSMUSP00000125849; ENSMUSG00000090112. [Q7TPQ3-2]
ENSMUST00000159541; ENSMUSP00000132870; ENSMUSG00000090112. [Q7TPQ3-5]
ENSMUST00000159810; ENSMUSP00000125457; ENSMUSG00000090112. [Q7TPQ3-3]
GeneID268281.
KEGGmmu:268281.
UCSCuc007ejp.1. mouse. [Q7TPQ3-1]

Organism-specific databases

CTD257218.
MGIMGI:1917581. Shprh.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00730000111123.
HOVERGENHBG059171.
InParanoidQ7TPQ3.
KOK15710.
OMAFEGLVKQ.
OrthoDBEOG7ZD1TJ.
PhylomeDBQ7TPQ3.
TreeFamTF324273.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ7TPQ3.
BgeeQ7TPQ3.
CleanExMM_SHPRH.
GenevestigatorQ7TPQ3.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.30.40.10. 2 hits.
3.40.50.300. 4 hits.
InterProIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR005818. Histone_H1/H5_H15.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011991. WHTH_DNA-bd_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00271. Helicase_C. 1 hit.
PF00538. Linker_histone. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00526. H15. 1 hit.
SM00249. PHD. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 6 hits.
SSF57903. SSF57903. 1 hit.
PROSITEPS51504. H15. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSHPRH. mouse.
NextBio392208.
PROQ7TPQ3.
SOURCESearch...

Entry information

Entry nameSHPRH_MOUSE
AccessionPrimary (citable) accession number: Q7TPQ3
Secondary accession number(s): Q7TQ27 expand/collapse secondary AC list , Q7TQ28, Q7TQ29, Q8BKE2, Q8BUW0, Q922Q3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot